Strain identifier
BacDive ID: 8471
Type strain:
Species: Mycobacterium porcinum
Strain Designation: E10241-1
Strain history: CIP <- 1999, ATCC <- M. Tsukamura: strain E10241-1 <- H. Nemoto
NCBI tax ID(s): 39693 (species)
General
@ref: 11617
BacDive-ID: 8471
DSM-Number: 44242
keywords: genome sequence, 16S sequence, Bacteria, aerobe, mesophilic, rod-shaped, human pathogen
description: Mycobacterium porcinum E10241-1 is an aerobe, mesophilic, rod-shaped human pathogen that was isolated from swine lymph node.
NCBI tax id
- NCBI tax id: 39693
- Matching level: species
strain history
@ref | history |
---|---|
11617 | <- A. F. Yassin <- ATCC <- M. Tsukamura, E 10241-1 <- H. Nemoto, Tachikawa 96 |
67770 | M. Tsukamura 19508 <-- H. Nemoto strain Tachikawa 96. |
121943 | CIP <- 1999, ATCC <- M. Tsukamura: strain E10241-1 <- H. Nemoto |
doi: 10.13145/bacdive8471.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/actinomycetota
- domain: Bacteria
- phylum: Actinomycetota
- class: Actinomycetes
- order: Mycobacteriales
- family: Mycobacteriaceae
- genus: Mycobacterium
- species: Mycobacterium porcinum
- full scientific name: Mycobacterium porcinum Tsukamura et al. 1983
synonyms
- @ref: 20215
- synonym: Mycolicibacterium porcinum
@ref: 11617
domain: Bacteria
phylum: Actinobacteria
class: Actinobacteria
order: Actinomycetales
family: Mycobacteriaceae
genus: Mycobacterium
species: Mycobacterium porcinum
full scientific name: Mycobacterium porcinum Tsukamura et al. 1983
strain designation: E10241-1
type strain: yes
Morphology
cell morphology
- @ref: 121943
- cell shape: rod-shaped
- motility: no
colony morphology
@ref | colony color | incubation period | medium used |
---|---|---|---|
19856 | Light ivory | 10-14 days | 5006 |
19856 | Light ivory | 10-14 days | MB7H10 |
19856 | Light ivory | 10-14 days | MB7H11 |
53801 | 2 days |
Culture and growth conditions
culture medium
@ref | name | growth | link | composition |
---|---|---|---|---|
11617 | MYCOBACTERIUM MEDIUM (DSMZ Medium 219) | yes | https://mediadive.dsmz.de/medium/219 | Name: MYCOBACTERIUM MEDIUM (DSMZ Medium 219) Composition: Agar 20.0 g/l Na2HPO4 x 12 H2O 2.5 g/l Yeast extract 2.0 g/l Proteose peptone no. 3 2.0 g/l Casein peptone 2.0 g/l Sodium citrate 1.5 g/l KH2PO4 1.0 g/l MgSO4 x 7 H2O 0.6 g/l Tween 0.5 g/l Glycerol Distilled water |
11617 | MIDDLEBROOK MEDIUM (DSMZ Medium 645) | yes | https://mediadive.dsmz.de/medium/645 | Name: MIDDLEBROOK MEDIUM (DSMZ Medium 645) Composition: Bacto Middlebrook 7H10 agar 20.9945 g/l Glycerol Distilled water |
19856 | MB7H10 | yes | Name: Middlebrook 7H10 (MB7H10) Broth Base Composition: amount in percent Middlebrook 7H10 Agar 1.9% Glycerol 0.5% OADC-Enrichment in ml (NL5633) 10ml Comments: Add broth base and glycerol to ddh2O. Adjust volumes. Autoclave. Cool to 42°C. Add OADC-stock. Sterilisation: 20 minutes Temperature:121°C PH before: PH after: Biological details Culture type: suitable for: Mycobacteria OADC stock solution old broth-name: OADC-Stock Composition: Bovine Serum Albumin, fraction V 5 % 1% Oleic Acid in 0.2N NaOH 5ml Glucose 2 % NaCl 0.85 % Comments: Melt 1% Oleic Acid in 65°C water bath. Dissolve all ingredients in 700ml of ddH2O. Adjust volume to 1L. Filter sterilize and store at 4°C. -To make 1% Oleic Acid in 0.2N NaOH: Dissolve 5g oleic acid (ampule) in 500ml 0.2N NaOH (300ml ddH2O + 20ml NaOH + adjust volume to 500ml). Heat solution to 55°C for oleic acid to melt. Store at -20°C in 50ml aliquots. There is no need to sterilize this. Sterilisation: 20 minutes Temperature:121°C PH before: PH after: Biological details Culture type: suitable for: Mycobacteria | |
