Name: Acinetobacter venetianus RAG-1
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 8144

Type strain:

Species: Acinetobacter venetianus

Strain Designation: RAG-1, Vaneechoutte ACI 403

Culture col. no.: KCTC 2702, BCRC 14357, DSM 23050, ATCC 31012, CCUG 45561, LMG 19082, LUH 3904, NIPH 1925, CIP 110063

Strain history: CIP <- 2009, M. Vaneechoutte, Ghent Univ. Hosp., Ghent, Belgium: strain RAG-1

NCBI tax ID(s): 1191460 (strain), 52133 (species)

SI-ID 46716

ATCC 31012, KCTC 2702, CCUG 45561, BCRC 14357, CCRC 14357, LMG19082, CIP 110063

Other strains from Acinetobacter venetianus

A. venetianus CCUG 60049, NRRL B-59471

Section

Acinetobacter venetianus RAG-1 is an aerobe, mesophilic, Gram-negative bacterium that was isolated from seawater.

Gram-negative
ovoid-shaped
aerobe
mesophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Pseudomonadota*
	Class	*Gammaproteobacteria*
	Order	*Pseudomonadales*
	Family	*Moraxellaceae*
	Genus	*Acinetobacter*
	Species	**Acinetobacter venetianus**
	Full Scientific Name (LPSN)	Acinetobacter venetianus (ex Di Cello et al. 1997) Vaneechoutte et al. 2009

Synonym

"Acinetobacter venetianus"

Information on morphological and physiological properties Morphology

[Ref.: #29005]	Gram stain	negative
[Ref.: #123813]	Gram stain	negative

[Ref.: #29005]	Cell shape	ovoid-shaped
[Ref.: #123813]	Cell shape	oval-shaped

[Ref.: #29005]	Motility	no
[Ref.: #123813]	Motility	no

[Ref.: #16671]	Type of hemolysis	alpha/beta
[Ref.: #16671]	Hemolysis ability	1

Information on culture and growth conditions Culture and growth conditions

[Ref.: #16671]

Culture medium

COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)

[Ref.: #16671]

Culture medium growth

[Ref.: #16671]

Culture medium link

Medium recipe at MediaDive

[Ref.: #16671]

Culture medium composition

expand / minimize

[Ref.: #16671]

Culture medium

TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92)

[Ref.: #16671]

Culture medium growth

[Ref.: #16671]

Culture medium link

Medium recipe at MediaDive

[Ref.: #16671]

Culture medium composition

expand / minimize

[Ref.: #42138]

Culture medium

MEDIUM 72- for trypto casein soja agar

[Ref.: #42138]

Culture medium growth

[Ref.: #42138]

Culture medium composition

expand / minimize

[Ref.: #123813]

Culture medium

CIP Medium 328

[Ref.: #123813]

Culture medium growth

[Ref.: #123813]

Culture medium link

Medium recipe provided by DSMZ

[Ref.: #123813]

Culture medium

CIP Medium 72

[Ref.: #123813]

Culture medium growth

[Ref.: #123813]

Culture medium link

Medium recipe provided by DSMZ

		Temperature
[Ref.: #16671]	growth	28 °C
[Ref.: #29005]	growth	30-37 °C
[Ref.: #29005]	optimum	33.5 °C
[Ref.: #42138]	growth	30 °C
[Ref.: #56822]	growth	30 °C

[Ref.: #16671]	Temperature range	mesophilic
[Ref.: #29005]	Temperature range	mesophilic
[Ref.: #42138]	Temperature range	mesophilic
[Ref.: #56822]	Temperature range	mesophilic

Information on physiology and metabolism Physiology and metabolism

[Ref.: #29005]	Oxygen tolerance	aerobe
[Ref.: #123813]	Oxygen tolerance	obligate aerobe

