Name: Psychrobacter aquaticus DSM 15339
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 8198

Type strain:

Species: Psychrobacter aquaticus

Culture col. no.: DSM 15339, MTCC 4386, CMS 56

Strain history: <- S. Shivaji; CMS56

NCBI tax ID(s): 1354303 (strain), 248452 (species)

Section

Psychrobacter aquaticus DSM 15339 is an aerobe, psychrophilic, Gram-negative bacterium that forms circular colonies and was isolated from cyanobacterial mat sample.

coccus-shaped
colony-forming
Gram-negative
aerobe
psychrophilic
16S sequence
Bacteria

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	07-12-2022 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Pseudomonadota*
	Class	*Gammaproteobacteria*
	Order	*Pseudomonadales*
	Family	*Moraxellaceae*
	Genus	*Psychrobacter*
	Species	**Psychrobacter aquaticus**
	Full Scientific Name (PNU)	Psychrobacter aquaticus Shivaji et al. 2005

Information on morphological and physiological properties Morphology

[Ref.: #30176]	Gram stain	negative
[Ref.: #30176]	Cell shape	coccus-shaped
[Ref.: #30176]	Motility	no

[Ref.: #26525]	Cell shape	coccus-shaped
[Ref.: #26525]	Motility	no

[Ref.: #26525]	Colony size	2-3 mm
[Ref.: #26525]	Colony shape	circular
[Ref.: #26525]	Cultivation medium used	ZoBell Marine 2296 agar

[Ref.: #30176]

Pigment production

Information on culture and growth conditions Culture and growth conditions

[Ref.: #5854]

Culture medium

OXOID NUTRIENT BROTH (DSMZ Medium 948)

[Ref.: #5854]

Culture medium growth

[Ref.: #5854]

Culture medium link

Medium recipe provided by DSMZ

[Ref.: #5854]

Culture medium composition

expand / minimize

[Ref.: #5854]

Culture medium

NUTRIENT AGAR (OXOID CM3) (DSMZ Medium 605)

[Ref.: #5854]

Culture medium growth

[Ref.: #5854]

Culture medium link

Medium recipe provided by DSMZ

[Ref.: #5854]

Culture medium composition

expand / minimize

[Ref.: #5854]

Culture medium

LB (Luria-Bertani) MEDIUM (DSMZ Medium 381)

[Ref.: #5854]

Culture medium growth

[Ref.: #5854]

Culture medium link

Medium recipe provided by DSMZ

[Ref.: #5854]

Culture medium composition

expand / minimize

[Ref.: #38181]

Culture medium

MEDIUM 72- for trypto casein soja agar

[Ref.: #38181]

Culture medium growth

[Ref.: #38181]

Culture medium composition

expand / minimize

[Ref.: #26525]

Culture medium

ZoBell Marine 2296 agar

[Ref.: #26525]

Culture medium growth

		Temperature
[Ref.: #5854]	growth	22 °C
[Ref.: #26525]	optimum	22 °C
[Ref.: #26525]	growth	4-30 °C
[Ref.: #30176]	growth	04-30 °C
[Ref.: #30176]	optimum	22 °C
[Ref.: #38181]	growth	22 °C

[Ref.: #5854]	Temperature range	psychrophilic
[Ref.: #26525]	Temperature range	psychrophilic
[Ref.: #30176]	Temperature range	psychrophilic
[Ref.: #38181]	Temperature range	psychrophilic

Information on physiology and metabolism Physiology and metabolism

[Ref.: #26525]

Oxygen tolerance

aerobe

	Salt		Salt conc.
[Ref.: #30176]	NaCl	growth	0-10	%
[Ref.: #30176]	NaCl	optimum	5	%
[Ref.: #26525]	NaCl	growth	0-10	%

