Name: Mycobacterium abscessus subsp. bolletii BD
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 8547

Type strain:

Species: Mycobacterium abscessus subsp. bolletii

Strain Designation: BD

Culture col. no.: DSM 45149, CCUG 50184, CIP 108541, JCM 15297, BCRC 16915

Strain history: CIP <- 2004, M. Drancourt, Timone Hosp., Marseille, France: strain BD

NCBI tax ID(s): 1091046 (strain), 319705 (subspecies)

SI-ID 289776

CIP 108541, CCUG 50184, JCM 15297, DSM 45149

Section

Mycobacterium abscessus subsp. bolletii BD is an aerobe, mesophilic human pathogen that was isolated from sputum.

aerobe
human pathogen
mesophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Actinomycetota*
	Class	*Actinomycetes*
	Order	*Mycobacteriales*
	Family	*Mycobacteriaceae*
	Genus	*Mycobacterium*
	Species	**Mycobacterium abscessus subsp. bolletii**
	Full Scientific Name (LPSN)	Mycobacterium abscessus subsp. bolletii (Adékambi et al. 2006) Leao et al. 2011

	Synonym	Mycobacterium bolletii
	Synonym	Mycobacteroides abscessus subsp. bolletii

Information on morphological and physiological properties Morphology

[Ref.: #115981]	Gram stain	positive
[Ref.: #69480]	Gram stain	positive Confidence in %100

[Ref.: #115981]	Cell shape	rod-shaped

[Ref.: #115981]	Motility	no
[Ref.: #69480]	Motility	no Confidence in %96.361

[Ref.: #58774]

Incubation period

2-3 days

Multimedia content

[Ref.: #15460]

Caption:

Medium 645 28°C

[Ref.: #15460]

Intellectual property rights:

Information on culture and growth conditions Culture and growth conditions

[Ref.: #42211]

Culture medium

MEDIUM 93 - for Mycobacterium avium subsp. sylvaticum

[Ref.: #42211]

Culture medium growth

[Ref.: #42211]

Culture medium composition

expand / minimize

[Ref.: #15460]

Culture medium

MIDDLEBROOK MEDIUM (DSMZ Medium 645)

[Ref.: #15460]

Culture medium growth

[Ref.: #15460]

Culture medium link

Medium recipe at MediaDive

[Ref.: #15460]

Culture medium composition

expand / minimize

[Ref.: #115981]

Culture medium

CIP Medium 93

[Ref.: #115981]

Culture medium growth

[Ref.: #115981]

Culture medium link

Medium recipe provided by DSMZ

	Kind of temperature		Temperature
[Ref.: #15460]		growth	30 °C
[Ref.: #42211]		growth	30 °C
[Ref.: #58774]		growth	37 °C
[Ref.: #67770]		growth	37 °C
[Ref.: #115981]		growth	22-37 °C
[Ref.: #115981]	no	growth	10 °C
[Ref.: #115981]	no	growth	45 °C

[Ref.: #15460]	Temperature range	mesophilic
[Ref.: #42211]	Temperature range	mesophilic
[Ref.: #58774]	Temperature range	mesophilic
[Ref.: #67770]	Temperature range	mesophilic
[Ref.: #115981]	Temperature range	psychrophilic
[Ref.: #115981]	Temperature range	thermophilic

Information on physiology and metabolism Physiology and metabolism

[Ref.: #58774]	Oxygen tolerance	aerobe
[Ref.: #115981]	Oxygen tolerance	obligate aerobe

[Ref.: #69480]

Ability of spore formation

no Confidence in %99.769

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #115981]	hippurate ChEBI	-	hydrolysis
[Ref.: #115981]	nitrate ChEBI	-	reduction
[Ref.: #115981]	nitrate ChEBI	-	respiration
[Ref.: #115981]	nitrite ChEBI	-	reduction

	Antibiotica	Metabolite	Antibiotic sensitivity	Antibiotic resistance
[Ref.: #115981]		0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)	no	yes