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #29005]	acetate ChEBI	+	carbon source
[Ref.: #68369]	adipate ChEBI	-	assimilation	* from API 20NE
[Ref.: #29005]	arginine ChEBI	+	carbon source
[Ref.: #68369]	arginine ChEBI	-	hydrolysis	* from API 20NE
[Ref.: #29005]	citrate ChEBI	+	carbon source
[Ref.: #68369]	D-glucose ChEBI	-	assimilation	* from API 20NE
[Ref.: #68369]	D-glucose ChEBI	-	fermentation	* from API 20NE
[Ref.: #68369]	D-mannitol ChEBI	-	assimilation	* from API 20NE
[Ref.: #68369]	D-mannose ChEBI	-	assimilation	* from API 20NE
[Ref.: #68369]	decanoate ChEBI	+	assimilation	* from API 20NE
[Ref.: #68369]	esculin ChEBI	-	hydrolysis	* from API 20NE
[Ref.: #29005]	ethanol ChEBI	+	carbon source
[Ref.: #68369]	gelatin ChEBI	+	hydrolysis	* from API 20NE
[Ref.: #68369]	gluconate ChEBI	-	assimilation	* from API 20NE
[Ref.: #29005]	histidine ChEBI	+	carbon source
[Ref.: #68369]	L-arabinose ChEBI	-	assimilation	* from API 20NE
[Ref.: #29005]	leucine ChEBI	+	carbon source
[Ref.: #29005]	malate ChEBI	+	carbon source
[Ref.: #68369]	malate ChEBI	+	assimilation	* from API 20NE
[Ref.: #29005]	malonate ChEBI	+	carbon source
[Ref.: #68369]	maltose ChEBI	-	assimilation	* from API 20NE
[Ref.: #68369]	N-acetylglucosamine ChEBI	-	assimilation	* from API 20NE
[Ref.: #123813]	nitrate ChEBI	+	reduction
[Ref.: #123813]	nitrite ChEBI	-	reduction
[Ref.: #68369]	tryptophan ChEBI	-	energy source	* from API 20NE
[Ref.: #68369]	urea ChEBI	-	hydrolysis	* from API 20NE
	Only first 10 entries are displayed. Click here to see all.

	Metabolite production	Metabolite	Production
[Ref.: #123813]		indole ChEBI	no
[Ref.: #68369]		indole ChEBI	no	* from API 20NE

	Physiological tests	Metabolite	Indole test
[Ref.: #68369]		indole ChEBI	-	* from API 20NE

	Enzyme	Enzyme activity	EC number
[Ref.: #68382]	acid phosphatase	-	3.1.3.2	* from API zym
[Ref.: #68382]	alkaline phosphatase	-	3.1.3.1	* from API zym
[Ref.: #68382]	alpha-chymotrypsin	-	3.4.21.1	* from API zym
[Ref.: #68382]	alpha-fucosidase	-	3.2.1.51	* from API zym
[Ref.: #68382]	alpha-galactosidase	-	3.2.1.22	* from API zym
[Ref.: #68382]	alpha-glucosidase	-	3.2.1.20	* from API zym
[Ref.: #68382]	alpha-mannosidase	-	3.2.1.24	* from API zym
[Ref.: #68369]	arginine dihydrolase	-	3.5.3.6	* from API 20NE
[Ref.: #68382]	beta-galactosidase	-	3.2.1.23	* from API zym
[Ref.: #68369]	beta-glucosidase	-	3.2.1.21	* from API 20NE, API zym
[Ref.: #68382]	beta-glucuronidase	-	3.2.1.31	* from API zym
[Ref.: #16671]	catalase	+	1.11.1.6
[Ref.: #123813]	catalase	+	1.11.1.6
[Ref.: #68382]	cystine arylamidase	+	3.4.11.3	* from API zym
[Ref.: #68369]	cytochrome oxidase	-	1.9.3.1	* from API 20NE
[Ref.: #16671]	cytochrome-c oxidase	-	1.9.3.1
[Ref.: #68382]	esterase (C 4)	+		* from API zym
[Ref.: #68382]	esterase lipase (C 8)	+		* from API zym
[Ref.: #68369]	gelatinase	+		* from API 20NE
[Ref.: #68382]	leucine arylamidase	+	3.4.11.1	* from API zym
[Ref.: #68382]	lipase (C 14)	+		* from API zym
[Ref.: #68382]	N-acetyl-beta-glucosaminidase	-	3.2.1.52	* from API zym
[Ref.: #68382]	naphthol-AS-BI-phosphohydrolase	-		* from API zym
[Ref.: #123813]	oxidase	-
[Ref.: #68382]	trypsin	-	3.4.21.4	* from API zym
[Ref.: #123813]	urease	-	3.5.1.5
[Ref.: #68369]	urease	-	3.5.1.5	* from API 20NE
[Ref.: #68382]	valine arylamidase	-		* from API zym
	Only first 10 entries are displayed. Click here to see all.