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #26525]	acetate ChEBI	-	carbon source
[Ref.: #30176]	alanine ChEBI	+	carbon source
[Ref.: #26525]	arginine ChEBI	-	hydrolysis
[Ref.: #30176]	asparagine ChEBI	+	carbon source
[Ref.: #26525]	butanol ChEBI	-	carbon source
[Ref.: #43400]	cellobiose ChEBI	-	degradation
[Ref.: #26525]	cellulose ChEBI	-	carbon source
[Ref.: #26525]	cellulose ChEBI	-	degradation
[Ref.: #43400]	cellulose ChEBI	-	degradation
[Ref.: #26525]	citrate ChEBI	-	carbon source
[Ref.: #26525]	D-fructose ChEBI	-	carbon source
[Ref.: #26525]	D-glucose ChEBI	-	builds acid from
[Ref.: #43400]	D-glucose ChEBI	+	assimilation
[Ref.: #26525]	D-mannitol ChEBI	-	carbon source
[Ref.: #26525]	D-mannose ChEBI	-	carbon source
[Ref.: #26525]	D-mannose ChEBI	+	builds acid from
[Ref.: #26525]	D-ribose ChEBI	-	carbon source
[Ref.: #26525]	D-xylose ChEBI	-	carbon source
[Ref.: #26525]	dextran ChEBI	-	carbon source
[Ref.: #26525]	erythritol ChEBI	-	carbon source
[Ref.: #26525]	esculin ChEBI	-	hydrolysis
[Ref.: #26525]	fumarate ChEBI	-	carbon source
[Ref.: #26525]	galactitol ChEBI	-	carbon source
[Ref.: #26525]	gelatin ChEBI	-	hydrolysis
[Ref.: #26525]	glucose ChEBI	-	fermentation
[Ref.: #26525]	glucose ChEBI	-	carbon source
[Ref.: #30176]	glutamate ChEBI	+	carbon source
[Ref.: #30176]	glycerol ChEBI	+	carbon source
[Ref.: #26525]	glycerol ChEBI	+	carbon source
[Ref.: #26525]	hexadecane ChEBI	-	carbon source
[Ref.: #26525]	inulin ChEBI	-	carbon source
[Ref.: #26525]	L-alanine 4-nitroanilide	+	carbon source
[Ref.: #26525]	L-arabinose ChEBI	-	carbon source
[Ref.: #26525]	L-arabinose ChEBI	-	builds acid from
[Ref.: #26525]	L-arginine ChEBI	-	carbon source
[Ref.: #26525]	L-asparagine ChEBI	-	carbon source
[Ref.: #26525]	L-asparagine ChEBI	+	carbon source
[Ref.: #26525]	L-cysteine ChEBI	-	carbon source
[Ref.: #26525]	L-glutamate ChEBI	+	carbon source
[Ref.: #26525]	L-glutamine ChEBI	-	carbon source
[Ref.: #26525]	L-histidine ChEBI	-	carbon source
[Ref.: #26525]	L-isoleucine ChEBI	-	carbon source
[Ref.: #26525]	L-leucine ChEBI	-	carbon source
[Ref.: #26525]	L-methionine ChEBI	-	carbon source
[Ref.: #26525]	L-phenylalanine ChEBI	+	carbon source
[Ref.: #43400]	L-rhamnose ChEBI	+	assimilation
[Ref.: #26525]	L-serine ChEBI	-	carbon source
[Ref.: #26525]	L-tryptophan ChEBI	-	carbon source
[Ref.: #26525]	L-tyrosine ChEBI	-	carbon source
[Ref.: #26525]	L-tyrosine ChEBI	+	carbon source
[Ref.: #26525]	L-valine ChEBI	-	carbon source
[Ref.: #26525]	lactate ChEBI	-	carbon source
[Ref.: #26525]	lactose ChEBI	-	carbon source
[Ref.: #26525]	lactose ChEBI	-	builds acid from
[Ref.: #26525]	maltose ChEBI	-	carbon source
[Ref.: #43400]	maltose ChEBI	+	assimilation
[Ref.: #43400]	maltotriose ChEBI	+	assimilation
[Ref.: #30176]	mannose ChEBI	+	carbon source
[Ref.: #26525]	melibiose ChEBI	-	carbon source
[Ref.: #26525]	methanol ChEBI	-	carbon source
[Ref.: #26525]	myo-inositol ChEBI	-	carbon source
[Ref.: #30176]	nitrate ChEBI	+	reduction
[Ref.: #26525]	nitrate ChEBI	+	reduction
[Ref.: #30176]	phenylalanine ChEBI	+	carbon source
[Ref.: #26525]	pyruvate ChEBI	-	carbon source
[Ref.: #26525]	ribitol ChEBI	-	carbon source
[Ref.: #26525]	sorbitol ChEBI	-	carbon source
[Ref.: #26525]	starch ChEBI	-	hydrolysis
[Ref.: #26525]	sucrose ChEBI	-	carbon source
[Ref.: #26525]	sucrose ChEBI	-	builds acid from
[Ref.: #26525]	threonine ChEBI	-	carbon source
[Ref.: #30176]	trehalose ChEBI	+	carbon source
[Ref.: #26525]	trehalose ChEBI	-	carbon source
[Ref.: #26525]	xanthine ChEBI	-	carbon source
	Only first 10 entries are displayed. Click here to see all.

	Metabolite	Antibiotic sensitivity	Concentration		Antibiotic resistance	Concentration
[Ref.: #26525]	amoxicillin ChEBI	yes	100	µg
[Ref.: #26525]	ampicillin ChEBI	yes	10	µg
[Ref.: #26525]	bacitracin ChEBI	yes	10	µg
[Ref.: #26525]	carbenicillin ChEBI	yes	50	µg
[Ref.: #26525]	chloramphenicol ChEBI	yes	30	µg
[Ref.: #26525]	co-trimoxazole ChEBI	yes	25	µg
[Ref.: #26525]	colistin ChEBI				yes	10	µg
[Ref.: #26525]	erythromycin ChEBI	yes	15	µg
[Ref.: #26525]	gentamicin ChEBI	yes	10	µg
[Ref.: #26525]	lincomycin ChEBI				yes	2	µg
[Ref.: #26525]	nalidixic acid ChEBI	yes	30	µg
[Ref.: #26525]	nitrofurantoin ChEBI	yes	300	µg
[Ref.: #26525]	nystatin ChEBI	yes	100	µg
[Ref.: #26525]	polymyxin b ChEBI	yes	300	µg
[Ref.: #26525]	rifampicin ChEBI	yes	5	µg
[Ref.: #26525]	streptomycin ChEBI	yes	10	µg
[Ref.: #26525]	tetracycline ChEBI	yes	30	µg
[Ref.: #26525]	tobramycin ChEBI	yes	20	µg
	Only first 10 entries are displayed. Click here to see all.

