Bacillus pumilus (DSM 361, ATCC 14884, CCM 2218)

Name: Bacillus pumilus (DSM 361, ATCC 14884, CCM 2218)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 751

Type strain

Strain Designation CIP3.83, Mill Hill

Culture col. no. DSM 361 ATCC 14884 CCM 2218 CIP 3.83 IFO 12102 10 more

NCBI tax ID(s) 1408

Special Collections DSMZ CCUG CIP

History <- NCTC <- J.H. Humphrey CIP <- 1976, NCTC <- J.H. Humphrey: strain Mill Hill

Links

Bacillus pumilus CIP3.83 is an aerobe, spore-forming, mesophilic prokaryote of the family Bacillaceae.

spore-forming Gram-positive motile rod-shaped aerobe mesophilic 16S sequence

Name and taxonomic classification

SI-ID 12135

LMG 18517,ATCC 14884,CCM 2218,IFO 12102,NCIB 8982,NCTC 8241,CCUG 3273,IFO 3813,NCIMB 8982,IMET10631,CIP 76.18,CIP 3.83,CECT 152,CCRC 11597,NBRC 3813,NBRC 12102,BCRC 11597,BCRC 16083,CCRC 16083,CCT 2487,NCAIM B.01717,NCAIM B.01880,NCIM 2327,CNCTC 6214,CGMCC 1.2422,DSM 361

@ref 20215

Last LPSN update 2025-12-16 09:46:56

Domain Bacillati

Phylum Bacillota

Class Bacilli

Order Caryophanales

Family Bacillaceae

Genus Bacillus

Species Bacillus pumilus

Full scientific name Bacillus pumilus Meyer and Gottheil 1901 (Approved Lists 1980)

BacDive ID	Other strains from **Bacillus pumilus** (72)	Type strain
756	B. pumilus 272, DSM 27, ATCC 7061, CCM 2144, IFO 12092, ... (type strain)
749	B. pumilus 734a, 735, DSM 354, ATCC 71, CCM 1726
750	B. pumilus 734, 736, DSM 355, ATCC 72, CCM 1697, IFO ...
752	B. pumilus E 601, E601, CCUG 24142, DSM 492, ATCC 27142, ...
753	B. pumilus 29, DSM 766
754	B. pumilus DSM 1794, NCIB 10822, CN 607
755	B. pumilus 236, 726, DSM 2893, ATCC 6631, IFO 12110, ...
757	B. pumilus G1R, DSM 5549, HER 1182
758	B. pumilus DSM 8786, DSM 46309, IMET 11026, NCIB 7198
759	B. pumilus ES 20, DSM 13835
760	B. pumilus ES1-184-2, DSM 30537
761	B. pumilus ES1-215-1, DSM 30550
762	B. pumilus ES2-8a2, DSM 30596
763	B. pumilus ES2-12c1, DSM 30606
764	B. pumilus FR2-W6Ac, DSM 30704
765	B. pumilus FR2-W7Aa, DSM 30708
766	B. pumilus FR2-W7Ab, DSM 30709
767	B. pumilus FR2-W8Aa, DSM 30714
768	B. pumilus W17/3, DSM 30786
769	B. pumilus ES2-W4c1, DSM 30628
770	B. pumilus FR2-S1Aa, DSM 30670
771	B. pumilus FR2-S4Aa, DSM 30672
772	B. pumilus FR2-S4Ab, DSM 30673
773	B. pumilus FR2-S7Ab, DSM 30678
774	B. pumilus FR2-S7Ac, DSM 30679
775	B. pumilus FR2-S11Aa-b, DSM 30681
776	B. pumilus FR2-W6Ab, DSM 30703
777	B. pumilus FR1-11con, DSM 30720
778	B. pumilus W20/5, DSM 30789
779	B. pumilus MW15/1, DSM 30805
780	B. pumilus TS1/1, DSM 30806
781	B. pumilus KO_PS 3, DSM 30833
130862	B. pumilus W2, DSM 100617
130861	B. pumilus T4_3, DSM 100541
22913	B. pumilus DSM 30004, ATCC 15716, DSM 15573, IFO 12605, ...
130168	B. pumilus b17_1, DSM 31005
130169	B. pumilus T2_3, DSM 30977
130170	B. pumilus T2_44, DSM 30997
131630	B. pumilus Stev13, DSM 103049
131631	B. pumilus Stev38, DSM 103053
131632	B. pumilus D35_1, DSM 103757
131633	B. pumilus C8_12, DSM 103869
134937	B. pumilus 7.87, CIP 102756
137078	B. pumilus CIP 51.18, CN 2731
137576	B. pumilus 520.86, CIP 102496
138631	B. pumilus CIP 52.42
138682	B. pumilus H 634, CIP 101996
138686	B. pumilus CIP 102081
139688	B. pumilus T3_14, DSM 103904
141248	B. pumilus CCUG 397
141663	B. pumilus CCUG 4626
143929	B. pumilus CCUG 21924
144775	B. pumilus CCUG 26531
144948	B. pumilus CCUG 27243
144950	B. pumilus CCUG 27263
145355	B. pumilus CCUG 28267 B
146007	B. pumilus CCUG 30128
146237	B. pumilus CCUG 30686
147025	B. pumilus CCUG 32666
147795	B. pumilus CCUG 34244
148189	B. pumilus CCUG 35011
150505	B. pumilus CCUG 41443
151344	B. pumilus CCUG 43804
151785	B. pumilus CCUG 44877
151802	B. pumilus CCUG 44940
152656	B. pumilus CCUG 47302
153625	B. pumilus CCUG 50373 A
155159	B. pumilus CCUG 56623
156911	B. pumilus CCUG 66887
173512	B. pumilus CIMB23, CIMB 05-1841, CRBIP17.23
173513	B. pumilus CIMB 05-0968, CRBIP17.25
176402	B. pumilus 45.39, DSM 117807

