Alysiella crassa (DSM 2578, ATCC 15533, NCTC 10283)

Name: Alysiella crassa (DSM 2578, ATCC 15533, NCTC 10283)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 10433

Type strain

Strain Designation S6

Culture col. no. DSM 2578 ATCC 15533 NCTC 10283 CCUG 25927 S6 6 more

NCBI tax ID(s) 1120980 153491

Special Collections DSMZ CCUG JCM CIP

History DSMZ <- ATCC <- NCTC <- P. Steed, S6 JCM <-- IAM 14969 <-- ATCC 15533 <-- NCTC 10283 <-- P. Steed S6. CIP <- 1989, NCTC <- 1962, P.D.M. Steed, Brisbane, UK: strain S6

Links

Alysiella crassa S6 is a bacterium that was isolated from sheep saliva.

genome sequence 16S sequence Bacteria

Name and taxonomic classification

SI-ID 4972

LMG 7829,ATCC 15533,DSM 2578,NCTC 10283,CCUG 25927,CIP 103341,IAM 14969,JCM 21440,NBRC 102455

@ref 20215

Last LPSN update 2026-05-29 08:27:20

Domain Bacteria

Phylum Pseudomonadota

Class Betaproteobacteria

Order Neisseriales

Family Neisseriaceae

Genus Alysiella

Species Alysiella crassa

Full scientific name Alysiella crassa (Schmid 1922) Xie and Yokota 2005

Synonyms (1)

Simonsiella crassa

Morphology

Cell morphology

@ref	Gram stain	Confidence	Motility
125438	negative	97.554
125439		92.96

Multimedia

@ref:	66793
multimedia content:	EM_DSM_2578_1.jpg
multimedia.multimedia content:	EM_DSM_2578_1.jpg
caption:	electron microscopic image
intellectual property rights:	© HZI/Manfred Rohde
manual_annotation:	1

@ref:	66793
multimedia content:	EM_DSM_2578_2.jpg
multimedia.multimedia content:	EM_DSM_2578_2.jpg
caption:	electron microscopic image
intellectual property rights:	© HZI/Manfred Rohde
manual_annotation:	1

@ref:	66793
multimedia content:	EM_DSM_2578_3.jpg
multimedia.multimedia content:	EM_DSM_2578_3.jpg
caption:	electron microscopic image
intellectual property rights:	© HZI/Manfred Rohde
manual_annotation:	1

@ref:	66793
multimedia content:	EM_DSM_2578_4.jpg
multimedia.multimedia content:	EM_DSM_2578_4.jpg
caption:	electron microscopic image
intellectual property rights:	© HZI/Manfred Rohde
manual_annotation:	1

@ref:	66793
multimedia content:	EM_DSM_2578_5.jpg
multimedia.multimedia content:	EM_DSM_2578_5.jpg
caption:	electron microscopic image
intellectual property rights:	© HZI/Manfred Rohde
manual_annotation:	1

Culture and growth conditions

Culture medium

@ref	Name	Medium link	Composition
1128	TRYPTONE SOYA BROTH (TSB) (DSMZ Medium 545)	Medium recipe at MediaDive	Name: TRYPTONE SOYA BROTH (TSB) (DSMZ Medium 545) Composition: Casein peptone 17.0 g/l NaCl 5.0 g/l Soy peptone 3.0 g/l D(+)-Glucose 2.5 g/l K2HPO4 2.5 g/l Distilled water
1128	COLUMBIA BLOOD AGAR (DSMZ Medium 429)	Medium recipe at MediaDive	Name: COLUMBIA BLOOD AGAR (DSMZ Medium 429) Composition: Horse blood 40.0 g/l Columbia agar base
1128	NUTRIENT BROTH WITH 10% HORSE SERUM (DSMZ Medium 302)	Medium recipe at MediaDive	Name: NUTRIENT BROTH WITH 10% HORSE SERUM (DSMZ Medium 302) Composition: Agar 15.0 g/l Peptone 5.0 g/l Meat extract 3.0 g/l Horse serum Distilled water
38126	MEDIUM 6 - Columbia agar with 10 % horse blood		Distilled water make up to (1000.000 ml);Columbia agar (39.000 g);Horseblood (100.000 ml)
118776	CIP Medium 6	Medium recipe at CIP

