Mycobacterium pseudoshottsii L15 is an obligate aerobe, ovoid-shaped animal pathogen that was isolated from granulomatous lesions in splenic tissue from a striped bass.
ovoid-shaped obligate aerobe animal pathogen genome sequence 16S sequence Bacteria| @ref 20215 |
|
|
| Domain Bacteria |
| Phylum Actinomycetota |
| Class Actinomycetes |
| Order Mycobacteriales |
| Family Mycobacteriaceae |
| Genus Mycobacterium |
| Species Mycobacterium pseudoshottsii |
| Full scientific name Mycobacterium pseudoshottsii Rhodes et al. 2005 |
| 31337 | Productionyes |
| @ref: | 12350 |
| multimedia content: | DSM_45108.jpg |
| multimedia.multimedia content: | https://www.dsmz.de/microorganisms/photos/DSM_45108.jpg |
| caption: | Medium 645 25°C |
| intellectual property rights: | © Leibniz-Institut DSMZ |
| manual_annotation: | 1 |
| @ref | Name | Growth | Medium link | Composition | |
|---|---|---|---|---|---|
| 12350 | MIDDLEBROOK MEDIUM (DSMZ Medium 645) | Medium recipe at MediaDive | Name: MIDDLEBROOK MEDIUM (DSMZ Medium 645) Composition: Bacto Middlebrook 7H10 agar 20.9945 g/l Glycerol Distilled water | ||
| 37835 | MEDIUM 55 - for Mycobacterium | ||||
| 122001 | CIP Medium 93 | Medium recipe at CIP | |||
| 122001 | CIP Medium 55 | Medium recipe at CIP |
| 31337 | Spore formationno |
| @ref | Salt | Growth | Tested relation | Concentration | |
|---|---|---|---|---|---|
| 31337 | NaCl | positive | growth | 5 % |
| @ref | Value | Activity | Ec | |
|---|---|---|---|---|
| 31337 | acid phosphatase | + | 3.1.3.2 | |
| 68382 | acid phosphatase | + | 3.1.3.2 | from API zym |
| 68382 | alkaline phosphatase | + | 3.1.3.1 | from API zym |
| 68382 | alpha-chymotrypsin | - | 3.4.21.1 | from API zym |
| 68382 | alpha-fucosidase | - | 3.2.1.51 | from API zym |
| 68382 | alpha-galactosidase | - | 3.2.1.22 | from API zym |
| 68382 | alpha-glucosidase | - | 3.2.1.20 | from API zym |
| 68382 | alpha-mannosidase | - | 3.2.1.24 | from API zym |
| 68382 | beta-galactosidase | - | 3.2.1.23 | from API zym |
| 68382 | beta-glucosidase | - | 3.2.1.21 | from API zym |
| 68382 | beta-glucuronidase | - | 3.2.1.31 | from API zym |
| 31337 | catalase | + | 1.11.1.6 | |
| 122001 | catalase | + | 1.11.1.6 | |
| 68382 | cystine arylamidase | + | 3.4.11.3 | from API zym |
| 68382 | esterase (C 4) | + | from API zym | |
| 68382 | esterase lipase (C 8) | + | from API zym | |
| 68382 | leucine arylamidase | + | 3.4.11.1 | from API zym |
| 68382 | lipase (C 14) | + | from API zym | |
| 68382 | N-acetyl-beta-glucosaminidase | - | 3.2.1.52 | from API zym |
| 68382 | naphthol-AS-BI-phosphohydrolase | + | from API zym | |
| 122001 | oxidase | - | ||
| 68382 | trypsin | + | 3.4.21.4 | from API zym |
| 31337 | urease | + | 3.5.1.5 | |
| 122001 | urease | - | 3.5.1.5 | |
| 68382 | valine arylamidase | + | from API zym |
| Cat1 | Cat2 | Cat3 | |
|---|---|---|---|
| #Host | #Fishes | - | |
| #Host Body-Site | #Other | #Wound | |
| #Host Body-Site | #Organ | #Spleen |
