Nesterenkonia lacusekhoensis (CCUG 47141, JCM 11953, DSM 12544)

Name: Nesterenkonia lacusekhoensis (CCUG 47141, JCM 11953, DSM 12544)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 7763

Type strain

Strain Designation EL-30T

Culture col. no. CCUG 47141 JCM 11953 DSM 12544 CIP 107030 IFAM EL-30 1 more

NCBI tax ID(s) 150832

Special Collections DSMZ CCUG JCM CIP Wink compendium

History <- M. Labrenz and P. Hirsch, Inst. Allg. Mikrobiol. Univ. Kiel; IFAM EL-30 CIP 107030 <-- P. Hirsch EL-30. CIP <- 2001, P. Hirsch, Kiel Univ., Kiel, Germany: strain EL-30T

Links

Nesterenkonia lacusekhoensis EL-30T is an aerobe, Gram-positive, coccus-shaped bacterium that was isolated from hypersaline lake.

Gram-positive coccus-shaped aerobe genome sequence 16S sequence Bacteria

Name and taxonomic classification

SI-ID 100807

CIP 107030,DSM 12544,JCM 11953,CCUG 47141

@ref 20215

Last LPSN update 2026-02-09 11:19:33

Domain Bacteria

Phylum Actinomycetota

Class Actinomycetes

Order Micrococcales

Family Micrococcaceae

Genus Nesterenkonia

Species Nesterenkonia lacusekhoensis

Full scientific name Nesterenkonia lacusekhoensis Collins et al. 2002

BacDive ID	Other strains from **Nesterenkonia lacusekhoensis** (1)	Type strain
155163	N. lacusekhoensis CCUG 56670

Morphology

Cell morphology

@ref	Gram stain	Cell shape	Confidence
120542	positive	coccus-shaped
125438			91
125439	positive		94.5

Colony morphology

@ref	Colony color	Incubation period	Medium used
18369	Lemon yellow (1012)	10-14 days	ISP 2
18369	Beige (1001)	10-14 days	ISP 3
57534		1 day
120542

Multicellular morphology

@ref	Forms multicellular complex	Medium name
18369		ISP 2
18369		ISP 3

Culture and growth conditions

Culture medium

@ref	Name	Medium link	Composition
4760	BACTO MARINE BROTH (DIFCO 2216) (DSMZ Medium 514)	Medium recipe at MediaDive	Name: BACTO MARINE BROTH (DIFCO 2216) (DSMZ Medium 514) Composition: NaCl 19.45 g/l MgCl2 5.9 g/l Bacto peptone 5.0 g/l Na2SO4 3.24 g/l CaCl2 1.8 g/l Yeast extract 1.0 g/l KCl 0.55 g/l NaHCO3 0.16 g/l Fe(III) citrate 0.1 g/l KBr 0.08 g/l SrCl2 0.034 g/l H3BO3 0.022 g/l Na2HPO4 0.008 g/l Na-silicate 0.004 g/l NaF 0.0024 g/l (NH4)NO3 0.0016 g/l Distilled water
18369	ISP 2		Name: ISP 2 / Yeast Malt Agar (5265); 5265 Composition Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l Agar 15.0 g/l Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.0 Usage: Maintenance and Taxonomy Organisms: All Actinomycetes
18369	ISP 3		Name: ISP 3; 5315 Composition Dog oat flakes 20.0 g/l Trace element solution (5314) 2.5 ml/l Agar 18.0 g/l Preparation: Oat flakes are cooked for 20 minutes, trace element solution and agar are added (in the case of non rolled oat flakes the suspension has to bee filtrated). Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.8 Usage: Maintenance and taxonomy (e.g. SEM As liquid medium for metabolite production) Organisms: All Actinomycetes Trace element solution 5314 Name: Trace element solution 5314; 5314 Composition CaCl2 x H2O 3.0 g/l Fe-III-citrate 1.0 g/l MnSO4 0.2 g/l ZnCl2 0.1 g/l CuSO4 x 5 H2O 0.025 g/l Sodium tetra borate 0.2 g/l CoCl2 x 6 H2O 0.004 g/l Sodium molybdate 0.01 g/l Preparation: Use double destillated water. Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Trace element solution for different media Organisms:
40660	MEDIUM 72- for trypto casein soja agar		Distilled water make up to (1000.000 ml);Trypto casein soy agar (40.000 g)
120542	CIP Medium 72	Medium recipe at CIP

Culture temp

@ref	Growth	Type	Temperature (°C)
4760	positive	growth	20
18369	positive	optimum	22
40660	positive	growth	30
57534	positive	growth	30
67770	positive	growth	28
120542	positive	growth	22-41
120542	negative	growth	10
120542	negative	growth	45

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance
57534	aerobe
120542	obligate aerobe

