Acinetobacter calcoaceticus (DSM 1139, NCIB 9205, CCUG 3352)

Name: Acinetobacter calcoaceticus (DSM 1139, NCIB 9205, CCUG 3352)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 8054

Type strain

Strain Designation HO1-N

Culture col. no. DSM 1139 NCIB 9205 CCUG 3352 ATCC 14987 LMG 1056 3 more

NCBI tax ID(s) 471

Special Collections DSMZ CCUG CIP

History <- W. R. Finnerty, Univ. Georgia; HO1-N (Acinetobacter sp.) CIP <- 1966, L. Rey <- R.E. Kallio, Iowa, USA: strain HO-1, Micrococcus cerificans

Links

Acinetobacter calcoaceticus HO1-N is an obligate aerobe, Gram-negative, rod-shaped bacterium that was isolated from hexadecane enrichments.

Gram-negative rod-shaped obligate aerobe 16S sequence Bacteria

Name and taxonomic classification

SI-ID 92091

LMG 1056,ATCC 23236,CCUG 3352,DSM 1139,IFO 12552,NCIB 9205,ATCC 14987,ATCC 21662,NCIMB 9205,CIP 66.33,CIP 68.32,LMD 82.32,NCCB 82032,NBRC 12552,BCRC 11562,CCRC 11562,NCIM 2890

@ref 20215

Last LPSN update 2026-02-09 11:19:37

Domain Bacteria

Phylum Pseudomonadota

Class Gammaproteobacteria

Order Pseudomonadales

Family Moraxellaceae

Genus Acinetobacter

Species Acinetobacter calcoaceticus

Full scientific name Acinetobacter calcoaceticus (Beijerinck 1911) Baumann et al. 1968 (Approved Lists 1980)

Synonyms (1)

"Micrococcus calcoaceticus"

BacDive ID	Other strains from **Acinetobacter calcoaceticus** (17)	Type strain
8057	A. calcoaceticus DSM 30006, ATCC 23055, CIP 81.08, CIP 81.8, ... (type strain)
8055	A. calcoaceticus SW1, DSM 16962
8056	A. calcoaceticus IH9, DSM 19654
8111	A. calcoaceticus Tr III-761, DSM 9319
135231	A. calcoaceticus CIP 103625, ATCC 49137
135373	A. calcoaceticus CIP 110488, ANC 3680
135648	A. calcoaceticus CIP 66.32, ATCC 17902
137460	A. calcoaceticus CIP 110439, NIPH 13, CCM 4665
142792	A. calcoaceticus CCUG 14821
155464	A. calcoaceticus CCUG 57816, LMG 10517
157288	A. calcoaceticus IMI 347320
157583	A. calcoaceticus UHS000024, GK 2, GK2, DSM 106070
161716	A. calcoaceticus JCM 16087
161719	A. calcoaceticus JCM 16090
161720	A. calcoaceticus JCM 16091
161721	A. calcoaceticus JCM 16092
161722	A. calcoaceticus JCM 16095

Morphology

Cell morphology

@ref	Gram stain	Cell shape	Motility
117097	negative	rod-shaped

Colony morphology

Culture and growth conditions

Culture medium

@ref	Name	Medium link	Composition
32773	MEDIUM 72- for trypto casein soja agar		Distilled water make up to (1000.000 ml);Trypto casein soy agar (40.000 g)
577	NUTRIENT AGAR (DSMZ Medium 1)	Medium recipe at MediaDive	Name: NUTRIENT AGAR (DSMZ Medium 1) Composition: Agar 15.0 g/l Peptone 5.0 g/l Meat extract 3.0 g/l Distilled water
577	TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92)	Medium recipe at MediaDive	Name: TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Yeast extract 3.0 g/l Distilled water
577	COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)	Medium recipe at MediaDive	Name: COLUMBIA BLOOD MEDIUM (DSMZ Medium 693) Composition: Defibrinated sheep blood 50.0 g/l Columbia agar base
117097	CIP Medium 72	Medium recipe at CIP

Culture temp

@ref	Growth	Type	Temperature (°C)
577	positive	growth	30
32773	positive	growth	30
117097	positive	growth	10-41
117097	negative	growth	45

Physiology and metabolism

Oxygen tolerance

117097

Oxygen toleranceobligate aerobe

Halophily

@ref	Salt	Growth	Tested relation	Concentration
117097	NaCl	positive	growth	0-2 %
117097	NaCl		growth	4 %
117097	NaCl		growth	6 %
117097	NaCl		growth	8 %
117097	NaCl		growth	10 %