| @ref | Sample type | Host species | Geographic location | Country | Country ISO 3 Code | Continent | Isolation date | |
|---|---|---|---|---|---|---|---|---|
| 12350 | granulomatous lesions in splenic tissue from a striped bass(Morone saxatilis) | |||||||
| 67770 | Granulomatous lesions in splenic tissue from a striped bass (Morone saxatilis) | Morone saxatilis | Chesapeake Bay | USA | USA | North America | ||
| 122001 | Granulomatous splenix lesion in striped bass, Morone saxatilis | Chesapeake bay | United States of America | USA | North America | 2002 |
Global distribution of 16S sequence AY570988 (>99% sequence identity) for Mycobacterium from Microbeatlas ![]()
| @ref | Description | Assembly level | INSDC accession | BV-BRC accession | IMG accession | NCBI tax ID | Score | |
|---|---|---|---|---|---|---|---|---|
| 66792 | ASM358474v1 assembly for Mycobacterium pseudoshottsii JCM 15466 | complete | 1136880 | 95.74 | ||||
| 67770 | ASM151536v1 assembly for Mycobacterium pseudoshottsii JCM 15466 | scaffold | 1136880 | 10.07 | ||||
| 66792 | ASM2582150v1 assembly for Mycobacterium pseudoshottsii DSM 45108 | contig | 265949 |
| @ref | Description | Accession | Length | Database | NCBI tax ID | |
|---|---|---|---|---|---|---|
| 20218 | Mycobacterium pseudoshottsii partial 16S rRNA gene, type strain NCTC 13318T | AM884320 | 565 | 1136880 | ||
| 20218 | Mycobacterium pseudoshottsii strain NCTC 13318 16S ribosomal RNA gene, partial sequence | DQ987742 | 543 | 1136880 | ||
| 12350 | Mycobacterium pseudoshottsii 16S ribosomal RNA gene, partial sequence | AY570988 | 1453 | 1136880 | ||
| 67770 | Mycobacterium pseudoshottsii gene for 16S ribosomal RNA, partial sequence, strain: JCM 15466 | AB548713 | 1475 | 1136880 | ||
| 124043 | Mycobacterium pseudoshottsii DNA, 16S-23S ribosomal RNA intergenic spacer, partial sequence, strain: JCM 15466. | AB548714 | 272 | 1136880 |
| @ref | GC-content (mol%) | Method | |
|---|---|---|---|
| 67770 | 65.7 | genome sequence analysis |
| @ref | Trait | Model | Prediction | Confidence in % | In training data |
|---|---|---|---|---|---|
| 125439 | oxygen_tolerance | BacteriaNetⓘ | obligate aerobe | 99.20 | no |
| 125439 | gram_stain | BacteriaNetⓘ | positive | 97.70 | no |
| 125439 | motility | BacteriaNetⓘ | no | 93.60 | no |
| 125439 | spore_formation | BacteriaNetⓘ | yes | 73.60 | no |
| @ref | Trait | Model | Prediction | Confidence in % | In training data |
|---|---|---|---|---|---|
| 125438 | gram-positive | gram-positiveⓘ | yes | 88.92 | no |
| 125438 | anaerobic | anaerobicⓘ | no | 98.04 | yes |
| 125438 | aerobic | aerobicⓘ | yes | 80.66 | yes |
| 125438 | spore-forming | spore-formingⓘ | no | 64.63 | yes |
| 125438 | thermophilic | thermophileⓘ | no | 95.40 | yes |
| 125438 | flagellated | motile2+ⓘ | no | 92.00 | no |
| Topic | Title | Authors | Journal | DOI | Year | |
|---|---|---|---|---|---|---|
| Phylogeny | Application of Genomic Epidemiology of Pathogens to Farmed Yellowtail Fish Mycobacteriosis in Kyushu, Japan. | Wada T, Yoshida S, Yamamoto T, Nonaka L, Fukushima Y, Nakajima C, Suzuki Y, Imajoh M. | Microbes Environ | 10.1264/jsme2.me24011 | 2024 | |