Culture temp

@ref	Growth	Type	Temperature (°C)
1128	positive	growth	37
38126	positive	growth	37
67770	positive	growth	37

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance	Confidence
125439	obligate aerobe	94.336

Spore formation

@ref	Spore formation	Confidence
125438		91.848
125439		99.419

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
68377	15824 ChEBI	D-fructose	-	builds acid from	from API NH
68377	17634 ChEBI	D-glucose	+	builds acid from	from API NH
68377	17306 ChEBI	maltose	-	builds acid from	from API NH
68377	18257 ChEBI	ornithine	+	degradation	from API NH
68377	17992 ChEBI	sucrose	-	builds acid from	from API NH
68377	27897 ChEBI	tryptophan	-	energy source	from API NH
68377	16199 ChEBI	urea	-	hydrolysis	from API NH

Metabolite production

@ref	Chebi-ID	Metabolite	Production
68377	35581 ChEBI	indole		from API NH

Metabolite tests

@ref	Chebi-ID	Metabolite	Indole test
68377	35581 ChEBI	indole	-	from API NH

Enzymes

@ref	Value	Activity	Ec
68382	acid phosphatase	+	3.1.3.2	from API zym
68382	alkaline phosphatase	-	3.1.3.1	from API zym
68377	alkaline phosphatase	-	3.1.3.1	from API NH
68382	alpha-chymotrypsin	-	3.4.21.1	from API zym
68382	alpha-fucosidase	-	3.2.1.51	from API zym
68382	alpha-galactosidase	-	3.2.1.22	from API zym
68382	alpha-glucosidase	-	3.2.1.20	from API zym
68382	alpha-mannosidase	-	3.2.1.24	from API zym
68382	beta-galactosidase	-	3.2.1.23	from API zym
68377	beta-galactosidase	-	3.2.1.23	from API NH
68382	beta-glucosidase	-	3.2.1.21	from API zym
68382	beta-glucuronidase	-	3.2.1.31	from API zym
68377	beta-lactamase	-	3.5.2.6	from API NH
68382	cystine arylamidase	-	3.4.11.3	from API zym
68382	esterase (C 4)	+		from API zym
68382	esterase lipase (C 8)	+		from API zym
68377	gamma-glutamyltransferase	-	2.3.2.2	from API NH
68382	leucine arylamidase	+	3.4.11.1	from API zym
68377	lipase	+		from API NH
68382	lipase (C 14)	-		from API zym
68382	N-acetyl-beta-glucosaminidase	-	3.2.1.52	from API zym
68382	naphthol-AS-BI-phosphohydrolase	-		from API zym
68377	ornithine decarboxylase	+	4.1.1.17	from API NH
68377	proline-arylamidase	-	3.4.11.5	from API NH
68382	trypsin	-	3.4.21.4	from API zym
68377	tryptophan deaminase	-	4.1.99.1	from API NH
68377	urease	-	3.5.1.5	from API NH
68382	valine arylamidase	-		from API zym

