Name: Mycobacterium aromaticivorans JS19b1
Creator: BacDive
License: https://creativecommons.org/licenses/by/4.0/

Strain identifier

BacDive ID: 8573

Type strain:

Species: Mycobacterium aromaticivorans

Strain Designation: JS19b1

Culture col. no.: DSM 45407, ATCC BAA-1378, CIP 109274, JCM 16368

Strain history: CIP <- 2006, Q. X. Li, Honolulu, Hawaii, USA: strain JS19b1, "Mycobacterium aromativorans"

NCBI tax ID(s): 1440774 (strain), 318425 (species)

SI-ID 298325

ATCC BAA-1378, CIP 109274, JCM 16368, DSM 45407

Special Collections

Section

Mycobacterium aromaticivorans JS19b1 is an aerobe, Gram-positive, rod-shaped bacterium that was isolated from soil.

Gram-positive
rod-shaped
aerobe
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Actinomycetota*
	Class	*Actinomycetes*
	Order	*Mycobacteriales*
	Family	*Mycobacteriaceae*
	Genus	*Mycobacterium*
	Species	**Mycobacterium aromaticivorans**
	Full Scientific Name (LPSN)	Mycobacterium aromaticivorans Hennessee et al. 2009

Synonym

Mycolicibacterium aromaticivorans

Information on morphological and physiological properties Morphology

[Ref.: #32638]	Gram stain	positive
[Ref.: #69480]	Gram stain	positive Confidence in %90.558

[Ref.: #32638]	Cell shape	rod-shaped
[Ref.: #116500]	Cell shape	rod-shaped

[Ref.: #32638]	Motility	no
[Ref.: #116500]	Motility	no

Multimedia content

[Ref.: #16676]

Caption:

Medium 250 28°C

[Ref.: #16676]

Intellectual property rights:

Information on culture and growth conditions Culture and growth conditions

[Ref.: #16676]

Culture medium

MIDDLEBROOK MEDIUM (DSMZ Medium 645)

[Ref.: #16676]

Culture medium growth

[Ref.: #16676]

Culture medium link

Medium recipe at MediaDive

[Ref.: #16676]

Culture medium composition

expand / minimize

[Ref.: #116500]

Culture medium

CIP Medium 267

[Ref.: #116500]

Culture medium growth

[Ref.: #116500]

Culture medium link

Medium recipe provided by DSMZ

		Temperature
[Ref.: #16676]	growth	28 °C
[Ref.: #32638]	growth	28-37 °C
[Ref.: #32638]	optimum	32.5 °C
[Ref.: #67770]	growth	28 °C

Information on physiology and metabolism Physiology and metabolism

[Ref.: #32638]	Oxygen tolerance	aerobe
[Ref.: #116500]	Oxygen tolerance	obligate aerobe

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #32638]		NaCl		growth	2.5-5.0	%
[Ref.: #32638]		NaCl		optimum	3.75	%

[Ref.: #32638]

Observation

aggregates in clumps

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #32638]	fructose ChEBI	+	carbon source
[Ref.: #32638]	glucose ChEBI	+	carbon source
[Ref.: #32638]	mannitol ChEBI	+	carbon source
[Ref.: #32638]	mannose ChEBI	+	carbon source
[Ref.: #116500]	nitrate ChEBI	-	reduction
[Ref.: #116500]	nitrite ChEBI	-	reduction
[Ref.: #32638]	succinate ChEBI	+	carbon source
[Ref.: #32638]	trehalose ChEBI	+	carbon source

	Metabolite production	Metabolite	Production
[Ref.: #116500]		indole ChEBI	no

	Enzyme	Enzyme activity	EC number
[Ref.: #68382]	acid phosphatase	+	3.1.3.2	* from API zym
[Ref.: #32638]	alkaline phosphatase	+	3.1.3.1
[Ref.: #68382]	alkaline phosphatase	+	3.1.3.1	* from API zym
[Ref.: #68382]	alpha-chymotrypsin	-	3.4.21.1	* from API zym
[Ref.: #68382]	alpha-fucosidase	-	3.2.1.51	* from API zym
[Ref.: #68382]	alpha-galactosidase	-	3.2.1.22	* from API zym
[Ref.: #68382]	alpha-glucosidase	+	3.2.1.20	* from API zym
[Ref.: #68382]	alpha-mannosidase	-	3.2.1.24	* from API zym
[Ref.: #68382]	beta-galactosidase	-	3.2.1.23	* from API zym
[Ref.: #68382]	beta-glucosidase	-	3.2.1.21	* from API zym
[Ref.: #68382]	beta-glucuronidase	-	3.2.1.31	* from API zym
[Ref.: #32638]	catalase	+	1.11.1.6
[Ref.: #116500]	catalase	+	1.11.1.6
[Ref.: #68382]	cystine arylamidase	+	3.4.11.3	* from API zym
[Ref.: #68382]	esterase (C 4)	+		* from API zym
[Ref.: #68382]	esterase lipase (C 8)	+		* from API zym
[Ref.: #68382]	leucine arylamidase	+	3.4.11.1	* from API zym
[Ref.: #68382]	lipase (C 14)	-		* from API zym
[Ref.: #68382]	N-acetyl-beta-glucosaminidase	-	3.2.1.52	* from API zym
[Ref.: #68382]	naphthol-AS-BI-phosphohydrolase	+		* from API zym
[Ref.: #116500]	oxidase	-
[Ref.: #68382]	trypsin	-	3.4.21.4	* from API zym
[Ref.: #116500]	urease	+	3.5.1.5
[Ref.: #68382]	valine arylamidase	-		* from API zym
	Only first 10 entries are displayed. Click here to see all.