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
68369	17128 ChEBI	adipate	+	assimilation	from API 20NE
68369	29016 ChEBI	arginine	-	hydrolysis	from API 20NE
117097	16947 ChEBI	citrate	+	carbon source
68369	17634 ChEBI	D-glucose	-	assimilation	from API 20NE
68369	17634 ChEBI	D-glucose	+	fermentation	from API 20NE
68369	16899 ChEBI	D-mannitol	-	assimilation	from API 20NE
68369	16024 ChEBI	D-mannose	-	assimilation	from API 20NE
68369	27689 ChEBI	decanoate	+	assimilation	from API 20NE
68369	4853 ChEBI	esculin	-	hydrolysis	from API 20NE
68369	5291 ChEBI	gelatin	-	hydrolysis	from API 20NE
68369	24265 ChEBI	gluconate	-	assimilation	from API 20NE
117097	17234 ChEBI	glucose	+/-	degradation
68369	30849 ChEBI	L-arabinose	+	assimilation	from API 20NE
68369	25115 ChEBI	malate	+	assimilation	from API 20NE
68369	17306 ChEBI	maltose	-	assimilation	from API 20NE
68369	59640 ChEBI	N-acetylglucosamine	-	assimilation	from API 20NE
117097	17632 ChEBI	nitrate	-	reduction
68369	17632 ChEBI	nitrate	-	reduction	from API 20NE
117097	16301 ChEBI	nitrite	-	reduction
68369	27897 ChEBI	tryptophan	-	energy source	from API 20NE
68369	16199 ChEBI	urea	-	hydrolysis	from API 20NE

Antibiotic resistance

@ref	Metabolite	Is sensitive	Is resistant
117097	0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)

Metabolite production

@ref	Chebi-ID	Metabolite	Production
68369	35581 ChEBI	indole		from API 20NE

Metabolite tests

@ref	Chebi-ID	Metabolite	Indole test	Voges-proskauer-test	Methylred-test
117097	15688 ChEBI	acetoin		-
117097	17234 ChEBI	glucose			-
68369	35581 ChEBI	indole	-			from API 20NE

Enzymes

@ref	Value	Activity	Ec
68382	acid phosphatase	+	3.1.3.2	from API zym
68382	alkaline phosphatase	+	3.1.3.1	from API zym
68382	alpha-chymotrypsin	-	3.4.21.1	from API zym
68382	alpha-fucosidase	-	3.2.1.51	from API zym
68382	alpha-galactosidase	-	3.2.1.22	from API zym
68382	alpha-glucosidase	-	3.2.1.20	from API zym
68382	alpha-mannosidase	-	3.2.1.24	from API zym
68369	arginine dihydrolase	-	3.5.3.6	from API 20NE
68382	beta-galactosidase	-	3.2.1.23	from API zym
117097	beta-galactosidase	-	3.2.1.23
68382	beta-glucosidase	-	3.2.1.21	from API zym
68369	beta-glucosidase	-	3.2.1.21	from API 20NE
68382	beta-glucuronidase	-	3.2.1.31	from API zym
117097	catalase	+	1.11.1.6
68382	cystine arylamidase	-	3.4.11.3	from API zym
68369	cytochrome oxidase	-	1.9.3.1	from API 20NE
68382	esterase (C 4)	+		from API zym
68382	esterase lipase (C 8)	+		from API zym
117097	gamma-glutamyltransferase	+	2.3.2.2
117097	gelatinase	-
68369	gelatinase	-		from API 20NE
68382	leucine arylamidase	+	3.4.11.1	from API zym
68382	lipase (C 14)	+		from API zym
68382	N-acetyl-beta-glucosaminidase	-	3.2.1.52	from API zym
68382	naphthol-AS-BI-phosphohydrolase	+		from API zym
117097	oxidase	-
68382	trypsin	-	3.4.21.4	from API zym
117097	urease	-	3.5.1.5
68369	urease	-	3.5.1.5	from API 20NE
68382	valine arylamidase	-		from API zym

API zym

@ref	Control	Alkaline phosphatase	Esterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	Lipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	Trypsin	alpha- Chymotrypsin	Acid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	alpha- Galactosidase	beta- Galactosidase	beta- Glucuronidase	alpha- Glucosidase	beta- Glucosidase	N-acetyl-beta- glucosaminidase	alpha- Mannosidase	alpha- Fucosidase
117097	-	+	+	+	+	+	-	-	-	-	+	+	-	-	-	-	-	-	-	-

