Name: Arthrobacter castelli R-5102
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 7603

Type strain:

Species: Arthrobacter castelli

Strain Designation: R-5102

Culture col. no.: DSM 16402, LMG 22283, JCM 21794, IAM 15349, CIP 108896, LMG 19502

Strain history: CIP <- 2005, DSMZ

NCBI tax ID(s): 1121019 (strain), 271431 (species)

SI-ID 132113

DSM 16402, LMG 22283, JCM 21794, IAM 15349

Section

Arthrobacter castelli R-5102 is an aerobe, mesophilic, Gram-positive bacterium that was isolated from Mural painting, biofilm.

Gram-positive
rod-shaped
aerobe
mesophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Actinomycetota*
	Class	*Actinomycetes*
	Order	*Micrococcales*
	Family	*Micrococcaceae*
	Genus	*Arthrobacter*
	Species	**Arthrobacter castelli**
	Full Scientific Name (LPSN)	Arthrobacter castelli Heyrman et al. 2005

Information on morphological and physiological properties Morphology

[Ref.: #31342]	Gram stain	positive
[Ref.: #117208]	Gram stain	positive
[Ref.: #69480]	Gram stain	positive Confidence in %100

[Ref.: #31342]	Cell width	0.9 µm

[Ref.: #31342]	Cell shape	rod-shaped
[Ref.: #117208]	Cell shape	coccus-shaped

[Ref.: #31342]	Motility	no
[Ref.: #117208]	Motility	no

[Ref.: #18296]	Colony color	Honey yellow (1005)
[Ref.: #18296]	Incubation period	10-14 days
[Ref.: #18296]	Cultivation medium used	ISP 2

[Ref.: #18296]	Colony color	Honey yellow (1005)
[Ref.: #18296]	Incubation period	10-14 days
[Ref.: #18296]	Cultivation medium used	ISP 3

[Ref.: #18296]	Colony color	Cream (9001)
[Ref.: #18296]	Incubation period	10-14 days
[Ref.: #18296]	Cultivation medium used	ISP 4

[Ref.: #18296]	Colony color	Honey yellow (1005)
[Ref.: #18296]	Incubation period	10-14 days
[Ref.: #18296]	Cultivation medium used	ISP 5

[Ref.: #18296]	Colony color	Lemon yellow (1012)
[Ref.: #18296]	Incubation period	10-14 days
[Ref.: #18296]	Cultivation medium used	ISP 6

[Ref.: #18296]	Colony color	Lemon yellow (1012)
[Ref.: #18296]	Incubation period	10-14 days
[Ref.: #18296]	Cultivation medium used	ISP 7

[Ref.: #6431]	Incubation period	8-14 days

[Ref.: #31342]

Pigment production

[Ref.: #18296]	Multicellular complex forming ability	no
[Ref.: #18296]	Medium Name (multicellularity)	ISP 2

[Ref.: #18296]	Multicellular complex forming ability	no
[Ref.: #18296]	Medium Name (multicellularity)	ISP 3

[Ref.: #18296]	Multicellular complex forming ability	no
[Ref.: #18296]	Medium Name (multicellularity)	ISP 4

[Ref.: #18296]	Multicellular complex forming ability	no
[Ref.: #18296]	Medium Name (multicellularity)	ISP 5

[Ref.: #18296]	Multicellular complex forming ability	no
[Ref.: #18296]	Medium Name (multicellularity)	ISP 6

[Ref.: #18296]	Multicellular complex forming ability	no
[Ref.: #18296]	Medium Name (multicellularity)	ISP 7

Information on culture and growth conditions Culture and growth conditions

[Ref.: #6431]

Culture medium

BACTO MARINE BROTH (DIFCO 2216) (DSMZ Medium 514)

[Ref.: #6431]

Culture medium growth

[Ref.: #6431]

Culture medium link

Medium recipe at MediaDive

[Ref.: #6431]

Culture medium composition

expand / minimize

[Ref.: #18296]

Culture medium

ISP 2

[Ref.: #18296]

Culture medium growth

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Culture medium composition

expand / minimize

[Ref.: #18296]