Murein

@ref	Murein short key	Type
4760	A11.54	A4alpha L-Lys-L-Glu

Observation

67770

Observationquinones: MK-7, MK-8

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
68379	17634 ChEBI	D-glucose	+	fermentation	from API Coryne
68379	16899 ChEBI	D-mannitol	-	fermentation	from API Coryne
68379	16988 ChEBI	D-ribose	-	fermentation	from API Coryne
68379	65327 ChEBI	D-xylose	-	fermentation	from API Coryne
68379	4853 ChEBI	esculin	-	hydrolysis	from API Coryne
68379	5291 ChEBI	gelatin	-	hydrolysis	from API Coryne
68379	28087 ChEBI	glycogen	-	fermentation	from API Coryne
120542	606565 ChEBI	hippurate	+	hydrolysis
68379	17716 ChEBI	lactose	-	fermentation	from API Coryne
68379	17306 ChEBI	maltose	-	fermentation	from API Coryne
68379	17632 ChEBI	nitrate	-	reduction	from API Coryne
120542	17632 ChEBI	nitrate	-	reduction
120542	16301 ChEBI	nitrite	-	reduction
68379	17992 ChEBI	sucrose	+	fermentation	from API Coryne
68379	16199 ChEBI	urea	-	hydrolysis	from API Coryne

Metabolite production

@ref	Chebi-ID	Metabolite	Production
120542	35581 ChEBI	indole

Metabolite tests

@ref	Chebi-ID	Metabolite	Voges-proskauer-test
120542	15688 ChEBI	acetoin	-

Enzymes

@ref	Value	Activity	Ec
68382	acid phosphatase	-	3.1.3.2	from API zym
120542	alcohol dehydrogenase	-	1.1.1.1
68382	alpha-chymotrypsin	-	3.4.21.1	from API zym
68382	alpha-fucosidase	-	3.2.1.51	from API zym
68382	alpha-galactosidase	-	3.2.1.22	from API zym
68382	alpha-glucosidase	+	3.2.1.20	from API zym
68379	alpha-glucosidase	+	3.2.1.20	from API Coryne
68382	alpha-mannosidase	-	3.2.1.24	from API zym
120542	amylase	-
68382	beta-galactosidase	-	3.2.1.23	from API zym
120542	beta-galactosidase	-	3.2.1.23
68379	beta-galactosidase	-	3.2.1.23	from API Coryne
68379	beta-glucosidase	-	3.2.1.21	from API Coryne
68379	beta-glucuronidase	-	3.2.1.31	from API Coryne
120542	caseinase	-	3.4.21.50
120542	catalase	+	1.11.1.6
120542	coagulase	-
68382	cystine arylamidase	-	3.4.11.3	from API zym
120542	DNase	-
68382	esterase (C 4)	+		from API zym
68382	esterase lipase (C 8)	+		from API zym
120542	gamma-glutamyltransferase	-	2.3.2.2
120542	gelatinase	-
68379	gelatinase	-		from API Coryne
68382	leucine arylamidase	+	3.4.11.1	from API zym
68382	lipase (C 14)	-		from API zym
120542	lysine decarboxylase	-	4.1.1.18
68382	N-acetyl-beta-glucosaminidase	-	3.2.1.52	from API zym
68379	N-acetyl-beta-glucosaminidase	-	3.2.1.52	from API Coryne
120542	ornithine decarboxylase	-	4.1.1.17
120542	oxidase	-
120542	phenylalanine ammonia-lyase	-	4.3.1.24
68379	pyrazinamidase	-	3.5.1.B15	from API Coryne
68379	pyrrolidonyl arylamidase	-	3.4.19.3	from API Coryne
120542	tween esterase	-
120542	urease	-	3.5.1.5
68379	urease	-	3.5.1.5	from API Coryne

API coryne

@ref	Reduction of nitrateNIT	PYZ	PYRA	PAL	beta GUR	beta GAL	alpha GLU	beta NAG	ESC	URE	GEL	Control fermentationControl	GLU	RIB	XYL	MAN	MAL	LAC	SAC	GLYG	CAT
18369	-	-	-	+/-	-	-	+	-	-	-	-	not determinedn.d.	+	-	-	-	-	-	+	-	not determinedn.d.