19856 | MB7H11 | yes | Name: Middlebrook 7H11 (MB7H11) Broth Base Composition: Middlebrook 7H11 Agar 2% Glycerol 0.5% OADC-Enrichment 10ml Comments: Add broth base and glycerol to ddH2O. Adjust volumes. Autoclave. Cool to 42°C. Add OADC-Stock. Sterilisation: 20 minutes Temperature:121°C PH before: PH after: Biological details Culture type: suitable for: Mycobacteria OADC stock solution old broth-name: OADC-Stock Composition: Bovine Serum Albumin, fraction V 5 % 1% Oleic Acid in 0.2N NaOH 5ml Glucose 2 % NaCl 0.85 % Comments: Melt 1% Oleic Acid in 65°C water bath. Dissolve all ingredients in 700ml of ddH2O. Adjust volume to 1L. Filter sterilize and store at 4°C. -To make 1% Oleic Acid in 0.2N NaOH: Dissolve 5g oleic acid (ampule) in 500ml 0.2N NaOH (300ml ddH2O + 20ml NaOH + adjust volume to 500ml). Heat solution to 55°C for oleic acid to melt. Store at -20°C in 50ml aliquots. There is no need to sterilize this. Sterilisation: 20 minutes Temperature:121°C PH before: PH after: Biological details Culture type: suitable for: Mycobacteria | |
19856 | 5006 | yes | Medium: 5006 Name: Composition (g/l) Sucrose 3,0 Dextrin 15,0 Meat extract 1,0 Yeast extract 2,0 Tryptone soy broth (Oxoid) 5,0 NaCl 0,5 K2HPO4 0,5 MgSO4 x 7 H2O 0,5 FeSO4 x 7 H2O 0,01 Agar 20,0 Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7,3 Usage: Maintenance Organisms: All Actinomycetes | |
36362 | MEDIUM 55 - for Mycobacterium | yes | ||
121943 | CIP Medium 55 | yes | https://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=55 |
culture temp
@ref | growth | type | temperature | range |
---|---|---|---|---|
19856 | positive | optimum | 37 | mesophilic |
11617 | positive | growth | 37 | mesophilic |
36362 | positive | growth | 37 | mesophilic |
53801 | positive | growth | 30-42 | |
67770 | positive | growth | 37 | mesophilic |
121943 | positive | growth | 22-37 | |
121943 | no | growth | 10 | psychrophilic |
121943 | no | growth | 45 | thermophilic |
Physiology and metabolism
oxygen tolerance
@ref | oxygen tolerance |
---|---|
53801 | aerobe |
121943 | obligate aerobe |
metabolite utilization
@ref | Chebi-ID | metabolite | utilization activity | kind of utilization tested |
---|---|---|---|---|
121943 | 606565 | hippurate | + | hydrolysis |
121943 | 17632 | nitrate | - | reduction |
121943 | 16301 | nitrite | - | reduction |
121943 | 17632 | nitrate | - | respiration |
68379 | 17632 | nitrate | - | reduction |
68379 | 4853 | esculin | + | hydrolysis |
68379 | 16199 | urea | - | hydrolysis |
68379 | 5291 | gelatin | - | hydrolysis |
68379 | 17634 | D-glucose | - | fermentation |
68379 | 16988 | D-ribose | - | fermentation |
68379 | 65327 | D-xylose | - | fermentation |
68379 | 16899 | D-mannitol | - | fermentation |
68379 | 17306 | maltose | - | fermentation |
