Name: Nesterenkonia lacusekhoensis EL-30T
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 7763

Type strain:

Species: Nesterenkonia lacusekhoensis

Strain Designation: EL-30T

Culture col. no.: CCUG 47141, JCM 11953, DSM 12544, CIP 107030, IFAM EL-30

Strain history: CIP <- 2001, P. Hirsch, Kiel Univ., Kiel, Germany: strain EL-30T

NCBI tax ID(s): 150832 (species)

SI-ID 100807

CIP 107030, DSM 12544, JCM 11953, CCUG 47141

Other strains from Nesterenkonia lacusekhoensis

N. lacusekhoensis CCUG 56670

Section

Nesterenkonia lacusekhoensis EL-30T is an aerobe, psychrophilic bacterium that was isolated from hypersaline lake.

aerobe
psychrophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Actinomycetota*
	Class	*Actinomycetes*
	Order	*Micrococcales*
	Family	*Micrococcaceae*
	Genus	*Nesterenkonia*
	Species	**Nesterenkonia lacusekhoensis**
	Full Scientific Name (LPSN)	Nesterenkonia lacusekhoensis Collins et al. 2002

Information on morphological and physiological properties Morphology

[Ref.: #120542]	Gram stain	positive
[Ref.: #69480]	Gram stain	positive Confidence in %100

[Ref.: #120542]	Cell shape	coccus-shaped

[Ref.: #120542]	Motility	no
[Ref.: #69480]	Motility	no Confidence in %92.638

[Ref.: #18369]	Colony color	Lemon yellow (1012)
[Ref.: #18369]	Incubation period	10-14 days
[Ref.: #18369]	Cultivation medium used	ISP 2

[Ref.: #18369]	Colony color	Beige (1001)
[Ref.: #18369]	Incubation period	10-14 days
[Ref.: #18369]	Cultivation medium used	ISP 3

[Ref.: #57534]	Incubation period	1 day

[Ref.: #18369]	Multicellular complex forming ability	no
[Ref.: #18369]	Medium Name (multicellularity)	ISP 2

[Ref.: #18369]	Multicellular complex forming ability	no
[Ref.: #18369]	Medium Name (multicellularity)	ISP 3

Information on culture and growth conditions Culture and growth conditions

[Ref.: #4760]

Culture medium

BACTO MARINE BROTH (DIFCO 2216) (DSMZ Medium 514)

[Ref.: #4760]

Culture medium growth

[Ref.: #4760]

Culture medium link

Medium recipe at MediaDive

[Ref.: #4760]

Culture medium composition

expand / minimize

[Ref.: #18369]

Culture medium

ISP 2

[Ref.: #18369]

Culture medium growth

[Ref.: #18369]

Culture medium composition

expand / minimize

[Ref.: #18369]

Culture medium

ISP 3

[Ref.: #18369]

Culture medium growth

[Ref.: #18369]

Culture medium composition

expand / minimize

[Ref.: #40660]

Culture medium

MEDIUM 72- for trypto casein soja agar

[Ref.: #40660]

Culture medium growth

[Ref.: #40660]

Culture medium composition

expand / minimize

[Ref.: #120542]

Culture medium

CIP Medium 72

[Ref.: #120542]

Culture medium growth

[Ref.: #120542]

Culture medium link

Medium recipe provided by DSMZ

	Kind of temperature		Temperature
[Ref.: #4760]		growth	20 °C
[Ref.: #18369]		optimum	22 °C
[Ref.: #40660]		growth	30 °C
[Ref.: #57534]		growth	30 °C
[Ref.: #67770]		growth	28 °C
[Ref.: #120542]		growth	22-41 °C
[Ref.: #120542]	no	growth	10 °C
[Ref.: #120542]	no	growth	45 °C

[Ref.: #4760]	Temperature range	psychrophilic
[Ref.: #18369]	Temperature range	psychrophilic
[Ref.: #40660]	Temperature range	mesophilic
[Ref.: #57534]	Temperature range	mesophilic
[Ref.: #67770]	Temperature range	mesophilic
[Ref.: #120542]	Temperature range	psychrophilic
[Ref.: #120542]	Temperature range	thermophilic

Information on physiology and metabolism Physiology and metabolism

[Ref.: #57534]	Oxygen tolerance	aerobe
[Ref.: #120542]	Oxygen tolerance	obligate aerobe

[Ref.: #69481]	Ability of spore formation	no Confidence in %100
[Ref.: #69480]	Ability of spore formation	no Confidence in %99.998

[Ref.: #4760]	Murein short key	A11.54
[Ref.: #4760]	Murein types	A4alpha L-Lys-L-Glu

[Ref.: #67770]

