Name: Nesterenkonia halobia 28-3
Creator: BacDive
License: https://creativecommons.org/licenses/by/4.0/

Strain identifier

BacDive ID: 7762

Type strain:

Species: Nesterenkonia halobia

Strain Designation: 28-3

Culture col. no.: DSM 20541, ATCC 21727, CCM 2591, CCUG 38889, JCM 11483, BCRC 11656, CECT 5018, CGMCC 1.2323, CIP 81.68, IAM 13001, IFO 15353, IMET 11383, IMSNU 21325, KCTC 3518, LMG 14214, LMG 21452, MTCC 701, NBRC 15353, NCIMB 2204, VKM Ac-2256, VKM B-1317, VKM B-1421

Strain history: CIP <- 1981, CCM, Micrococcus halobius <- H. Onishi: strain 28-3

NCBI tax ID(s): 37922 (species)

SI-ID 9303

LMG 14214, ATCC 21727, CCM 2591, CCRC 11656, DSM 20541, IAM 13001, IFO 15353, NCIMB 2204, CCUG 38889, VKM B-1317, VKM B-1421, CIP 81.68, LMG 21452, CECT 5018, IMET 11383, JCM 11483, KCTC 3518, NBRC 15353, BCRC 11656, VKM Ac-2256, CGMCC 1.2323

Special Collections

Section

Nesterenkonia halobia 28-3 is an aerobe, Gram-positive, coccus-shaped bacterium that was isolated from unrefined solar salt.

coccus-shaped
Gram-positive
aerobe
16S sequence
Bacteria

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Actinomycetota*
	Class	*Actinomycetes*
	Order	*Micrococcales*
	Family	*Micrococcaceae*
	Genus	*Nesterenkonia*
	Species	**Nesterenkonia halobia**
	Full Scientific Name (LPSN)	Nesterenkonia halobia (Onishi and Kamekura 1972) Stackebrandt et al. 1995

Synonym

Micrococcus halobius

Information on morphological and physiological properties Morphology

[Ref.: #119799]	Gram stain	positive

[Ref.: #119799]	Cell shape	coccus-shaped

[Ref.: #119799]	Motility	no

[Ref.: #18756]	Colony color	Cream
[Ref.: #18756]	Incubation period	10-14 days
[Ref.: #18756]	Cultivation medium used	5006

[Ref.: #18756]	Colony color	Cream
[Ref.: #18756]	Incubation period	10-14 days
[Ref.: #18756]	Cultivation medium used	5428

[Ref.: #18756]	Colony color	Cream
[Ref.: #18756]	Incubation period	10-14 days
[Ref.: #18756]	Cultivation medium used	5428+6% NaCl

[Ref.: #18756]	Colony color	Cream
[Ref.: #18756]	Incubation period	10-14 days
[Ref.: #18756]	Cultivation medium used	5530

[Ref.: #18756]	Colony color	Beige
[Ref.: #18756]	Incubation period	10-14 days
[Ref.: #18756]	Cultivation medium used	ISP 2

[Ref.: #18756]	Multicellular complex forming ability	no
[Ref.: #18756]	Medium Name (multicellularity)	ISP 2

[Ref.: #18756]	Multicellular complex forming ability	no
[Ref.: #18756]	Medium Name (multicellularity)	5006

[Ref.: #18756]	Multicellular complex forming ability	no
[Ref.: #18756]	Medium Name (multicellularity)	5428+6% NaCl

[Ref.: #18756]	Multicellular complex forming ability	no
[Ref.: #18756]	Medium Name (multicellularity)	5428

[Ref.: #18756]	Multicellular complex forming ability	no
[Ref.: #18756]	Medium Name (multicellularity)	5530

Information on culture and growth conditions Culture and growth conditions

[Ref.: #8770]

Culture medium

BACTO MARINE BROTH (DIFCO 2216) (DSMZ Medium 514)

[Ref.: #8770]

Culture medium growth

[Ref.: #8770]

Culture medium link

Medium recipe at MediaDive

[Ref.: #8770]

Culture medium composition

expand / minimize

[Ref.: #18756]

Culture medium

ISP 2

[Ref.: #18756]

Culture medium growth

[Ref.: #18756]

Culture medium composition

expand / minimize

[Ref.: #18756]

Culture medium

5006

[Ref.: #18756]

Culture medium growth

[Ref.: #18756]

Culture medium composition

expand / minimize

[Ref.: #18756]

Culture medium

5428+6% NaCl

[Ref.: #18756]

