Strain identifier

BacDive ID: 10436

Type strain: No

Species: Aquaspirillum serpens

Strain Designation: Kh-4

Strain history: CIP <- 1998, ATCC <- A. Banerjee: strain Kh-4

NCBI tax ID(s): 190 (species)

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General

@ref: 3492

BacDive-ID: 10436

DSM-Number: 9156

keywords: 16S sequence, Bacteria, Gram-negative, motile, rod-shaped

description: Aquaspirillum serpens Kh-4 is a Gram-negative, motile, rod-shaped bacterium that was isolated from freshwater pond.

NCBI tax id

  • NCBI tax id: 190
  • Matching level: species

strain history

@refhistory
3492<- ATCC <- A. Banerjee, Kh-4
67770IAM 14967 <-- IFO 15465 <-- ATCC 27641 <-- A. Banerjee Kh-4.
122573CIP <- 1998, ATCC <- A. Banerjee: strain Kh-4

doi: 10.13145/bacdive10436.20240510.9

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Betaproteobacteria
  • order: Neisseriales
  • family: Chromobacteriaceae
  • genus: Aquaspirillum
  • species: Aquaspirillum serpens
  • full scientific name: Aquaspirillum serpens (Müller 1786) Hylemon et al. 1973 (Approved Lists 1980)

  • synonyms

    • @ref: 20215
    • synonym: Vibrio serpens

@ref: 3492

domain: Bacteria

phylum: Proteobacteria

class: Betaproteobacteria

order: Neisseriales

family: Chromobacteriaceae

genus: Aquaspirillum

species: Aquaspirillum serpens

full scientific name: Aquaspirillum serpens (Müller 1786) Hylemon et al. 1973 emend. Boivin et al. 1985

strain designation: Kh-4

type strain: no

Morphology

cell morphology

  • @ref: 122573
  • gram stain: negative
  • cell shape: rod-shaped
  • motility: yes

Culture and growth conditions

culture medium

@refnamegrowthcompositionlink
39360MEDIUM 14 - Williams and Rittenberg medium for Aquaspirillum genusyesDistilled water make up to (1000.000 ml);Agar (13.000 g);Yeast extract (3.000 g);Peptone (5.000 g);Beef extract (3.000 g)
3492PEPTONE-SUCCINATE AGAR (DSMZ Medium 653)yesName: PEPTONE-SUCCINATE AGAR (DSMZ Medium 653) Composition: Peptone 5.0 g/l Succinic acid 1.68 g/l Agar 1.5 g/l MgSO4 x 7 H2O 1.0 g/l (NH4)2SO4 1.0 g/l FeCl3 x 6 H2O 0.002 g/l MnSO4 x H2O 0.002 g/l Distilled waterhttps://mediadive.dsmz.de/medium/653
122573CIP Medium 14yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=14

culture temp

@refgrowthtypetemperature
39360positivegrowth25
3492positivegrowth30
67770positivegrowth30
122573positivegrowth15-41
122573nogrowth5

Physiology and metabolism

halophily

@refsaltgrowthtested relationconcentration
122573NaClpositivegrowth0 %
122573NaClnogrowth2 %
122573NaClnogrowth4 %
122573NaClnogrowth6 %
122573NaClnogrowth8 %
122573NaClnogrowth10 %

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
12257316947citrate-carbon source
1225734853esculin-hydrolysis
12257317234glucose-fermentation
12257317716lactose-fermentation
12257317632nitrate-reduction
12257316301nitrite-reduction
122573132112sodium thiosulfate-builds gas from
12257317234glucose-degradation
12257317632nitrate-respiration

antibiotic resistance

  • @ref: 122573
  • metabolite: 0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)
  • is antibiotic: yes
  • is sensitive: yes
  • is resistant: no

metabolite production

  • @ref: 122573
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testmethylred-test
12257315688acetoin-
12257317234glucose-

enzymes

@refvalueactivityec
122573oxidase+
122573beta-galactosidase-3.2.1.23
122573alcohol dehydrogenase-1.1.1.1
122573gelatinase-
122573catalase+1.11.1.6
122573gamma-glutamyltransferase+2.3.2.2
122573lysine decarboxylase-4.1.1.18
122573ornithine decarboxylase-4.1.1.17
122573phenylalanine ammonia-lyase-4.3.1.24
122573tryptophan deaminase-
122573urease-3.5.1.5
68382alkaline phosphatase-3.1.3.1
68382esterase (C 4)+
68382esterase lipase (C 8)+
68382lipase (C 14)-
68382leucine arylamidase+3.4.11.1
68382valine arylamidase-
68382cystine arylamidase-3.4.11.3
68382trypsin-3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase-3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase-3.2.1.20
68382beta-glucosidase-3.2.1.21
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
122573--++-+----++--------

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
122573-------------------------------------------------------------------------+---------------++--------

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinent
3492freshwater pond
67770Freshwater pondWest BengalIndiaINDAsia
122573Environment, Freshwater pondWest BengalIndiaINDAsia

isolation source categories

Cat1Cat2Cat3
#Environmental#Aquatic#Freshwater
#Environmental#Aquatic#Pond (small)

taxonmaps

  • @ref: 69479
  • File name: preview.99_77397.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_15355;96_23870;97_29864;98_38181;99_77397&stattab=map
  • Last taxonomy: Aquaspirillum serpens subclade
  • 16S sequence: AB680863
  • Sequence Identity:
  • Total samples: 11827
  • soil counts: 408
  • aquatic counts: 9687
  • animal counts: 1624
  • plant counts: 108

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
34921Risk group (German classification)
1225731Risk group (French classification)

Sequence information

16S sequences

  • @ref: 20218
  • description: Aquaspirillum serpens gene for 16S rRNA, partial sequence, strain: NBRC 15465
  • accession: AB680863
  • length: 1461
  • database: nuccore
  • NCBI tax ID: 190

GC content

  • @ref: 67770
  • GC-content: 51
  • method: thermal denaturation, midpoint method (Tm)

External links

@ref: 3492

culture collection no.: DSM 9156, ATCC 27641, IFO 15465, NBRC 15465, JCM 21439, CIP 105799, IAM 14967, LMG 6234

straininfo link

  • @ref: 79717
  • straininfo: 13576

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
3492Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 9156)https://www.dsmz.de/collection/catalogue/details/culture/DSM-9156
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
39360Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/17858
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
79717Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID13576.1StrainInfo: A central database for resolving microbial strain identifiers
122573Curators of the CIPCollection of Institut Pasteur (CIP 105799)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20105799