Mycobacterium abscessus subsp. massiliense | Type strain | DSM 45103, CCUG 48898, CIP 108297, KCTC 19086, JCM 15300, Timone LB, BCRC 16916 | BacDiveID:8546

Strain identifier

BacDive ID: 8546

Type strain:

Species: Mycobacterium abscessus subsp. massiliense

Culture col. no.: DSM 45103, CCUG 48898, CIP 108297, KCTC 19086, JCM 15300, Timone LB, BCRC 16916

Strain history: CIP <- 2004, M. Drancourt, Timone Hosp., Marseille, France: strain LB, Mycobacterium massiliense

NCBI tax ID(s): 1962118 (subspecies)

SI-ID 134242

CCUG 48898, CIP 108297, KCTC 19086, DSM 45103, JCM 15300

Other strains from Mycobacterium abscessus subsp. massiliense

M. abscessus subsp. massiliense 19005109, DSM 113267
M. abscessus subsp. massiliense 20004644, DSM 113268

Section

Mycobacterium abscessus subsp. massiliense DSM 45103 is a microaerophile, mesophilic, Gram-positive human pathogen that was isolated from human sputum and bronchial alveolar lavage of 50 year old woman.

Gram-positive
human pathogen
mesophilic
microaerophile
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Actinomycetota*
	Class	*Actinomycetes*
	Order	*Mycobacteriales*
	Family	*Mycobacteriaceae*
	Genus	*Mycobacterium*
	Species	**Mycobacterium abscessus subsp. massiliense**
	Full Scientific Name (LPSN)	Mycobacterium abscessus subsp. massiliense (Adékambi et al. 2006) Tortoli et al. 2016

	Synonym	Mycobacterium massiliense
	Synonym	Mycobacteroides abscessus subsp. massiliense

Information on morphological and physiological properties Morphology

[Ref.: #67771]	Gram stain	positive
[Ref.: #115979]	Gram stain	positive

[Ref.: #115979]	Cell shape	rod-shaped

[Ref.: #115979]	Motility	no

[Ref.: #115979]	Hemolysis ability	1

[Ref.: #58389]	Incubation period	2-3 days

Multimedia content

Intellectual property rights:

© Leibniz-Institut DSMZ

Intellectual property rights:

© Leibniz-Institut DSMZ

Intellectual property rights:

© Leibniz-Institut DSMZ

Intellectual property rights:

© Leibniz-Institut DSMZ

Caption:

Medium 645 37°C

Intellectual property rights:

© Leibniz-Institut DSMZ

Information on culture and growth conditions Culture and growth conditions

Culture medium

MIDDLEBROOK MEDIUM (DSMZ Medium 645)

Culture medium growth

Culture medium link

Medium recipe at MediaDive

Culture medium composition

expand / minimize

[Ref.: #115979]

Culture medium

CIP Medium 45

[Ref.: #115979]

Culture medium growth

[Ref.: #115979]

Culture medium link

Medium recipe provided by DSMZ

[Ref.: #115979]

Culture medium

CIP Medium 55

[Ref.: #115979]

Culture medium growth

[Ref.: #115979]

Culture medium link

Medium recipe provided by DSMZ

	Temperatures	Kind of temperature		Temperature
[Ref.: #12345]			growth	37 °C
[Ref.: #58389]			growth	37 °C
[Ref.: #67770]			growth	37 °C
[Ref.: #67771]			growth	37 °C

[Ref.: #12345]	Temperature range	mesophilic
[Ref.: #58389]	Temperature range	mesophilic
[Ref.: #67770]	Temperature range	mesophilic
[Ref.: #67771]	Temperature range	mesophilic

Information on physiology and metabolism Physiology and metabolism

[Ref.: #58389]	Oxygen tolerance	microaerophile
[Ref.: #67771]	Oxygen tolerance	aerobe
[Ref.: #115979]	Oxygen tolerance	obligate aerobe

	Metabolite utilization	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #115979]		nitrate ChEBI	-	reduction
[Ref.: #115979]		nitrite ChEBI	-	reduction

