Mycobacterium porcinum E10241-1 is an aerobe, rod-shaped human pathogen that was isolated from swine lymph node.
rod-shaped aerobe human pathogen genome sequence 16S sequence Bacteria| @ref 20215 |
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| Domain Bacteria |
| Phylum Actinomycetota |
| Class Actinomycetes |
| Order Mycobacteriales |
| Family Mycobacteriaceae |
| Genus Mycobacterium |
| Species Mycobacterium porcinum |
| Full scientific name Mycobacterium porcinum Tsukamura et al. 1983 |
| Synonyms (1) |
| BacDive ID | Other strains from Mycobacterium porcinum (1) | Type strain |
|---|---|---|
| 8472 | M. porcinum MF-155, W6238, CCUG 47583, CIP 107826, DSM ... |
| @ref | Name | Growth | Medium link | Composition | |
|---|---|---|---|---|---|
| 11617 | MYCOBACTERIUM MEDIUM (DSMZ Medium 219) | Medium recipe at MediaDive | Name: MYCOBACTERIUM MEDIUM (DSMZ Medium 219) Composition: Agar 20.0 g/l Na2HPO4 x 12 H2O 2.5 g/l Yeast extract 2.0 g/l Proteose peptone no. 3 2.0 g/l Casein peptone 2.0 g/l Sodium citrate 1.5 g/l KH2PO4 1.0 g/l MgSO4 x 7 H2O 0.6 g/l Tween 0.5 g/l Glycerol Distilled water | ||
| 11617 | MIDDLEBROOK MEDIUM (DSMZ Medium 645) | Medium recipe at MediaDive | Name: MIDDLEBROOK MEDIUM (DSMZ Medium 645) Composition: Bacto Middlebrook 7H10 agar 20.9945 g/l Glycerol Distilled water | ||
| 19856 | MB7H10 | Name: Middlebrook 7H10 (MB7H10) Broth Base Composition: amount in percent Middlebrook 7H10 Agar 1.9% Glycerol 0.5% OADC-Enrichment in ml (NL5633) 10ml Comments: Add broth base and glycerol to ddh2O. Adjust volumes. Autoclave. Cool to 42°C. Add OADC-stock. Sterilisation: 20 minutes Temperature:121°C PH before: PH after: Biological details Culture type: suitable for: Mycobacteria OADC stock solution old broth-name: OADC-Stock Composition: Bovine Serum Albumin, fraction V 5 % 1% Oleic Acid in 0.2N NaOH 5ml Glucose 2 % NaCl 0.85 % Comments: Melt 1% Oleic Acid in 65°C water bath. Dissolve all ingredients in 700ml of ddH2O. Adjust volume to 1L. Filter sterilize and store at 4°C. -To make 1% Oleic Acid in 0.2N NaOH: Dissolve 5g oleic acid (ampule) in 500ml 0.2N NaOH (300ml ddH2O + 20ml NaOH + adjust volume to 500ml). Heat solution to 55°C for oleic acid to melt. Store at -20°C in 50ml aliquots. There is no need to sterilize this. Sterilisation: 20 minutes Temperature:121°C PH before: PH after: Biological details Culture type: suitable for: Mycobacteria | |||
| 19856 | MB7H11 | Name: Middlebrook 7H11 (MB7H11) Broth Base Composition: Middlebrook 7H11 Agar 2% Glycerol 0.5% OADC-Enrichment 10ml Comments: Add broth base and glycerol to ddH2O. Adjust volumes. Autoclave. Cool to 42°C. Add OADC-Stock. Sterilisation: 20 minutes Temperature:121°C PH before: PH after: Biological details Culture type: suitable for: Mycobacteria OADC stock solution old broth-name: OADC-Stock Composition: Bovine Serum Albumin, fraction V 5 % 1% Oleic Acid in 0.2N NaOH 5ml Glucose 2 % NaCl 0.85 % Comments: Melt 1% Oleic Acid in 65°C water bath. Dissolve all ingredients in 700ml of ddH2O. Adjust volume to 1L. Filter sterilize and store at 4°C. -To make 1% Oleic Acid in 0.2N NaOH: Dissolve 5g oleic acid (ampule) in 500ml 0.2N NaOH (300ml ddH2O + 20ml NaOH + adjust volume to 500ml). Heat solution to 55°C for oleic acid to melt. Store at -20°C in 50ml aliquots. There is no need to sterilize this. Sterilisation: 20 minutes Temperature:121°C PH before: PH after: Biological details Culture type: suitable for: Mycobacteria | |||