Morphology

Cell morphology

@ref	Gram stain	Cell shape	Motility
122059	positive	rod-shaped

Culture and growth conditions

Culture medium

@ref	Name	Medium link	Composition
151	NUTRIENT AGAR (DSMZ Medium 1)	Medium recipe at MediaDive	Name: NUTRIENT AGAR (DSMZ Medium 1) Composition: Agar 15.0 g/l Peptone 5.0 g/l Meat extract 3.0 g/l Distilled water
36568	MEDIUM 72- for trypto casein soja agar		Distilled water make up to (1000.000 ml);Trypto casein soy agar (40.000 g)
122059	CIP Medium 72	Medium recipe at CIP

Culture temp

@ref	Growth	Type	Temperature (°C)	Range
151	positive	growth	30	mesophilic
36568	positive	growth	30	mesophilic
44628	positive	growth	25-42
122059	positive	growth	30-45
122059	negative	growth	10
122059	negative	growth	55

Culture pH

@ref	Ability	Type	PH
122059	positive	growth	6

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance
44628	aerobe
122059	obligate aerobe

Antibiotic susceptibility testing

	Test 1	Test 2
@ref	151	151
Medium	Mueller-Hinton Agar	Mueller-Hinton Agar
Manual annotation	1	1
	Inhibition zone diameter in mm	Inhibition zone diameter in mm
Amikacin 30µg (disc)	28-30	30
Ampicillin 10µg (disc)	38	38
Aztreonam 30µg (disc)	0	8-10
Bacitracin 10Unit	6	6
Cefalotin 30µg (disc)	46	40-42
Cefazolin 30µg (disc)	40	38
Cefotaxime 30µg (disc)	18-20	14
Ceftriaxone 30µg (disc)	28-30	n.d.
Chloramphenicol 30µg (disc)	26	24
Clindamycin 10µg (disc)	24-26	n.d.
Colistin 10µg (disc)	10	10
Doxycycline 30µg (disc)	40	34-36
Erythromycin 15µg (disc)	32	30
Fosfomycin 50µg (disc)	18-20	n.d.
Gentamycin 10µg (disc)
Imipenem 10µg (disc)	46	44
Kanamycin 30µg (disc)	30	30
Lincomycin 15µg (disc)	0	10
Linezolid 10µg (disc)	40	n.d.
Mezlocillin 30µg (disc)	36	32-34
Moxifloxacin 5µg (disc)	40	n.d.
Neomycin 30µg (disc)	26	24-26
Nitrofurantoin 100µg (disc)	26	24
Norfloxacin 10µg (disc)	28	28-30
Nystatin 100Unit	0	n.d.
Ofloxacin 5µg (disc)	34	32
Oxacillin 5µg (disc)	30	28-30
Penicillin G 6µg (disc)	40	40
Pipemidic acid 20µg (disc)	16	18
Piperacillin/Tazobactam 40µg (disc)	34	n.d.
Polymyxin B 300Unit	14	14
Quinupristin/Dalfopristin 15µg (disc)	26	n.d.
Teicoplanin 30µg (disc)	22	n.d.
Tetracycline 30µg (disc)	36	32
Ticarcillin 75µg (disc)	44	44
Vancomycin 30µg (disc)	26	22-24

Spore formation

122059

Spore formationyes

Halophily

@ref	Salt	Growth	Tested relation	Concentration
122059	NaCl	positive	growth	0-10 %