API zym

@ref	Control	Alkaline phosphatase	Esterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	Lipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	Trypsin	alpha- Chymotrypsin	Acid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	alpha- Galactosidase	beta- Galactosidase	beta- Glucuronidase	alpha- Glucosidase	beta- Glucosidase	N-acetyl-beta- glucosaminidase	alpha- Mannosidase	alpha- Fucosidase
18369	not determinedn.d.	+	+	+	-	+	+	-	+	-	-	+	-	-	+	+	+	-	-	-
120542	-	-	+	+	-	+	-	-	-	-	-	-	-	-	-	+	-	-	-	-

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Environmental	#Aquatic	#Lake (large)
#Condition	#Saline	-

Isolation

@ref	Sample type	Sampling date	Geographic location	Country	Country ISO 3 Code	Continent	Isolation date
4760	hypersaline lake		East Antarctica, Vestfold Hills, Ekho Lake			Australia and Oceania
57534	Water	1990	East Antarctica,Ekho Lake	Antarctica	ATA	Australia and Oceania
67770	23 m water sample of the hypersaline Ekho Lake		Vestfold Hills	Antarctica	ATA	Antarctica
120542	Environment, Water		Ekho lake	Antarctica	ATA	Antarctica	1990

Taxonmaps

69479

Global distribution of 16S sequence AJ290397 (>99% sequence identity) for Nesterenkonia from Microbeatlas

Aquatic Samples	399
Soil Samples	373
Animal Samples	3735
Plant Samples	100
Total Samples	4607

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
4760	1	Risk group (German classification) According to TRBA 466
18369	1
120542	1	Risk group (French classification) According to TRBA 466

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
66792	ASM1787639v1 assembly for Nesterenkonia lacusekhoensis DSM 12544	contig	GCA_017876395	150832.3	2918341021	150832	72.95
124043	ASM4266018v1 assembly for Nesterenkonia lacusekhoensis CCUG 47141	scaffold	GCA_042660185			150832	50.88
124043	ASM3952706v1 assembly for Nesterenkonia lacusekhoensis JCM 11953	scaffold	GCA_039527065			150832	26.44

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
4760	Nesterenkonia sp. EL-30 16S rRNA gene, strain EL-30	AJ290397	1468		150832

GC content

@ref	GC-content (mol%)	Method
67770	66.1	high performance liquid chromatography (HPLC)

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: Nesterenkonia lacusekhoensis DSM 12544
NCBI: GCA_017876395

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	spore_formation	BacteriaNetⓘ	no	84.80	no
125439	motility	BacteriaNetⓘ	no	62.90	no
125439	gram_stain	BacteriaNetⓘ	positive	94.50	no
125439	oxygen_tolerance	BacteriaNetⓘ	facultative anaerobe	69.20	no

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Nesterenkonia lacusekhoensis strain DSM 12544
PATRIC: 150832.3

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	yes	87.94	no
125438	anaerobic	anaerobicⓘ	no	97.57	yes
125438	spore-forming	spore-formingⓘ	no	78.23	no
125438	aerobic	aerobicⓘ	yes	84.57	yes
125438	thermophilic	thermophileⓘ	no	92.36	yes
125438	flagellated	motile2+ⓘ	no	91.00	no

Literature

Topic	Title	Authors	Journal	DOI	Year
Genetics	Comparative Genomic Analyses of the Genus Nesterenkonia Unravels the Genomic Adaptation to Polar Extreme Environments.	Dai D, Lu H, Xing P, Wu Q.	Microorganisms	10.3390/microorganisms10020233	2022
Phylogeny	[Study on isolation and systematic taxonomy of strains of genus Nesterenkonia].	Chen HH, Li WJ, Zhang YQ, Wang D, Tang SK	Wei Sheng Wu Xue Bao		2004
Phylogeny	Nesterenkonia lacusekhoensis sp. nov., isolated from hypersaline Ekho Lake, East Antarctica, and emended description of the genus Nesterenkonia.	Collins MD, Lawson PA, Labrenz M, Tindall BJ, Weiss N, Hirsch P	Int J Syst Evol Microbiol	10.1099/00207713-52-4-1145	2002

References

#4760	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 12544
#18369	Wink, J.: Compendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig .
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#40660	; Curators of the CIP;
#57534	Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 47141
#66792	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .
#67770	Japan Collection of Microorganism (JCM) ; Curators of the JCM;
#68379	Automatically annotated from API Coryne .
#68382	Automatically annotated from API zym .
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#120542	Collection of Institut Pasteur ; Curators of the CIP; CIP 107030
#124043	Isabel Schober, Julia Koblitz: Data extracted from sequence databases, automatically matched based on designation and taxonomy .
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Nesterenkonia lacusekhoensis (CCUG 47141, JCM 11953, DSM 12544)

Name and taxonomic classification

Morphology

Cell morphology

Colony morphology

Multicellular morphology

Culture and growth conditions

Culture medium

Culture temp

Physiology and metabolism

Oxygen tolerance

Murein

Observation

Metabolite utilization

Metabolite production

Metabolite tests

Enzymes

API coryne

API zym

Isolation, sampling and environmental information

Isolation source categories

Isolation

Taxonmaps

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_017876395

16S sequences

GC content

Genome-based predictions

Predictions

Literature

Literature

References

BacDive

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