Culture medium growth

[Ref.: #18756]

Culture medium

5428

[Ref.: #18756]

Culture medium growth

[Ref.: #18756]

Culture medium

5530

[Ref.: #18756]

Culture medium growth

[Ref.: #37624]

Culture medium

Marine agar (MA)

[Ref.: #37624]

Culture medium growth

[Ref.: #37624]

Culture medium composition

expand / minimize

[Ref.: #119799]

Culture medium

CIP Medium 13

[Ref.: #119799]

Culture medium growth

[Ref.: #119799]

Culture medium link

Medium recipe provided by DSMZ

		Temperature
[Ref.: #8770]	growth	28 °C
[Ref.: #18756]	optimum	28 °C
[Ref.: #37624]	growth	30 °C
[Ref.: #54531]	growth	30-37 °C
[Ref.: #67770]	growth	28 °C

Information on physiology and metabolism Physiology and metabolism

[Ref.: #54531]	Oxygen tolerance	aerobe
[Ref.: #119799]	Oxygen tolerance	obligate aerobe

[Ref.: #119799]

Ability of spore formation

[Ref.: #8770]	Name of produced compound	amylase, halophilic
[Ref.: #20216]	Name of produced compound	Amylase (halophilic)
[Ref.: #67770]	Name of produced compound	Halophilic amylase

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #54531]		NaCl		growth	>6	%

[Ref.: #8770]	Murein short key	A11.56
[Ref.: #8770]	Murein types	A4alpha L-Lys-Gly-L-Glu

[Ref.: #67770]

Observation

quinones: MK-8

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #68368]	arginine ChEBI	-	hydrolysis	* from API 20E
[Ref.: #68368]	citrate ChEBI	-	assimilation	* from API 20E
[Ref.: #68379]	D-glucose ChEBI	-	fermentation	* from API Coryne
[Ref.: #68379]	D-mannitol ChEBI	-	fermentation	* from API Coryne
[Ref.: #68379]	D-ribose ChEBI	-	fermentation	* from API Coryne
[Ref.: #68379]	D-xylose ChEBI	-	fermentation	* from API Coryne
[Ref.: #68379]	esculin ChEBI	+	hydrolysis	* from API Coryne
[Ref.: #68368]	gelatin ChEBI	-	hydrolysis	* from API 20E, API Coryne
[Ref.: #68379]	glycogen ChEBI	-	fermentation	* from API Coryne
[Ref.: #68379]	lactose ChEBI	-	fermentation	* from API Coryne
[Ref.: #68368]	lysine ChEBI	-	degradation	* from API 20E
[Ref.: #68379]	maltose ChEBI	-	fermentation	* from API Coryne
[Ref.: #119799]	nitrate ChEBI	-	reduction
[Ref.: #68379]	nitrate ChEBI	-	reduction	* from API Coryne
[Ref.: #119799]	nitrite ChEBI	-	reduction
[Ref.: #68368]	ornithine ChEBI	-	degradation	* from API 20E
[Ref.: #68379]	sucrose ChEBI	-	fermentation	* from API Coryne
[Ref.: #68368]	tryptophan ChEBI	-	energy source	* from API 20E
[Ref.: #68368]	urea ChEBI	-	hydrolysis	* from API 20E, API Coryne
	Only first 10 entries are displayed. Click here to see all.

	Metabolite	Production
[Ref.: #119799]	indole ChEBI	no
[Ref.: #68368]	acetoin ChEBI	yes	* from API 20E
[Ref.: #68368]	hydrogen sulfide ChEBI	no	* from API 20E
[Ref.: #68368]	indole ChEBI	no	* from API 20E

	Physiological tests	Metabolite	Voges-Proskauer-test	Indole test
[Ref.: #68368]		acetoin ChEBI	+		* from API 20E
[Ref.: #68368]		indole ChEBI		-	* from API 20E