| 19856 | 5006 | Medium: 5006 Name: Composition (g/l) Sucrose 3,0 Dextrin 15,0 Meat extract 1,0 Yeast extract 2,0 Tryptone soy broth (Oxoid) 5,0 NaCl 0,5 K2HPO4 0,5 MgSO4 x 7 H2O 0,5 FeSO4 x 7 H2O 0,01 Agar 20,0 Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7,3 Usage: Maintenance Organisms: All Actinomycetes | |||
| 36362 | MEDIUM 55 - for Mycobacterium | ||||
| 121943 | CIP Medium 55 | Medium recipe at CIP |
| @ref | Chebi-ID | Metabolite | Utilization activity | Kind of utilization tested | |
|---|---|---|---|---|---|
| 68379 | 17634 ChEBI | D-glucose | - | fermentation | from API Coryne |
| 68379 | 16899 ChEBI | D-mannitol | - | fermentation | from API Coryne |
| 68379 | 16988 ChEBI | D-ribose | - | fermentation | from API Coryne |
| 68379 | 65327 ChEBI | D-xylose | - | fermentation | from API Coryne |
| 68379 | 4853 ChEBI | esculin | + | hydrolysis | from API Coryne |
| 68379 | 5291 ChEBI | gelatin | - | hydrolysis | from API Coryne |
| 68379 | 28087 ChEBI | glycogen | - | fermentation | from API Coryne |
| 121943 | 606565 ChEBI | hippurate | + | hydrolysis | |
| 68379 | 17716 ChEBI | lactose | - | fermentation | from API Coryne |
| 68379 | 17306 ChEBI | maltose | - | fermentation | from API Coryne |
| 68379 | 17632 ChEBI | nitrate | - | reduction | from API Coryne |
| 121943 | 17632 ChEBI | nitrate | - | reduction | |
| 121943 | 17632 ChEBI | nitrate | - | respiration | |
| 121943 | 16301 ChEBI | nitrite | - | reduction | |
| 68379 | 17992 ChEBI | sucrose | - | fermentation | from API Coryne |
| 68379 | 16199 ChEBI | urea | - | hydrolysis | from API Coryne |
| @ref | Metabolite | Is sensitive | Is resistant | |
|---|---|---|---|---|
| 121943 | 0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate) |
| @ref | Value | Activity | Ec | |
|---|---|---|---|---|
| 68382 | acid phosphatase | + | 3.1.3.2 | from API zym |
| 68379 | alkaline phosphatase | + | 3.1.3.1 | from API Coryne |
| 68382 | alpha-chymotrypsin | - | 3.4.21.1 | from API zym |
| 68382 | alpha-fucosidase | - | 3.2.1.51 | from API zym |
| 68382 | alpha-galactosidase | - | 3.2.1.22 | from API zym |
| 68379 | alpha-glucosidase | - | 3.2.1.20 | from API Coryne |
| 68382 | alpha-mannosidase | - | 3.2.1.24 | from API zym |
| 121943 | amylase | - | ||
| 121943 | beta-galactosidase | - | 3.2.1.23 | |
| 68379 | beta-galactosidase | - | 3.2.1.23 | from API Coryne |
| 68382 | beta-glucosidase | + | 3.2.1.21 | from API zym |
| 68379 | beta-glucosidase | + | 3.2.1.21 | from API Coryne |
| 68382 | beta-glucuronidase | - | 3.2.1.31 | from API zym |
| 68379 | beta-glucuronidase | - | 3.2.1.31 | from API Coryne |
| 121943 | caseinase | - | 3.4.21.50 | |
| 121943 | catalase | + | 1.11.1.6 | |
| 121943 | DNase | - | ||
| 68382 | esterase (C 4) | + | from API zym | |
| 68382 | esterase lipase (C 8) | + | from API zym | |
| 121943 | gamma-glutamyltransferase | - | 2.3.2.2 | |
| 121943 | gelatinase | - | ||
| 68379 | gelatinase | - | from API Coryne | |
