Name: Amnibacterium kyonggiense KSL51201-037
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 7508

Type strain:

Species: Amnibacterium kyonggiense

Strain Designation: KSL51201-037

Culture col. no.: DSM 24782, JCM 16463, KEMC 51201-037, KACC 15557, NBRC 109360

Strain history: S.-S. Lee KSL51201-037.

NCBI tax ID(s): 595671 (species)

SI-ID 401682

DSM 24782

Section

Amnibacterium kyonggiense KSL51201-037 is an aerobe, mesophilic, Gram-positive bacterium that was isolated from Anyang stream.

Gram-positive
rod-shaped
aerobe
mesophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Actinomycetota*
	Class	*Actinomycetes*
	Order	*Micrococcales*
	Family	*Microbacteriaceae*
	Genus	*Amnibacterium*
	Species	**Amnibacterium kyonggiense**
	Full Scientific Name (LPSN)	Amnibacterium kyonggiense Kim and Lee 2011

Information on morphological and physiological properties Morphology

[Ref.: #29690]	Gram stain	positive
[Ref.: #69480]	Gram stain	positive Confidence in %100

[Ref.: #29690]	Cell length	0.275 µm

[Ref.: #29690]	Cell width	0.175 µm

[Ref.: #29690]	Cell shape	rod-shaped

[Ref.: #29690]	Motility	no
[Ref.: #69480]	Motility	no Confidence in %93.286

[Ref.: #21380]	Colony color	Lemon yellow (1012)
[Ref.: #21380]	Incubation period	10-14 days
[Ref.: #21380]	Cultivation medium used	ISP 2

[Ref.: #21380]	Colony color	n.d.
[Ref.: #21380]	Incubation period	10-14 days
[Ref.: #21380]	Cultivation medium used	ISP 3

[Ref.: #21380]	Colony color	none
[Ref.: #21380]	Incubation period	10-14 days
[Ref.: #21380]	Cultivation medium used	ISP 4

[Ref.: #21380]	Colony color	none
[Ref.: #21380]	Incubation period	10-14 days
[Ref.: #21380]	Cultivation medium used	ISP 5

[Ref.: #21380]	Colony color	none
[Ref.: #21380]	Incubation period	10-14 days
[Ref.: #21380]	Cultivation medium used	ISP 7

[Ref.: #21380]	Colony color	none
[Ref.: #21380]	Incubation period	10-14 days
[Ref.: #21380]	Cultivation medium used	Suter with tyrosine

[Ref.: #21380]	Colony color	none
[Ref.: #21380]	Incubation period	10-14 days
[Ref.: #21380]	Cultivation medium used	Suter without tyrosine

[Ref.: #29690]

Pigment production

yes

[Ref.: #21380]	Multicellular complex forming ability	no
[Ref.: #21380]	Medium Name (multicellularity)	ISP 2

[Ref.: #21380]	Multicellular complex forming ability	no
[Ref.: #21380]	Medium Name (multicellularity)	ISP 3

[Ref.: #21380]	Multicellular complex forming ability	no
[Ref.: #21380]	Medium Name (multicellularity)	ISP 4

[Ref.: #21380]	Multicellular complex forming ability	no
[Ref.: #21380]	Medium Name (multicellularity)	ISP 5

[Ref.: #21380]	Multicellular complex forming ability	no
[Ref.: #21380]	Medium Name (multicellularity)	ISP 7

[Ref.: #21380]	Multicellular complex forming ability	no
[Ref.: #21380]	Medium Name (multicellularity)	Suter with tyrosine

[Ref.: #21380]	Multicellular complex forming ability	no
[Ref.: #21380]	Medium Name (multicellularity)	Suter without tyrosine

Information on culture and growth conditions Culture and growth conditions

[Ref.: #17770]

Culture medium

R2A MEDIUM (DSMZ Medium 830)

[Ref.: #17770]

Culture medium growth

[Ref.: #17770]