	Metabolite production	Metabolite	Production
[Ref.: #115981]		indole ChEBI	no

	Enzyme	Enzyme activity	EC number
[Ref.: #68382]	acid phosphatase	-	3.1.3.2	* from API zym
[Ref.: #68382]	alkaline phosphatase	-	3.1.3.1	* from API zym
[Ref.: #68382]	alpha-chymotrypsin	-	3.4.21.1	* from API zym
[Ref.: #68382]	alpha-fucosidase	-	3.2.1.51	* from API zym
[Ref.: #68382]	alpha-galactosidase	-	3.2.1.22	* from API zym
[Ref.: #68382]	alpha-glucosidase	+	3.2.1.20	* from API zym
[Ref.: #68382]	alpha-mannosidase	-	3.2.1.24	* from API zym
[Ref.: #115981]	amylase	-
[Ref.: #115981]	beta-galactosidase	-	3.2.1.23
[Ref.: #68382]	beta-galactosidase	-	3.2.1.23	* from API zym
[Ref.: #68382]	beta-glucosidase	-	3.2.1.21	* from API zym
[Ref.: #68382]	beta-glucuronidase	-	3.2.1.31	* from API zym
[Ref.: #115981]	caseinase	-	3.4.21.50
[Ref.: #115981]	catalase	+	1.11.1.6
[Ref.: #68382]	cystine arylamidase	+	3.4.11.3	* from API zym
[Ref.: #115981]	DNase	-
[Ref.: #68382]	esterase (C 4)	+		* from API zym
[Ref.: #68382]	esterase lipase (C 8)	+		* from API zym
[Ref.: #115981]	gamma-glutamyltransferase	+	2.3.2.2
[Ref.: #115981]	gelatinase	-
[Ref.: #115981]	lecithinase	-
[Ref.: #68382]	leucine arylamidase	+	3.4.11.1	* from API zym
[Ref.: #115981]	lipase	-
[Ref.: #68382]	lipase (C 14)	-		* from API zym
[Ref.: #68382]	N-acetyl-beta-glucosaminidase	-	3.2.1.52	* from API zym
[Ref.: #68382]	naphthol-AS-BI-phosphohydrolase	-		* from API zym
[Ref.: #115981]	oxidase	-
[Ref.: #115981]	protease	-
[Ref.: #68382]	trypsin	-	3.4.21.4	* from API zym
[Ref.: #115981]	tween esterase	-
[Ref.: #115981]	urease	-	3.5.1.5
[Ref.: #68382]	valine arylamidase	-		* from API zym
	Only first 10 entries are displayed. Click here to see all.

API® Biotype100:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	ControlQ	D-glucose as carbon sourceGLU	D-fructose as carbon sourceFRU	D-galactose as carbon sourceGAL	D-trehalose as carbon sourceTRE	D-mannose as carbon sourceMNE	L-sorbose as carbon sourceSBE	D-melibiose as carbon sourceMEL	sucrose as carbon sourceSAC	D-raffinose as carbon sourceRAF	maltotriose as carbon sourceMTE	maltose as carbon sourceMAL	lactose as carbon sourceLAC	lactulose as carbon sourceLTE	1-O-methyl-beta-galactopyranoside as carbon sourceMbGa	1-O-methyl-alpha-galactopyranoside as carbon sourceMaGa	D-cellobiose as carbon sourceCEL	gentiobiose as carbon sourceGEN	1-O-methyl-beta-D-glucopyranoside as carbon sourceMbGu	esculin as carbon sourceESC	D-ribose as carbon sourceRIB	L-arabinose as carbon sourceARA	D-xylose as carbon sourceXYL	palatinose as carbon sourcePLE	L-rhamnose as carbon sourceRHA	L-fucose as carbon sourceFUC	D-melezitose as carbon sourceMLZ	D-arabitol as carbon sourceDARL	L-arabitol as carbon sourceLARL	xylitol as carbon sourceXLT	dulcitol as carbon sourceDUL	D-tagatose as carbon sourceTAG	glycerol as carbon sourceGLY	myo-inositol as carbon sourceINO	D-mannitol as carbon sourceMAN	maltitol as carbon sourceMTL	D-turanose as carbon sourceTUR	D-sorbitol as carbon sourceSOR	adonitol as carbon sourceADO	hydroxyquinoline-beta-glucuronide as carbon sourceHBG	D-lyxose as carbon sourceLYX	i-erythritol as carbon sourceERY	1-O-methyl-alpha-D-glucoside as carbon sourceMDG	3-O-methyl-D-glucose as carbon source3MDG	D-saccharate as carbon sourceSAT	mucate as carbon sourceMUC	L-tartrate as carbon sourceLTAT	D-tartrate as carbon sourceDTAT	meso-tartrate as carbon sourceMTAT	D-malate as carbon sourceDMLT	L-malate as carbon sourceLMLT	cis-aconitate as carbon sourceCATE	trans-aconitate as carbon sourceTATE	tricarballylate as carbon sourceTTE	citrate as carbon sourceCIT	D-glucuronate as carbon sourceGRT	D-galacturonate as carbon sourceGAT	2-ketogluconate as carbon source2KG	5-ketogluconate as carbon source5KG	tryptophan as carbon sourceTRY	N-acetyl-D-glucosamine as carbon sourceNAG	D-gluconate as carbon sourceGNT	phenylacetate as carbon sourcePAC	protocatechuate as carbon sourcePAT	4-hydroxybenzoate as carbon sourcepOBE	quinate as carbon sourceQAT	gentisate as carbon sourceGTE	3-hydroxybenzoate as carbon sourcemOBE	benzoate as carbon sourceBAT	3-phenylpropionate as carbon sourcePPAT	m-coumarate as carbon sourceCMT	trigonelline as carbon sourceTGE	betaine as carbon sourceBET	putrescine as carbon sourcePCE	4-aminobutyrate as carbon sourceABT	histamine as carbon sourceHIN	DL-lactate as carbon sourceLAT	caprate as carbon sourceCAP	caprylate as carbon sourceCYT	L-histidine as carbon sourceHIS	succinate as carbon sourceSUC	fumarate as carbon sourceFUM	glutarate as carbon sourceGRE	DL-glycerate as carbon sourceGYT	5-aminovalerate as carbon sourceAVT	ethanolamine as carbon sourceETN	tryptamine as carbon sourceTTN	D-glucosamine as carbon sourceGLN	itaconate as carbon sourceITA	3-hydroxybutyrate as carbon source3OBU	L-aspartate as carbon sourceAPT	L-glutamate as carbon sourceGTT	L-proline as carbon sourcePRO	D-alanine as carbon sourceDALA	L-alanine as carbon sourceLALA	L-serine as carbon sourceSER	malonate as carbon sourceMNT	propionate as carbon sourcePROP	L-tyrosine as carbon sourceTYR	2-ketoglutarate as carbon source2KT
[Ref.: #115981]	2794	not determinedn.d.	+	-	-	+	+	-	-	-	-	-	-	-	-	-	-	-	-	-	+	-	-	-	-	-	-	-	-	-	-	-	-	+	-	-	+	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	+	+	+	-	-	-	+	+	-	-	-	-	-	-	-	-	-	-	-	+	-	-	-	-	+	-	+	+	-	-	-	+	-	-	-	+	-	+	-	-	+	+	-	+	-	-