Observation

quinones: MK-7, MK-8

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #68379]	D-glucose ChEBI	+	fermentation	* from API Coryne
[Ref.: #68379]	D-mannitol ChEBI	-	fermentation	* from API Coryne
[Ref.: #68379]	D-ribose ChEBI	-	fermentation	* from API Coryne
[Ref.: #68379]	D-xylose ChEBI	-	fermentation	* from API Coryne
[Ref.: #68379]	esculin ChEBI	-	hydrolysis	* from API Coryne
[Ref.: #68379]	gelatin ChEBI	-	hydrolysis	* from API Coryne
[Ref.: #68379]	glycogen ChEBI	-	fermentation	* from API Coryne
[Ref.: #120542]	hippurate ChEBI	+	hydrolysis
[Ref.: #68379]	lactose ChEBI	-	fermentation	* from API Coryne
[Ref.: #68379]	maltose ChEBI	-	fermentation	* from API Coryne
[Ref.: #120542]	nitrate ChEBI	-	reduction
[Ref.: #68379]	nitrate ChEBI	-	reduction	* from API Coryne
[Ref.: #120542]	nitrite ChEBI	-	reduction
[Ref.: #68379]	sucrose ChEBI	+	fermentation	* from API Coryne
[Ref.: #68379]	urea ChEBI	-	hydrolysis	* from API Coryne
	Only first 10 entries are displayed. Click here to see all.

	Metabolite production	Metabolite	Production
[Ref.: #120542]		indole ChEBI	no

	Physiological tests	Metabolite	Voges-Proskauer-test
[Ref.: #120542]		acetoin ChEBI	-

	Enzyme	Enzyme activity	EC number
[Ref.: #68382]	acid phosphatase	-	3.1.3.2	* from API zym
[Ref.: #120542]	alcohol dehydrogenase	-	1.1.1.1
[Ref.: #68382]	alpha-chymotrypsin	-	3.4.21.1	* from API zym
[Ref.: #68382]	alpha-fucosidase	-	3.2.1.51	* from API zym
[Ref.: #68382]	alpha-galactosidase	-	3.2.1.22	* from API zym
[Ref.: #68379]	alpha-glucosidase	+	3.2.1.20	* from API Coryne, API zym
[Ref.: #68382]	alpha-mannosidase	-	3.2.1.24	* from API zym
[Ref.: #120542]	amylase	-
[Ref.: #120542]	beta-galactosidase	-	3.2.1.23
[Ref.: #68379]	beta-galactosidase	-	3.2.1.23	* from API Coryne, API zym
[Ref.: #68379]	beta-glucosidase	-	3.2.1.21	* from API Coryne
[Ref.: #68379]	beta-glucuronidase	-	3.2.1.31	* from API Coryne
[Ref.: #120542]	caseinase	-	3.4.21.50
[Ref.: #120542]	catalase	+	1.11.1.6
[Ref.: #120542]	coagulase	-
[Ref.: #68382]	cystine arylamidase	-	3.4.11.3	* from API zym
[Ref.: #120542]	DNase	-
[Ref.: #68382]	esterase (C 4)	+		* from API zym
[Ref.: #68382]	esterase lipase (C 8)	+		* from API zym
[Ref.: #120542]	gamma-glutamyltransferase	-	2.3.2.2
[Ref.: #120542]	gelatinase	-
[Ref.: #68379]	gelatinase	-		* from API Coryne
[Ref.: #68382]	leucine arylamidase	+	3.4.11.1	* from API zym
[Ref.: #68382]	lipase (C 14)	-		* from API zym
[Ref.: #120542]	lysine decarboxylase	-	4.1.1.18
[Ref.: #68379]	N-acetyl-beta-glucosaminidase	-	3.2.1.52	* from API Coryne, API zym
[Ref.: #120542]	ornithine decarboxylase	-	4.1.1.17
[Ref.: #120542]	oxidase	-
[Ref.: #120542]	phenylalanine ammonia-lyase	-	4.3.1.24
[Ref.: #68379]	pyrazinamidase	-	3.5.1.B15	* from API Coryne
[Ref.: #68379]	pyrrolidonyl arylamidase	-	3.4.19.3	* from API Coryne
[Ref.: #120542]	tween esterase	-
[Ref.: #120542]	urease	-	3.5.1.5
[Ref.: #68379]	urease	-	3.5.1.5	* from API Coryne
	Only first 10 entries are displayed. Click here to see all.

API® Coryne:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Reduction of nitrateNIT	PyrazinamidasePYZ	Pyrrolidonyl arylamidasePYRA	Alkaline phosphatasePAL	beta-Glucuronidasebeta GUR	beta-Galactosidasebeta GAL	alpha-Glucosidasealpha GLU	N-Acetyl-beta-Glucosidasebeta NAG	Esculin hydrolysis/beta-GlucosidaseESC	Urease/urea hydrolysisURE	Gelatinase/gelatin hydrolysisGEL	Control fermentationControl	Fermentation of D-glucoseGLU	Fermentation of D-riboseRIB	Fermentation of D-xyloseXYL	Fermentation of D-mannitolMAN	Fermentation of maltoseMAL	Fermentation of lactoseLAC	Fermentation of sucroseSAC	Fermentation of glycogenGLYG	CatalaseCAT
[Ref.: #18369]	56	-	-	-	+/-	-	-	+	-	-	-	-	not determinedn.d.	+	-	-	-	-	-	+	-	not determinedn.d.