Culture medium link

Medium recipe at MediaDive

[Ref.: #17770]

Culture medium composition

expand / minimize

[Ref.: #21380]

Culture medium

ISP 2

[Ref.: #21380]

Culture medium growth

[Ref.: #21380]

Culture medium composition

expand / minimize

[Ref.: #21380]

Culture medium

ISP 3

[Ref.: #21380]

Culture medium growth

[Ref.: #21380]

Culture medium composition

expand / minimize

[Ref.: #21380]

Culture medium

ISP 4

[Ref.: #21380]

Culture medium growth

[Ref.: #21380]

Culture medium composition

expand / minimize

[Ref.: #21380]

Culture medium

ISP 5

[Ref.: #21380]

Culture medium growth

[Ref.: #21380]

Culture medium composition

expand / minimize

[Ref.: #21380]

Culture medium

ISP 7

[Ref.: #21380]

Culture medium growth

[Ref.: #21380]

Culture medium composition

expand / minimize

[Ref.: #21380]

Culture medium

Suter with tyrosine

[Ref.: #21380]

Culture medium growth

[Ref.: #21380]

Culture medium composition

expand / minimize

[Ref.: #21380]

Culture medium

Suter without tyrosine

[Ref.: #21380]

Culture medium growth

[Ref.: #21380]

Culture medium composition

expand / minimize

		Temperature
[Ref.: #17770]	growth	28 °C
[Ref.: #29690]	growth	10-37 °C
[Ref.: #29690]	optimum	30 °C
[Ref.: #67770]	growth	28 °C

[Ref.: #17770]	Temperature range	mesophilic
[Ref.: #29690]	Temperature range	mesophilic
[Ref.: #67770]	Temperature range	mesophilic

	pH	Kind of pH		pH
[Ref.: #29690]			optimum	6
[Ref.: #29690]			growth	5.0-8.0

Information on physiology and metabolism Physiology and metabolism

[Ref.: #29690]

Oxygen tolerance

aerobe

[Ref.: #69481]	Ability of spore formation	no Confidence in %99
[Ref.: #69480]	Ability of spore formation	no Confidence in %99.998

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #21380]		NaCl		growth	0	%
[Ref.: #29690]		NaCl		growth	0-2	%

[Ref.: #29690]	Observation	aggregates in clumps
[Ref.: #67770]	Observation	quinones: MK-11, MK-12

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #21380]	arabinose ChEBI	-
[Ref.: #21380]	cellulose ChEBI	-
[Ref.: #68379]	D-glucose ChEBI	-	fermentation	* from API Coryne
[Ref.: #68379]	D-mannitol ChEBI	-	fermentation	* from API Coryne
[Ref.: #68379]	D-ribose ChEBI	-	fermentation	* from API Coryne
[Ref.: #68379]	D-xylose ChEBI	-	fermentation	* from API Coryne
[Ref.: #29690]	esculin ChEBI	+	hydrolysis
[Ref.: #68379]	esculin ChEBI	+	hydrolysis	* from API Coryne
[Ref.: #21380]	fructose ChEBI	-
[Ref.: #68379]	gelatin ChEBI	-	hydrolysis	* from API Coryne
[Ref.: #21380]	glucose ChEBI	-
[Ref.: #68379]	glycogen ChEBI	-	fermentation	* from API Coryne
[Ref.: #68379]	lactose ChEBI	-	fermentation	* from API Coryne
[Ref.: #68379]	maltose ChEBI	-	fermentation	* from API Coryne
[Ref.: #21380]	mannose ChEBI	-
[Ref.: #21380]	myo-inositol ChEBI	-
[Ref.: #68379]	nitrate ChEBI	-	reduction	* from API Coryne
[Ref.: #21380]	raffinose ChEBI	-
[Ref.: #21380]	rhamnose ChEBI	-
[Ref.: #21380]	sucrose ChEBI	-
[Ref.: #68379]	sucrose ChEBI	-	fermentation	* from API Coryne
[Ref.: #68379]	urea ChEBI	-	hydrolysis	* from API Coryne
[Ref.: #21380]	xylose ChEBI	-
	Only first 10 entries are displayed. Click here to see all.