	Metabolite production	Metabolite
[Ref.: #26525]		acetoin ChEBI
[Ref.: #26525]		hydrogen sulfide ChEBI
[Ref.: #26525]		indole ChEBI

	Metabolite	Voges-Proskauer-test	Methylred-test	Citrate test
[Ref.: #26525]	acetoin ChEBI	-
[Ref.: #26525]	citrate ChEBI			-
[Ref.: #26525]	glucose ChEBI		-

	Enzyme	Enzyme activity	EC number
[Ref.: #30176]	alkaline phosphatase	+	3.1.3.1
[Ref.: #26525]	arginine decarboxylase	-	4.1.1.19
[Ref.: #26525]	beta-galactosidase	-	3.2.1.23
[Ref.: #30176]	catalase	+	1.11.1.6
[Ref.: #26525]	catalase	+	1.11.1.6
[Ref.: #30176]	cytochrome oxidase	+	1.9.3.1
[Ref.: #26525]	cytochrome oxidase	+	1.9.3.1
[Ref.: #26525]	lipase	+
[Ref.: #26525]	lysine decarboxylase	-	4.1.1.18
[Ref.: #26525]	phosphatase	+
[Ref.: #30176]	urease	+	3.5.1.5
[Ref.: #26525]	urease	+	3.5.1.5
	Only first 10 entries are displayed. Click here to see all.

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #5854]	Sample type/isolated from	cyanobacterial mat sample
[Ref.: #5854]	Geographic location (country and/or sea, region)	McMurdo
[Ref.: #5854]	Continent	Australia and Oceania

[Ref.: #26525]	Sample type/isolated from	cyanobacteral mat samples from lake canopus
[Ref.: #26525]	Geographic location (country and/or sea, region)	Mcmurdo Dry Valley Region
[Ref.: #26525]	Country	Antarctica
[Ref.: #26525]	Country ISO 3 Code	ATA
[Ref.: #26525]	Continent	Australia and Oceania
* marker position based on {}

Isolation sources categories

#Environmental	#Microbial community	#Microbial mat
#Host	#Microbial	#Bacteria
#Climate	#Cold	-
#Condition	#Psychrophilic (<10°C)	-
#Environmental	#Aquatic	#Lake (large)

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence AJ584833 (>99% sequence identity) for Psychrobacter from Microbeatlas

Aquatic Samples	834
Soil Samples	114
Animal Samples	378
Plant Samples	37
Total Samples	1363

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #5854]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #30176]	Psychrobacter aquatica partial 16S rRNA gene, isolate CMS 56	AJ584833	1481	GenBank	1354303 tax ID

[Ref.: #5854]	GC-content	43 mol%
[Ref.: #26525]	GC-content	43.6 mol%

Availability in culture collections External links

[Ref.: #5854]

Culture collection no.

DSM 15339, MTCC 4386, CMS 56

[Ref.: #20218]

Associated Passport(s) in StrainInfo

386588, 386589

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Psychrobacter vallis sp. nov. and Psychrobacter aquaticus sp. nov., from Antarctica.	Shivaji S, Reddy GSN, Suresh K, Gupta P, Chintalapati S, Schumann P, Stackebrandt E, Matsumoto GI	Int J Syst Evol Microbiol	10.1099/ijs.0.03030-0	2005	*
Genetics	Draft Genome Sequence of Psychrobacter aquaticus Strain CMS 56T, Isolated from a Cyanobacterial Mat Sample Collected from Water Bodies in the McMurdo Dry Valley Region of Antarctica.	Reddy GS, Ara S, Singh A, Kumar Pinnaka A, Shivaji S	Genome Announc	10.1128/genomeA.00918-13	2013	*

References

#5854

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 15339

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#20218

Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )

#26525

IJSEM 757 2005 ( DOI 10.1099/ijs.0.03030-0 , PubMed 15774658 )

#30176

Barberan A, Caceres Velazquez H, Jones S, Fierer N.: Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information. mSphere 2: 2017 ( DOI 10.1128/mSphere.00237-17 , PubMed 28776041 ) - originally annotated from #26525 (see below)

#38181 ; Curators of the CIP;

#43400

Stephan Hetzler, Daniel Bröker, Alexander Steinbüchel: Saccharification of Cellulose by Recombinant Rhodococcus opacus PD630 Strains. IJSEM 79: 5159 - 5166 2013 ( DOI 10.1128/AEM.01214-13 , PubMed 23793636 )

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#26525

IJSEM 757 2005 ( DOI 10.1099/ijs.0.03030-0 , PubMed 15774658 )

* These data were automatically processed and therefore are not curated

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