Pathway annotation

Computationally determined by

@ref	pathway	enzyme coverage	annotated reactions
66794	coenzyme A metabolism	100	4 of 4
66794	reductive acetyl coenzyme A pathway	100	7 of 7
66794	cis-vaccenate biosynthesis	100	2 of 2
66794	formaldehyde oxidation	100	3 of 3
66794	denitrification	100	2 of 2
66794	ubiquinone biosynthesis	100	7 of 7
66794	CDP-diacylglycerol biosynthesis	100	2 of 2
66794	palmitate biosynthesis	100	22 of 22
66794	UDP-GlcNAc biosynthesis	100	3 of 3
66794	ppGpp biosynthesis	100	4 of 4
66794	anapleurotic synthesis of oxalacetate	100	1 of 1
66794	folate polyglutamylation	100	1 of 1
66794	pentose phosphate pathway	90.91	10 of 11
66794	threonine metabolism	90	9 of 10
66794	valine metabolism	88.89	8 of 9
66794	lipid A biosynthesis	88.89	8 of 9
66794	molybdenum cofactor biosynthesis	88.89	8 of 9
66794	chorismate metabolism	88.89	8 of 9
66794	isoleucine metabolism	87.5	7 of 8
66794	photosynthesis	85.71	12 of 14
66794	propanol degradation	85.71	6 of 7
66794	methylglyoxal degradation	80	4 of 5
66794	peptidoglycan biosynthesis	80	12 of 15
66794	heme metabolism	78.57	11 of 14
66794	tetrahydrofolate metabolism	78.57	11 of 14
66794	vitamin B1 metabolism	76.92	10 of 13
66794	phenylalanine metabolism	76.92	10 of 13
66794	lactate fermentation	75	3 of 4
66794	glycogen biosynthesis	75	3 of 4
66794	6-hydroxymethyl-dihydropterin diphosphate biosynthesis	75	6 of 8
66794	CMP-KDO biosynthesis	75	3 of 4
66794	C4 and CAM-carbon fixation	75	6 of 8
66794	gluconeogenesis	75	6 of 8
66794	flavin biosynthesis	73.33	11 of 15
66794	citric acid cycle	71.43	10 of 14
66794	cardiolipin biosynthesis	71.43	5 of 7
66794	Entner Doudoroff pathway	70	7 of 10
66794	octane oxidation	66.67	2 of 3
66794	aspartate and asparagine metabolism	66.67	6 of 9
66794	L-lactaldehyde degradation	66.67	2 of 3
66794	NAD metabolism	66.67	12 of 18
66794	acetoin degradation	66.67	2 of 3
66794	cyanate degradation	66.67	2 of 3
66794	glutathione metabolism	64.29	9 of 14
66794	proline metabolism	63.64	7 of 11
66794	ketogluconate metabolism	62.5	5 of 8
66794	isoprenoid biosynthesis	61.54	16 of 26
66794	methionine metabolism	61.54	16 of 26
66794	cysteine metabolism	61.11	11 of 18
66794	lipoate biosynthesis	60	3 of 5
66794	hydrogen production	60	3 of 5
66794	glycogen metabolism	60	3 of 5
66794	propionate fermentation	60	6 of 10
66794	glycolysis	58.82	10 of 17
66794	purine metabolism	56.38	53 of 94
66794	CO2 fixation in Crenarchaeota	55.56	5 of 9
66794	d-mannose degradation	55.56	5 of 9
66794	non-pathway related	55.26	21 of 38
66794	alanine metabolism	55.17	16 of 29
66794	vitamin B6 metabolism	54.55	6 of 11
66794	urea cycle	53.85	7 of 13
66794	leucine metabolism	53.85	7 of 13
66794	glycolate and glyoxylate degradation	50	3 of 6
66794	glutamate and glutamine metabolism	50	14 of 28
66794	acetate fermentation	50	2 of 4
66794	dTDPLrhamnose biosynthesis	50	4 of 8
66794	suberin monomers biosynthesis	50	1 of 2
66794	adipate degradation	50	1 of 2
66794	ribulose monophosphate pathway	50	1 of 2
66794	sulfopterin metabolism	50	2 of 4
66794	butanoate fermentation	50	2 of 4
66794	ethanol fermentation	50	1 of 2
66794	lipid metabolism	48.39	15 of 31
66794	histidine metabolism	48.28	14 of 29
66794	pyrimidine metabolism	46.67	21 of 45
66794	phenylpropanoid biosynthesis	46.15	6 of 13
66794	sulfate reduction	46.15	6 of 13
66794	d-xylose degradation	45.45	5 of 11
66794	lysine metabolism	45.24	19 of 42
66794	serine metabolism	44.44	4 of 9
66794	tyrosine metabolism	42.86	6 of 14
66794	tryptophan metabolism	42.11	16 of 38
66794	arginine metabolism	41.67	10 of 24
66794	degradation of aromatic, nitrogen containing compounds	41.67	5 of 12
66794	degradation of sugar acids	40	10 of 25
66794	gallate degradation	40	2 of 5
66794	3-chlorocatechol degradation	40	2 of 5
66794	starch degradation	40	4 of 10
66794	arachidonate biosynthesis	40	2 of 5
66794	oxidative phosphorylation	39.56	36 of 91
66794	degradation of pentoses	39.29	11 of 28
66794	degradation of sugar alcohols	37.5	6 of 16
66794	polyamine pathway	34.78	8 of 23
66794	acetyl CoA biosynthesis	33.33	1 of 3
66794	pantothenate biosynthesis	33.33	2 of 6
66794	selenocysteine biosynthesis	33.33	2 of 6
66794	IAA biosynthesis	33.33	1 of 3
66794	degradation of hexoses	33.33	6 of 18
66794	enterobactin biosynthesis	33.33	1 of 3
66794	nitrate assimilation	33.33	3 of 9
66794	(5R)-carbapenem carboxylate biosynthesis	33.33	1 of 3
66794	ascorbate metabolism	31.82	7 of 22
66794	carotenoid biosynthesis	31.82	7 of 22
66794	glycine metabolism	30	3 of 10
66794	cholesterol biosynthesis	27.27	3 of 11
66794	dolichyl-diphosphooligosaccharide biosynthesis	27.27	3 of 11
66794	metabolism of disaccharids	27.27	3 of 11
66794	3-phenylpropionate degradation	26.67	4 of 15
66794	alginate biosynthesis	25	1 of 4
66794	androgen and estrogen metabolism	25	4 of 16
66794	cyclohexanol degradation	25	1 of 4
66794	toluene degradation	25	1 of 4
66794	phenol degradation	25	5 of 20
66794	biotin biosynthesis	25	1 of 4
66794	phosphatidylethanolamine bioynthesis	23.08	3 of 13
66794	allantoin degradation	22.22	2 of 9
66794	arachidonic acid metabolism	22.22	4 of 18