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
68371	27613 ChEBI	amygdalin	-	builds acid from	from API 50CH acid
68368	27613 ChEBI	amygdalin	-	fermentation	from API 20E
68371	18305 ChEBI	arbutin	+	builds acid from	from API 50CH acid
68368	29016 ChEBI	arginine	-	hydrolysis	from API 20E
68371	17057 ChEBI	cellobiose	+	builds acid from	from API 50CH acid
122059	16947 ChEBI	citrate	-	carbon source
68368	16947 ChEBI	citrate	+	assimilation	from API 20E
68371	17108 ChEBI	D-arabinose	-	builds acid from	from API 50CH acid
68371	18333 ChEBI	D-arabitol	-	builds acid from	from API 50CH acid
68371	15824 ChEBI	D-fructose	+	builds acid from	from API 50CH acid
68371	28847 ChEBI	D-fucose	-	builds acid from	from API 50CH acid
68371	12936 ChEBI	D-galactose	-	builds acid from	from API 50CH acid
68371	17634 ChEBI	D-glucose	+	builds acid from	from API 50CH acid
68368	17634 ChEBI	D-glucose	-	fermentation	from API 20E
68371	62318 ChEBI	D-lyxose	-	builds acid from	from API 50CH acid
68379	16899 ChEBI	D-mannitol	+	fermentation	from API Coryne
68371	16899 ChEBI	D-mannitol	+	builds acid from	from API 50CH acid
68368	16899 ChEBI	D-mannitol	-	fermentation	from API 20E
68371	16024 ChEBI	D-mannose	+	builds acid from	from API 50CH acid
68379	16988 ChEBI	D-ribose	+	fermentation	from API Coryne
68371	16988 ChEBI	D-ribose	+	builds acid from	from API 50CH acid
68371	17924 ChEBI	D-sorbitol	-	builds acid from	from API 50CH acid
68371	16443 ChEBI	D-tagatose	+	builds acid from	from API 50CH acid
68379	65327 ChEBI	D-xylose	-	fermentation	from API Coryne
68371	65327 ChEBI	D-xylose	-	builds acid from	from API 50CH acid
68371	17113 ChEBI	erythritol	-	builds acid from	from API 50CH acid
68379	4853 ChEBI	esculin	+	hydrolysis	from API Coryne
122059	4853 ChEBI	esculin	+	hydrolysis
68371	4853 ChEBI	esculin	+	builds acid from	from API 50CH acid
68371	16813 ChEBI	galactitol	-	builds acid from	from API 50CH acid
68379	5291 ChEBI	gelatin	+	hydrolysis	from API Coryne
68368	5291 ChEBI	gelatin	+	hydrolysis	from API 20E
68371	28066 ChEBI	gentiobiose	+	builds acid from	from API 50CH acid
68371	24265 ChEBI	gluconate	-	builds acid from	from API 50CH acid
68371	17754 ChEBI	glycerol	-	builds acid from	from API 50CH acid
68379	28087 ChEBI	glycogen	-	fermentation	from API Coryne
68371	28087 ChEBI	glycogen	-	builds acid from	from API 50CH acid
122059	606565 ChEBI	hippurate	-	hydrolysis
68371	15443 ChEBI	inulin	-	builds acid from	from API 50CH acid
68371	30849 ChEBI	L-arabinose	+	builds acid from	from API 50CH acid
68368	30849 ChEBI	L-arabinose	-	fermentation	from API 20E
68371	18403 ChEBI	L-arabitol	-	builds acid from	from API 50CH acid
68371	18287 ChEBI	L-fucose	-	builds acid from	from API 50CH acid
68371	62345 ChEBI	L-rhamnose	-	builds acid from	from API 50CH acid
68368	62345 ChEBI	L-rhamnose	-	fermentation	from API 20E
68371	17266 ChEBI	L-sorbose	-	builds acid from	from API 50CH acid
68371	65328 ChEBI	L-xylose	-	builds acid from	from API 50CH acid
68379	17716 ChEBI	lactose	-	fermentation	from API Coryne
68371	17716 ChEBI	lactose	-	builds acid from	from API 50CH acid
68368	25094 ChEBI	lysine	-	degradation	from API 20E
68379	17306 ChEBI	maltose	-	fermentation	from API Coryne
68371	17306 ChEBI	maltose	-	builds acid from	from API 50CH acid
68371	6731 ChEBI	melezitose	-	builds acid from	from API 50CH acid
68371	28053 ChEBI	melibiose	-	builds acid from	from API 50CH acid
68368	28053 ChEBI	melibiose	-	fermentation	from API 20E
68371	320061 ChEBI	methyl alpha-D-glucopyranoside	-	builds acid from	from API 50CH acid
68371	43943 ChEBI	methyl alpha-D-mannoside	-	builds acid from	from API 50CH acid
68371	74863 ChEBI	methyl beta-D-xylopyranoside	-	builds acid from	from API 50CH acid
68371	17268 ChEBI	myo-inositol	-	builds acid from	from API 50CH acid
68368	17268 ChEBI	myo-inositol	-	fermentation	from API 20E
68371	59640 ChEBI	N-acetylglucosamine	+	builds acid from	from API 50CH acid
68379	17632 ChEBI	nitrate	-	reduction	from API Coryne
122059	17632 ChEBI	nitrate	-	reduction
122059	17632 ChEBI	nitrate	-	respiration
122059	16301 ChEBI	nitrite	-	reduction
68368	18257 ChEBI	ornithine	-	degradation	from API 20E
68371		Potassium 2-ketogluconate	-	builds acid from	from API 50CH acid
68371		Potassium 5-ketogluconate	-	builds acid from	from API 50CH acid
68371	16634 ChEBI	raffinose	-	builds acid from	from API 50CH acid
68371	15963 ChEBI	ribitol	-	builds acid from	from API 50CH acid
68371	17814 ChEBI	salicin	+	builds acid from	from API 50CH acid
68368	30911 ChEBI	sorbitol	-	fermentation	from API 20E
68371	28017 ChEBI	starch	-	builds acid from	from API 50CH acid
68379	17992 ChEBI	sucrose	+	fermentation	from API Coryne
68371	17992 ChEBI	sucrose	+	builds acid from	from API 50CH acid
68368	17992 ChEBI	sucrose	-	fermentation	from API 20E
68371	27082 ChEBI	trehalose	+	builds acid from	from API 50CH acid
68368	27897 ChEBI	tryptophan	-	energy source	from API 20E
68371	32528 ChEBI	turanose	-	builds acid from	from API 50CH acid
68379	16199 ChEBI	urea	-	hydrolysis	from API Coryne
68368	16199 ChEBI	urea	-	hydrolysis	from API 20E
68371	17151 ChEBI	xylitol	-	builds acid from	from API 50CH acid

Antibiotic resistance

@ref	ChEBI	Metabolite	Is resistant	Resistance conc.
151	7660	Nystatin		100 Unit	from Antibiotic test

Metabolite production

@ref	Chebi-ID	Metabolite
68368	15688 ChEBI	acetoin	from API 20E
68368	16136 ChEBI	hydrogen sulfide	from API 20E
122059	35581 ChEBI	indole
68368	35581 ChEBI	indole	from API 20E