API 20NE

@ref	Reduction of nitratesNO3	TRP	GLU_ Ferm	ADH (Arg)	URE	ESC	GEL	PNPG	GLU_ Assim	ARA	MNE	MAN	NAG	MAL	GNT	CAP	ADI	MLT	CIT	PAC	OX
577	-	-	+	-	-	-	-	-	-	+	-	-	-	-	-	+	+	+	+	+	-

API biotype100

@ref	ControlQ	D-glucose as carbon sourceGLU	D-fructose as carbon sourceFRU	D-galactose as carbon sourceGAL	D-trehalose as carbon sourceTRE	D-mannose as carbon sourceMNE	L-sorbose as carbon sourceSBE	D-melibiose as carbon sourceMEL	sucrose as carbon sourceSAC	D-raffinose as carbon sourceRAF	maltotriose as carbon sourceMTE	maltose as carbon sourceMAL	lactose as carbon sourceLAC	lactulose as carbon sourceLTE	1-O-methyl-beta-galactopyranoside as carbon sourceMbGa	1-O-methyl-alpha-galactopyranoside as carbon sourceMaGa	D-cellobiose as carbon sourceCEL	gentiobiose as carbon sourceGEN	1-O-methyl-beta-D-glucopyranoside as carbon sourceMbGu	esculin as carbon sourceESC	D-ribose as carbon sourceRIB	L-arabinose as carbon sourceARA	D-xylose as carbon sourceXYL	palatinose as carbon sourcePLE	L-rhamnose as carbon sourceRHA	L-fucose as carbon sourceFUC	D-melezitose as carbon sourceMLZ	D-arabitol as carbon sourceDARL	L-arabitol as carbon sourceLARL	xylitol as carbon sourceXLT	dulcitol as carbon sourceDUL	D-tagatose as carbon sourceTAG	glycerol as carbon sourceGLY	myo-inositol as carbon sourceINO	D-mannitol as carbon sourceMAN	maltitol as carbon sourceMTL	D-turanose as carbon sourceTUR	D-sorbitol as carbon sourceSOR	adonitol as carbon sourceADO	hydroxyquinoline-beta-glucuronide as carbon sourceHBG	D-lyxose as carbon sourceLYX	i-erythritol as carbon sourceERY	1-O-methyl-alpha-D-glucoside as carbon sourceMDG	3-O-methyl-D-glucose as carbon source3MDG	D-saccharate as carbon sourceSAT	mucate as carbon sourceMUC	L-tartrate as carbon sourceLTAT	D-tartrate as carbon sourceDTAT	meso-tartrate as carbon sourceMTAT	D-malate as carbon sourceDMLT	L-malate as carbon sourceLMLT	cis-aconitate as carbon sourceCATE	trans-aconitate as carbon sourceTATE	tricarballylate as carbon sourceTTE	citrate as carbon sourceCIT	D-glucuronate as carbon sourceGRT	D-galacturonate as carbon sourceGAT	2-ketogluconate as carbon source2KG	5-ketogluconate as carbon source5KG	tryptophan as carbon sourceTRY	N-acetyl-D-glucosamine as carbon sourceNAG	D-gluconate as carbon sourceGNT	phenylacetate as carbon sourcePAC	protocatechuate as carbon sourcePAT	4-hydroxybenzoate as carbon sourcepOBE	quinate as carbon sourceQAT	gentisate as carbon sourceGTE	3-hydroxybenzoate as carbon sourcemOBE	benzoate as carbon sourceBAT	3-phenylpropionate as carbon sourcePPAT	m-coumarate as carbon sourceCMT	trigonelline as carbon sourceTGE	betaine as carbon sourceBET	putrescine as carbon sourcePCE	4-aminobutyrate as carbon sourceABT	histamine as carbon sourceHIN	DL-lactate as carbon sourceLAT	caprate as carbon sourceCAP	caprylate as carbon sourceCYT	L-histidine as carbon sourceHIS	succinate as carbon sourceSUC	fumarate as carbon sourceFUM	glutarate as carbon sourceGRE	DL-glycerate as carbon sourceGYT	5-aminovalerate as carbon sourceAVT	ethanolamine as carbon sourceETN	tryptamine as carbon sourceTTN	D-glucosamine as carbon sourceGLN	itaconate as carbon sourceITA	3-hydroxybutyrate as carbon source3OBU	L-aspartate as carbon sourceAPT	L-glutamate as carbon sourceGTT	L-proline as carbon sourcePRO	D-alanine as carbon sourceDALA	L-alanine as carbon sourceLALA	L-serine as carbon sourceSER	malonate as carbon sourceMNT	propionate as carbon sourcePROP	L-tyrosine as carbon sourceTYR	2-ketoglutarate as carbon source2KT
117097	not determinedn.d.	+	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	+	+	+	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	+	+	-	-	-	+	+	+	+	+	+	-	+	-	-	-	-	-	+	+	+	+	-	-	+	-	-	-	-	+	+	-	+	+	+	-	+	+	+	-	-	-	-	-	-	+	+	+	+	+	+	-	+	+	+	+