	Enzyme	Enzyme activity	EC number
[Ref.: #68382]	acid phosphatase	-	3.1.3.2	* from API zym
[Ref.: #68379]	alkaline phosphatase	-	3.1.3.1	* from API Coryne
[Ref.: #68382]	alpha-fucosidase	-	3.2.1.51	* from API zym
[Ref.: #68382]	alpha-galactosidase	-	3.2.1.22	* from API zym
[Ref.: #68379]	alpha-glucosidase	+	3.2.1.20	* from API Coryne, API zym
[Ref.: #68382]	alpha-mannosidase	-	3.2.1.24	* from API zym
[Ref.: #68368]	arginine dihydrolase	-	3.5.3.6	* from API 20E
[Ref.: #68379]	beta-galactosidase	+	3.2.1.23	* from API Coryne
[Ref.: #68368]	beta-galactosidase	-	3.2.1.23	* from API 20E
[Ref.: #68379]	beta-glucosidase	+	3.2.1.21	* from API Coryne, API zym
[Ref.: #68379]	beta-glucuronidase	-	3.2.1.31	* from API Coryne, API zym
[Ref.: #119799]	catalase	+	1.11.1.6
[Ref.: #68379]	gelatinase	-		* from API Coryne, API 20E
[Ref.: #68382]	leucine arylamidase	+	3.4.11.1	* from API zym
[Ref.: #68368]	lysine decarboxylase	-	4.1.1.18	* from API 20E
[Ref.: #68379]	N-acetyl-beta-glucosaminidase	-	3.2.1.52	* from API Coryne, API zym
[Ref.: #68368]	ornithine decarboxylase	-	4.1.1.17	* from API 20E
[Ref.: #119799]	oxidase	+
[Ref.: #68379]	pyrazinamidase	-	3.5.1.B15	* from API Coryne
[Ref.: #68379]	pyrrolidonyl arylamidase	-	3.4.19.3	* from API Coryne
[Ref.: #68382]	trypsin	-	3.4.21.4	* from API zym
[Ref.: #68368]	tryptophan deaminase	-	4.1.99.1	* from API 20E
[Ref.: #119799]	urease	-	3.5.1.5
[Ref.: #68379]	urease	-	3.5.1.5	* from API Coryne, API 20E
[Ref.: #68382]	valine arylamidase	+		* from API zym
	Only first 10 entries are displayed. Click here to see all.

API® 20E:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	beta-GalactosidaseONPG	Arginine dihydrolaseADH (Arg)	Lysine decarboxylaseLDC (Lys)	Ornithine decarboxylaseODC	Citrate utilizationCIT	H2S productionH2S	Urease/urea hydrolysisURE	Tryptophan deaminaseTDA (Trp)	Indole productionIND	Acetoin production (Voges Proskauer test)VP	Gelatinase/gelatin hydrolysisGEL	Fermentation/oxidation of D-glucoseGLU	Fermentation/oxidation of D-mannitolMAN	Fermentation/oxidation of inositolINO	Fermentation/oxidation of sorbitolSor	Fermentation/oxidation of L-rhamnoseRHA	Fermentation/oxidation of sucroseSAC	Fermentation/oxidation of melibioseMEL	Fermentation/oxidation of amygdalinAMY	Fermentation/oxidation of L-arabinoseARA	Cytochrome oxidaseOX	Nitrite productionNO2	Reduction to N2N2	MotilityMOB	Growth on MacConkey mediumMAC	Oxidative catabolisation of D-glucoseOF-O	Fermentation of D-glucoseOF-F
[Ref.: #18756]	235	-	-	-	-	-	-	-	-	-	+	-	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.

API® Coryne:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Reduction of nitrateNIT	PyrazinamidasePYZ	Pyrrolidonyl arylamidasePYRA	Alkaline phosphatasePAL	beta-Glucuronidasebeta GUR	beta-Galactosidasebeta GAL	alpha-Glucosidasealpha GLU	N-Acetyl-beta-Glucosidasebeta NAG	Esculin hydrolysis/beta-GlucosidaseESC	Urease/urea hydrolysisURE	Gelatinase/gelatin hydrolysisGEL	Control fermentationControl	Fermentation of D-glucoseGLU	Fermentation of D-riboseRIB	Fermentation of D-xyloseXYL	Fermentation of D-mannitolMAN	Fermentation of maltoseMAL	Fermentation of lactoseLAC	Fermentation of sucroseSAC	Fermentation of glycogenGLYG	CatalaseCAT
[Ref.: #18756]	194	-	-	-	-	-	+	+	-	+	-	-	not determinedn.d.	-	-	-	-	-	-	-	-	not determinedn.d.