| Retrospective identification of pathogenic mycobacterial species in fish: Mycobacterium pseudoshottsii YM-3, isolated from a yellowtail fish in 1986 in Kochi, Japan. | Imajoh M, Yoshida S, Nonaka L, Fukushima Y, Nakajima C, Suzuki Y, Wada T. | Microbiol Resour Announc | 10.1128/mra.00498-23 | 2023 | ||
| Phylogeny | Buruli ulcer caused by Mycobacterium ulcerans subsp. shinshuense: A case report. | Fujimori T, Hagiya H, Iio K, Yamasaki O, Miyamoto Y, Hoshino Y, Kakehi A, Okura M, Minabe H, Yokoyama Y, Otsuka F, Higashikage A. | J Infect Chemother | 10.1016/j.jiac.2023.02.009 | 2023 | |
| Identification of Mycobacterium pseudoshottsii in the Eastern Mediterranean. | Davidovich N, Makhon A, Zizelski Valenci G, Dveyrin Z, Yahav T, Pretto T, Tchernov D, Morick D. | Microbiol Spectr | 10.1128/spectrum.00856-23 | 2023 | ||
| Complete Genome and Partial Megaplasmid Sequences of Mycobacterium pseudoshottsii Strain NJB1907-Z4, Isolated from an Aquarium-Reared Japanese Sardine (Sardinops melanostictus) in Japan. | Komine T, Fukano H, Yoshida M, Inohana M, Hoshino Y, Kurata O, Wada S. | Microbiol Resour Announc | 10.1128/mra.00785-22 | 2022 | ||
| Elevated temperature inhibits Mycobacterium shottsii infection and Mycobacterium pseudoshottsii disease in striped bass Morone saxatilis. | Gauthier DT, Haines AN, Vogelbein WK. | Dis Aquat Organ | 10.3354/dao03584 | 2021 | ||
| A case of mycobacteriosis associated with Mycobacterium pseudoshottsii in aquarium-reared fish in Japan. | Komine T, Ihara H, Ono K, Yoshida M, Sugimoto Y, Inohana M, Fukano H, Kurata O, Wada S. | J Vet Med Sci | 10.1292/jvms.22-0318 | 2022 | ||
| A rapid and simple MALDI-TOF MS lipid profiling method for differentiating Mycobacterium ulcerans from Mycobacterium marinum. | Komine T, Fukano H, Yoshida M, Miyamoto Y, Nakaya M, Fujinaga A, Doke K, Hoshino Y. | J Clin Microbiol | 10.1128/jcm.01400-24 | 2025 | ||
| Genomic Insights into the Fish-Pathogenic Mycobacterium pseudoshottsii Strain AR Recovered from Meagre (Argyrosomus regius). | Stathopoulou P, Asimakis E, Petropoulos Y, Apostolopoulou G, Tsiamis G. | Microbiol Resour Announc | 10.1128/mra.01099-20 | 2020 | ||
| Availability of culture filtrate protein-10 (CFP-10) secreted from Mycobacterium pseudoshottsii for mycobacteriosis diagnosis in ginbuna crucian carp Carrasius auratus langsdorfii. | Matsumoto M, Hayashi K, Araki K, Nakanishi T, Yamamoto A. | J Fish Dis | 10.1111/jfd.13108 | 2020 | ||
| One-step loop-mediated isothermal amplification system for Mycobacterium marinum detection. | Okumura K, Miyamoto Y, Mitarai S, Ato M. | Microbiol Spectr | 10.1128/spectrum.02906-24 | 2025 | ||
| Mycobacterium pseudoshottsii in Mediterranean Fish Farms: New Trouble for European Aquaculture? | Mugetti D, Varello K, Gustinelli A, Pastorino P, Menconi V, Florio D, Fioravanti ML, Bozzetta E, Zoppi S, Dondo A, Prearo M. | Pathogens | 10.3390/pathogens9080610 | 2020 | ||
