Strain identifier

BacDive ID: 7603

Type strain: Yes

Species: Arthrobacter castelli

Strain Designation: R-5102

Culture col. no.: DSM 16402, LMG 22283

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General

@ref: 6431

BacDive-ID: 7603

DSM-Number: 16402

keywords: gram-positive, mesophilic, aerobe, Bacteria, 16S sequence, genome sequence, rod-shaped

description: Arthrobacter castelli R-5102 is an aerobe, mesophilic, gram-positive bacterium that was isolated from Mural painting, biofilm.

strain history

doi: 10.13145/bacdive7603.20201210.5

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinobacteria
  • domain: Bacteria
  • phylum: Actinobacteria
  • class: Actinobacteria
  • order: Micrococcales
  • family: Micrococcaceae
  • genus: Arthrobacter
  • species: Arthrobacter castelli
  • full scientific name: Arthrobacter castelli Heyrman et al. 2005

@ref: 6431

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Micrococcaceae

genus: Arthrobacter

species: Arthrobacter castelli

full scientific name: Arthrobacter castelli Heyrman et al. 2005 emend. Nouioui et al. 2018

strain designation: R-5102

type strain: yes

Morphology

cell morphology

  • @ref: 31342
  • gram stain: positive
  • cell width: 0.9 µm
  • cell shape: rod-shaped
  • motility: no

colony morphology

@refincubation periodcolony colormedium used
64318-14 days
1829610-14 daysHoney yellow (1005)ISP 2
1829610-14 daysHoney yellow (1005)ISP 3
1829610-14 daysCream (9001)ISP 4
1829610-14 daysHoney yellow (1005)ISP 5
1829610-14 daysLemon yellow (1012)ISP 6
1829610-14 daysLemon yellow (1012)ISP 7

multicellular morphology

@refforms multicellular complexmedium name
18296noISP 2
18296noISP 3
18296noISP 4
18296noISP 5
18296noISP 6
18296noISP 7

pigmentation

  • @ref: 31342
  • production: no

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
6431BACTO MARINE BROTH (DIFCO 2216) (DSMZ Medium 514)yeshttps://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium514.pdf
18296ISP 2yesName: ISP 2 / Yeast Malt Agar (5265); 5265 Composition Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l Agar 15.0 g/l Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.0 Usage: Maintenance and Taxonomy Organisms: All Actinomycetes
18296ISP 3yesName: ISP 3; 5315 Composition Dog oat flakes 20.0 g/l Trace element solution (5314) 2.5 ml/l Agar 18.0 g/l Preparation: Oat flakes are cooked for 20 minutes, trace element solution and agar are added (in the case of non rolled oat flakes the suspension has to bee filtrated). Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.8 Usage: Maintenance and taxonomy (e.g. SEM As liquid medium for metabolite production) Organisms: All Actinomycetes Trace element solution 5314 Name: Trace element solution 5314; 5314 Composition CaCl2 x H2O 3.0 g/l Fe-III-citrate 1.0 g/l MnSO4 0.2 g/l ZnCl2 0.1 g/l CuSO4 x 5 H2O 0.025 g/l Sodium tetra borate 0.2 g/l CoCl2 x 6 H2O 0.004 g/l Sodium molybdate 0.01 g/l Preparation: Use double destillated water. Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Trace element solution for different media Organisms:
18296ISP 4yesName: ISP 4; DSM 547 Solution I: Difco soluble starch, 10.0 g. Make a paste of the starch with a small amount of cold distilled water and bring to a volume of 500 ml. Solution II: CaCO3 2.0 g K2HPO4 (anhydrous) 1.0 g MgSO4 x 7 H2O 1.0 g NaCl 1.0 g (NH4)2SO4 2.0 g Distilled water 500.0 ml Trace salt solution (see below) 1.0 ml The pH should be between 7.0 and 7.4. Do not adjust if it is within this range. Mix solutions I and II together. Add 20.0 g agar. Liquify agar by steaming at 100°C for 10 to 20 min. Trace element solution: FeSO4 x 7 H2O 0.1 g MnCl2 x 4 H2O 0.1 g ZnSO4 x 7 H2O 0.1 g Distilled water 100.0 ml
18296ISP 5yesName: ISP 5 (5323) Composition L-Asparagine 1.0 g/l Glycerol 10.0 g/l K2HPO4 1.0 g/l Salt solution (see preparation) 1.0 ml/l Agar 20.0 g/l Preparation: Salt solution 1.0 g FeSO4 x 7 H2O 1.0 g MnCl2 x 4 H2O 1.0 g ZNSO4 x 7 H2O in 100 ml water Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.2 Usage: Maintenance and taxonomy Organisms: All Actinomycetes
18296ISP 6yesName: ISP 6 (5318) Composition Peptone 15.0 g/l Proteose peptose 5.0 g/l Ferric ammonium citrate 0.5 g/l Sodium glycerophosphate 1.0 g/l Sodium thiosulfate 0.08 g/l Yeast extract 1.0 g/l Agar 15.0 g/l Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Production of melanoid pigments Organisms: All Actinomycetes
18296ISP 7yesName: ISP 7 (5322) Composition Glycerol 15.0 g/l L-Tyrosine 0.5 g/l L-Asparagine 1.0 g/l K2HPO4 0.5 g/l NaCl 0.5 g/l FeSO4 x 7 H2O 0.01 g/l Trace element solution 5343 1.0 ml/l Agar 20.0 Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.3 Usage: Production of melanoid pigments Organisms: All Actinomycetes
36447MEDIUM 566- Reasoner's 2A agar for Flavobacterium micromatiyesDistilled water make up to (1000.000 ml);R2A agar (18.200 g)