68379 | 17716 | lactose | - | fermentation |
68379 | 17992 | sucrose | - | fermentation |
68379 | 28087 | glycogen | - | fermentation |
antibiotic resistance
- @ref: 121943
- metabolite: 0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)
- is antibiotic: yes
- is sensitive: no
- is resistant: yes
metabolite production
- @ref: 121943
- Chebi-ID: 35581
- metabolite: indole
- production: no
enzymes
@ref | value | activity | ec |
---|---|---|---|
68379 | gelatinase | - | |
68379 | urease | - | 3.5.1.5 |
68379 | beta-glucosidase | + | 3.2.1.21 |
68379 | N-acetyl-beta-glucosaminidase | - | 3.2.1.52 |
68379 | alpha-glucosidase | - | 3.2.1.20 |
68379 | beta-galactosidase | - | 3.2.1.23 |
68379 | beta-glucuronidase | - | 3.2.1.31 |
68379 | alkaline phosphatase | + | 3.1.3.1 |
68379 | pyrrolidonyl arylamidase | - | 3.4.19.3 |
68379 | pyrazinamidase | - | 3.5.1.B15 |
121943 | oxidase | - | |
121943 | beta-galactosidase | - | 3.2.1.23 |
121943 | gelatinase | - | |
121943 | amylase | - | |
121943 | DNase | - | |
121943 | caseinase | - | 3.4.21.50 |
121943 | catalase | + | 1.11.1.6 |
121943 | tween esterase | - | |
121943 | gamma-glutamyltransferase | - | 2.3.2.2 |
121943 | lecithinase | - | |
121943 | lipase | - | |
121943 | protease | - | |
121943 | urease | - | 3.5.1.5 |
68382 | esterase (C 4) | + | |
68382 | esterase lipase (C 8) | + | |
68382 | leucine arylamidase | + | 3.4.11.1 |
68382 | alpha-chymotrypsin | - | 3.4.21.1 |
68382 | acid phosphatase | + | 3.1.3.2 |
68382 | alpha-galactosidase | - | 3.2.1.22 |
68382 | beta-glucuronidase | - | 3.2.1.31 |
68382 | beta-glucosidase | + | 3.2.1.21 |
68382 | N-acetyl-beta-glucosaminidase | - | 3.2.1.52 |
68382 | alpha-mannosidase | - | 3.2.1.24 |
68382 | alpha-fucosidase | - | 3.2.1.51 |
API coryne
@ref | NIT | PYZ | PYRA | PAL | beta GUR | beta GAL | alpha GLU | beta NAG | ESC | URE | GEL | GLU | RIB | XYL | MAN | MAL | LAC | SAC | GLYG |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
19856 | - | - | - | + | - | - | - | - | + | - | - | - | - | - | - | - | - | - | - |
API zym
@ref | Alkaline phosphatase | Esterase | Esterase Lipase | Lipase | Leucine arylamidase | Valine arylamidase | Cystine arylamidase | Trypsin | alpha- Chymotrypsin | Acid phosphatase | Naphthol-AS-BI-phosphohydrolase | alpha- Galactosidase | beta- Galactosidase | beta- Glucuronidase | alpha- Glucosidase | beta- Glucosidase | N-acetyl-beta- glucosaminidase | alpha- Mannosidase | alpha- Fucosidase | Control |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
19856 | - | + | + | - | + | - | - | - | - | + | - | - | - | - | - | + | - | - | - | |
121943 | + | + | + | + | + | + | + | + | - | + | + | - | + | - | + | + | - | - | - | - |
API biotype100
@ref | GLU | FRU | GAL | TRE | MNE | SBE | MEL | SAC | RAF | MTE | MAL | LAC | LTE | MbGa | MaGa | CEL | GEN | MbGu | ESC | RIB | ARA | XYL | PLE | RHA | FUC | MLZ | DARL | LARL | XLT | DUL | TAG | GLY | INO | MAN | MTL | TUR | SOR | ADO | HBG | LYX | ERY | MDG | 3MDG | SAT | MUC | LTAT | DTAT | MTAT | DMLT | LMLT | CATE | TATE | TTE | CIT | GRT | GAT | 2KG | 5KG | TRY | NAG | GNT | PAC | PAT | pOBE | QAT | GTE | mOBE | BAT | PPAT | CMT | TGE | BET | PCE | ABT | HIN | LAT | CAP | CYT | HIS | SUC | FUM | GRE | GYT | AVT | ETN | TTN | GLN | ITA | 3OBU | APT | GTT | PRO | DALA | LALA | SER | MNT | PROP | TYR | 2KT |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