| 121943 | lecithinase | - | ||
| 68382 | leucine arylamidase | + | 3.4.11.1 | from API zym |
| 121943 | lipase | - | ||
| 68382 | N-acetyl-beta-glucosaminidase | - | 3.2.1.52 | from API zym |
| 68379 | N-acetyl-beta-glucosaminidase | - | 3.2.1.52 | from API Coryne |
| 121943 | oxidase | - | ||
| 121943 | protease | - | ||
| 68379 | pyrazinamidase | - | 3.5.1.B15 | from API Coryne |
| 68379 | pyrrolidonyl arylamidase | - | 3.4.19.3 | from API Coryne |
| 121943 | tween esterase | - | ||
| 121943 | urease | - | 3.5.1.5 | |
| 68379 | urease | - | 3.5.1.5 | from API Coryne |
| Cat1 | Cat2 | Cat3 | |
|---|---|---|---|
| #Host | #Mammals | #Suidae (Pig,Swine) | |
| #Host Body-Site | #Organ | #Lymph node |
Global distribution of 16S sequence AF480588 (>99% sequence identity) for Mycolicibacterium from Microbeatlas ![]()
| @ref | Description | Assembly level | INSDC accession | BV-BRC accession | IMG accession | NCBI tax ID | Score | |
|---|---|---|---|---|---|---|---|---|
| 66792 | ASM2582310v1 assembly for Mycolicibacterium porcinum DSM 44242 | contig | 39693 | 71.56 |
| @ref | Description | Accession | Length | Database | NCBI tax ID | |
|---|---|---|---|---|---|---|
| 20218 | Mycobacterium porcinum 16S ribosomal RNA gene, partial sequence | AF480588 | 1456 | 39693 | ||
| 20218 | Mycobacterium porcinum strain DSM 44242 16S ribosomal RNA gene, partial sequence | AF547958 | 540 | 39693 | ||
| 11617 | Mycolicibacterium porcinum strain CIP 105392 16S ribosomal RNA gene, partial sequence | AY457077 | 1483 | 39693 |
| @ref | Trait | Model | Prediction | Confidence in % | In training data |
|---|---|---|---|---|---|
| 125439 | spore_formation | BacteriaNetⓘ | no | 66.00 | no |
| 125439 | motility | BacteriaNetⓘ | no | 70.40 | no |
| 125439 | gram_stain | BacteriaNetⓘ | positive | 89.50 | no |
| 125439 | oxygen_tolerance | BacteriaNetⓘ | obligate aerobe | 96.80 | no |
| @ref | Trait | Model | Prediction | Confidence in % | In training data |
|---|---|---|---|---|---|
| 125438 | gram-positive | gram-positiveⓘ | yes | 93.56 | no |
| 125438 | anaerobic | anaerobicⓘ | no | 98.72 | no |
| 125438 | aerobic | aerobicⓘ | yes | 88.86 | yes |
| 125438 | spore-forming | spore-formingⓘ | no | 62.37 | no |
| 125438 | thermophilic | thermophileⓘ | no | 97.50 | yes |
| 125438 | flagellated | motile2+ⓘ | no | 89.60 | no |
| Topic | Title | Authors | Journal | DOI | Year | |
|---|---|---|---|---|---|---|
| Phylogeny | Phylogenomics and Comparative Genomic Studies Robustly Support Division of the Genus Mycobacterium into an Emended Genus Mycobacterium and Four Novel Genera. | Gupta RS, Lo B, Son J. | Front Microbiol | 10.3389/fmicb.2018.00067 | 2018 | |
| Mycobacterium porcinum causing panniculitis in the cat. | Cox A, Udenberg TJ. | Can Vet J | 2020 | |||
| In Vitro Antimicrobial Activities of Tigecycline, Eravacycline, Omadacycline, and Sarecycline against Rapidly Growing Mycobacteria. | Zhang T, Du J, Dong L, Wang F, Zhao L, Jia J, Wang C, Cheng M, Yu X, Huang H. | Microbiol Spectr | 10.1128/spectrum.03238-22 | 2023 | ||