Fatty acid profile

Metadata FA analysis

@ref

48297

Fatty acid	Percentage	ECL
C18:1 ω7c /12t/9t	30.7	17.824
C14:0	22.3	14
C16:1 ω7c	21.3	15.819
C16:0	12.4	16
C12:0	4.2	12
C12:0 3OH	2.3	13.455
C18:2 ω6,9c/C18:0 ANTE	1.9	17.724
C13:0 ISO 2OH	1.7	13.814
C14:0 3OH/C16:1 ISO I	1.2	15.485
C18:0	1.2	18
C18:1 ω9c	0.9	17.769

API zym

@ref	Control	Alkaline phosphatase	Esterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	Lipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	Trypsin	alpha- Chymotrypsin	Acid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	alpha- Galactosidase	beta- Galactosidase	beta- Glucuronidase	alpha- Glucosidase	beta- Glucosidase	N-acetyl-beta- glucosaminidase	alpha- Mannosidase	alpha- Fucosidase
48297	-	-	+	+	-	+	-	-	-	-	+	-	-	-	-	-	-	-	-	-

API NH

@ref	PEN	GLU	FRU	MAL	SAC	ODC	URE	LIP	PAL	beta GAL	ProA	GGT	IND
48297	-	+	-	-	-	+	-	+	-	-	-	-	-

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Host	#Mammals	#Caprinae (Sheep/Goat)
#Host Body Product	#Oral cavity and Airways	#Saliva

Isolation

@ref	Sample type	Country	Country ISO 3 Code	Continent
1128	sheep saliva	Australia	AUS	Australia and Oceania
48297	Sheep saliva
67770	Sheep saliva
118776	Animal, Sheep, saliva	Australia	AUS	Australia and Oceania

Taxonmaps

69479

Global distribution of 16S sequence AB087264 (>99% sequence identity) for Alysiella crassa from Microbeatlas

Aquatic Samples	294
Soil Samples	9
Animal Samples	1638
Plant Samples	24
Total Samples	1965

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
1128	1	Risk group (German classification) According to TRBA 466
118776	1	Risk group (French classification) According to TRBA 466

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
66792	ASM2287104v2 assembly for Alysiella crassa S6	complete	GCA_022871045	153491.5	8063117508	153491	92.5
67770	52756_C01 assembly for Alysiella crassa NCTC10283	contig	GCA_900445245	153491.3	2808606660	153491	77.66
67770	ASM74595v1 assembly for Alysiella crassa DSM 2578	scaffold	GCA_000745955	1120980.3	2571042010	1120980	46.64

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
20218	Alysiella crassa gene for 16S rRNA, strain: IAM 14969	AB087264	1492		153491
20218	Alysiella crassa gene for 16S rRNA, partial sequence, strain: NBRC 102455	AB681797	1462		153491