	Enzyme	Enzyme activity	EC number
[Ref.: #29690]	acid phosphatase	+	3.1.3.2
[Ref.: #68382]	acid phosphatase	+	3.1.3.2	* from API zym
[Ref.: #68379]	alkaline phosphatase	-	3.1.3.1	* from API Coryne
[Ref.: #68382]	alpha-chymotrypsin	-	3.4.21.1	* from API zym
[Ref.: #68382]	alpha-fucosidase	-	3.2.1.51	* from API zym
[Ref.: #29690]	alpha-galactosidase	+	3.2.1.22
[Ref.: #68382]	alpha-galactosidase	+	3.2.1.22	* from API zym
[Ref.: #68379]	alpha-glucosidase	-	3.2.1.20	* from API Coryne
[Ref.: #68382]	alpha-mannosidase	-	3.2.1.24	* from API zym
[Ref.: #68382]	beta-galactosidase	-	3.2.1.23	* from API zym, API Coryne
[Ref.: #68382]	beta-glucosidase	+	3.2.1.21	* from API zym, API Coryne
[Ref.: #68382]	beta-glucuronidase	-	3.2.1.31	* from API zym, API Coryne
[Ref.: #29690]	catalase	+	1.11.1.6
[Ref.: #68382]	esterase (C 4)	+		* from API zym
[Ref.: #68382]	esterase lipase (C 8)	+		* from API zym
[Ref.: #68379]	gelatinase	-		* from API Coryne
[Ref.: #68382]	leucine arylamidase	+	3.4.11.1	* from API zym
[Ref.: #68382]	lipase (C 14)	-		* from API zym
[Ref.: #68382]	N-acetyl-beta-glucosaminidase	+	3.2.1.52	* from API zym
[Ref.: #68379]	N-acetyl-beta-glucosaminidase	-	3.2.1.52	* from API Coryne
[Ref.: #68382]	naphthol-AS-BI-phosphohydrolase	+		* from API zym
[Ref.: #68379]	pyrazinamidase	-	3.5.1.B15	* from API Coryne
[Ref.: #68379]	pyrrolidonyl arylamidase	-	3.4.19.3	* from API Coryne
[Ref.: #68382]	trypsin	-	3.4.21.4	* from API zym
[Ref.: #68379]	urease	-	3.5.1.5	* from API Coryne
	Only first 10 entries are displayed. Click here to see all.

API® Coryne:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Reduction of nitrateNIT	PyrazinamidasePYZ	Pyrrolidonyl arylamidasePYRA	Alkaline phosphatasePAL	beta-Glucuronidasebeta GUR	beta-Galactosidasebeta GAL	alpha-Glucosidasealpha GLU	N-Acetyl-beta-Glucosidasebeta NAG	Esculin hydrolysis/beta-GlucosidaseESC	Urease/urea hydrolysisURE	Gelatinase/gelatin hydrolysisGEL	Control fermentationControl	Fermentation of D-glucoseGLU	Fermentation of D-riboseRIB	Fermentation of D-xyloseXYL	Fermentation of D-mannitolMAN	Fermentation of maltoseMAL	Fermentation of lactoseLAC	Fermentation of sucroseSAC	Fermentation of glycogenGLYG	CatalaseCAT
[Ref.: #21380]	509	-	-	-	-	-	-	-	-	+	-	-	not determinedn.d.	-	-	-	-	-	-	-	-	not determinedn.d.

