Name: Komagataeibacter rhaeticus DSM 16663
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 94

Type strain:

Species: Komagataeibacter rhaeticus

Culture col. no.: DSM 16663, BCC 36452, JCM 17122, LMG 22126, DST GL02, CIP 109761

Strain history: CIP <- 2007, F. Dellaglio, Verona Univ., Verona, Italy, strain: DST GL02

NCBI tax ID(s): 1307939 (strain), 215221 (species)

SI-ID 128067

LMG 22126, DSM 16663, CIP 109761, BCC 36452, JCM 17122

Section

Komagataeibacter rhaeticus DSM 16663 is a mesophilic, Gram-negative, rod-shaped bacterium that was isolated from organic apple juice.

Gram-negative
rod-shaped
mesophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Pseudomonadota*
	Class	*Alphaproteobacteria*
	Order	*Rhodospirillales*
	Family	*Acetobacteraceae*
	Genus	*Komagataeibacter*
	Species	**Komagataeibacter rhaeticus**
	Full Scientific Name (LPSN)	Komagataeibacter rhaeticus (Dellaglio et al. 2005) Yamada et al. 2013

	Synonym	"Komagatabacter* rhaeticus"*
	Synonym	Gluconacetobacter rhaeticus

Information on morphological and physiological properties Morphology

[Ref.: #122180]	Gram stain	negative

[Ref.: #122180]	Cell shape	rod-shaped

[Ref.: #122180]	Motility	no

Information on culture and growth conditions Culture and growth conditions

[Ref.: #6558]

Culture medium

GLUCONACETOBACTER RHAETICUS MEDIUM (DSMZ Medium 1044)

[Ref.: #6558]

Culture medium growth

[Ref.: #6558]

Culture medium link

Medium recipe at MediaDive

[Ref.: #6558]

Culture medium composition

expand / minimize

[Ref.: #33078]

Culture medium

MEDIUM 1 - for Acetobacter, Azotobacter, Gluconobacter, Gluconacetobacter, Mesorhizodium ciceri and Pseudomonas doudoroffii

[Ref.: #33078]

Culture medium growth

[Ref.: #33078]

Culture medium composition

expand / minimize

[Ref.: #122180]

Culture medium

CIP Medium 1

[Ref.: #122180]

Culture medium growth

[Ref.: #122180]

Culture medium link

Medium recipe provided by DSMZ

		Temperature
[Ref.: #6558]	growth	28 °C
[Ref.: #33078]	growth	30 °C
[Ref.: #67770]	growth	28 °C

[Ref.: #6558]	Temperature range	mesophilic
[Ref.: #33078]	Temperature range	mesophilic
[Ref.: #67770]	Temperature range	mesophilic

Information on physiology and metabolism Physiology and metabolism

[Ref.: #67770]

Observation

quinones: Q-10

	Metabolite utilization	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #122180]		nitrate ChEBI	-	reduction
[Ref.: #122180]		nitrite ChEBI	-	reduction

	Metabolite production	Metabolite	Production
[Ref.: #122180]		indole ChEBI	no

	Enzyme	Enzyme activity	EC number
[Ref.: #68382]	acid phosphatase	+	3.1.3.2	* from API zym
[Ref.: #68382]	alkaline phosphatase	-	3.1.3.1	* from API zym
[Ref.: #68382]	alpha-chymotrypsin	-	3.4.21.1	* from API zym
[Ref.: #68382]	alpha-fucosidase	-	3.2.1.51	* from API zym
[Ref.: #68382]	alpha-galactosidase	-	3.2.1.22	* from API zym
[Ref.: #68382]	alpha-glucosidase	+	3.2.1.20	* from API zym
[Ref.: #68382]	alpha-mannosidase	-	3.2.1.24	* from API zym
[Ref.: #68382]	beta-galactosidase	-	3.2.1.23	* from API zym
[Ref.: #68382]	beta-glucosidase	+	3.2.1.21	* from API zym
[Ref.: #68382]	beta-glucuronidase	-	3.2.1.31	* from API zym
[Ref.: #122180]	catalase	+	1.11.1.6
[Ref.: #68382]	cystine arylamidase	+	3.4.11.3	* from API zym
[Ref.: #68382]	esterase (C 4)	+		* from API zym
[Ref.: #68382]	esterase lipase (C 8)	+		* from API zym
[Ref.: #68382]	leucine arylamidase	+	3.4.11.1	* from API zym
[Ref.: #68382]	lipase (C 14)	-		* from API zym
[Ref.: #68382]	N-acetyl-beta-glucosaminidase	-	3.2.1.52	* from API zym
[Ref.: #68382]	naphthol-AS-BI-phosphohydrolase	+		* from API zym
[Ref.: #122180]	oxidase	-
[Ref.: #68382]	trypsin	+	3.4.21.4	* from API zym
[Ref.: #122180]	urease	-	3.5.1.5
[Ref.: #68382]	valine arylamidase	+		* from API zym
	Only first 10 entries are displayed. Click here to see all.