GC content

1128

GC-content (mol%)43.9

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: ASM2287104v2 assembly for Alysiella crassa S6
NCBI: GCA_022871045

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	oxygen_tolerance	BacteriaNetⓘ	obligate aerobe	94.34	no
125439	gram_stain	BacteriaNetⓘ	positive	46.65	no
125439	motility	BacteriaNetⓘ	no	92.96	no
125439	spore_formation	BacteriaNetⓘ	no	99.42	no

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Alysiella crassa S6
NCBI: GCA_022871045.1

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	no	97.55	no
125438	anaerobic	anaerobicⓘ	no	92.60	no
125438	aerobic	aerobicⓘ	no	51.88	no
125438	spore-forming	spore-formingⓘ	no	91.85	no
125438	thermophilic	thermophileⓘ	no	96.96	yes
125438	flagellated	motile2+ⓘ	no	81.93	no

Literature

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Identification of integrative and conjugative elements in pathogenic and commensal Neisseriaceae species via genomic distributions of DNA uptake sequence dialects.	Hughes-Games A, Roberts AP, Davis SA, Hill DJ.	Microb Genom	10.1099/mgen.0.000372	2020
Metabolism	Bioconversion of dilute-acid pretreated sorghum bagasse to ethanol by Neurospora crassa.	Dogaris I, Gkounta O, Mamma D, Kekos D.	Appl Microbiol Biotechnol	10.1007/s00253-012-4113-1	2012
Enzymology	Fungal multienzyme production on industrial by-products of the citrus-processing industry.	Mamma D, Kourtoglou E, Christakopoulos P.	Bioresour Technol	10.1016/j.biortech.2007.05.018	2008
Enzymology	New real-time PCR-based method for Kingella kingae DNA detection: application to samples collected from 89 children with acute arthritis.	Ilharreborde B, Bidet P, Lorrot M, Even J, Mariani-Kurkdjian P, Liguori S, Vitoux C, Lefevre Y, Doit C, Fitoussi F, Pennecot G, Bingen E, Mazda K, Bonacorsi S.	J Clin Microbiol	10.1128/jcm.00144-09	2009
Phylogeny	Isolation, characterization, and numerical taxonomy of Simonsiella strains from the oral cavities of cats, dogs, sheep, and humans.	Kuhn DA, Gregory DA, Buchanan GE Jr, Nyby MD, Daly KR	Arch Microbiol	10.1007/BF00429111	1978
Phylogeny	Paralysiella testudinis gen. nov., sp. nov., isolated from the cloaca of a toad-headed turtle (Mesoclemmys nasuta).	Busse HJ, Kampfer P, Szostak MP, Ruckert C, Spergser J	Int J Syst Evol Microbiol	10.1099/ijsem.0.005114	2021

References

#1128	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 2578
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#20218	Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )
#38126	; Curators of the CIP;
#48297	Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 25927
#66792	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .
#66793	Mukherjee et al.: GEBA: 1,003 reference genomes of bacterial and archaeal isolates expand coverage of the tree of life. 35: 676 - 683 2017 ( DOI 10.1038/nbt.3886 , PubMed 28604660 )
#66794	Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg: BRENDA, the ELIXIR core data resource in 2021: new developments and updates. Nucleic Acids Res. 49: D498 - D508 2020 ( DOI 10.1093/nar/gkaa1025 , PubMed 33211880 )
#67770	Japan Collection of Microorganism (JCM) ; Curators of the JCM;
#68377	Automatically annotated from API NH .
#68382	Automatically annotated from API zym .
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#118776	Collection of Institut Pasteur ; Curators of the CIP; CIP 103341
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Alysiella crassa (DSM 2578, ATCC 15533, NCTC 10283)

Name and taxonomic classification

Morphology

Cell morphology

Multimedia

Culture and growth conditions

Culture medium

Culture temp

Physiology and metabolism

Oxygen tolerance

Spore formation

Metabolite utilization

Metabolite production

Metabolite tests

Enzymes

Pathway annotation

Fatty acid profile

API zym

API NH

Isolation, sampling and environmental information

Isolation source categories

Isolation

Taxonmaps

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_022871045

16S sequences

GC content

Genome-based predictions

Predictions

Literature

Literature

References

BacDive

Help

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