Mycobacterium neoaurum (DSM 2967, NRRL B-3805)

Multimedia

• @ref:	1263
  multimedia content:	DSM_2967.jpg
  multimedia.multimedia content:	https://www.dsmz.de/microorganisms/photos/DSM_2967.jpg
  caption:	Medium 65 37°C
  intellectual property rights:	© Leibniz-Institut DSMZ
  manual_annotation:	1

Culture medium

@ref	Name	Growth	Medium link	Composition
1263	GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)		Medium recipe at MediaDive	Name: GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) Composition: Agar 18.0 g/l Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l CaCO3 2.0 g/l Distilled water
1263	MIDDLEBROOK MEDIUM (DSMZ Medium 645)		Medium recipe at MediaDive	Name: MIDDLEBROOK MEDIUM (DSMZ Medium 645) Composition: Bacto Middlebrook 7H10 agar 20.9945 g/l Glycerol Distilled water

Culture temp

Compound production

Risk assessment

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
124043	ASM158040v1 assembly for Mycolicibacterium neoaurum NRRL B-3805	complete	GCA_001580405	1570328.3	2684623113	1795	96.06

Literature

Topic	Title	Authors	Journal	DOI	Year
	Biogas digestate as a sustainable phytosterol source for biotechnological cascade valorization.	Weckerle T, Ewald H, Guth P, Knorr KH, Philipp B, Holert J.	Microb Biotechnol	10.1111/1751-7915.14174	2023
	Biotransformation of Phytosterols into Androstenedione-A Technological Prospecting Study.	Nunes VO, Vanzellotti NC, Fraga JL, Pessoa FLP, Ferreira TF, Amaral PFF.	Molecules	10.3390/molecules27103164	2022
Enzymology	Efficient Synthesis of Steroidal Intermediates with a C17 Side Chain from Phytosterols by Genetically Modified Mycolicibacterium neoaurum NRRL B-3805 Strain.	Li X, Zhu L, Wu Q, Zhang R, Liu Y, Liu N, Feng J, Wu Q, Zhu D.	ChemistryOpen	10.1002/open.202500086	2025
	Rerouting phytosterol degradation pathway for directed androst-1,4-diene-3,17-dione microbial bioconversion.	Ke X, Cui JH, Ren QJ, Zheng T, Wang XX, Liu ZQ, Zheng YG.	Appl Microbiol Biotechnol	10.1007/s00253-023-12847-z	2024
Enzymology	Whole-genome and enzymatic analyses of an androstenedione-producing Mycobacterium strain with residual phytosterol-degrading pathways.	Wang H, Song S, Peng F, Yang F, Chen T, Li X, Cheng X, He Y, Huang Y, Su Z.	Microb Cell Fact	10.1186/s12934-020-01442-w	2020
Metabolism	Efficient conversion of phytosterols into 4-androstene-3,17-dione and its C1,2-dehydrogenized and 9alpha-hydroxylated derivatives by engineered Mycobacteria.	Li X, Chen T, Peng F, Song S, Yu J, Sidoine DN, Cheng X, Huang Y, He Y, Su Z.	Microb Cell Fact	10.1186/s12934-021-01653-9	2021
	Biochemical characterization of acyl-coenzyme A synthetases involved in mycobacterial steroid side-chain catabolism and molecular design: synthesis of an anti-mycobacterial agent.	Niu Y, Ge F, Yang Y, Ren Y, Li W, Chen G, Wen D, Liu F, Xiong L.	3 Biotech	10.1007/s13205-019-1703-y	2019
Metabolism	Improving the production of 22-hydroxy-23,24-bisnorchol-4-ene-3-one from sterols in Mycobacterium neoaurum by increasing cell permeability and modifying multiple genes.	Xiong LB, Liu HH, Xu LQ, Sun WJ, Wang FQ, Wei DZ.	Microb Cell Fact	10.1186/s12934-017-0705-x	2017
Enzymology	Bioproduction of testosterone from phytosterol by Mycolicibacterium neoaurum strains: "one-pot", two modes.	Tekucheva DN, Nikolayeva VM, Karpov MV, Timakova TA, Shutov AV, Donova MV.	Bioresour Bioprocess	10.1186/s40643-022-00602-7	2022
Phylogeny	Optimization of biotransformation from phytosterol to androstenedione by a mutant Mycobacterium neoaurum ZJUVN-08.	Zhang XY, Peng Y, Su ZR, Chen QH, Ruan H, He GQ.	J Zhejiang Univ Sci B	10.1631/jzus.b1200067	2013
Metabolism	Mycobacterium smegmatis is a suitable cell factory for the production of steroidic synthons.	Galan B, Uhia I, Garcia-Fernandez E, Martinez I, Bahillo E, de la Fuente JL, Barredo JL, Fernandez-Cabezon L, Garcia JL.	Microb Biotechnol	10.1111/1751-7915.12429	2017
Metabolism	New product identification in the sterol metabolism by an industrial strain Mycobacterium neoaurum NRRL B-3805.	Li X, Chen X, Wang Y, Yao P, Zhang R, Feng J, Wu Q, Zhu D, Ma Y	Steroids	10.1016/j.steroids.2018.02.001	2018
Metabolism	Genetic Techniques for Manipulation of the Phytosterol Biotransformation Strain Mycobacterium neoaurum NRRL B-3805.	Loraine JK, Smith MCM	Methods Mol Biol	10.1007/978-1-4939-7183-1_7	2017
Proteome	Intra- and Extra-cellular Proteome Analyses of Steroid-Producer Mycobacteria.	Barreiro C, Morales A, Vazquez-Iglesias I, Sola-Landa A	Methods Mol Biol	10.1007/978-1-4939-7183-1_6	2017
Genetics	Genome Sequencing of Steroid Producing Bacteria Using Ion Torrent Technology and a Reference Genome.	Sola-Landa A, Rodriguez-Garcia A, Barreiro C, Perez-Redondo R	Methods Mol Biol	10.1007/978-1-4939-7183-1_4	2017
Genetics	Complete genome sequence of 'Mycobacterium neoaurum' NRRL B-3805, an androstenedione (AD) producer for industrial biotransformation of sterols.	Rodriguez-Garcia A, Fernandez-Alegre E, Morales A, Sola-Landa A, Lorraine J, Macdonald S, Dovbnya D, Smith MC, Donova M, Barreiro C	J Biotechnol	10.1016/j.jbiotec.2016.03.021	2016