culture temp

@refgrowthtypetemperaturerange
18296positiveoptimum28mesophilic
6431positivegrowth28mesophilic
31342positivegrowth15-37
31342positiveoptimum29.5mesophilic
36447positivegrowth30mesophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 31342
  • oxygen tolerance: aerobe

spore formation

  • @ref: 31342
  • spore formation: no

observation

  • @ref: 31342
  • observation: aggregates in chains

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
1829662968cellulose-
1829616634raffinose-
1829626546rhamnose-
1829628757fructose+
1829629864mannitol-
1829617268myo-inositol-
1829618222xylose-
1829617992sucrose+
1829622599arabinose-
1829617234glucose+
3134216199urea+carbon source

enzymes

@refvalueactivityec
31342acid phosphatase+3.1.3.2
31342alkaline phosphatase+3.1.3.1
31342catalase+1.11.1.6
31342urease+3.5.1.5

API coryne

@refNITPYZPYRAPALbeta GURbeta GALalpha GLUbeta NAGESCUREGELGLURIBXYLMANMALLACSACGLYG
18296-+++-++---++---+/----

API zym

@refAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
18296++++++---++-+-+----

Isolation, sampling and environmental information

isolation

  • @ref: 6431
  • sample type: Mural painting, biofilm
  • geographic location: Herberstein
  • country: Austria
  • origin.country: AUT
  • continent: Europe

isolation source categories

Cat1Cat2Cat3
#Engineered#Built environment
#Engineered#Other#Painting
#Environmental#Biofilm

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
182962Hazard group
64311Risk group (German classification)

Sequence information

16S sequences

  • @ref: 6431
  • description: Arthrobacter castelli partial 16S rRNA gene, type strain LMG 22283T
  • accession: AJ639826
  • length: 1509
  • database: ena
  • NCBI tax ID: 271431

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Arthrobacter castelli DSM 16402GCA_000430705scaffoldncbi1121019
66792Arthrobacter castelli DSM 164021121019.4wgspatric1121019
66792Arthrobacter castelli DSM 164022524023227permanentimg1121019

GC content

  • @ref: 6431
  • GC-content: 68.1

External links

@ref: 6431

culture collection no.: DSM 16402, LMG 22283

straininfo link

@refpassport
20218http://www.straininfo.net/strains/381620
20218http://www.straininfo.net/strains/378627

Reference

@idauthorscataloguedoi/urltitlejournalpubmedID_cross_reference
6431Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 16402)https://www.dsmz.de/collection/catalogue/details/culture/DSM-16402
18296Wink, J.http://www.dsmz.de/microorganisms/wink_pdf/DSM16402.pdfCompendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig
20215D.Gleim, M.Kracht, N.Weiss et. al.http://www.dsmz.de/bacterial-diversity/prokaryotic-nomenclature-up-to-date.htmlProkaryotic Nomenclature Up-to-date - compilation of all names of Bacteria and Archaea, validly published according to the Bacteriological Code since 1. Jan. 1980, and validly published nomenclatural changes since
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
2765610.1099/ijs.0.63358-0
31342Barberan A, Caceres Velazquez H, Jones S, Fierer N.10.1128/mSphere.00237-17Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information27656
36447Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/6577
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)