Strain identifier

BacDive ID: 8198

Type strain: Yes

Species: Psychrobacter aquaticus

Strain history: CIP <- 2005, MTCC <- S. Shivaji: strain CMS56

NCBI tax ID(s): 1354303 (strain), 248452 (species)

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General

@ref: 5854

BacDive-ID: 8198

DSM-Number: 15339

keywords: 16S sequence, Bacteria, aerobe, psychrophilic, Gram-negative, coccus-shaped, colony-forming

description: Psychrobacter aquaticus DSM 15339 is an aerobe, psychrophilic, Gram-negative bacterium that forms circular colonies and was isolated from cyanobacterial mat sample.

NCBI tax id

NCBI tax idMatching level
1354303strain
248452species

strain history

@refhistory
5854<- S. Shivaji; CMS56
116437CIP <- 2005, MTCC <- S. Shivaji: strain CMS56

doi: 10.13145/bacdive8198.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Gammaproteobacteria
  • order: Pseudomonadales
  • family: Moraxellaceae
  • genus: Psychrobacter
  • species: Psychrobacter aquaticus
  • full scientific name: Psychrobacter aquaticus Shivaji et al. 2005

@ref: 5854

domain: Bacteria

phylum: Proteobacteria

class: Gammaproteobacteria

order: Pseudomonadales

family: Moraxellaceae

genus: Psychrobacter

species: Psychrobacter aquaticus

full scientific name: Psychrobacter aquaticus Shivaji et al. 2005

type strain: yes

Morphology

cell morphology

@refgram staincell shapemotility
30176negativecoccus-shapedno
26525coccus-shapedno
116437negativecoccus-shapedno

colony morphology

  • @ref: 26525
  • colony size: 2-3 mm
  • colony shape: circular
  • medium used: ZoBell Marine 2296 agar

pigmentation

  • @ref: 30176
  • production: no

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
5854OXOID NUTRIENT BROTH (DSMZ Medium 948)yeshttps://mediadive.dsmz.de/medium/948Name: OXOID NUTRIENT BROTH (DSMZ Medium 948) Composition: Nutrient broth 13.0 g/l Distilled water
5854NUTRIENT AGAR (OXOID CM3) (DSMZ Medium 605)yeshttps://mediadive.dsmz.de/medium/605Name: NUTRIENT AGAR (OXOID CM3) (DSMZ Medium 605) Composition: Agar 15.0 g/l NaCl 5.0 g/l Peptone 5.0 g/l Yeast extract 2.0 g/l Lab-Lemco beef extract 1.0 g/l Distilled water
5854LB (Luria-Bertani) MEDIUM (DSMZ Medium 381)yeshttps://mediadive.dsmz.de/medium/381Name: LB (Luria-Bertani) MEDIUM (DSMZ Medium 381) Composition: Agar 20.0 g/l NaCl 10.0 g/l Tryptone 10.0 g/l Yeast extract 5.0 g/l Distilled water
38181MEDIUM 72- for trypto casein soja agaryesDistilled water make up to (1000.000 ml);Trypto casein soy agar (40.000 g)
26525ZoBell Marine 2296 agaryes
116437CIP Medium 368yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=368
116437CIP Medium 72yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=72

culture temp

@refgrowthtypetemperaturerange
5854positivegrowth22psychrophilic
30176positivegrowth04-30
30176positiveoptimum22psychrophilic
38181positivegrowth22psychrophilic
26525positiveoptimum22psychrophilic
26525positivegrowth4-30
116437positivegrowth5-30
116437nogrowth37mesophilic
116437nogrowth41thermophilic

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
26525aerobe
116437obligate aerobe

halophily

@refsaltgrowthtested relationconcentration
30176NaClpositivegrowth0-10 %
30176NaClpositiveoptimum5 %
26525NaClpositivegrowth0-10 %
116437NaClpositivegrowth0-10 %