API® 20NE:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Reduction of nitratesNO3	Indole productionTRP	Fermentation of D-glucoseGLU_ Ferm	Arginine dihydrolaseADH (Arg)	Urease/urea hydrolysisURE	Esculin hydrolysis/beta-GlucosidaseESC	Gelatinase/gelatin hydrolysisGEL	beta-GalactosidasePNPG	Assimilation of D-glucoseGLU_ Assim	Assimilation of L-arabinoseARA	Assimilation of D-mannoseMNE	Assimilation of D-mannitolMAN	Assimilation of N-acetyl-glucosamineNAG	Assimilation of maltoseMAL	Assimilation of gluconateGNT	Assimilation of capric acidCAP	Assimilation adipic acidADI	Assimilation of malic acidMLT	Assimilation of citric acidCIT	Assimilation of phenyl-acetic acidPAC	Cytochrome oxidaseOX
[Ref.: #16671]	24822	+	-	-	-	-	-	+	-	-	-	-	-	-	-	-	+	-	+	+	-	-
[Ref.: #16671]	23855	-	-	-	-	-	-	+	-	-	-	-	-	-	-	-	+	-	+	+	-	-

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #123813]	12337	-	-	+	+	+	+	-	+	-	-	-	-	-	-	-	-	-	-	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #16671]	Sample type/isolated from	seawater
[Ref.: #16671]	Geographic location (country and/or sea, region)	Tel Baruch
[Ref.: #16671]	Country	Israel
[Ref.: #16671]	Country ISO 3 Code	ISR
[Ref.: #16671]	Continent	Asia

[Ref.: #56822]	Sample type/isolated from	Tar on beach
[Ref.: #56822]	Geographic location (country and/or sea, region)	Israelian beach (Tel Baruch)

[Ref.: #123813]	Sample type/isolated from	Environment, Sea water, beach
[Ref.: #123813]	Geographic location (country and/or sea, region)	Tel Baruch
[Ref.: #123813]	Country	Israel
[Ref.: #123813]	Country ISO 3 Code	ISR
[Ref.: #123813]	Continent	Asia
* marker position based on {}

Isolation sources categories

#Environmental

#Aquatic

#Marine

What are isolation sources categories?

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #16671]	Biosafety level	1	Risk group (German classification) According to TRBA 466
[Ref.: #123813]	Biosafety level	1	Risk group (French classification)

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #16671]	Acinetobacter venetianus 16S rRNA gene, strain ATCC 31012	AJ295007	1463	ENA	1191460 tax ID
[Ref.: #29005]	Acinetobacter venetianus partial 16S rRNA gene, strain ACI555	AM909651	1410	GenBank	52133 tax ID
[Ref.: #20218]	Acinetobacter venetianus strain CCUG 45561 16S-23S ribosomal RNA intergenic spacer, complete sequence	AY601846	612	ENA	52133 tax ID	*
[Ref.: #20218]	Acinetobacter sp. ATCC 31012 16S ribosomal RNA gene, partial sequence	AF542963	1456	ENA	1191460 tax ID	*

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Acinetobacter venetianus RAG-1	2565956684	draft	JGI IMG/M	1191460 tax ID	*
[Ref.: #66792]	Acinetobacter venetianus RAG-1	2551306081	draft	JGI IMG/M	1191460 tax ID	*

[Ref.: #16671]

GC-content

43.9 mol%

Availability in culture collections External links

[Ref.: #16671]

Culture collection no.

KCTC 2702, BCRC 14357, DSM 23050, ATCC 31012, CCUG 45561, LMG 19082, LUH 3904, NIPH 1925, CIP 110063

[Ref.: #77548]

SI-ID 46716

Literature:

Only first 10 entries are displayed. Click here to see all.