| Pathogenicity | In vitro activities of tigecycline and 10 other antimicrobials against nonpigmented rapidly growing mycobacteria. | Fernandez-Roblas R, Martin-de-Hijas NZ, Fernandez-Martinez AI, Garcia-Almeida D, Gadea I, Esteban J. | Antimicrob Agents Chemother | 10.1128/aac.00695-08 | 2008 | |
| Pathogenicity | In Vitro Activities of Bedaquiline and Delamanid against Nontuberculous Mycobacteria Isolated in Beijing, China. | Yu X, Gao X, Li C, Luo J, Wen S, Zhang T, Ma Y, Dong L, Wang F, Huang H. | Antimicrob Agents Chemother | 10.1128/aac.00031-19 | 2019 | |
| Enzymology | Five-year outbreak of community- and hospital-acquired Mycobacterium porcinum infections related to public water supplies. | Brown-Elliott BA, Wallace RJ, Tichindelean C, Sarria JC, McNulty S, Vasireddy R, Bridge L, Mayhall CG, Turenne C, Loeffelholz M. | J Clin Microbiol | 10.1128/jcm.05122-11 | 2011 | |
| Enzymology | New PCR systems to confirm real-time PCR detection of Mycobacterium avium subsp. paratuberculosis. | Herthnek D, Bolske G. | BMC Microbiol | 10.1186/1471-2180-6-87 | 2006 | |
| Phylogeny | Rapid identification of mycobacterial whole cells in solid and liquid culture media by matrix-assisted laser desorption ionization-time of flight mass spectrometry. | Lotz A, Ferroni A, Beretti JL, Dauphin B, Carbonnelle E, Guet-Revillet H, Veziris N, Heym B, Jarlier V, Gaillard JL, Pierre-Audigier C, Frapy E, Berche P, Nassif X, Bille E. | J Clin Microbiol | 10.1128/jcm.01397-10 | 2010 | |
| Pathogenicity | [Microbiological properties of M. porcinum isolated from a patient with impairments in IL-12/IFN-gamma pathway]. | Kazumi Y, Maeda S, Udagawa T, Sugawara I, Kamiya H | Kekkaku | 2008 | ||
| Phylogeny | Clinical and laboratory features of Mycobacterium porcinum. | Wallace RJ Jr, Brown-Elliott BA, Wilson RW, Mann L, Hall L, Zhang Y, Jost KC Jr, Brown JM, Kabani A, Schinsky MF, Steigerwalt AG, Crist CJ, Roberts GD, Blacklock Z, Tsukamura M, Silcox V, Turenne C | J Clin Microbiol | 10.1128/JCM.42.12.5689-5697.2004 | 2004 | |
| Enzymology | Distribution of a novel mycolic acid in species of the genus Mycobacterium. | Luquin M, Laneelle MA, Ausina V, Garcia Barcelo M, Belda F, Alonso C, Prats G | Int J Syst Bacteriol | 10.1099/00207713-41-3-390 | 1991 |
| #11617 | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 44242 |
| #19856 | Wink, J.: Compendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig . |
| #20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 ) |
| #20218 | Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 ) |
| #36362 | ; Curators of the CIP; |
| #53801 | Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 37674 |
| #66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) . |
| #67770 | Japan Collection of Microorganism (JCM) ; Curators of the JCM; |
| #68379 | Automatically annotated from API Coryne . |
| #68382 | Automatically annotated from API zym . |
| #69479 | João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta . |
| #121943 | Collection of Institut Pasteur ; Curators of the CIP; CIP 105392 |
| #125438 | Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 ) |
| #125439 | Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 . |
| #126262 | A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 ) |
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