Name: Komagataeibacter sucrofermentans DSM 15973
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 95

Type strain:

Species: Komagataeibacter sucrofermentans

Culture col. no.: DSM 15973, BCC 7227, JCM 9730, LMG 18788, BPR 2001, ATCC 700178, BCRC 80162, CECT 7291, CIP 106078

Strain history: CIP <- 1999, JCM <- T. Tsuchida: strain BPR2001

NCBI tax ID(s): 1307942 (strain), 1053551 (species)

SI-ID 12433

LMG 18788, ATCC 700178, JCM 9730, CIP 106078, BCC 7227, BCRC 17108, CCRC 17108, DSM 15973, CECT 7291

Section

Komagataeibacter sucrofermentans DSM 15973 is an obligate aerobe, mesophilic, Gram-negative bacterium that produces polysaccharides and was isolated from black cherry.

polysaccharide production
Gram-negative
mesophilic
obligate aerobe
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Pseudomonadota*
	Class	*Alphaproteobacteria*
	Order	*Rhodospirillales*
	Family	*Acetobacteraceae*
	Genus	*Komagataeibacter*
	Species	**Komagataeibacter sucrofermentans**
	Full Scientific Name (LPSN)	Komagataeibacter sucrofermentans (Toyosaki et al. 1996) Yamada et al. 2013

	Synonym	"Komagatabacter* sucrofermentans"*
	Synonym	Gluconacetobacter sucrofermentans
	Synonym	Acetobacter xylinum subsp. sucrofermentans
	Synonym	Acetobacter xylinus subsp. sucrofermentans

Information on morphological and physiological properties Morphology

[Ref.: #123370]	Gram stain	negative
[Ref.: #69480]	Gram stain	negative Confidence in %99.965

[Ref.: #123370]	Cell shape	rod-shaped

[Ref.: #123370]	Motility	no

Information on culture and growth conditions Culture and growth conditions

[Ref.: #6212]

Culture medium

GLUCONOBACTER OXYDANS MEDIUM (DSMZ Medium 105)

[Ref.: #6212]

Culture medium growth

[Ref.: #6212]

Culture medium link

Medium recipe at MediaDive

[Ref.: #6212]

Culture medium composition

expand / minimize

[Ref.: #39824]

Culture medium

MEDIUM 1 - for Acetobacter, Azotobacter, Gluconobacter, Gluconacetobacter, Mesorhizodium ciceri and Pseudomonas doudoroffii

[Ref.: #39824]

Culture medium growth

[Ref.: #39824]

Culture medium composition

expand / minimize

[Ref.: #123370]

Culture medium

CIP Medium 314

[Ref.: #123370]

Culture medium growth

[Ref.: #123370]

Culture medium link

Medium recipe provided by DSMZ

[Ref.: #123370]

Culture medium

CIP Medium 1

[Ref.: #123370]

Culture medium growth

[Ref.: #123370]

Culture medium link

Medium recipe provided by DSMZ

	Kind of temperature		Temperature
[Ref.: #6212]		growth	28 °C
[Ref.: #39824]		growth	30 °C
[Ref.: #67770]		growth	28 °C
[Ref.: #123370]		growth	22-37 °C
[Ref.: #123370]	no	growth	10 °C
[Ref.: #123370]	no	growth	41 °C
[Ref.: #123370]	no	growth	45 °C

[Ref.: #6212]	Temperature range	mesophilic
[Ref.: #39824]	Temperature range	mesophilic
[Ref.: #67770]	Temperature range	mesophilic
[Ref.: #123370]	Temperature range	psychrophilic
[Ref.: #123370]	Temperature range	thermophilic

Information on physiology and metabolism Physiology and metabolism

[Ref.: #123370]

Oxygen tolerance

obligate aerobe

[Ref.: #69481]	Ability of spore formation	no Confidence in %100
[Ref.: #69480]	Ability of spore formation	no Confidence in %99.996

[Ref.: #6212]

Name of produced compound

cellulose

	Salt	Tested relation		Salt conc.
[Ref.: #123370]	NaCl		growth	0	%
[Ref.: #123370]	NaCl	no	growth	2	%
[Ref.: #123370]	NaCl	no	growth	4	%
[Ref.: #123370]	NaCl	no	growth	6	%
[Ref.: #123370]	NaCl	no	growth	8	%
[Ref.: #123370]	NaCl	no	growth	10	%