Topic	Title	Authors	Journal	DOI	Year
Metabolism	Enhanced degradation of different crude oils by defined engineered consortia of Acinetobacter venetianus RAG-1 mutants based on their alkane metabolism.	Liu J, Zhao B, Lan Y, Ma T	Bioresour Technol	10.1016/j.biortech.2021.124787	2021	*
Metabolism	Transcriptomic analysis of the highly efficient oil-degrading bacterium Acinetobacter venetianus RAG-1 reveals genes important in dodecane uptake and utilization.	Kothari A, Charrier M, Wu YW, Malfatti S, Zhou CE, Singer SW, Dugan L, Mukhopadhyay A	FEMS Microbiol Lett	10.1093/femsle/fnw224	2016	*
Phylogeny	Genomic and phenotypic characterization of the species Acinetobacter venetianus.	Fondi M, Maida I, Perrin E, Orlandini V, La Torre L, Bosi E, Negroni A, Zanaroli G, Fava F, Decorosi F, Giovannetti L, Viti C, Vaneechoutte M, Dijkshoorn L, Fani R	Sci Rep	10.1038/srep21985	2016	*
Genetics	Draft genome sequence of the hydrocarbon-degrading and emulsan-producing strain Acinetobacter venetianus RAG-1T.	Fondi M, Orlandini V, Emiliani G, Papaleo MC, Maida I, Perrin E, Vaneechoutte M, Dijkshoorn L, Fani R	J Bacteriol	10.1128/JB.01019-12	2012	*
Metabolism	Molecular and phenotypic characterization of Acinetobacter strains able to degrade diesel fuel.	Mara K, Decorosi F, Viti C, Giovannetti L, Papaleo MC, Maida I, Perrin E, Fondi M, Vaneechoutte M, Nemec A, van den Barselaar M, Dijkshoorn L, Fani R	Res Microbiol	10.1016/j.resmic.2011.12.002	2011	*
Phylogeny	Description of Acinetobacter venetianus ex Di Cello et al. 1997 sp. nov.	Vaneechoutte M, Nemec A, Musilek M, van der Reijden TJ, van den Barselaar M, Tjernberg I, Calame W, Fani R, De Baere T, Dijkshoorn L	Int J Syst Evol Microbiol	10.1099/ijs.0.003541-0	2009	*
Metabolism	Optimizing emulsan production of A. venetianus RAG-1 using response surface methodology.	Su WT, Chen WJ, Lin YF	Appl Microbiol Biotechnol	10.1007/s00253-009-1957-0	2009	*
Enzymology	Influence of deletions in the apoemulsan gene cluster on A. venetianus RAG-1 polysaccharide biosynthesis.	Dams-Kozlowska H, Mercaldi MP, Ramjeawan A, Kaplan DL	J Microbiol Biotechnol	8150	2008	*
Metabolism	Modifications and applications of the Acinetobacter venetianus RAG-1 exopolysaccharide, the emulsan complex and its components.	Dams-Kozlowska H, Mercaldi MP, Panilaitis BJ, Kaplan DL	Appl Microbiol Biotechnol	10.1007/s00253-008-1664-2	2008	*
Metabolism	Hydrophobic bacteria at the hexadecane-water interface: examination of micrometre-scale interfacial properties.	Kang Z, Yeung A, Foght JM, Gray MR	Colloids Surf B Biointerfaces	10.1016/j.colsurfb.2008.07.015	2008	*
Enzymology	Transesterification activity of a novel lipase from Acinetobacter venetianus RAG-1.	Snellman EA, Colwell RR	Antonie Van Leeuwenhoek	10.1007/s10482-008-9276-5	2008	*
Phylogeny	Discovery of the dual polysaccharide composition of emulsan and the isolation of the emulsion stabilizing component.	Mercaldi MP, Dams-Kozlowska H, Panilaitis B, Joyce AP, Kaplan DL	Biomacromolecules	10.1021/bm800239p	2008	*
Metabolism	Construction of a chimeric gene cluster for the biosynthesis of apoemulsan with altered molecular weight.	Dams-Kozlowska H, Sainath N, Kaplan DL	Appl Microbiol Biotechnol	10.1007/s00253-008-1346-0	2008	*
Metabolism	Biosynthesis of emulsan biopolymers from agro-based feedstocks.	Panilaitis B, Castro GR, Solaiman D, Kaplan DL	J Appl Microbiol	10.1111/j.1365-2672.2006.03078.x	2007	*