	Metabolite production	Metabolite	Production
[Ref.: #115979]		indole ChEBI	no

	Enzymes	Enzyme	Enzyme activity	EC number
[Ref.: #68382]		acid phosphatase	+	3.1.3.2	* from API zym
[Ref.: #115979]		alcohol dehydrogenase	-	1.1.1.1
[Ref.: #68382]		alkaline phosphatase	-	3.1.3.1	* from API zym
[Ref.: #68382]		alpha-chymotrypsin	-	3.4.21.1	* from API zym
[Ref.: #68382]		alpha-fucosidase	-	3.2.1.51	* from API zym
[Ref.: #68382]		alpha-galactosidase	-	3.2.1.22	* from API zym
[Ref.: #68382]		alpha-glucosidase	+	3.2.1.20	* from API zym
[Ref.: #68382]		alpha-mannosidase	-	3.2.1.24	* from API zym
[Ref.: #68382]		beta-galactosidase	-	3.2.1.23	* from API zym
[Ref.: #68382]		beta-glucosidase	-	3.2.1.21	* from API zym
[Ref.: #68382]		beta-glucuronidase	-	3.2.1.31	* from API zym
[Ref.: #115979]		catalase	+	1.11.1.6
[Ref.: #68382]		cystine arylamidase	-	3.4.11.3	* from API zym
[Ref.: #68382]		esterase (C 4)	+		* from API zym
[Ref.: #68382]		esterase lipase (C 8)	+		* from API zym
[Ref.: #68382]		leucine arylamidase	+	3.4.11.1	* from API zym
[Ref.: #68382]		lipase (C 14)	-		* from API zym
[Ref.: #115979]		lysine decarboxylase	-	4.1.1.18
[Ref.: #68382]		N-acetyl-beta-glucosaminidase	-	3.2.1.52	* from API zym
[Ref.: #68382]		naphthol-AS-BI-phosphohydrolase	+		* from API zym
[Ref.: #115979]		ornithine decarboxylase	-	4.1.1.17
[Ref.: #115979]		oxidase	-
[Ref.: #68382]		trypsin	-	3.4.21.4	* from API zym
[Ref.: #115979]		urease	-	3.5.1.5
[Ref.: #68382]		valine arylamidase	-		* from API zym
		Only first 10 entries are displayed. Click here to see all.

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #115979]	11063	-	-	+	+	-	+	-	-	-	-	+	+	-	-	-	+	-	-	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #12345]	Sample type/isolated from	human sputum and bronchial alveolar lavage of 50 year old woman
[Ref.: #12345]	Country	France
[Ref.: #12345]	Country ISO 3 Code	FRA
[Ref.: #12345]	Continent	Europe

[Ref.: #58389]	Sample type/isolated from	Human sputum and bronchial alveolar lavage,50y.w.
[Ref.: #58389]	Geographic location (country and/or sea, region)	Marseille
[Ref.: #58389]	Country	France
[Ref.: #58389]	Country ISO 3 Code	FRA
[Ref.: #58389]	Continent	Europe

[Ref.: #67770]	Sample type/isolated from	Sputum of a patient with hemoptoic pneumonia

[Ref.: #67771]	Sample type/isolated from	From Human sputum and bronchial alveolar lavage, 50y.w.
[Ref.: #67771]	Geographic location (country and/or sea, region)	Marseille
[Ref.: #67771]	Country	France
[Ref.: #67771]	Country ISO 3 Code	FRA
[Ref.: #67771]	Continent	Europe

[Ref.: #115979]	Sample type/isolated from	Human, Sputum
[Ref.: #115979]	Geographic location (country and/or sea, region)	Marseille
[Ref.: #115979]	Country	France
[Ref.: #115979]	Country ISO 3 Code	FRA
[Ref.: #115979]	Continent	Europe
[Ref.: #115979]	Isolation date	2001
* marker position based on {}

Isolation sources categories

#Host	#Human	#Female
#Host Body Product	#Oral cavity and Airways	#Bronchial wash
#Host Body Product	#Fluids	#Sputum

What are isolation sources categories?