Metabolite tests

@ref	Chebi-ID	Metabolite	Voges-proskauer-test	Methylred-test	Indole test
122059	15688 ChEBI	acetoin	+
68368	15688 ChEBI	acetoin	+			from API 20E
122059	17234 ChEBI	glucose		+
68368	35581 ChEBI	indole			-	from API 20E

Enzymes

@ref	Value	Activity	Ec
68382	acid phosphatase	+	3.1.3.2	from API zym
122059	alcohol dehydrogenase	+	1.1.1.1
68382	alkaline phosphatase	+	3.1.3.1	from API zym
68379	alkaline phosphatase	+	3.1.3.1	from API Coryne
68382	alpha-chymotrypsin	+	3.4.21.1	from API zym
68382	alpha-fucosidase	-	3.2.1.51	from API zym
68382	alpha-galactosidase	-	3.2.1.22	from API zym
68382	alpha-glucosidase	+	3.2.1.20	from API zym
68379	alpha-glucosidase	+	3.2.1.20	from API Coryne
68382	alpha-mannosidase	+	3.2.1.24	from API zym
122059	amylase	-
68368	arginine dihydrolase	-	3.5.3.6	from API 20E
122059	beta-galactosidase	+	3.2.1.23
68382	beta-galactosidase	+	3.2.1.23	from API zym
68379	beta-galactosidase	+	3.2.1.23	from API Coryne
68368	beta-galactosidase	+	3.2.1.23	from API 20E
68382	beta-glucosidase	+	3.2.1.21	from API zym
68379	beta-glucosidase	+	3.2.1.21	from API Coryne
68382	beta-glucuronidase	-	3.2.1.31	from API zym
68379	beta-glucuronidase	-	3.2.1.31	from API Coryne
122059	caseinase	-	3.4.21.50
122059	catalase	+	1.11.1.6
68379	catalase	+	1.11.1.6	from API Coryne
68382	cystine arylamidase	-	3.4.11.3	from API zym
122059	DNase	-
68382	esterase (C 4)	+		from API zym
68382	esterase lipase (C 8)	+		from API zym
122059	gamma-glutamyltransferase	+	2.3.2.2
122059	gelatinase	+
68379	gelatinase	+		from API Coryne
68368	gelatinase	+		from API 20E
122059	lecithinase	-
68382	leucine arylamidase	+	3.4.11.1	from API zym
122059	lipase	-
68382	lipase (C 14)	-		from API zym
122059	lysine decarboxylase	-	4.1.1.18
68368	lysine decarboxylase	-	4.1.1.18	from API 20E
68382	N-acetyl-beta-glucosaminidase	-	3.2.1.52	from API zym
68379	N-acetyl-beta-glucosaminidase	-	3.2.1.52	from API Coryne
68382	naphthol-AS-BI-phosphohydrolase	-		from API zym
122059	ornithine decarboxylase	-	4.1.1.17
68368	ornithine decarboxylase	-	4.1.1.17	from API 20E
122059	oxidase	+
122059	protease	+
68379	pyrazinamidase	+	3.5.1.B15	from API Coryne
68379	pyrrolidonyl arylamidase	-	3.4.19.3	from API Coryne
68382	trypsin	-	3.4.21.4	from API zym
68368	tryptophan deaminase	-	4.1.99.1	from API 20E
122059	tween esterase	-
122059	urease	-	3.5.1.5
68379	urease	-	3.5.1.5	from API Coryne
68368	urease	-	3.5.1.5	from API 20E
68382	valine arylamidase	-		from API zym

Fatty acid profile

Metadata FA analysis

@ref

44628

Fatty acid	Percentage	ECL
C15:0 ISO	47.9	14.621
C15:0 ANTEISO	26.8	14.711
C17:1 ω10c ISO	4.1	16.387
C17:0 iso	3.3	16.629
C17:0 anteiso	3.1	16.722
C16:1 ω11c	2.9	15.757
C11:0 3OH	2.5	12.441
C16:1 ω7c alcohol	1.6	15.386
C16:0	1.5	16
C17:1 ISO I/C16:0 DMA	1.5	16.481
C16:0 iso	1.3	15.626
C14:0 ISO	1.2	13.618
C18:1 ω9c	0.8	17.769
C13:0 iso	0.7	12.612
C14:0	0.6	14
C18:2 ω6,9c/C18:0 ANTE	0.4	17.724

API 20E

@ref	ONPG	ADH (Arg)	LDC (Lys)	ODC	CIT	H2S productionH2S	URE	TDA (Trp)	IND	Acetoin production (Voges Proskauer test)VP	GEL	GLU	MAN	INO	Sor	RHA	SAC	MEL	AMY	ARA	OX	Nitrite productionNO2	Reduction to N2N2	MotilityMOB	Growth on MacConkey mediumMAC	OF-O	OF-F
44628	+	-	-	-	+	-	-	-	-	+	+	-	-	-	-	-	-	-	-	-	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.