121943 | + | + | - | + | + | - | - | - | - | - | - | - | - | - | - | - | - | - | + | - | - | - | - | - | - | - | - | - | - | - | - | + | + | + | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | + | + | + | - | - | - | - | - | - | - | - | + | - | - | - | - | - | - | + | - | - | - | - | + | + | + | + | - | + | + | + | + | - | - | + | + | - | + | - | + | - | + | + | + | + | + | - | + | - | - |
Isolation, sampling and environmental information
isolation
@ref | sample type |
---|---|
11617 | swine lymph node |
53801 | Swine lymph node,lymphadenitis |
67770 | Swine lymphadenitis |
121943 | Animal, Swine, lymph node |
isolation source categories
Cat1 | Cat2 | Cat3 |
---|---|---|
#Host | #Mammals | #Suidae (Pig,Swine) |
#Host Body-Site | #Organ | #Lymph node |
taxonmaps
- @ref: 69479
- File name: preview.99_515.png
- url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_10;96_115;97_219;98_419;99_515&stattab=map
- Last taxonomy: Mycolicibacterium
- 16S sequence: AF480588
- Sequence Identity:
- Total samples: 496
- soil counts: 45
- aquatic counts: 64
- animal counts: 50
- plant counts: 337
Safety information
risk assessment
@ref | pathogenicity human | pathogenicity animal | biosafety level | biosafety level comment |
---|---|---|---|---|
11617 | yes | yes | 2 | Risk group (German classification) |
19856 | 2 | Risk group (German classification) | ||
121943 | 1 | Risk group (French classification) |
Sequence information
16S sequences
@ref | description | accession | length | database | NCBI tax ID |
---|---|---|---|---|---|
20218 | Mycobacterium porcinum 16S ribosomal RNA gene, partial sequence | AF480588 | 1456 | ena | 39693 |
20218 | Mycobacterium porcinum strain DSM 44242 16S ribosomal RNA gene, partial sequence | AF547958 | 540 | ena | 39693 |
11617 | Mycolicibacterium porcinum strain CIP 105392 16S ribosomal RNA gene, partial sequence | AY457077 | 1483 | ena | 39693 |
Genome sequences
- @ref: 66792
- description: Mycolicibacterium porcinum DSM 44242
- accession: GCA_025823105
- assembly level: contig
- database: ncbi
- NCBI tax ID: 39693
Genome-based predictions
predictions
trait | prediction | confidence | training_data |
---|---|---|---|
motile | no | 94.791 | no |
flagellated | no | 98.006 | no |
gram-positive | yes | 90.599 | no |
anaerobic | no | 99.455 | yes |
aerobic | yes | 90.867 | yes |
halophile | no | 93.364 | no |
spore-forming | no | 83.976 | no |
glucose-ferment | no | 90.318 | no |
thermophile | no | 99.651 | yes |
glucose-util | yes | 89.239 | no |
External links
@ref: 11617
culture collection no.: DSM 44242, ATCC 33776, JCM 6378, CCUG 37674, CIP 105392, KCTC 9517
straininfo link
- @ref: 77866
- straininfo: 44973
literature
topic | Pubmed-ID | title | authors | journal | DOI | year | mesh | topic2 |
---|---|---|---|---|---|---|---|---|
Enzymology | 1883713 | Distribution of a novel mycolic acid in species of the genus Mycobacterium. | Luquin M, Laneelle MA, Ausina V, Garcia Barcelo M, Belda F, Alonso C, Prats G | Int J Syst Bacteriol | 10.1099/00207713-41-3-390 | 1991 | Chromatography, Thin Layer, Mycobacterium/*analysis, Mycolic Acids/chemistry/*isolation & purification | Phylogeny |