Culture medium

ISP 3

[Ref.: #18296]

Culture medium growth

[Ref.: #18296]

Culture medium composition

expand / minimize

[Ref.: #18296]

Culture medium

ISP 4

[Ref.: #18296]

Culture medium growth

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Culture medium composition

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Culture medium

ISP 5

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Culture medium growth

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Culture medium composition

expand / minimize

[Ref.: #18296]

Culture medium

ISP 6

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Culture medium growth

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Culture medium composition

expand / minimize

[Ref.: #18296]

Culture medium

ISP 7

[Ref.: #18296]

Culture medium growth

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Culture medium composition

expand / minimize

[Ref.: #36447]

Culture medium

MEDIUM 566- Reasoner's 2A agar for Flavobacterium micromati

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Culture medium growth

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Culture medium composition

expand / minimize

[Ref.: #117208]

Culture medium

CIP Medium 566

[Ref.: #117208]

Culture medium growth

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Culture medium link

Medium recipe provided by DSMZ

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Culture medium

CIP Medium 328

[Ref.: #117208]

Culture medium growth

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Culture medium link

Medium recipe provided by DSMZ

[Ref.: #117208]

Culture medium

CIP Medium 72

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Culture medium growth

[Ref.: #117208]

Culture medium link

Medium recipe provided by DSMZ

		Temperature
[Ref.: #6431]	growth	28 °C
[Ref.: #18296]	optimum	28 °C
[Ref.: #31342]	growth	15-37 °C
[Ref.: #31342]	optimum	29.5 °C
[Ref.: #36447]	growth	30 °C
[Ref.: #67770]	growth	28 °C

[Ref.: #6431]	Temperature range	mesophilic
[Ref.: #18296]	Temperature range	mesophilic
[Ref.: #31342]	Temperature range	mesophilic
[Ref.: #36447]	Temperature range	mesophilic
[Ref.: #67770]	Temperature range	mesophilic

Information on physiology and metabolism Physiology and metabolism

[Ref.: #31342]

Oxygen tolerance

aerobe

[Ref.: #31342]	Ability of spore formation	no
[Ref.: #69481]	Ability of spore formation	no Confidence in %99
[Ref.: #69480]	Ability of spore formation	no Confidence in %99.432

[Ref.: #31342]	Observation	aggregates in chains
[Ref.: #67770]	Observation	quinones: MK-9(H₂)

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #18296]	arabinose ChEBI	-
[Ref.: #18296]	cellulose ChEBI	-
[Ref.: #68379]	D-glucose ChEBI	+	fermentation	* from API Coryne
[Ref.: #68379]	D-mannitol ChEBI	-	fermentation	* from API Coryne
[Ref.: #68379]	D-ribose ChEBI	-	fermentation	* from API Coryne
[Ref.: #68379]	D-xylose ChEBI	-	fermentation	* from API Coryne
[Ref.: #68379]	esculin ChEBI	-	hydrolysis	* from API Coryne
[Ref.: #18296]	fructose ChEBI	+
[Ref.: #68379]	gelatin ChEBI	+	hydrolysis	* from API Coryne
[Ref.: #18296]	glucose ChEBI	+
[Ref.: #68379]	glycogen ChEBI	-	fermentation	* from API Coryne
[Ref.: #68379]	lactose ChEBI	-	fermentation	* from API Coryne
[Ref.: #18296]	mannitol ChEBI	-
[Ref.: #18296]	myo-inositol ChEBI	-
[Ref.: #68379]	nitrate ChEBI	-	reduction	* from API Coryne
[Ref.: #18296]	raffinose ChEBI	-
[Ref.: #18296]	rhamnose ChEBI	-
[Ref.: #18296]	sucrose ChEBI	+
[Ref.: #68379]	sucrose ChEBI	-	fermentation	* from API Coryne
[Ref.: #31342]	urea ChEBI	+	carbon source
[Ref.: #68379]	urea ChEBI	-	hydrolysis	* from API Coryne
[Ref.: #18296]	xylose ChEBI	-
	Only first 10 entries are displayed. Click here to see all.