API coryne

@ref	Reduction of nitrateNIT	PYZ	PYRA	PAL	beta GUR	beta GAL	alpha GLU	beta NAG	ESC	URE	GEL	Control fermentationControl	GLU	RIB	XYL	MAN	MAL	LAC	SAC	GLYG	CAT
44628	-	+	-	+	-	+	+	-	+	-	+	-	not determinedn.d.	+	-	+	-	-	+	-	+

API zym

@ref	Control	Alkaline phosphatase	Esterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	Lipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	Trypsin	alpha- Chymotrypsin	Acid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	alpha- Galactosidase	beta- Galactosidase	beta- Glucuronidase	alpha- Glucosidase	beta- Glucosidase	N-acetyl-beta- glucosaminidase	alpha- Mannosidase	alpha- Fucosidase
122059	-	+	+	+	-	+	-	-	-	+	+	-	-	+	-	+	+	-	+	-

API 50CHac

@ref	ControlQ	GLY	ERY	DARA	LARA	RIB	DXYL	LXYL	ADO	MDX	GAL	GLU	FRU	MNE	SBE	RHA	DUL	INO	MAN	SOR	MDM	MDG	NAG	AMY	ARB	ESC	SAL	CEL	MAL	LAC	MEL	SAC	TRE	INU	MLZ	RAF	AMD	GLYG	XLT	GEN	TUR	LYX	TAG	DFUC	LFUC	DARL	LARL	GNT	2KG	5KG
44628	-	-	-	-	+	+	-	-	-	-	-	+	+	+	-	-	-	-	+	-	-	-	+	-	+	+	+	+	-	-	-	+	+	-	-	-	-	-	-	+	-	-	+	-	-	-	-	-	-	-

API biotype100

@ref	ControlQ	D-glucose as carbon sourceGLU	D-fructose as carbon sourceFRU	D-galactose as carbon sourceGAL	D-trehalose as carbon sourceTRE	D-mannose as carbon sourceMNE	L-sorbose as carbon sourceSBE	D-melibiose as carbon sourceMEL	sucrose as carbon sourceSAC	D-raffinose as carbon sourceRAF	maltotriose as carbon sourceMTE	maltose as carbon sourceMAL	lactose as carbon sourceLAC	lactulose as carbon sourceLTE	1-O-methyl-beta-galactopyranoside as carbon sourceMbGa	1-O-methyl-alpha-galactopyranoside as carbon sourceMaGa	D-cellobiose as carbon sourceCEL	gentiobiose as carbon sourceGEN	1-O-methyl-beta-D-glucopyranoside as carbon sourceMbGu	esculin as carbon sourceESC	D-ribose as carbon sourceRIB	L-arabinose as carbon sourceARA	D-xylose as carbon sourceXYL	palatinose as carbon sourcePLE	L-rhamnose as carbon sourceRHA	L-fucose as carbon sourceFUC	D-melezitose as carbon sourceMLZ	D-arabitol as carbon sourceDARL	L-arabitol as carbon sourceLARL	xylitol as carbon sourceXLT	dulcitol as carbon sourceDUL	D-tagatose as carbon sourceTAG	glycerol as carbon sourceGLY	myo-inositol as carbon sourceINO	D-mannitol as carbon sourceMAN	maltitol as carbon sourceMTL	D-turanose as carbon sourceTUR	D-sorbitol as carbon sourceSOR	adonitol as carbon sourceADO	hydroxyquinoline-beta-glucuronide as carbon sourceHBG	D-lyxose as carbon sourceLYX	i-erythritol as carbon sourceERY	1-O-methyl-alpha-D-glucoside as carbon sourceMDG	3-O-methyl-D-glucose as carbon source3MDG	D-saccharate as carbon sourceSAT	mucate as carbon sourceMUC	L-tartrate as carbon sourceLTAT	D-tartrate as carbon sourceDTAT	meso-tartrate as carbon sourceMTAT	D-malate as carbon sourceDMLT	L-malate as carbon sourceLMLT	cis-aconitate as carbon sourceCATE	trans-aconitate as carbon sourceTATE	tricarballylate as carbon sourceTTE	citrate as carbon sourceCIT	D-glucuronate as carbon sourceGRT	D-galacturonate as carbon sourceGAT	2-ketogluconate as carbon source2KG	5-ketogluconate as carbon source5KG	tryptophan as carbon sourceTRY	N-acetyl-D-glucosamine as carbon sourceNAG	D-gluconate as carbon sourceGNT	phenylacetate as carbon sourcePAC	protocatechuate as carbon sourcePAT	4-hydroxybenzoate as carbon sourcepOBE	quinate as carbon sourceQAT	gentisate as carbon sourceGTE	3-hydroxybenzoate as carbon sourcemOBE	benzoate as carbon sourceBAT	3-phenylpropionate as carbon sourcePPAT	m-coumarate as carbon sourceCMT	trigonelline as carbon sourceTGE	betaine as carbon sourceBET	putrescine as carbon sourcePCE	4-aminobutyrate as carbon sourceABT	histamine as carbon sourceHIN	DL-lactate as carbon sourceLAT	caprate as carbon sourceCAP	caprylate as carbon sourceCYT	L-histidine as carbon sourceHIS	succinate as carbon sourceSUC	fumarate as carbon sourceFUM	glutarate as carbon sourceGRE	DL-glycerate as carbon sourceGYT	5-aminovalerate as carbon sourceAVT	ethanolamine as carbon sourceETN	tryptamine as carbon sourceTTN	D-glucosamine as carbon sourceGLN	itaconate as carbon sourceITA	3-hydroxybutyrate as carbon source3OBU	L-aspartate as carbon sourceAPT	L-glutamate as carbon sourceGTT	L-proline as carbon sourcePRO	D-alanine as carbon sourceDALA	L-alanine as carbon sourceLALA	L-serine as carbon sourceSER	malonate as carbon sourceMNT	propionate as carbon sourcePROP	L-tyrosine as carbon sourceTYR	2-ketoglutarate as carbon source2KT
122059	not determinedn.d.	+	+	+	+	-	-	-	+	-	-	+	-	-	-	-	+	+	-	+	+	+	+	+	-	-	-	-	-	-	-	-	+	-	-	-	-	-	-	-	-	-	+	-	-	-	-	-	-	-	+	+	+	-	+	-	-	-	-	-	+	+	-	-	-	-	-	-	-	-	-	-	-	-	+	-	-	-	-	-	+	+	-	-	-	-	-	+	-	-	+	+	-	-	-	-	-	-	-	-