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Engineered	#Laboratory	#Lab enrichment

Isolation

@ref	Sample type	Geographic location	Country	Country ISO 3 Code	Continent
577	hexadecane enrichments
44646	Soil,enrichment with n-hexadecane	Iowa	USA	USA	North America
117097	Environment, Soil enriched with n-hexadecane	Iowa	United States of America	USA	North America

Taxonmaps

69479

Global distribution of 16S sequence X81657 (>99% sequence identity) for Acinetobacter from Microbeatlas

Aquatic Samples	12125
Soil Samples	7889
Animal Samples	20278
Plant Samples	5974
Total Samples	46266

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
577	2	Risk group (German classification) According to TRBA 466
117097	1	Risk group (French classification) According to TRBA 466

Sequence information

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
577	A.calcoaceticus 16S rRNA gene (DSM1139)	X81657	1460		471

Genome-based predictions

Literature

Topic	Title	Authors	Journal	DOI	Year
Enzymology	Comprehensive Laboratory Evaluation of a Specific Lateral Flow Assay for the Presumptive Identification of Francisella tularensis in Suspicious White Powders and Aerosol Samples.	Pillai SP, DePalma L, Prentice KW, Ramage JG, Chapman C, Sarwar J, Parameswaran N, Petersen J, Yockey B, Young J, Singh A, Pillai CA, Manickam G, Thirunavkkarasu N, Avila JR, Sharma S, Morse SA, Venkateswaran K, Anderson K, Hodge DR.	Health Secur	10.1089/hs.2019.0151	2020
Enzymology	Comprehensive Laboratory Evaluation of a Lateral Flow Assay for the Detection of Yersinia pestis.	Prentice KW, DePalma L, Ramage JG, Sarwar J, Parameswaran N, Petersen J, Yockey B, Young J, Joshi M, Thirunavvukarasu N, Singh A, Chapman C, Avila JR, Pillai CA, Manickam G, Sharma SK, Morse SA, Venkateswaran KV, Anderson K, Hodge DR, Pillai SP.	Health Secur	10.1089/hs.2019.0094	2019
Enzymology	Comprehensive Laboratory Evaluation of a Highly Specific Lateral Flow Assay for the Presumptive Identification of Bacillus anthracis Spores in Suspicious White Powders and Environmental Samples.	Ramage JG, Prentice KW, DePalma L, Venkateswaran KS, Chivukula S, Chapman C, Bell M, Datta S, Singh A, Hoffmaster A, Sarwar J, Parameswaran N, Joshi M, Thirunavkkarasu N, Krishnan V, Morse S, Avila JR, Sharma S, Estacio PL, Stanker L, Hodge DR, Pillai SP.	Health Secur	10.1089/hs.2016.0041	2016
Phylogeny	Development of a multilocus sequence typing scheme for characterization of clinical isolates of Acinetobacter baumannii.	Bartual SG, Seifert H, Hippler C, Luzon MA, Wisplinghoff H, Rodriguez-Valera F.	J Clin Microbiol	10.1128/jcm.43.9.4382-4390.2005	2005
	Efficient Synergistic Antibacterial Activity of alpha-MSH Using Chitosan-Based Versatile Nanoconjugates.	Barman S, Chakraborty A, Saha S, Sikder K, Maitra Roy S, Modi B, Bahadur S, Khan AH, Manna D, Bag P, Sarkar AK, Bhattacharya R, Basu A, Maity AR.	ACS Omega	10.1021/acsomega.2c08209	2023
	Development of a Colloidal Gold-Based Immunochromatographic Strip for Rapid Detection of Klebsiella pneumoniae Serotypes K1 and K2.	Siu LK, Tsai YK, Lin JC, Chen TL, Fung CP, Chang FY.	J Clin Microbiol	10.1128/jcm.01608-16	2016
Enzymology	Utility of peptide nucleic acid fluorescence in situ hybridization for rapid detection of Acinetobacter spp. and Pseudomonas aeruginosa.	Peleg AY, Tilahun Y, Fiandaca MJ, D'Agata EM, Venkataraman L, Moellering RC, Eliopoulos GM.	J Clin Microbiol	10.1128/jcm.01724-08	2009