Global distribution of 16S sequence AY593980 (>99% sequence identity) for Mycobacteroides from Microbeatlas

Aquatic Samples	6199
Soil Samples	7046
Animal Samples	7007
Plant Samples	1742
Total Samples	21994

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #12345]	Pathogenicity (human)	yes
[Ref.: #12345]	Pathogenicity (animal)	yes
[Ref.: #12345]	Biosafety level	2	Risk group (German classification) According to TRBA 466
[Ref.: #115979]	Biosafety level	2	Risk group (French classification)

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #67770]	Mycobacterium abscessus subsp. bolletii gene for 16S ribosomal RNA, partial sequence, strain: JCM 15300	AB548602	1468	ENA	1001714 tax ID
[Ref.: #12345]	Mycobacterium abscessus subsp. bolletii strain CCUG 48898 16S ribosomal RNA gene, partial sequence	AY593980	1481	ENA	1962118 tax ID
[Ref.: #20218]	Mycobacterium massiliense 16S-23S ribosomal RNA intergenic spacer, partial sequence	AY593978	258	ENA	319705 tax ID	*
[Ref.: #20218]	Mycobacterium massiliense strain CIP 108297 16S ribosomal RNA gene, partial sequence	EU191912	457	ENA	319705 tax ID	*

Genome sequence information:

Only first 5 entries are displayed. Click here to see all.

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #67770]	Mycobacteroides abscessus subsp. massiliense CCUG 48898 = JCM 15300	GCA_000239055	contig	GenBank	1001714 tax ID
[Ref.: #67770]	Mycobacteroides abscessus subsp. massiliense CCUG 48898 = JCM 15300 CCUG48898	GCA_000270665	contig	GenBank	1001714 tax ID
[Ref.: #67770]	Mycobacteroides abscessus subsp. massiliense CCUG 48898	GCA_002086375	contig	GenBank	1962118 tax ID
[Ref.: #66792]	Mycobacterium abscessus subsp. bolletii strain CIP 108297	319705.17	wgs	PATRIC	319705 tax ID	*
[Ref.: #66792]	Mycobacterium massiliense CCUG 48898 = JCM 15300	1001714.3	wgs	PATRIC	1001714 tax ID	*
[Ref.: #66792]	Mycobacterium massiliense CCUG 48898 = JCM 15300 [PRJDB747]	1001714.4	wgs	PATRIC	1001714 tax ID	*

[Ref.: #12345]	GC-content	64.09 mol%	sequence analysis
[Ref.: #67770]	GC-content	64.1 mol%	genome sequence analysis
[Ref.: #67770]	GC-content	62-68 mol%	high performance liquid chromatography (HPLC)

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Mycobacteroides abscessus subsp. massiliense CCUG 48898 = JCM 15300 JCM 15300 NCBI: GCA_000497265.1
[Ref.: #69480]	motile	no	96.297	no
[Ref.: #69480]	flagellated	no	98.466	no
[Ref.: #69480]	gram-positive	yes	91.065	yes
[Ref.: #69480]	anaerobic	no	99.525	yes
[Ref.: #69480]	aerobic	yes	90.22	no
[Ref.: #69480]	halophile	no	94.501	no
[Ref.: #69480]	spore-forming	no	83.836	no
[Ref.: #69480]	thermophile	no	99.443	yes
[Ref.: #69480]	glucose-util	yes	82.938	no
[Ref.: #69480]	glucose-ferment	no	93.641	no
[Ref.: #69480]	motile	no	96.297	no
[Ref.: #69480]	flagellated	no	98.466	no
[Ref.: #69480]	gram-positive	yes	91.065	yes
[Ref.: #69480]	anaerobic	no	99.525	yes
[Ref.: #69480]	aerobic	yes	90.22	no
[Ref.: #69480]	halophile	no	94.501	no
[Ref.: #69480]	spore-forming	no	83.836	no
[Ref.: #69480]	thermophile	no	99.443	yes
[Ref.: #69480]	glucose-util	yes	82.938	no
[Ref.: #69480]	glucose-ferment	no	93.641	no

Availability in culture collections External links

Culture collection no.