Isolation, sampling and environmental information

Interaction and safety

Risk assessment

@ref	Pathogenicity human	Biosafety level	Biosafety level comment
151	yes, in single cases	1	Risk group (German classification) According to TRBA 466
122059		1	Risk group (French classification) According to TRBA 466

Sequence information

16S sequences

@ref	Description	Accession	Length	NCBI tax ID
20218	Bacillus pumilus strain ATCC 14884 16S ribosomal RNA gene, partial sequence	JF749284	803	1408
124043	Bacillus pumilus strain ATCC 14884 16S ribosomal RNA gene, partial sequence.	MN456845	1551	1408
124043	Bacillus pumilus strain CIP 76.18 16S ribosomal RNA gene, partial sequence.	AY876046	1018	1408

GC content

151

GC-content (mol%)42.9-43.9

Genome-based predictions

Literature

Topic	Title	Authors	Journal	DOI	Year
	Quantitative Assessment of Antimicrobial Activity of PLGA Films Loaded with 4-Hexylresorcinol.	Kemme M, Heinzel-Wieland R.	J Funct Biomater	10.3390/jfb9010004	2018
Phylogeny	Chemotaxonomic identification of single bacteria by micro-Raman spectroscopy: application to clean-room-relevant biological contaminations.	Rosch P, Harz M, Schmitt M, Peschke KD, Ronneberger O, Burkhardt H, Motzkus HW, Lankers M, Hofer S, Thiele H, Popp J.	Appl Environ Microbiol	10.1128/aem.71.3.1626-1637.2005	2005
	A critical assessment of microbial-based antimicrobial sanitizing of inanimate surfaces in healthcare settings.	Fijan S, Kurti P, Rozman U, Sostar Turk S.	Front Microbiol	10.3389/fmicb.2024.1412269	2024
	Antibacterial effect of ethanolic Gnetum gnemon L. leaf extract on food-borne pathogens and its application as a natural preservative on raw quail eggs.	Trisha MR, Deavyndra Gunawan V, Wong JX, Pak Dek MS, Rukayadi Y.	Heliyon	10.1016/j.heliyon.2024.e35691	2024
	Investigating the in vitro antibacterial, antibiofilm, antioxidant, anticancer and antiviral activities of zinc oxide nanoparticles biofabricated from Cassia javanica.	Almuhayawi MS, Alruhaili MH, Soliman MKY, Tarabulsi MK, Ashy RA, Saddiq AA, Selim S, Alruwaili Y, Salem SS.	PLoS One	10.1371/journal.pone.0310927	2024
	Optimized Chemical Extraction Methods of Antimicrobial Peptides from Roots and Leaves of Extremophilic Plants: Anthyllis sericea and Astragalus armatus Collected from the Tunisian Desert.	Ben Brahim R, Ellouzi H, Fouzai K, Asses N, Neffati M, Sabatier JM, Bulet P, Regaya I.	Antibiotics (Basel)	10.3390/antibiotics11101302	2022
	Pharmacological Properties of Platycarpha glomerata Extracts-A Plant Used to Treat and Manage Elephantiasis.	Lamula SQN, Taliwe A, Buwa-Komoreng LV.	Int J Mol Sci	10.3390/ijms26020646	2025
	Controlling vegetative cells and spores growth of Bacillus spp. using ethanolic Ketapang (Terminalia catappa L.) leaf extract.	Madhavan K, Rukayadi Y, Abdul-Mutalib NA.	Heliyon	10.1016/j.heliyon.2023.e18749	2023
	Bioactive compounds, antibacterial and antioxidant activities of methanol extract of Tamarindus indica Linn.	Fagbemi KO, Aina DA, Adeoye-Isijola MO, Naidoo KK, Coopoosamy RM, Olajuyigbe OO.	Sci Rep	10.1038/s41598-022-13716-x	2022
	Biomedical Promise of Aspergillus Flavus-Biosynthesized Selenium Nanoparticles: A Green Synthesis Approach to Antiviral, Anticancer, Anti-Biofilm, and Antibacterial Applications.	Mohammed EJ, Abdelaziz AEM, Mekky AE, Mahmoud NN, Sharaf M, Al-Habibi MM, Khairy NM, Al-Askar AA, Youssef FS, Gaber MA, Saied E, AbdElgayed G, Metwally SA, Shoun AA.	Pharmaceuticals (Basel)	10.3390/ph17070915	2024
	Assessment of the phytochemical, antioxidant and antibacterial activities of Heteromorpha arborescens (Spreng.) Cham & Schltdl. leaf extracts.	Abifarin TO, Otunola GA, Afolayan AJ.	F1000Res	10.12688/f1000research.25197.1	2020
	Warmed-over flavour profiles, microbial changes, shelf-life and check-all-that-apply sensory analysis of cooked minced pork treated with varying levels of Moringa oleifera leaf and root powder.	N S L, A J A, E M I.	Heliyon	10.1016/j.heliyon.2022.e10616	2022
Metabolism	Elucidation of auxotrophic deficiencies of Bacillus pumilus DSM 18097 to develop a defined minimal medium.	Muller J, Beckers M, Mussmann N, Bongaerts J, Buchs J.	Microb Cell Fact	10.1186/s12934-018-0956-1	2018
Pathogenicity	In vitro antibacterial and time-kill evaluation of the Erythrina caffra Thunb. extract against bacteria associated with diarrhoea.	Olajuyigbe OO, Afolayan AJ.	ScientificWorldJournal	10.1100/2012/738314	2012