| Genetics | Genomic Degeneration and Reduction in the Fish Pathogen Mycobacterium shottsii. | Gauthier DT, Doss JH, LaGatta M, Gupta T, Karls RK, Quinn FD. | Microbiol Spectr | 10.1128/spectrum.01158-21 | 2022 | |
| Density Functional Theory-Based Studies Predict Carbon Nanotubes as Effective Mycolactone Inhibitors. | Suleiman N, Yaya A, Wilson MD, Aryee S, Kwofie SK. | Molecules | 10.3390/molecules27144440 | 2022 | ||
| Mycobacterium marinum: A Case-Based Narrative Review of Diagnosis and Management. | Tsiolakkis G, Liontos A, Filippas-Ntekouan S, Matzaras R, Theodorou E, Vardas M, Vairaktari G, Nikopoulou A, Christaki E. | Microorganisms | 10.3390/microorganisms11071799 | 2023 | ||
| Internalization of Mycobacterium shottsii and Mycobacterium pseudoshottsii by Acanthamoeba polyphaga. | Gupta T, Fine-Coulson K, Karls R, Gauthier D, Quinn F. | Can J Microbiol | 10.1139/cjm-2013-0079 | 2013 | ||
| High female mortality caused by an atypical Mycobacterium species closely related to the Mycobacterium ulcerans-marinum complex in a colony of bearded dragons (Pogona vitticeps). | Chenier S, Tremblay M, Lloyd D, Duceppe MO, Andrievskaia O. | Can Vet J | 2022 | |||
| Enzymology | Mycobacterium pseudoshottsii isolated from 24 farmed fishes in western Japan. | Nakanaga K, Hoshino Y, Hattori Y, Yamamoto A, Wada S, Hatai K, Makino M, Ishii N. | J Vet Med Sci | 10.1292/jvms.11-0226 | 2012 | |
| Pathogenicity | Systematic review: Global host range, case fatality and detection rates of Mycobacterium ulcerans in humans and potential environmental sources. | Tchatchouang S, Andre Mbongue Mikangue C, Kenmoe S, Bowo-Ngandji A, Mahamat G, Thierry Ebogo-Belobo J, Serge Mbaga D, Rodrigue Foe-Essomba J, Numfor H, Irma Kame-Ngasse G, Nyebe I, Bosco Taya-Fokou J, Zemnou-Tepap C, Felicite Yengue J, Nina Magoudjou-Pekam J, Gertrude Djukouo L, Antoinette Kenmegne Noumbissi M, Kenfack-Momo R, Aimee Touangnou-Chamda S, Flore Feudjio A, Gael Oyono M, Paola Demeni Emoh C, Raoul Tazokong H, Zeukeng F, Kengne-Nde C, Njouom R, Flore Donkeng Donfack V, Eyangoh S. | J Clin Tuberc Other Mycobact Dis | 10.1016/j.jctube.2024.100457 | 2024 | |
| Enzymology | A sensitive FRET probe assay for the selective detection of Mycobacterium marinum in fish. | Salati F, Meloni M, Fenza A, Angelucci G, Colorni A, Orru G. | J Fish Dis | 10.1111/j.1365-2761.2009.01112.x | 2010 | |
| Phylogeny | Expanded range and new host species of Mycobacterium shottsii and M. pseudoshottsii. | Stine CB, Jacobs JM, Rhodes MR, Overton A, Fast M, Baya AM. | J Aquat Anim Health | 10.1577/h09-005.1 | 2009 | |
| Metabolism | Mycobacterium ulcerans mycolactones-fungi crosstalking. | Hammoudi N, Cassagne C, Armstrong N, Ranque S, Henrissat B, Drancourt M, Bouam A. | Sci Rep | 10.1038/s41598-019-39927-3 | 2019 | |
| Genetics | Whole-Genome sequencing and comparative genomics of Mycobacterium spp. from farmed Atlantic and coho salmon in Chile. | Suarez R, Kusch K, Miranda CD, Li T, Campanini J, Behra PRK, Aro L, Martinez A, Godoy M, Medina DA. | Antonie Van Leeuwenhoek | 10.1007/s10482-021-01592-w | 2021 | |