DSM 45103, CCUG 48898, CIP 108297, KCTC 19086, JCM 15300, Timone LB, BCRC 16916

SI-ID 134242

*

Literature:

Only first 10 entries are displayed. Click here to see all.

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Emended description of Mycobacterium abscessus, Mycobacterium abscessus subsp. abscessus and Mycobacteriumabscessus subsp. bolletii and designation of Mycobacteriumabscessus subsp. massiliense comb. nov.	Tortoli E, Kohl TA, Brown-Elliott BA, Trovato A, Leao SC, Garcia MJ, Vasireddy S, Turenne CY, Griffith DE, Philley JV, Baldan R, Campana S, Cariani L, Colombo C, Taccetti G, Teri A, Niemann S, Wallace RJ Jr, Cirillo DM	Int J Syst Evol Microbiol	10.1099/ijsem.0.001376	2016	*
Genetics	Complete genome sequence and comparative genomic analysis of Mycobacterium massiliense JCM 15300 in the Mycobacterium abscessus group reveal a conserved genomic island MmGI-1 related to putative lipid metabolism.	Sekizuka T, Kai M, Nakanaga K, Nakata N, Kazumi Y, Maeda S, Makino M, Hoshino Y, Kuroda M	PLoS One	10.1371/journal.pone.0114848	2014	*
Genetics	Discrimination of Mycobacterium abscessus subsp. massiliense from Mycobacterium abscessus subsp. abscessus in clinical isolates by multiplex PCR.	Nakanaga K, Sekizuka T, Fukano H, Sakakibara Y, Takeuchi F, Wada S, Ishii N, Makino M, Kuroda M, Hoshino Y	J Clin Microbiol	10.1128/JCM.01327-13	2013	*
Genetics	Genomic insights into the emerging human pathogen Mycobacterium massiliense.	Tettelin H, Sampaio EP, Daugherty SC, Hine E, Riley DR, Sadzewicz L, Sengamalay N, Shefchek K, Su Q, Tallon LJ, Conville P, Olivier KN, Holland SM, Fraser CM, Zelazny AM	J Bacteriol	10.1128/JB.01200-12	2012	*
Transcriptome	Multilocus sequence analysis and rpoB sequencing of Mycobacterium abscessus (sensu lato) strains.	Macheras E, Roux AL, Bastian S, Leao SC, Palaci M, Sivadon-Tardy V, Gutierrez C, Richter E, Rusch-Gerdes S, Pfyffer G, Bodmer T, Cambau E, Gaillard JL, Heym B	J Clin Microbiol	10.1128/JCM.01274-10	2010	*
Phylogeny	Amoebal coculture of "Mycobacterium massiliense" sp. nov. from the sputum of a patient with hemoptoic pneumonia.	Adekambi T, Reynaud-Gaubert M, Greub G, Gevaudan MJ, La Scola B, Raoult D, Drancourt M	J Clin Microbiol	10.1128/JCM.42.12.5493-5501.2004	2004	*
Genetics	The genome sequence of 'Mycobacterium massiliense' strain CIP 108297 suggests the independent taxonomic status of the Mycobacterium abscessus complex at the subspecies level.	Cho YJ, Yi H, Chun J, Cho SN, Daley CL, Koh WJ, Shin SJ	PLoS One	10.1371/journal.pone.0081560	2013	*
	Increased virulence of an epidemic strain of Mycobacterium massiliense in mice.	Shang S, Gibbs S, Henao-Tamayo M, Shanley CA, McDonnell G, Duarte RS, Ordway DJ, Jackson M	PLoS One	10.1371/journal.pone.0024726	2011	*

References

#12345

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 45103

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#20218

Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )

#58389 Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 48898

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;
#67771 Korean Collection for Type Cultures (KCTC) ; Curators of the KCTC;

#68382

Automatically annotated from API zym .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#77930

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID134242.1 )

#115979 Collection of Institut Pasteur ; Curators of the CIP; CIP 108297
* These data were automatically processed and therefore are not curated

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