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
3017616449alanine+carbon source
3017622653asparagine+carbon source
3017629987glutamate+carbon source
3017617754glycerol+carbon source
3017637684mannose+carbon source
3017628044phenylalanine+carbon source
3017627082trehalose+carbon source
3017617632nitrate+reduction
2652530089acetate-carbon source
2652515963ribitol-carbon source
2652529016arginine-hydrolysis
2652528885butanol-carbon source
2652562968cellulose-carbon source
2652562968cellulose-degradation
2652516947citrate-carbon source
2652515824D-fructose-carbon source
2652517634D-glucose-builds acid from
2652517306maltose-carbon source
2652516899D-mannitol-carbon source
2652516024D-mannose-carbon source
2652516024D-mannose+builds acid from
2652528053melibiose-carbon source
2652516988D-ribose-carbon source
2652565327D-xylose-carbon source
2652552071dextran-carbon source
2652516813galactitol-carbon source
2652517113erythritol-carbon source
265254853esculin-hydrolysis
2652529806fumarate-carbon source
265255291gelatin-hydrolysis
2652517234glucose-fermentation
2652517234glucose-carbon source
2652517754glycerol+carbon source
2652545296hexadecane-carbon source
2652515443inulin-carbon source
26525L-alanine 4-nitroanilide+carbon source
2652530849L-arabinose-carbon source
2652530849L-arabinose-builds acid from
2652516467L-arginine-carbon source
2652517196L-asparagine-carbon source
2652517196L-asparagine+carbon source
2652517561L-cysteine-carbon source
2652529985L-glutamate+carbon source
2652518050L-glutamine-carbon source
2652515971L-histidine-carbon source
2652517191L-isoleucine-carbon source
2652515603L-leucine-carbon source
2652516643L-methionine-carbon source
2652517295L-phenylalanine+carbon source
2652517115L-serine-carbon source
2652516828L-tryptophan-carbon source
2652517895L-tyrosine-carbon source
2652517895L-tyrosine+carbon source
2652516414L-valine-carbon source
2652524996lactate-carbon source
2652517716lactose-carbon source
2652517716lactose-builds acid from
2652517790methanol-carbon source
2652517268myo-inositol-carbon source
2652517632nitrate+reduction
2652515361pyruvate-carbon source
2652530911sorbitol-carbon source
2652528017starch-hydrolysis
2652517992sucrose-carbon source
2652517992sucrose-builds acid from
2652526986threonine-carbon source
2652527082trehalose-carbon source
2652515318xanthine-carbon source
4340017057cellobiose-degradation
4340062968cellulose-degradation
4340017634D-glucose+assimilation
4340062345L-rhamnose+assimilation
4340017306maltose+assimilation
4340061993maltotriose+assimilation
68371Potassium 2-ketogluconate-builds acid from
6837124265gluconate-builds acid from
6837118403L-arabitol-builds acid from
6837118333D-arabitol-builds acid from
6837118287L-fucose-builds acid from
6837116443D-tagatose-builds acid from
6837162318D-lyxose-builds acid from
6837132528turanose-builds acid from
6837117151xylitol-builds acid from
6837128087glycogen-builds acid from
6837128017starch-builds acid from
6837116634raffinose-builds acid from
683716731melezitose-builds acid from
6837115443inulin-builds acid from
6837127082trehalose-builds acid from
6837117992sucrose-builds acid from
6837117306maltose-builds acid from
6837117814salicin-builds acid from
683714853esculin-builds acid from
6837118305arbutin-builds acid from
6837127613amygdalin-builds acid from
6837159640N-acetylglucosamine-builds acid from
68371320061methyl alpha-D-glucopyranoside-builds acid from
6837143943methyl alpha-D-mannoside-builds acid from
6837117924D-sorbitol-builds acid from
6837116899D-mannitol-builds acid from
6837117268myo-inositol-builds acid from
6837116813galactitol-builds acid from
6837162345L-rhamnose-builds acid from
6837117266L-sorbose-builds acid from
6837115824D-fructose-builds acid from
6837174863methyl beta-D-xylopyranoside-builds acid from
6837115963ribitol-builds acid from
6837165328L-xylose-builds acid from
6837117108D-arabinose-builds acid from
6837117113erythritol-builds acid from
6837117754glycerol-builds acid from
11643716947citrate-carbon source
1164374853esculin-hydrolysis
11643717632nitrate+reduction
11643716301nitrite-reduction
11643717632nitrate-respiration

antibiotic resistance

@refChEBImetaboliteis antibioticis sensitivesensitivity conc.is resistantresistance conc.
2652571415nitrofurantoinyesyes300 µg
265258309polymyxin byesyes300 µg
265252676amoxicillinyesyes100 µg
265257660nystatinyesyes100 µg
265253393carbenicillinyesyes50 µg
2652517698chloramphenicolyesyes30 µg
26525100147nalidixic acidyesyes30 µg
2652527902tetracyclineyesyes30 µg
265253770co-trimoxazoleyesyes25 µg
2652528864tobramycinyesyes20 µg
2652548923erythromycinyesyes15 µg
2652528971ampicillinyesyes10 µg
2652528669bacitracinyesyes10 µg
2652517833gentamicinyesyes10 µg
2652517076streptomycinyesyes10 µg
2652528077rifampicinyesyes5 µg
2652537943colistinyesyes10 µg
265256472lincomycinyesyes2 µg

metabolite production

@refChebi-IDmetaboliteproduction
2652515688acetoinno
2652516136hydrogen sulfideno
2652535581indoleno
11643735581indoleno

metabolite tests

@refChebi-IDmetabolitemethylred-testvoges-proskauer-testcitrate test
2652517234glucose-
2652515688acetoin-
2652516947citrate-
11643715688acetoin-
11643717234glucose-