[Ref.: #67770]

Observation

quinones: Q-10

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #123370]	hippurate ChEBI	-	hydrolysis
[Ref.: #123370]	nitrate ChEBI	-	reduction
[Ref.: #123370]	nitrite ChEBI	-	reduction

	Antibiotica	Metabolite	Antibiotic sensitivity	Antibiotic resistance
[Ref.: #123370]		0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)	no	yes

	Metabolite production	Metabolite	Production
[Ref.: #67770]		cellulose ChEBI	yes
[Ref.: #123370]		indole ChEBI	no

	Enzyme	Enzyme activity	EC number
[Ref.: #68382]	acid phosphatase	-	3.1.3.2	* from API zym
[Ref.: #68382]	alkaline phosphatase	-	3.1.3.1	* from API zym
[Ref.: #68382]	alpha-chymotrypsin	-	3.4.21.1	* from API zym
[Ref.: #68382]	alpha-fucosidase	-	3.2.1.51	* from API zym
[Ref.: #68382]	alpha-galactosidase	-	3.2.1.22	* from API zym
[Ref.: #68382]	alpha-glucosidase	-	3.2.1.20	* from API zym
[Ref.: #68382]	alpha-mannosidase	-	3.2.1.24	* from API zym
[Ref.: #123370]	beta-galactosidase	-	3.2.1.23
[Ref.: #68382]	beta-galactosidase	-	3.2.1.23	* from API zym
[Ref.: #68382]	beta-glucosidase	-	3.2.1.21	* from API zym
[Ref.: #68382]	beta-glucuronidase	-	3.2.1.31	* from API zym
[Ref.: #123370]	catalase	+	1.11.1.6
[Ref.: #68382]	cystine arylamidase	-	3.4.11.3	* from API zym
[Ref.: #68382]	esterase (C 4)	+		* from API zym
[Ref.: #68382]	esterase lipase (C 8)	+		* from API zym
[Ref.: #123370]	gamma-glutamyltransferase	-	2.3.2.2
[Ref.: #123370]	gelatinase	-
[Ref.: #68382]	leucine arylamidase	-	3.4.11.1	* from API zym
[Ref.: #68382]	lipase (C 14)	-		* from API zym
[Ref.: #68382]	N-acetyl-beta-glucosaminidase	+	3.2.1.52	* from API zym
[Ref.: #68382]	naphthol-AS-BI-phosphohydrolase	+		* from API zym
[Ref.: #123370]	oxidase	-
[Ref.: #68382]	trypsin	-	3.4.21.4	* from API zym
[Ref.: #123370]	urease	-	3.5.1.5
[Ref.: #68382]	valine arylamidase	-		* from API zym
	Only first 10 entries are displayed. Click here to see all.

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #123370]	9233	-	-	+	+	-	-	-	-	-	-	-	+	-	-	-	-	-	+	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #6212]	Sample type/isolated from	black cherry
[Ref.: #6212]	Geographic location (country and/or sea, region)	Tokyo
[Ref.: #6212]	Country	Japan
[Ref.: #6212]	Country ISO 3 Code	JPN
[Ref.: #6212]	Continent	Asia

[Ref.: #67770]	Sample type/isolated from	Cherry

[Ref.: #123370]	Sample type/isolated from	Black cherry
[Ref.: #123370]	Geographic location (country and/or sea, region)	Tokyo
[Ref.: #123370]	Country	Japan
[Ref.: #123370]	Country ISO 3 Code	JPN
[Ref.: #123370]	Continent	Asia
* marker position based on {}

Isolation sources categories

#Host	#Plants	#Tree
#Climate	#Temperate	-

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence AJ007698 (>99% sequence identity) for Komagataeibacter from Microbeatlas

Aquatic Samples	523
Soil Samples	662
Animal Samples	2327
Plant Samples	555
Total Samples	4067

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #6212]	Biosafety level	1	Risk group (German classification) According to TRBA 466
[Ref.: #123370]	Biosafety level	1	Risk group (French classification)