| Phylogeny | Non-tuberculous Mycobacterium species causing mycobacteriosis in farmed aquatic animals of South Africa. | Gcebe N, Michel AL, Hlokwe TM. | BMC Microbiol | 10.1186/s12866-018-1177-9 | 2018 | |
| Genetics | Variable Number Tandem Repeat Profiling of Mycobacterium ulcerans Reveals New Genotypes in Buruli Ulcer Endemic Communities in Ghana and Côte d'Ivoire. | Gyamfi E, Dogbe MA, Quaye C, Affouda AA, Kyei-Baffour E, Awuku-Asante D, Sarpong-Duah M, Mosi L. | Front Microbiol | 10.3389/fmicb.2022.872579 | 2022 | |
| Detection of Mycobacterium ulcerans DNA in the Environment, Ivory Coast. | Tian RB, Niamke S, Tissot-Dupont H, Drancourt M. | PLoS One | 10.1371/journal.pone.0151567 | 2016 | ||
| Quantitative PCR assay for Mycobacterium pseudoshottsii and Mycobacterium shottsii and application to environmental samples and fishes from the Chesapeake Bay. | Gauthier DT, Reece KS, Xiao J, Rhodes MW, Kator HI, Latour RJ, Bonzek CF, Hoenig JM, Vogelbein WK. | Appl Environ Microbiol | 10.1128/aem.01091-10 | 2010 | ||
| Enzymology | Nineteen cases of Buruli ulcer diagnosed in Japan from 1980 to 2010. | Nakanaga K, Hoshino Y, Yotsu RR, Makino M, Ishii N. | J Clin Microbiol | 10.1128/jcm.00783-11 | 2011 | |
| Naturally occurring a loss of a giant plasmid from Mycobacterium ulcerans subsp. shinshuense makes it non-pathogenic. | Nakanaga K, Ogura Y, Toyoda A, Yoshida M, Fukano H, Fujiwara N, Miyamoto Y, Nakata N, Kazumi Y, Maeda S, Ooka T, Goto M, Tanigawa K, Mitarai S, Suzuki K, Ishii N, Ato M, Hayashi T, Hoshino Y. | Sci Rep | 10.1038/s41598-018-26425-1 | 2018 | ||
| Enzymology | Molecular detection of Mycobacterium ulcerans in the environment and its relationship with Buruli ulcer occurrence in Zio and Yoto districts of maritime region in Togo. | Maman I, Tchacondo T, Kere AB, Beissner M, Badziklou K, Tedihou E, Nyaku E, Amekuse K, Wiedemann FX, Karou DS, Bretzel G. | PLoS Negl Trop Dis | 10.1371/journal.pntd.0006455 | 2018 | |
| Genetics | BiG-SLiCE: A highly scalable tool maps the diversity of 1.2 million biosynthetic gene clusters. | Kautsar SA, van der Hooft JJJ, de Ridder D, Medema MH. | Gigascience | 10.1093/gigascience/giaa154 | 2021 | |
| Metabolism | Globally distributed mycobacterial fish pathogens produce a novel plasmid-encoded toxic macrolide, mycolactone F. | Ranger BS, Mahrous EA, Mosi L, Adusumilli S, Lee RE, Colorni A, Rhodes M, Small PL. | Infect Immun | 10.1128/iai.00970-06 | 2006 | |
| Buruli Ulcer, a Prototype for Ecosystem-Related Infection, Caused by Mycobacterium ulcerans. | Zingue D, Bouam A, Tian RBD, Drancourt M. | Clin Microbiol Rev | 10.1128/cmr.00045-17 | 2018 | ||
| Metabolism | Deciphering the genetic basis for polyketide variation among mycobacteria producing mycolactones. | Pidot SJ, Hong H, Seemann T, Porter JL, Yip MJ, Men A, Johnson M, Wilson P, Davies JK, Leadlay PF, Stinear TP. | BMC Genomics | 10.1186/1471-2164-9-462 | 2008 | |