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #18756]	323	not determinedn.d.	-	-	-	-	+	+	-	-	-	-	-	-	-	-	+	+	-	-	-
[Ref.: #119799]	13495	-	+	+	+	+	+	+	+	-	+	-	+	-	+	-	+	+	-	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #8770]	Sample type/isolated from	unrefined solar salt

[Ref.: #54531]	Sample type/isolated from	Unrefined solar salt

[Ref.: #67770]	Sample type/isolated from	Unrefined solar salt
[Ref.: #67770]	Geographic location (country and/or sea, region)	Noda, Chiba Pref.
[Ref.: #67770]	Country	Japan
[Ref.: #67770]	Country ISO 3 Code	JPN
[Ref.: #67770]	Continent	Asia

[Ref.: #119799]	Sample type/isolated from	Unrefined solar salt
* marker position based on {}

Isolation sources categories

#Condition

#Saline

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence X80747 (>99% sequence identity) for Nesterenkonia from Microbeatlas

Aquatic Samples	234
Soil Samples	126
Animal Samples	1996
Plant Samples	70
Total Samples	2426

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #18756]	Biosafety level	1	Risk group (German classification)
[Ref.: #8770]	Biosafety level	1	Risk group (German classification) According to TRBA 466
[Ref.: #119799]	Biosafety level	1	Risk group (French classification)

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #20218]	M.halobius 16S rDNA	X80747	1451	GenBank ENA	37922 tax ID	*

[Ref.: #8770]	GC-content	71.5 mol%
[Ref.: #67770]	GC-content	70 mol%	fluorimetric
[Ref.: #67770]	GC-content	71.5 mol%	thermal denaturation, midpoint method (Tm)

Availability in culture collections External links

[Ref.: #8770]

Culture collection no.

DSM 20541, ATCC 21727, CCM 2591, CCUG 38889, JCM 11483, BCRC 11656, CECT 5018, CGMCC 1.2323, CIP 81.68, IAM 13001, IFO 15353, IMET 11383, IMSNU 21325, KCTC 3518, LMG 14214, LMG 21452, MTCC 701, NBRC 15353, NCIMB 2204, VKM Ac-2256, VKM B-1317, VKM B-1421

[Ref.: #77205]

SI-ID 9303

Literature:

Topic	Title	Authors	Journal	DOI	Year
Metabolism	Cloning and characterization of the gene cluster for biosynthesis of ectoine from Nesterenkonia halobia DSM 20541.	Zhang B, Bao X, Wang L, Yang SS	J Microbiol	10.1007/s12275-008-0001-x	2008	*
Phylogeny	Nesterenkonia halophila sp. nov., a moderately halophilic, alkalitolerant actinobacterium isolated from a saline soil.	Li WJ, Zhang YQ, Schumann P, Liu HY, Yu LY, Zhang YQ, Stackebrandt E, Xu LH, Jiang CL	Int J Syst Evol Microbiol	10.1099/ijs.0.64226-0	2008	*
Phylogeny	[Study on isolation and systematic taxonomy of strains of genus Nesterenkonia].	Chen HH, Li WJ, Zhang YQ, Wang D, Tang SK	Wei Sheng Wu Xue Bao		2004	*
Phylogeny	Polyphasic taxonomy of Nesterenkonia halobia.	Mota RR, Marquez MC, Arahal DR, Mellado E, Ventosa A	Int J Syst Bacteriol	10.1099/00207713-47-4-1231	1997	*
Enzymology	Purification and Some Properties of an Extracellular Amylase from a Moderate Halophile, Micrococcus halobius.	Onishi H, Sonoda K	Appl Environ Microbiol	10.1128/aem.38.4.616-620.1979	1979	*

References

#8770

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 20541

#18756

Wink, J.: Compendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig .

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#20216

Leibniz-Institut für Naturstoff-Forschung und Infektionsbiologie e. V. Hans-Knöll-Institut (HKI) Curators of the HKI: Collection Description Leibniz-Institut für Naturstoff-Forschung und Infektionsbiologie e. V. Hans-Knöll-Institut (HKI) .

#20218

Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )

#37624 ; Curators of the CIP;
#54531 Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 38889
#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#68368

Automatically annotated from API 20E .

#68379

Automatically annotated from API Coryne .

#68382

Automatically annotated from API zym .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#77205

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID9303.1 )

#119799 Collection of Institut Pasteur ; Curators of the CIP; CIP 81.68
* These data were automatically processed and therefore are not curated

Change proposal

You found an error in BacDive? Please tell us about it!

Note that changes will be reviewed and judged. If your changes are legitimate, changes will occur within the next BacDive update. Only proposed changes supported by the according reference will be reviewed. The BacDive team reserves the right to reject proposed changes.

Search for species Nesterenkonia halobia in external resources:
SILVA
BRENDA
PANGAEA
GBIF