Phylogeny	Species-level identification of isolates of the Acinetobacter calcoaceticus-Acinetobacter baumannii complex by sequence analysis of the 16S-23S rRNA gene spacer region.	Chang HC, Wei YF, Dijkshoorn L, Vaneechoutte M, Tang CT, Chang TC.	J Clin Microbiol	10.1128/jcm.43.4.1632-1639.2005	2005
	Extradiol cleavage of 3-methylcatechol by catechol 1,2-dioxygenase from various microorganisms.	Hou CT, Patel R, Lillard MO.	Appl Environ Microbiol	10.1128/aem.33.3.725-727.1977	1977
Pathogenicity	Defensins mediate the microbicidal activity of human neutrophil granule extract against Acinetobacter calcoaceticus.	Greenwald GI, Ganz T.	Infect Immun	10.1128/iai.55.6.1365-1368.1987	1987
Enzymology	Catechol 1,2-dioxygenase from Acinetobacter calcoaceticus: purification and properties.	Patel RN, Hou CT, Felix A, Lillard MO.	J Bacteriol	10.1128/jb.127.1.536-544.1976	1976
	Identification of Acinetobacter isolates in the A. calcoaceticus-A. baumannii complex by restriction analysis of the 16S-23S rRNA intergenic-spacer sequences.	Dolzani L, Tonin E, Lagatolla C, Prandin L, Monti-Bragadin C.	J Clin Microbiol	10.1128/jcm.33.5.1108-1113.1995	1995
	Designed Multifunctional Peptides for Intracellular Targets.	Juretic D.	Antibiotics (Basel)	10.3390/antibiotics11091196	2022
Enzymology	Purification and antimicrobial properties of three defensins from rat neutrophils.	Eisenhauer PB, Harwig SS, Szklarek D, Ganz T, Selsted ME, Lehrer RI.	Infect Immun	10.1128/iai.57.7.2021-2027.1989	1989
Phylogeny	Interspecies transformation of Acinetobacter: genetic evidence for a ubiquitous genus.	Juni E.	J Bacteriol	10.1128/jb.112.2.917-931.1972	1972
Enzymology	Long-chain alcohol and aldehyde dehydrogenase activities in Acinetobacter calcoaceticus strain HO1-N.	Fox MG, Dickinson FM, Ratledge C	J Gen Microbiol	10.1099/00221287-138-9-1963	1992
	Plasmid RP1-mediated susceptibility of Acinetobacter calcoaceticus to rat polymorphonuclear leukocyte granule contents.	Loeffelholz MJ, Modrzakowski MC	Infect Immun	10.1128/iai.54.3.705-709.1986	1986
Enzymology	Transformation and mobilization of cloning vectors in Acinetobacter spp.	Singer JT, van Tuijl JJ, Finnerty WR	J Bacteriol	10.1128/jb.165.1.301-303.1986	1986
Enzymology	Insertional specificity of transposon Tn5 in Acinetobacter sp.	Singer JT, Finnerty WR	J Bacteriol	10.1128/jb.157.2.607-611.1984	1984

References

#577	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 1139
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#32773	; Curators of the CIP;
#44646	Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 3352
#68369	Automatically annotated from API 20NE .
#68382	Automatically annotated from API zym .
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#117097	Collection of Institut Pasteur ; Curators of the CIP; CIP 66.33
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Acinetobacter calcoaceticus (DSM 1139, NCIB 9205, CCUG 3352)

Name and taxonomic classification

Morphology

Cell morphology

Colony morphology

Culture and growth conditions

Culture medium

Culture temp

Physiology and metabolism

Oxygen tolerance

Halophily

Metabolite utilization

Antibiotic resistance

Metabolite production

Metabolite tests

Enzymes

API zym

API 20NE

API biotype100

Isolation, sampling and environmental information

Isolation source categories

Isolation

Taxonmaps

Interaction and safety

Risk assessment

Sequence information

16S sequences

Genome-based predictions

Literature

Literature

References

BacDive

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