Phylogeny | 15583300 | Clinical and laboratory features of Mycobacterium porcinum. | Wallace RJ Jr, Brown-Elliott BA, Wilson RW, Mann L, Hall L, Zhang Y, Jost KC Jr, Brown JM, Kabani A, Schinsky MF, Steigerwalt AG, Crist CJ, Roberts GD, Blacklock Z, Tsukamura M, Silcox V, Turenne C | J Clin Microbiol | 10.1128/JCM.42.12.5689-5697.2004 | 2004 | Animals, Anti-Bacterial Agents/pharmacology, Bacterial Proteins/genetics, *Bacterial Typing Techniques, Base Sequence, Chaperonin 60, Chaperonins/genetics, DNA, Ribosomal/analysis, Humans, Microbial Sensitivity Tests, Molecular Sequence Data, Mycobacterium/*classification/drug effects/genetics/pathogenicity, Mycobacterium Infections/microbiology/physiopathology, Mycobacterium fortuitum/classification/drug effects/genetics/pathogenicity, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Swine/*microbiology | Pathogenicity |
Pathogenicity | 19086435 | [Microbiological properties of M. porcinum isolated from a patient with impairments in IL-12/IFN-gamma pathway]. | Kazumi Y, Maeda S, Udagawa T, Sugawara I, Kamiya H | Kekkaku | 2008 | Animals, Anti-Bacterial Agents/pharmacology, Base Sequence, Drug Resistance, Bacterial, Genes, Bacterial/genetics, Genetic Predisposition to Disease/genetics, Guinea Pigs, Humans, *Interferon-gamma, *Interleukin-12, Male, *Mutation, Mycobacterium fortuitum/drug effects/genetics/*isolation & purification/pathogenicity, Receptors, Interferon/*genetics, Signal Transduction/*genetics/physiology, Young Adult | Enzymology |
Reference
@id | authors | catalogue | doi/url | title | journal | pubmed |
---|---|---|---|---|---|---|
11617 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 44242) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-44242 | |||
19856 | Wink, J. | http://www.dsmz.de/microorganisms/wink_pdf/DSM44242.pdf | Compendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig | |||
20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |||
20218 | Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P. | 10.1016/j.syapm.2013.11.002 | StrainInfo introduces electronic passports for microorganisms. | Syst Appl Microbiol. 37: 42-50 2014 | 24321274 | |
36362 | Curators of the CIP | https://brclims.pasteur.fr/brcWeb/souche/detail/1/17406 | ||||
53801 | Curators of the CCUG | Culture Collection University of Gothenburg (CCUG) (CCUG 37674) | https://www.ccug.se/strain?id=37674 | |||
66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |||
67770 | Curators of the JCM | https://jcm.brc.riken.jp/en/ | ||||
68379 | Automatically annotated from API Coryne | |||||
68382 | Automatically annotated from API zym | |||||
69479 | João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich. | https://microbeatlas.org/ | MicrobeAtlas 1.0 beta | |||
77866 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID44973.1 | StrainInfo: A central database for resolving microbial strain identifiers | |||
121943 | Curators of the CIP | Collection of Institut Pasteur (CIP 105392) | https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20105392 |