	Development and validation of microbial bioassay for quantification of Levofloxacin in pharmaceutical preparations.	Dafale NA, Semwal UP, Agarwal PK, Sharma P, Singh GN.	J Pharm Anal	10.1016/j.jpha.2014.07.007	2015
	Assessment of genetic diversity of Bacillus spp. isolated from eutrophic fish culture pond.	Sen R, Tripathy S, Padhi SK, Mohanty S, Maiti NK.	3 Biotech	10.1007/s13205-014-0234-9	2015
	Molecular Diagnostic for Prospecting Polyhydroxyalkanoate-Producing Bacteria.	Montenegro EMDS, Delabary GS, Silva MACD, Andreote FD, Lima AOS.	Bioengineering (Basel)	10.3390/bioengineering4020052	2017
	Differentiation of Bacillus pumilus and Bacillus safensis using MALDI-TOF-MS.	Branquinho R, Sousa C, Lopes J, Pintado ME, Peixe LV, Osorio H.	PLoS One	10.1371/journal.pone.0110127	2014
Pathogenicity	In vitro antibacterial and time-kill assessment of crude methanolic stem bark extract of Acacia mearnsii de wild against bacteria in shigellosis.	Olajuyigbe OO, Afolayan AJ.	Molecules	10.3390/molecules17022103	2012
Pathogenicity	Pharmacological assessment of the medicinal potential of Acacia mearnsii De Wild.: antimicrobial and toxicity activities.	Olajuyigbe OO, Afolayan AJ.	Int J Mol Sci	10.3390/ijms13044255	2012
Pathogenicity	Antimicrobial activity of the solvent fractions from Bulbine natalensis Tuber.	Yakubu MT, Mostafa M, Ashafa AO, Afolayan AJ.	Afr J Tradit Complement Altern Med	10.4314/ajtcam.v9i4.2	2012
Pathogenicity	Interactions of antibiotics and methanolic crude extracts of Afzelia Africana (Smith.) against drug resistance bacterial isolates.	Aiyegoro O, Adewusi A, Oyedemi S, Akinpelu D, Okoh A.	Int J Mol Sci	10.3390/ijms12074477	2011
	Metal complexes of diisopropylthiourea: synthesis, characterization and antibacterial studies.	Ajibade PA, Zulu NH.	Int J Mol Sci	10.3390/ijms12107186	2011
Pathogenicity	Chemical composition and antibacterial activity of the essential oils of Callistemon citrinus and Callistemon viminalis from South Africa.	Oyedeji OO, Lawal OA, Lawal OA, Shode FO, Oyedeji AO.	Molecules	10.3390/molecules14061990	2009
	Partial purification and characterization of an antimicrobial activity from the wood extract of mangrove plant Ceriops decandra.	Simlai A, Mukherjee K, Mandal A, Bhattacharya K, Samanta A, Roy A.	EXCLI J	10.17179/excli2015-741	2016
	Phytochemicals analysis and medicinal potentials of hydroalcoholic extract from Curtisia dentata (Burm.f) C.A. Sm Stem Bark.	Oyedemi SO, Oyedemi BO, Arowosegbe S, Afolayan AJ.	Int J Mol Sci	10.3390/ijms13056189	2012
	A Novel Cell Type Enables B. subtilis to Escape from Unsuccessful Sporulation in Minimal Medium.	Defeu Soufo HJ.	Front Microbiol	10.3389/fmicb.2016.01810	2016
	Antimicrobial activity of Bauhinia tomentosa and Bauhinia vahlii roots.	Dugasani S, Balijepalli MK, Tandra S, Pichika MR.	Pharmacogn Mag	10.4103/0973-1296.66937	2010
	Synthesis, reactions and antimicrobial activities of 8-ethoxycoumarin derivatives.	Mohamed HM, Abd El-Wahab AH, Ahmed KA, El-Agrody AM, Bedair AH, Eid FA, Khafagy MM.	Molecules	10.3390/molecules17010971	2012
	Synthesis, Characterization, and Antibacterial Studies of Pd(II) and Pt(II) Complexes of Some Diaminopyrimidine Derivatives.	Ajibade PA, Idemudia OG.	Bioinorg Chem Appl	10.1155/2013/549549	2013
Enzymology	Identification of Bacillus subtilis NRRL B-3275 as a strain of Bacillus pumilus.	Lovett PS, Young FE.	J Bacteriol	10.1128/jb.100.2.658-661.1969	1969
	Spontaneous auxotrophic and pigmented mutants occurring at high frequency in Bacillus pumilus NRRL B-3275.	Lovett PS.	J Bacteriol	10.1128/jb.112.2.977-985.1972	1972
	The second international reference preparation of lymecycline.	Lightbown JW, Thomas AH, Berryman IL.	Bull World Health Organ		1973
	Applications of alkyl orthoesters as valuable substrates in organic transformations, focusing on reaction media.	Khademi Z, Nikoofar K.	RSC Adv	10.1039/d0ra05276k	2020
	Corrected Version Distribution of Heterogeneous and Homologous Plasmids in Bacillus spp.	Yoshimura K, Yamamoto O, Seki T, Oshima Y.	Appl Environ Microbiol	10.1128/aem.46.6.1268-1275.1983	1983
	Distribution of heterogeneous and homologous plasmids in Bacillus spp.	Yoshimura K, Yamamoto O, Seki T, Oshima Y.	Appl Environ Microbiol	10.1128/aem.45.6.1733-1740.1983	1983
	Antibiotic Activity of Actinobacteria from the Digestive Tract of Millipede Nedyopus dawydoffiae (Diplopoda).	Glukhova AA, Karabanova AA, Yakushev AV, Semenyuk II, Boykova YV, Malkina ND, Efimenko TA, Ivankova TD, Terekhova LP, Efremenkova OV.	Antibiotics (Basel)	10.3390/antibiotics7040094	2018