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #122180]	12094	-	-	+	+	-	+	+	+	+	-	+	+	-	-	-	+	+	-	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #6558]	Sample type/isolated from	organic apple juice
[Ref.: #6558]	Geographic location (country and/or sea, region)	South Tyrol, Val Venosta
[Ref.: #6558]	Country	Italy
[Ref.: #6558]	Country ISO 3 Code	ITA
[Ref.: #6558]	Continent	Europe

[Ref.: #67770]	Sample type/isolated from	Apple juice in South Tyrol region
[Ref.: #67770]	Country	Italy
[Ref.: #67770]	Country ISO 3 Code	ITA
[Ref.: #67770]	Continent	Europe

[Ref.: #122180]	Sample type/isolated from	Food, Organic apple juice
[Ref.: #122180]	Geographic location (country and/or sea, region)	Val Venosta, south Tyrol
[Ref.: #122180]	Country	Italy
[Ref.: #122180]	Country ISO 3 Code	ITA
[Ref.: #122180]	Continent	Europe
[Ref.: #122180]	Isolation date	2003
* marker position based on {}

Isolation sources categories

#Engineered	#Food production	#Beverage
#Host Body Product	#Plant	#Juice (natural)

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence JF794006 (>99% sequence identity) for Komagataeibacter from Microbeatlas

Aquatic Samples	523
Soil Samples	662
Animal Samples	2327
Plant Samples	555
Total Samples	4067

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #6558]	Biosafety level	1	Risk group (German classification) According to TRBA 466
[Ref.: #122180]	Biosafety level	1	Risk group (French classification)

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #6558]	Gluconacetobacter rhaeticus strain DST GL02 16S ribosomal RNA gene, partial sequence	AY180961	1336	ENA	215221 tax ID
[Ref.: #20218]	Gluconacetobacter rhaeticus strain DSM 16663 16S ribosomal RNA gene, partial sequence	JF794006	1355	ENA	215221 tax ID	*
[Ref.: #20218]	Gluconacetobacter rhaeticus gene for 16S rRNA, partial sequence, strain: JCM 17122	AB645732	1411	ENA	215221 tax ID	*

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #67770]	Komagataeibacter rhaeticus LMG 22126	GCA_003207855	contig	GenBank	215221 tax ID
[Ref.: #66792]	Komagataeibacter rhaeticus strain LMG 22126	215221.10	wgs	PATRIC	215221 tax ID	*
[Ref.: #66792]	Komagataeibacter rhaeticus LMG 22126	2837601869	draft	JGI IMG/M	215221 tax ID	*

[Ref.: #6558]	GC-content	63.4 mol%
[Ref.: #67770]	GC-content	63.4 mol%	high performance liquid chromatography (HPLC)

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Komagataeibacter rhaeticus strain LMG 22126 PATRIC: 215221.10
[Ref.: #69480]	motile	no	84.226	no
[Ref.: #69480]	gram-positive	no	96.198	no
[Ref.: #69480]	anaerobic	no	98.449	no
[Ref.: #69480]	aerobic	yes	85.806	no
[Ref.: #69480]	halophile	no	92.398	no
[Ref.: #69480]	spore-forming	no	96.035	no
[Ref.: #69480]	glucose-util	yes	89.31	no
[Ref.: #69480]	flagellated	no	96.808	no
[Ref.: #69480]	thermophile	no	96.453	yes
[Ref.: #69480]	glucose-ferment	no	86.277	no

Availability in culture collections External links

[Ref.: #6558]

Culture collection no.

DSM 16663, BCC 36452, JCM 17122, LMG 22126, DST GL02, CIP 109761

[Ref.: #69777]

SI-ID 128067

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Description of Gluconacetobacter swingsii sp. nov. and Gluconacetobacter rhaeticus sp. nov., isolated from Italian apple fruit.	Dellaglio F, Cleenwerck I, Felis GE, Engelbeen K, Janssens D, Marzotto M	Int J Syst Evol Microbiol	10.1099/ijs.0.63301-0	2005	*
Metabolism	Characterization of nanocellulose production by strains of Komagataeibacter sp. isolated from organic waste and Kombucha.	Gupte Y, Kulkarni A, Raut B, Sarkar P, Choudhury R, Chawande A, Kumar GRK, Bhadra B, Satapathy A, Das G, Vishnupriya B, Dasgupta S	Carbohydr Polym	10.1016/j.carbpol.2021.118176	2021	*
Biotechnology	Set-Up of Bacterial Cellulose Production From the Genus Komagataeibacter and Its Use in a Gluten-Free Bakery Product as a Case Study.	Vigentini I, Fabrizio V, Dellaca F, Rossi S, Azario I, Mondin C, Benaglia M, Foschino R	Front Microbiol	10.3389/fmicb.2019.01953	2019	*

References

#6558

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 16663

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#20218

Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )

#33078 ; Curators of the CIP;

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#68382

Automatically annotated from API zym .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69777

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID128067.1 )

#122180 Collection of Institut Pasteur ; Curators of the CIP; CIP 109761
* These data were automatically processed and therefore are not curated

Change proposal

You found an error in BacDive? Please tell us about it!

Note that changes will be reviewed and judged. If your changes are legitimate, changes will occur within the next BacDive update. Only proposed changes supported by the according reference will be reviewed. The BacDive team reserves the right to reject proposed changes.

Search for species Komagataeibacter rhaeticus in external resources:
SILVA
BRENDA
PANGAEA
StrainInfo
GBIF

Komagataeibacter rhaeticus strain LMG 22126

Change proposal

Change proposal