Enzymology	A unique polypeptide from the C-terminus of the exocellular esterase of Acinetobacter venetianus RAG-1 modulates the emulsifying activity of the polymeric bioemulsifier apoemulsan.	Bach H, Gutnick DL	Appl Microbiol Biotechnol	10.1007/s00253-005-0161-0	2005	*
Metabolism	Novel polysaccharide-protein-based amphipathic formulations.	Bach H, Gutnick DL	Appl Microbiol Biotechnol	10.1007/s00253-005-0149-9	2005	*
Metabolism	Emulsan quantitation by Nile red quenching fluorescence assay.	Castro GR, Larson BK, Panilaitis B, Kaplan DL	Appl Microbiol Biotechnol	10.1007/s00253-004-1849-2	2004	*
Metabolism	The concentrations of hexadecane and inorganic nutrients modulate the production of extracellular membrane-bound vesicles, soluble protein, and bioemulsifier by Acinetobacter venetianus RAG-1 and Acinetobacter sp. strain HO1-N.	Leahy JG, Khalid ZM, Quintero EJ, Jones-Meehan JM, Heidelberg JF, Batchelor PJ, Colwell RR	Can J Microbiol	10.1139/w03-071	2003	*
Enzymology	An exocellular protein from the oil-degrading microbe Acinetobacter venetianus RAG-1 enhances the emulsifying activity of the polymeric bioemulsifier emulsan.	Bach H, Berdichevsky Y, Gutnick D	Appl Environ Microbiol	10.1128/AEM.69.5.2608-2615.2003	2003	*
Phylogeny	Oil-degrading Acinetobacter strain RAG-1 and strains described as 'Acinetobacter venetianus sp. nov.' belong to the same genomic species.	Vaneechoutte M, Tjernberg I, Baldi F, Pepi M, Fani R, Sullivan ER, van der Toorn J, Dijkshoorn L	Res Microbiol	10.1016/s0923-2508(99)80047-3	1999	*
Metabolism	A Novel FadL Homolog, AltL, Mediates Transport of Long-Chain Alkanes and Fatty Acids in Acinetobacter venetianus RAG-1.	Liu J, Chen S, Zhao B, Li G, Ma T	Appl Environ Microbiol	10.1128/aem.01294-22	2022	*
	Analysis of force interactions between AFM tips and hydrophobic bacteria using DLVO theory.	Dorobantu LS, Bhattacharjee S, Foght JM, Gray MR	Langmuir	10.1021/la9001237	2009	*
	Atomic force microscopy measurement of heterogeneity in bacterial surface hydrophobicity.	Dorobantu LS, Bhattacharjee S, Foght JM, Gray MR	Langmuir	10.1021/la7035295	2008	*
	Mechanical properties of hexadecane-water interfaces with adsorbed hydrophobic bacteria.	Kang Z, Yeung A, Foght JM, Gray MR	Colloids Surf B Biointerfaces	10.1016/j.colsurfb.2007.10.021	2007	*
	Adhesion of acinetobacter venetianus to diesel fuel droplets studied with In situ electrochemical and molecular probes	Baldi F, Ivosevic N, Minacci A, Pepi M, Fani R, Svetlicic V V, utic V V	Appl Environ Microbiol	10.1128/AEM.65.5.2041-2048.1999	1999	*

References

#16671

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 23050

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#20218

Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )

#29005

Barberan A, Caceres Velazquez H, Jones S, Fierer N.: Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information. mSphere 2: 2017 ( DOI 10.1128/mSphere.00237-17 , PubMed 28776041 ) - originally annotated from #25438 (see below)

#42138 ; Curators of the CIP;
#56822 Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 45561

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#68369

Automatically annotated from API 20NE .

#68382

Automatically annotated from API zym .

#77548

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID46716.1 )

#123813 Collection of Institut Pasteur ; Curators of the CIP; CIP 110063

#25438

IJSEM 1376 2009 ( DOI 10.1099/ijs.0.003541-0 , PubMed 19502319 )

* These data were automatically processed and therefore are not curated

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