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #18369]	79	not determinedn.d.	+	+	+	-	+	+	-	+	-	-	+	-	-	+	+	+	-	-	-
[Ref.: #120542]	10013	-	-	+	+	-	+	-	-	-	-	-	-	-	-	-	+	-	-	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #4760]	Sample type/isolated from	hypersaline lake
[Ref.: #4760]	Geographic location (country and/or sea, region)	East Antarctica, Vestfold Hills, Ekho Lake
[Ref.: #4760]	Continent	Australia and Oceania

[Ref.: #57534]	Sample type/isolated from	Water
[Ref.: #57534]	Sampling date	1990
[Ref.: #57534]	Geographic location (country and/or sea, region)	East Antarctica,Ekho Lake
[Ref.: #57534]	Country	Antarctica
[Ref.: #57534]	Country ISO 3 Code	ATA
[Ref.: #57534]	Continent	Australia and Oceania

[Ref.: #67770]	Sample type/isolated from	23 m water sample of the hypersaline Ekho Lake
[Ref.: #67770]	Geographic location (country and/or sea, region)	Vestfold Hills
[Ref.: #67770]	Country	Antarctica
[Ref.: #67770]	Country ISO 3 Code	ATA
[Ref.: #67770]	Continent	Antarctica

[Ref.: #120542]	Sample type/isolated from	Environment, Water
[Ref.: #120542]	Geographic location (country and/or sea, region)	Ekho lake
[Ref.: #120542]	Country	Antarctica
[Ref.: #120542]	Country ISO 3 Code	ATA
[Ref.: #120542]	Continent	Antarctica
[Ref.: #120542]	Isolation date	1990
* marker position based on {}

Isolation sources categories

#Environmental	#Aquatic	#Lake (large)
#Condition	#Saline	-

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence AJ290397 (>99% sequence identity) for Nesterenkonia from Microbeatlas

Aquatic Samples	399
Soil Samples	373
Animal Samples	3735
Plant Samples	100
Total Samples	4607

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #18369]	Biosafety level	1
[Ref.: #4760]	Biosafety level	1	Risk group (German classification) According to TRBA 466
[Ref.: #120542]	Biosafety level	1	Risk group (French classification)

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #4760]	Nesterenkonia sp. EL-30 16S rRNA gene, strain EL-30	AJ290397	1468	ENA	150832 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Nesterenkonia lacusekhoensis DSM 12544	GCA_017876395	contig	GenBank	150832 tax ID	*
[Ref.: #66792]	Nesterenkonia lacusekhoensis strain DSM 12544	150832.3	wgs	PATRIC	150832 tax ID	*
[Ref.: #66792]	Nesterenkonia lacusekhoensis DSM 12544	2918341021	draft	JGI IMG/M	150832 tax ID	*

[Ref.: #67770]

GC-content

66.1 mol%

high performance liquid chromatography (HPLC)

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Nesterenkonia lacusekhoensis strain DSM 12544 PATRIC: 150832.3
[Ref.: #69481]	spore-forming	no	100	no

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Nesterenkonia lacusekhoensis strain DSM 12544 PATRIC: 150832.3
[Ref.: #69480]	motile	no	93.402	no
[Ref.: #69480]	gram-positive	yes	92.938	no
[Ref.: #69480]	anaerobic	no	99.493	no
[Ref.: #69480]	aerobic	yes	94.576	no
[Ref.: #69480]	halophile	yes	88.615	no
[Ref.: #69480]	spore-forming	no	94.401	no
[Ref.: #69480]	thermophile	no	96.573	no
[Ref.: #69480]	glucose-util	yes	84.873	no
[Ref.: #69480]	flagellated	no	96.886	no
[Ref.: #69480]	glucose-ferment	no	82.392	yes

Availability in culture collections External links

[Ref.: #4760]

Culture collection no.

CCUG 47141, JCM 11953, DSM 12544, CIP 107030, IFAM EL-30

[Ref.: #77206]

SI-ID 100807

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	[Study on isolation and systematic taxonomy of strains of genus Nesterenkonia].	Chen HH, Li WJ, Zhang YQ, Wang D, Tang SK	Wei Sheng Wu Xue Bao		2004	*
Phylogeny	Nesterenkonia lacusekhoensis sp. nov., isolated from hypersaline Ekho Lake, East Antarctica, and emended description of the genus Nesterenkonia.	Collins MD, Lawson PA, Labrenz M, Tindall BJ, Weiss N, Hirsch P	Int J Syst Evol Microbiol	10.1099/00207713-52-4-1145	2002	*

References

#4760

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 12544

#18369

Wink, J.: Compendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig .

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#40660 ; Curators of the CIP;
#57534 Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 47141

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#68379

Automatically annotated from API Coryne .

#68382

Automatically annotated from API zym .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#77206

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID100807.1 )

#120542 Collection of Institut Pasteur ; Curators of the CIP; CIP 107030
* These data were automatically processed and therefore are not curated

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Nesterenkonia lacusekhoensis strain DSM 12544

Nesterenkonia lacusekhoensis strain DSM 12544

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