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #115981]	11268	-	-	+	+	-	+	-	+	-	-	-	-	-	-	-	+	-	-	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #15460]	Sample type/isolated from	sputum
[Ref.: #15460]	Geographic location (country and/or sea, region)	Marseille
[Ref.: #15460]	Country	France
[Ref.: #15460]	Country ISO 3 Code	FRA
[Ref.: #15460]	Continent	Europe
[Ref.: #15460]	Geographic location	43.2965°/5.3698°

[Ref.: #58774]	Sample type/isolated from	Human bronchial lavage
[Ref.: #58774]	Geographic location (country and/or sea, region)	Marseille
[Ref.: #58774]	Country	France
[Ref.: #58774]	Country ISO 3 Code	FRA
[Ref.: #58774]	Continent	Europe

[Ref.: #67770]	Sample type/isolated from	Sputum

[Ref.: #115981]	Sample type/isolated from	Broncho-alveolar liquid
[Ref.: #115981]	Geographic location (country and/or sea, region)	Marseille
[Ref.: #115981]	Country	France
[Ref.: #115981]	Country ISO 3 Code	FRA
[Ref.: #115981]	Continent	Europe
[Ref.: #115981]	Isolation date	2001
* marker position based on {}

Isolation sources categories

#Host Body-Site

#Oral cavity and airways

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence AY859681 (>99% sequence identity) for Mycobacteroides from Microbeatlas

Aquatic Samples	6199
Soil Samples	7046
Animal Samples	7007
Plant Samples	1742
Total Samples	21994

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #15460]	Pathogenicity (human)	yes
[Ref.: #15460]	Pathogenicity (animal)	yes
[Ref.: #15460]	Biosafety level	2	Risk group (German classification) According to TRBA 466
[Ref.: #115981]	Biosafety level	2	Risk group (French classification)