| Genetics | Genome-Based Taxonomic Classification of the Phylum Actinobacteria. | Nouioui I, Carro L, Garcia-Lopez M, Meier-Kolthoff JP, Woyke T, Kyrpides NC, Pukall R, Klenk HP, Goodfellow M, Goker M. | Front Microbiol | 10.3389/fmicb.2018.02007 | 2018 | |
| Draft Genome Sequences of 25 Mycobacterium marinum Strains Isolated from Animals and Environmental Components in Aquaria and an Aquaculture Farm. | Komine T, Fukano H, Inohana M, Hoshino Y, Kurata O, Wada S. | Microbiol Resour Announc | 10.1128/mra.00851-22 | 2022 | ||
| Enzymology | First report of disseminated Mycobacterium skin infections in two liver transplant recipients and rapid diagnosis by hsp65 gene sequencing. | Lau SK, Curreem SO, Ngan AH, Yeung CK, Yuen KY, Woo PC. | J Clin Microbiol | 10.1128/jcm.05088-11 | 2011 | |
| Complete Chromosome Sequence of a Mycolactone-Producing Mycobacterium, Mycobacterium pseudoshottsii. | Yoshida M, Miyamoto Y, Ogura Y, Hayashi T, Hoshino Y | Genome Announc | 10.1128/genomeA.01363-17 | 2017 | ||
| Genetics | Draft Genome Sequence of the Fish Pathogen Mycobacterium pseudoshottsii Strain JCM15466, a Species Closely Related to M. marinum. | Hikima J, Sakai M, Aoki T, Takeyama H, Hawke J, Mori K, Tashiro K, Kuhara S | Genome Announc | 10.1128/genomeA.01630-15 | 2016 | |
| Phylogeny | Mycobacterium pseudoshottsii sp. nov., a slowly growing chromogenic species isolated from Chesapeake Bay striped bass (Morone saxatilis). | Rhodes MW, Kator H, McNabb A, Deshayes C, Reyrat JM, Brown-Elliott BA, Wallace R, Trott KA, Parker JM, Lifland B, Osterhout G, Kaattari I, Reece K, Vogelbein W, Ottinger CA | Int J Syst Evol Microbiol | 10.1099/ijs.0.63343-0 | 2005 |
| #12350 | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 45108 |
| #20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 ) |
| #20218 | Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 ) |
| #27651 | IJSEM 1139 2005 ( DOI 10.1099/ijs.0.63343-0 , PubMed 15879246 ) |
| #31337 | Barberan A, Caceres Velazquez H, Jones S, Fierer N.: Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information. mSphere 2: 2017 ( DOI 10.1128/mSphere.00237-17 , PubMed 28776041 ) - originally annotated from #27651 |
| #37835 | ; Curators of the CIP; |
| #66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) . |
| #67770 | Japan Collection of Microorganism (JCM) ; Curators of the JCM; |
| #68382 | Automatically annotated from API zym . |
| #69479 | João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta . |
| #122001 | Collection of Institut Pasteur ; Curators of the CIP; CIP 109775 |
| #124043 | Isabel Schober, Julia Koblitz: Data extracted from sequence databases, automatically matched based on designation and taxonomy . |
| #125438 | Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 ) |
| #125439 | Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 . |
| #126262 | A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 ) |
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