Pathogenicity	Fluorescein Derivatives as Antibacterial Agents Acting via Membrane Depolarization.	Nazarov PA, Kirsanov RS, Denisov SS, Khailova LS, Karakozova MV, Lyamzaev KG, Korshunova GA, Lukyanov KA, Kotova EA, Antonenko YN.	Biomolecules	10.3390/biom10020309	2020
	Antimicrobial Activity of Microorganisms Isolated from Ant Nests of Lasius niger.	Efimenko TA, Glukhova AA, Demiankova MV, Boykova YV, Malkina ND, Sumarukova IG, Vasilieva BF, Rogozhin EA, Ivanov IA, Krassilnikov VA, Efremenkova OV.	Life (Basel)	10.3390/life10060091	2020
	Great horsetail (Equisetum telmateia Ehrh.): Active substances content and biological effects.	Radojevic ID, Stankovic MS, Stefanovic OD, Topuzovic MD, Comic LR, Ostojic AM.	EXCLI J		2012
	Immortelle (Xeranthemum annuum L.) as a natural source of biologically active substances.	Stankovic MS, Radojevic ID, Stefanovic OD, Topuzovic MD, Comic LR, Brankovic SR.	EXCLI J		2011
	An Investigation on in vitro and in vivo Antimicrobial Properties of the Antidepressant: Amitriptyline Hydrochloride.	Mandal A, Sinha C, Kumar Jena A, Ghosh S, Samanta A.	Braz J Microbiol	10.1590/s1517-83822010000300014	2010
	Platismatia glaucia and Pseudevernia furfuracea lichens as sources of antioxidant, antimicrobial and antibiofilm agents.	Mitrovic T, Stamenkovic S, Cvetkovic V, Radulovic N, Mladenovic M, Stankovic M, Topuzovic M, Radojevic I, Stefanovic O, Vasic S, Comic L.	EXCLI J		2014
Pathogenicity	Effects of a combination of amlodipine and imipenem on 42 clinical isolates of Acinetobacter baumannii obtained from a teaching hospital in Guangzhou, China.	Li Yj, Pan Cz, Zhao Zw, Zhao Zx, Chen Hl, Lu Wb.	BMC Infect Dis	10.1186/1471-2334-13-548	2013
	The second international standard for tetracycline.	Lightbown JW, Thomas AH, Berryman IL.	Bull World Health Organ		1973
	The international standard for rolitetracycline.	Lightbown JW, Bond JM, Mussett MV, Tackett LL.	Bull World Health Organ		1973
	The second International Standard for Oxytetracycline.	Lightbown JW, Bond JM, Grab B.	Bull World Health Organ		1967
Phylogeny	Direct and rapid identification and genogrouping of meningococci and porA amplification by LightCycler PCR.	Molling P, Jacobsson S, Backman A, Olcen P.	J Clin Microbiol	10.1128/jcm.40.12.4531-4535.2002	2002
Phylogeny	[Identification and Antagonistic Properties of the Soil Streptomycete Streptomyces sp. 100].	Biliavska LA, Efimenko TA, Efremenkova OV, Koziritska VY, Iutynska GA	Mikrobiol Z		2016
Metabolism	Antibiotic producing potentials of three freshwater actinomycetes isolated from the Eastern Cape Province of South Africa.	Sibanda T, Mabinya LV, Mazomba N, Akinpelu DA, Bernard K, Olaniran AO, Okoh AI	Int J Mol Sci	10.3390/ijms11072612	2010
Metabolism	Acetoin catabolism and acetylbutanediol formation by Bacillus pumilus in a chemically defined medium.	Xiao Z, Ma C, Xu P, Lu JR	PLoS One	10.1371/journal.pone.0005627	2009
Pathogenicity	Flow microcalorimetric assay of antibiotics--II. Neomycin sulphate and its combinations with polymyxin B sulphate and zinc bacitracin on interaction with Bacillus pumilus (NCTC 8241).	Joslin Kjeldsen N, Beezer AE, Miles RJ	J Pharm Biomed Anal	10.1016/0731-7085(89)80006-8	1989
Pathogenicity	[Comparative study of the quantitative determination of kanamycin sulfate in ophthalmic films with a collagen base by microbiological and chemical methods].	Ivanova LA, Abramova TA, Levkovich NM, Okhotnikova VF, Kartashov VS	Antibiotiki		1984

References

#151	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 361
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#20218	Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )
#36568	; Curators of the CIP;
#44628	Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 3273
#68368	Automatically annotated from API 20E .
#68371	Automatically annotated from API 50CH acid .
#68379	Automatically annotated from API Coryne .
#68382	Automatically annotated from API zym .
#122059	Collection of Institut Pasteur ; Curators of the CIP; CIP 76.18
#124043	Isabel Schober, Julia Koblitz: Data extracted from sequence databases, automatically matched based on designation and taxonomy .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Bacillus pumilus (DSM 361, ATCC 14884, CCM 2218)

Name and taxonomic classification