	Enzyme	Enzyme activity	EC number
[Ref.: #31342]	acid phosphatase	+	3.1.3.2
[Ref.: #68382]	acid phosphatase	+	3.1.3.2	* from API zym
[Ref.: #31342]	alkaline phosphatase	+	3.1.3.1
[Ref.: #68382]	alkaline phosphatase	+	3.1.3.1	* from API zym, API Coryne
[Ref.: #68382]	alpha-chymotrypsin	-	3.4.21.1	* from API zym
[Ref.: #68382]	alpha-fucosidase	-	3.2.1.51	* from API zym
[Ref.: #68382]	alpha-galactosidase	-	3.2.1.22	* from API zym
[Ref.: #68382]	alpha-glucosidase	+	3.2.1.20	* from API zym, API Coryne
[Ref.: #68382]	alpha-mannosidase	-	3.2.1.24	* from API zym
[Ref.: #68382]	beta-galactosidase	+	3.2.1.23	* from API zym, API Coryne
[Ref.: #68382]	beta-glucosidase	-	3.2.1.21	* from API zym, API Coryne
[Ref.: #68382]	beta-glucuronidase	-	3.2.1.31	* from API zym, API Coryne
[Ref.: #31342]	catalase	+	1.11.1.6
[Ref.: #68382]	cystine arylamidase	-	3.4.11.3	* from API zym
[Ref.: #68382]	esterase (C 4)	+		* from API zym
[Ref.: #68382]	esterase lipase (C 8)	+		* from API zym
[Ref.: #68379]	gelatinase	+		* from API Coryne
[Ref.: #68382]	leucine arylamidase	+	3.4.11.1	* from API zym
[Ref.: #68382]	lipase (C 14)	+		* from API zym
[Ref.: #68382]	N-acetyl-beta-glucosaminidase	-	3.2.1.52	* from API zym, API Coryne
[Ref.: #68382]	naphthol-AS-BI-phosphohydrolase	+		* from API zym
[Ref.: #68379]	pyrazinamidase	+	3.5.1.B15	* from API Coryne
[Ref.: #68379]	pyrrolidonyl arylamidase	+	3.4.19.3	* from API Coryne
[Ref.: #68382]	trypsin	-	3.4.21.4	* from API zym
[Ref.: #31342]	urease	+	3.5.1.5
[Ref.: #68379]	urease	-	3.5.1.5	* from API Coryne
[Ref.: #68382]	valine arylamidase	+		* from API zym
	Only first 10 entries are displayed. Click here to see all.

API® Coryne:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Reduction of nitrateNIT	PyrazinamidasePYZ	Pyrrolidonyl arylamidasePYRA	Alkaline phosphatasePAL	beta-Glucuronidasebeta GUR	beta-Galactosidasebeta GAL	alpha-Glucosidasealpha GLU	N-Acetyl-beta-Glucosidasebeta NAG	Esculin hydrolysis/beta-GlucosidaseESC	Urease/urea hydrolysisURE	Gelatinase/gelatin hydrolysisGEL	Control fermentationControl	Fermentation of D-glucoseGLU	Fermentation of D-riboseRIB	Fermentation of D-xyloseXYL	Fermentation of D-mannitolMAN	Fermentation of maltoseMAL	Fermentation of lactoseLAC	Fermentation of sucroseSAC	Fermentation of glycogenGLYG	CatalaseCAT
[Ref.: #18296]	4	-	+	+	+	-	+	+	-	-	-	+	not determinedn.d.	+	-	-	-	+/-	-	-	-	not determinedn.d.

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #18296]	5	not determinedn.d.	+	+	+	+	+	+	-	-	-	+	+	-	+	-	+	-	-	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #6431]	Sample type/isolated from	Mural painting, biofilm
[Ref.: #6431]	Geographic location (country and/or sea, region)	Herberstein
[Ref.: #6431]	Country	Austria
[Ref.: #6431]	Country ISO 3 Code	AUT
[Ref.: #6431]	Continent	Europe

