Mycobacterium neoaurum (DSM 1381, MCI-0617)

Cell morphology

Multimedia

• @ref:	756
  multimedia content:	DSM_1381.jpg
  multimedia.multimedia content:	https://www.dsmz.de/microorganisms/photos/DSM_1381.jpg
  caption:	Medium 645 37°C
  intellectual property rights:	© Leibniz-Institut DSMZ
  manual_annotation:	1

Culture medium

@ref	Name	Growth	Medium link	Composition
756	MIDDLEBROOK MEDIUM (DSMZ Medium 645)		Medium recipe at MediaDive	Name: MIDDLEBROOK MEDIUM (DSMZ Medium 645) Composition: Bacto Middlebrook 7H10 agar 20.9945 g/l Glycerol Distilled water
756	LÖWENSTEIN-JENSEN MEDIUM (DSMZ Medium 354)		Medium recipe at MediaDive	Name: LÖWENSTEIN-JENSEN MEDIUM (DSMZ Medium 354) Composition: Potato flour 18.6104 g/l L-Asparagin 2.23325 g/l KH2PO4 1.55087 g/l Mg-citrate 0.372208 g/l Malachite green 0.248139 g/l MgSO4 0.148883 g/l Glycerol Fresh egg mixture Distilled water

Culture temp

Oxygen tolerance

Risk assessment

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	NCBI tax ID	Score
66792	ASM2785701v1 assembly for Mycolicibacterium neoaurum DSM 1381	chromosome	GCA_027857015	1795.28	1795	88.65

Predictions

Literature

Topic	Title	Authors	Journal	DOI	Year
Genetics	Whole-Genome Analysis of Mycobacterium neoaurum DSM 1381 and the Validation of Two Key Enzymes Affecting C22 Steroid Intermediates in Sterol Metabolism.	Zhang J, Zhang R, Song S, Su Z, Shi J, Cao H, Zhang B.	Int J Mol Sci	10.3390/ijms24076148	2023
Metabolism	Efficient conversion of phytosterols into 4-androstene-3,17-dione and its C1,2-dehydrogenized and 9alpha-hydroxylated derivatives by engineered Mycobacteria.	Li X, Chen T, Peng F, Song S, Yu J, Sidoine DN, Cheng X, Huang Y, He Y, Su Z.	Microb Cell Fact	10.1186/s12934-021-01653-9	2021
	Efficient Production of 9,22-Dihydroxy-23,24-bisnorchol-4-ene-3-one from Phytosterols by Modifying Multiple Genes in Mycobacterium fortuitum.	Han S, Liu X, He B, Zhai X, Yuan C, Li Y, Lin W, Wang H, Zhang B.	Int J Mol Sci	10.3390/ijms25073579	2024
Enzymology	Loop pathways are responsible for tuning the accumulation of C19- and C22-sterol intermediates in the mycobacterial phytosterol degradation pathway.	Song S, He J, Gao M, Huang Y, Cheng X, Su Z.	Microb Cell Fact	10.1186/s12934-022-02008-8	2023
	Rational development of mycobacteria cell factory for advancing the steroid biomanufacturing.	Wang XX, Ke X, Liu ZQ, Zheng YG.	World J Microbiol Biotechnol	10.1007/s11274-022-03369-3	2022
Enzymology	Purification, characterization, and application of a high activity 3-ketosteroid-Delta(1)-dehydrogenase from Mycobacterium neoaurum DSM 1381.	Zhang R, Xu X, Cao H, Yuan C, Yuminaga Y, Zhao S, Shi J, Zhang B	Appl Microbiol Biotechnol	10.1007/s00253-019-09988-5	2019
Metabolism	Identification, function, and application of 3-ketosteroid Delta1-dehydrogenase isozymes in Mycobacterium neoaurum DSM 1381 for the production of steroidic synthons.	Zhang R, Liu X, Wang Y, Han Y, Sun J, Shi J, Zhang B	Microb Cell Fact	10.1186/s12934-018-0916-9	2018