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #15460]	Mycobacteroides abscessus subsp. bolletii strain CIP 108541 16S ribosomal RNA gene, partial sequence	AY859681	1481	ENA	319705 tax ID
[Ref.: #20218]	Mycobacterium bolletii strain CCUG 50184 16S-23S ribosomal RNA intergenic spacer, partial sequence; and 23S ribosomal RNA gene, complete sequence	GU143888	3401	ENA	319705 tax ID	*
[Ref.: #20218]	Mycobacterium bolletii gene for 16S ribosomal RNA, partial sequence	AB548606	1468	ENA	319705 tax ID	*
[Ref.: #20218]	Mycobacterium bolletii DNA, 16S-23S ribosomal RNA intergenic spacer, partial sequence, strain: JCM 15297	AB548607	297	ENA	319705 tax ID	*
[Ref.: #20218]	Mycobacterium bolletii strain CCUG 50184 16S ribosomal RNA gene, partial sequence	DQ987740	529	ENA	319705 tax ID	*

Genome sequence information:

Only first 5 entries are displayed. Click here to see all.

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #67770]	Mycobacteroides abscessus subsp. bolletii CIP 108541	GCA_001792615	contig	GenBank	319705 tax ID
[Ref.: #67770]	Mycobacteroides abscessus subsp. bolletii CCUG 50184	GCA_006409125	contig	GenBank	319705 tax ID
[Ref.: #67770]	Mycobacteroides abscessus subsp. bolletii BD	GCA_002013835	contig	GenBank	319705 tax ID
[Ref.: #67770]	Mycobacteroides abscessus subsp. bolletii CIP108541	GCA_002086525	contig	GenBank	319705 tax ID
[Ref.: #67770]	Mycobacteroides abscessus subsp. bolletii BD	GCA_000239035	contig	GenBank	1091046 tax ID
[Ref.: #66792]	Mycobacterium abscessus subsp. bolletii strain CIP 108541	319705.16	wgs	PATRIC	319705 tax ID	*

[Ref.: #15460]	GC-content	64.07 mol%	sequence analysis
[Ref.: #67770]	GC-content	64 mol%	genome sequence analysis
[Ref.: #67770]	GC-content	61-65 mol%	high performance liquid chromatography (HPLC)
[Ref.: #67770]	GC-content	64.1 mol%	genome sequence analysis

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Mycobacteroides abscessus subsp. bolletii BD PATRIC: 1091046.5
[Ref.: #69481]	spore-forming	no	82	no

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Mycobacteroides abscessus subsp. bolletii BD PATRIC: 1091046.5
[Ref.: #69480]	motile	no	95.692	no
[Ref.: #69480]	flagellated	no	98.332	no
[Ref.: #69480]	gram-positive	yes	89.986	no
[Ref.: #69480]	anaerobic	no	99.586	yes
[Ref.: #69480]	aerobic	yes	89.505	yes
[Ref.: #69480]	halophile	no	93.896	no
[Ref.: #69480]	spore-forming	no	79.236	no
[Ref.: #69480]	glucose-util	yes	81.213	no
[Ref.: #69480]	thermophile	no	99.446	no
[Ref.: #69480]	glucose-ferment	no	93.798	no

Availability in culture collections External links

[Ref.: #15460]

Culture collection no.

DSM 45149, CCUG 50184, CIP 108541, JCM 15297, BCRC 16915

[Ref.: #77931]

SI-ID 289776

Literature:

Topic	Title	Authors	Journal	DOI	Year
Transcriptome	Multilocus sequence analysis and rpoB sequencing of Mycobacterium abscessus (sensu lato) strains.	Macheras E, Roux AL, Bastian S, Leao SC, Palaci M, Sivadon-Tardy V, Gutierrez C, Richter E, Rusch-Gerdes S, Pfyffer G, Bodmer T, Cambau E, Gaillard JL, Heym B	J Clin Microbiol	10.1128/JCM.01274-10	2010	*
Phylogeny	rpoB gene sequence-based characterization of emerging non-tuberculous mycobacteria with descriptions of Mycobacterium bolletii sp. nov., Mycobacterium phocaicum sp. nov. and Mycobacterium aubagnense sp. nov.	Adekambi T, Berger P, Raoult D, Drancourt M	Int J Syst Evol Microbiol	10.1099/ijs.0.63969-0	2006	*

References

#15460

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 45149

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#20218

Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )

#42211 ; Curators of the CIP;
#58774 Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 50184

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#68382

Automatically annotated from API zym .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#77931

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID289776.1 )

#115981 Collection of Institut Pasteur ; Curators of the CIP; CIP 108541
* These data were automatically processed and therefore are not curated

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Mycobacteroides abscessus subsp. bolletii BD

Mycobacteroides abscessus subsp. bolletii BD

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