enzymes

@refvalueactivityec
30176alkaline phosphatase+3.1.3.1
30176catalase+1.11.1.6
30176cytochrome oxidase+1.9.3.1
30176urease+3.5.1.5
26525catalase+1.11.1.6
26525cytochrome oxidase+1.9.3.1
26525lipase+
26525phosphatase+
26525arginine decarboxylase-4.1.1.19
26525lysine decarboxylase-4.1.1.18
26525urease+3.5.1.5
26525beta-galactosidase-3.2.1.23
116437oxidase+
116437beta-galactosidase-3.2.1.23
116437alcohol dehydrogenase-1.1.1.1
116437gelatinase-
116437amylase-
116437DNase-
116437caseinase-3.4.21.50
116437catalase+1.11.1.6
116437tween esterase+
116437gamma-glutamyltransferase+2.3.2.2
116437lecithinase-
116437lipase-
116437lysine decarboxylase-4.1.1.18
116437ornithine decarboxylase-4.1.1.17
116437phenylalanine ammonia-lyase-4.3.1.24
116437tryptophan deaminase-
116437urease+3.5.1.5
68382alkaline phosphatase+3.1.3.1
68382esterase (C 4)+
68382esterase lipase (C 8)+
68382lipase (C 14)+
68382leucine arylamidase+3.4.11.1
68382valine arylamidase-
68382cystine arylamidase-3.4.11.3
68382trypsin-3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase-3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase-3.2.1.20
68382beta-glucosidase-3.2.1.21
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
116437-+++++----++--------

API 50CHac

@refGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
116437---+/-+/-+/----+/-+/--+/--------------+/--+/-+/---------+/----+/------+/-

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
116437----+---------------++---------------------------+++-+----------------+--+----++++-+----++++++++-++

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcontinentcountryorigin.country
5854cyanobacterial mat sampleMcMurdoAustralia and Oceania
26525cyanobacteral mat samples from lake canopusMcmurdo Dry Valley RegionAustralia and OceaniaAntarcticaATA
116437Cyanobacterial material sampleAntarcticaAntarcticaATA

isolation source categories

Cat1Cat2Cat3
#Environmental#Microbial community#Microbial mat
#Host#Microbial#Bacteria
#Climate#Cold
#Condition#Psychrophilic (<10°C)
#Environmental#Aquatic#Lake (large)

taxonmaps

  • @ref: 69479
  • File name: preview.99_4732.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_80;96_193;97_209;98_234;99_4732&stattab=map
  • Last taxonomy: Psychrobacter
  • 16S sequence: AJ584833
  • Sequence Identity:
  • Total samples: 1363
  • soil counts: 114
  • aquatic counts: 834
  • animal counts: 378
  • plant counts: 37

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
58541Risk group (German classification)
1164371Risk group (French classification)

Sequence information

16S sequences

  • @ref: 30176
  • description: Psychrobacter aquatica partial 16S rRNA gene, isolate CMS 56
  • accession: AJ584833
  • length: 1481
  • database: nuccore
  • NCBI tax ID: 1354303

GC content

@refGC-content
585443
2652543.6

External links

@ref: 5854

culture collection no.: DSM 15339, MTCC 4386, CMS 56, CIP 108799

straininfo link

  • @ref: 77601
  • straininfo: 138035

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny15774658Psychrobacter vallis sp. nov. and Psychrobacter aquaticus sp. nov., from Antarctica.Shivaji S, Reddy GSN, Suresh K, Gupta P, Chintalapati S, Schumann P, Stackebrandt E, Matsumoto GIInt J Syst Evol Microbiol10.1099/ijs.0.03030-02005Antarctic Regions, Cyanobacteria/growth & development, DNA, Bacterial/analysis, DNA, Ribosomal/analysis, Fatty Acids/analysis, Fresh Water/*microbiology, Genes, rRNA, Geologic Sediments/*microbiology, Molecular Sequence Data, Phenotype, Phylogeny, Psychrobacter/chemistry/*classification/genetics/isolation & purification, RNA, Ribosomal, 16S/geneticsGenetics
Genetics24201199Draft Genome Sequence of Psychrobacter aquaticus Strain CMS 56T, Isolated from a Cyanobacterial Mat Sample Collected from Water Bodies in the McMurdo Dry Valley Region of Antarctica.Reddy GS, Ara S, Singh A, Kumar Pinnaka A, Shivaji SGenome Announc10.1128/genomeA.00918-132013Phylogeny

Reference

@idauthorscataloguedoi/urltitlepubmedID_cross_referencejournal
5854Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 15339)https://www.dsmz.de/collection/catalogue/details/culture/DSM-15339
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
2652510.1099/ijs.0.03030-015774658
30176Barberan A, Caceres Velazquez H, Jones S, Fierer N.10.1128/mSphere.00237-17Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information2877604126525
38181Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/6468
43400Stephan Hetzler, Daniel Bröker, Alexander Steinbüchel10.1128/AEM.01214-13Saccharification of Cellulose by Recombinant Rhodococcus opacus PD630 Strains23793636IJSEM 79: 5159-5166 2013
68371Automatically annotated from API 50CH acid
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
77601Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID138035.1StrainInfo: A central database for resolving microbial strain identifiers
116437Curators of the CIPCollection of Institut Pasteur (CIP 108799)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20108799