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #6212]	Gluconacetobacter sucrofermentans partial 16S rRNA gene, type strain BPR 2001T	AJ007698	1463	ENA	1307942 tax ID
[Ref.: #20218]	Gluconacetobacter sucrofermentans gene for 16S rRNA, partial sequence, strain: JCM 9730	AB205217	1445	ENA	1307942 tax ID	*
[Ref.: #20218]	Gluconacetobacter sucrofermentans gene for 16S rRNA, partial sequence, strain: JCM 9730	AB645735	1411	ENA	1307942 tax ID	*
[Ref.: #20218]	Gluconacetobacter sucrofermentans gene for 16S rRNA, partial sequence, strain: JCM 9730	AB598742	1411	ENA	1307942 tax ID	*

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #67770]	Komagataeibacter sucrofermentans LMG 18788	GCA_003207865	contig	GenBank	1053551 tax ID
[Ref.: #66792]	Komagataeibacter sucrofermentans strain LMG 18788	1053551.5	wgs	PATRIC	1053551 tax ID	*
[Ref.: #66792]	Komagataeibacter sucrofermentans LMG 18788	2846444558	draft	JGI IMG/M	1053551 tax ID	*

[Ref.: #6212]	GC-content	62.7 mol%	high performance liquid chromatography (HPLC)
[Ref.: #67770]	GC-content	62.3 mol%	high performance liquid chromatography (HPLC)
[Ref.: #67770]	GC-content	62.4 mol%	high performance liquid chromatography (HPLC)

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Komagataeibacter sucrofermentans strain LMG 18788 PATRIC: 1053551.5
[Ref.: #69481]	spore-forming	no	100	no

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Komagataeibacter sucrofermentans strain LMG 18788 PATRIC: 1053551.5
[Ref.: #69480]	motile	no	86.66	no
[Ref.: #69480]	flagellated	no	96.952	no
[Ref.: #69480]	gram-positive	no	97.13	no
[Ref.: #69480]	anaerobic	no	98.593	no
[Ref.: #69480]	aerobic	yes	81.277	no
[Ref.: #69480]	halophile	no	93.132	no
[Ref.: #69480]	spore-forming	no	95.899	no
[Ref.: #69480]	thermophile	no	94.319	no
[Ref.: #69480]	glucose-util	yes	92.958	no
[Ref.: #69480]	glucose-ferment	no	85.774	no

Availability in culture collections External links

[Ref.: #6212]

Culture collection no.

DSM 15973, BCC 7227, JCM 9730, LMG 18788, BPR 2001, ATCC 700178, BCRC 80162, CECT 7291, CIP 106078

[Ref.: #69778]

SI-ID 12433

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Phylogeny and differentiation of species of the genus Gluconacetobacter and related taxa based on multilocus sequence analyses of housekeeping genes and reclassification of Acetobacter xylinus subsp. sucrofermentans as Gluconacetobacter sucrofermentans (Toyosaki et al. 1996) sp. nov., comb. nov.	Cleenwerck I, De Vos P, De Vuyst L	Int J Syst Evol Microbiol	10.1099/ijs.0.018465-0	2009	*
Metabolism	Characterization of purified bacterial cellulose focused on its use on paper restoration.	Santos SM, Carbajo JM, Quintana E, Ibarra D, Gomez N, Ladero M, Eugenio ME, Villar JC	Carbohydr Polym	10.1016/j.carbpol.2014.03.064	2014	*
	Influence of Drying Method and Argon Plasma Modification of Bacterial Nanocellulose on Keratinocyte Adhesion and Growth.	Kutova A, Stankova L, Vejvodova K, Kvitek O, Vokata B, Fajstavr D, Kolska Z, Broz A, Bacakova L, Svorcik V	Nanomaterials (Basel)	10.3390/nano11081916	2021	*
	Synthesis and Characterization of Bacterial Cellulose from Citrus-Based Sustainable Resources.	Andritsou V, de Melo EM, Tsouko E, Ladakis D, Maragkoudaki S, Koutinas AA, Matharu AS	ACS Omega	10.1021/acsomega.8b01315	2018	*

References

#6212

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 15973

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#20218

Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )

#39824 ; Curators of the CIP;

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#68382

Automatically annotated from API zym .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#69778

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID12433.1 )

#123370 Collection of Institut Pasteur ; Curators of the CIP; CIP 106078
* These data were automatically processed and therefore are not curated

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Komagataeibacter sucrofermentans strain LMG 18788

Komagataeibacter sucrofermentans strain LMG 18788

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