[Ref.: #67770]	Sample type/isolated from	Rosy biofilm overgrowing a mural painting in the Saint-Catherine chapel
[Ref.: #67770]	Geographic location (country and/or sea, region)	castle of Herberstein
[Ref.: #67770]	Country	Austria
[Ref.: #67770]	Country ISO 3 Code	AUT
[Ref.: #67770]	Continent	Europe

[Ref.: #117208]	Sample type/isolated from	Mural painting
[Ref.: #117208]	Geographic location (country and/or sea, region)	Herberstein Castle, Himsdorf
[Ref.: #117208]	Country	Austria
[Ref.: #117208]	Country ISO 3 Code	AUT
[Ref.: #117208]	Continent	Europe
* marker position based on {}

Isolation sources categories

#Engineered	#Built environment	-
#Engineered	#Other	#Painting
#Environmental	#Biofilm	-

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence AJ639826 (>99% sequence identity) for Arthrobacter castelli from Microbeatlas

Aquatic Samples	253
Soil Samples	114
Animal Samples	390
Plant Samples	24
Total Samples	781

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #18296]	Biosafety level	2	Hazard group
[Ref.: #6431]	Biosafety level	1	Risk group (German classification) According to TRBA 466
[Ref.: #117208]	Biosafety level	1	Risk group (French classification)

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #6431]	Arthrobacter castelli partial 16S rRNA gene, type strain LMG 22283T	AJ639826	1509	ENA	271431 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #67770]	Arthrobacter castelli DSM 16402	GCA_000430705	scaffold	GenBank	1121019 tax ID
[Ref.: #66792]	Arthrobacter castelli DSM 16402	1121019.4	wgs	PATRIC	1121019 tax ID	*
[Ref.: #66792]	Arthrobacter castelli DSM 16402	2524023227	draft	JGI IMG/M	1121019 tax ID	*

[Ref.: #6431]	GC-content	68.1 mol%
[Ref.: #67770]	GC-content	63.6 mol%	genome sequence analysis
[Ref.: #67770]	GC-content	68.1 mol%	high performance liquid chromatography (HPLC)

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Arthrobacter castelli DSM 16402 DSM 16402 NCBI: GCA_000430705.1
[Ref.: #69481]	spore-forming	no	99	no

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Arthrobacter castelli DSM 16402 PATRIC: 1121019.4
[Ref.: #69480]	motile	no	90.342	no
[Ref.: #69480]	flagellated	no	97.526	no
[Ref.: #69480]	gram-positive	yes	93.348	yes
[Ref.: #69480]	anaerobic	no	99.557	no
[Ref.: #69480]	aerobic	yes	95.843	no
[Ref.: #69480]	halophile	yes	55.718	no
[Ref.: #69480]	spore-forming	no	84.82	yes
[Ref.: #69480]	thermophile	no	98.251	yes
[Ref.: #69480]	glucose-util	yes	88.055	yes
[Ref.: #69480]	glucose-ferment	no	86.973	no

Availability in culture collections External links

[Ref.: #6431]

Culture collection no.

DSM 16402, LMG 22283, JCM 21794, IAM 15349, CIP 108896, LMG 19502

[Ref.: #77047]

SI-ID 132113

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Six novel Arthrobacter species isolated from deteriorated mural paintings.	Heyrman J, Verbeeren J, Schumann P, Swings J, De Vos P	Int J Syst Evol Microbiol	10.1099/ijs.0.63358-0	2005	*

References

#6431

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 16402

#18296

Wink, J.: Compendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig .

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#31342

Barberan A, Caceres Velazquez H, Jones S, Fierer N.: Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information. mSphere 2: 2017 ( DOI 10.1128/mSphere.00237-17 , PubMed 28776041 ) - originally annotated from #27656 (see below)

#36447 ; Curators of the CIP;

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#68379

Automatically annotated from API Coryne .

#68382

Automatically annotated from API zym .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#77047

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID132113.1 )

#117208 Collection of Institut Pasteur ; Curators of the CIP; CIP 108896

#27656

IJSEM 1457 2005 ( DOI 10.1099/ijs.0.63358-0 , PubMed 16014466 )

* These data were automatically processed and therefore are not curated

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Arthrobacter castelli DSM 16402 DSM 16402

Arthrobacter castelli DSM 16402

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