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #116500]	11759	-	+	+	+	-	+	-	+	-	-	+	+	-	-	-	+	-	-	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #16676]	Sample type/isolated from	soil
[Ref.: #16676]	Geographic location (country and/or sea, region)	Hawai, Hilo
[Ref.: #16676]	Country	USA
[Ref.: #16676]	Country ISO 3 Code	USA
[Ref.: #16676]	Continent	North America

[Ref.: #67770]	Sample type/isolated from	Polycyclic aromatic hydrocarbon-contaminated soil of a former oil-gasification company site
[Ref.: #67770]	Geographic location (country and/or sea, region)	Hilo, HI
[Ref.: #67770]	Country	USA
[Ref.: #67770]	Country ISO 3 Code	USA
[Ref.: #67770]	Continent	North America

[Ref.: #116500]	Sample type/isolated from	Environment, Soil
[Ref.: #116500]	Geographic location (country and/or sea, region)	Hilo, Hawaii
[Ref.: #116500]	Country	United States of America
[Ref.: #116500]	Country ISO 3 Code	USA
[Ref.: #116500]	Continent	North America
* marker position based on {}

Isolation sources categories

#Environmental

#Terrestrial

#Soil

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence AY943386 (>99% sequence identity) for Mycolicibacterium from Microbeatlas

Aquatic Samples	31
Soil Samples	136
Animal Samples	53
Plant Samples	152
Total Samples	372

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #16676]	Biosafety level	1	Risk group (German classification) According to TRBA 466
[Ref.: #116500]	Biosafety level	1	Risk group (French classification)

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #16676]	Mycobacterium aromaticivorans strain JS19b1 16S ribosomal RNA gene, partial sequence	AY943386	1331	GenBank ENA	318425 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #67770]	Mycolicibacterium aromaticivorans JS19b1 = JCM 16368	GCA_000559085	contig	GenBank ENA	1440774 tax ID
[Ref.: #66792]	Mycolicibacterium aromaticivorans JS19b1 = JCM 16368	GCA_001314125	contig	GenBank ENA	1440774 tax ID	*
[Ref.: #66792]	Mycobacterium aromaticivorans JS19b1	1440774.4	wgs	PATRIC	1440774 tax ID	*
[Ref.: #66792]	Mycobacterium aromaticivorans JS19b1 = JCM 16368	1440774.6	wgs	PATRIC	1440774 tax ID	*

[Ref.: #67770]

GC-content

66.4 mol%

genome sequence analysis

Data predicted using genome information as a basis Genome-based predictions

	Trait	Model	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Mycobacterium aromaticivorans JS19b1 PATRIC: 1440774.4
[Ref.: #69480]	gram-positive	gram-positiveⓘ	yes	90.558	no
[Ref.: #69480]	anaerobic	anaerobicⓘ	no	97.636	no
[Ref.: #69480]	spore-forming	spore-formingⓘ	no	57.515	no
[Ref.: #69480]	aerobic	aerobicⓘ	yes	87.503	yes
[Ref.: #69480]	thermophilic	thermophileⓘ	no	97.5	no
[Ref.: #69480]	flagellated	motile2+ⓘ	no	87.371	no

Availability in culture collections External links

[Ref.: #16676]

Culture collection no.

DSM 45407, ATCC BAA-1378, CIP 109274, JCM 16368

[Ref.: #77957]

SI-ID 298325

Literature:

Topic	Title	Authors	Journal	DOI	Year
Genetics	Genome sequence of Mycobacterium aromaticivorans JS19b1(T), a novel isolate from Hawaiian soil.	Kwak Y, Park GS, Lee SE, Li QX, Shin JH	J Biotechnol	10.1016/j.jbiotec.2014.06.034	2014	*
Phylogeny	Polycyclic aromatic hydrocarbon-degrading species isolated from Hawaiian soils: Mycobacterium crocinum sp. nov., Mycobacterium pallens sp. nov., Mycobacterium rutilum sp. nov., Mycobacterium rufum sp. nov. and Mycobacterium aromaticivorans sp. nov.	Hennessee CT, Seo JS, Alvarez AM, Li QX	Int J Syst Evol Microbiol	10.1099/ijs.0.65827-0	2009	*

References

#16676

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 45407

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#32638

Barberan A, Caceres Velazquez H, Jones S, Fierer N.: Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information. mSphere 2: 2017 ( DOI 10.1128/mSphere.00237-17 , PubMed 28776041 ) - originally annotated from #28849 (see below)

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#68382

Automatically annotated from API zym .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#77957

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID298325.1 )

#116500 Collection of Institut Pasteur ; Curators of the CIP; CIP 109274

#28849

IJSEM 378 2008 ( DOI 10.1099/ijs.0.65827-0 , PubMed 19196782 )

* These data were automatically processed and therefore are not curated

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