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #21380]	1021	not determinedn.d.	+/-	+	+	-	+	+/-	+/-	-	-	+	+	+	-	-	+/-	+	+	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #17770]	Sample type/isolated from	Anyang stream
[Ref.: #17770]	Country	Republic of Korea
[Ref.: #17770]	Country ISO 3 Code	KOR
[Ref.: #17770]	Continent	Asia

[Ref.: #67770]	Sample type/isolated from	Anyang stream
[Ref.: #67770]	Country	Republic of Korea
[Ref.: #67770]	Country ISO 3 Code	KOR
[Ref.: #67770]	Continent	Asia
* marker position based on {}

Isolation sources categories

#Environmental

#Aquatic

#River (Creek)

What are isolation sources categories?

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #17770]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #17770]	Amnibacterium kyonggiense 16S ribosomal RNA gene, partial sequence	FJ527819	1459	ENA	595671 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #67770]	Amnibacterium kyonggiense DSM 24782	GCA_004364555	contig	GenBank	595671 tax ID
[Ref.: #66792]	Amnibacterium kyonggiense strain DSM 24782	595671.3	wgs	PATRIC	595671 tax ID	*
[Ref.: #66792]	Amnibacterium kyonggiense DSM 24782	2734482167	draft	JGI IMG/M	595671 tax ID	*

[Ref.: #17770]	GC-content	72.7 mol%	high performance liquid chromatography (HPLC)
[Ref.: #29690]	GC-content	72.7 mol%

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Amnibacterium kyonggiense strain DSM 24782 PATRIC: 595671.3
[Ref.: #69481]	spore-forming	no	99	no

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Amnibacterium kyonggiense strain DSM 24782 PATRIC: 595671.3
[Ref.: #69480]	gram-positive	yes	90.647	no
[Ref.: #69480]	anaerobic	no	99.429	yes
[Ref.: #69480]	halophile	no	94.048	no
[Ref.: #69480]	spore-forming	no	93.638	no
[Ref.: #69480]	glucose-util	yes	78.244	yes
[Ref.: #69480]	thermophile	no	99.489	no
[Ref.: #69480]	flagellated	no	95.477	yes
[Ref.: #69480]	aerobic	yes	89.811	yes
[Ref.: #69480]	motile	no	63.609	yes
[Ref.: #69480]	glucose-ferment	no	89.039	yes

Availability in culture collections External links

[Ref.: #17770]

Culture collection no.

DSM 24782, JCM 16463, KEMC 51201-037, KACC 15557, NBRC 109360

[Ref.: #76954]

SI-ID 401682

Literature:

Only first 10 entries are displayed. Click here to see all.

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Naasia lichenicola sp. nov., an actinobacterium isolated from lichen.	Jiang LQ, Zhang K, Li GD, Wang XY, Shi SB, Li QY, An DF, Lang L, Wang LS, Jiang CL, Jiang Y	Int J Syst Evol Microbiol	10.1099/ijsem.0.003865	2020	*
Phylogeny	Amnibacterium setariae sp. nov., an endophytic actinobacterium isolated from dried foxtail.	Kim I, Chhetri G, Kim J, Seo T	Antonie Van Leeuwenhoek	10.1007/s10482-019-01302-7	2019	*
Phylogeny	Amnibacterium flavum sp. nov., a novel endophytic actinobacterium isolated from bark of Nerium indicum Mill.	Tuo L, Yan XR, Li FN, Yang C, An MB, Sun CH	Int J Syst Evol Microbiol	10.1099/ijsem.0.003156	2018	*
Phylogeny	Amnibacterium endophyticum sp. nov., an endophytic actinobacterium isolated from Aegiceras corniculatum.	Li FN, Tuo L, Lee SM, Jin T, Liao S, Li W, Yan X, Sun CH	Int J Syst Evol Microbiol	10.1099/ijsem.0.002676	2018	*
Phylogeny	Amnibacterium soli sp. nov., an actinobacterium isolated from grass soil.	Jin L, Lee HG, Kim HS, Ahn CY, Oh HM	Int J Syst Evol Microbiol	10.1099/ijs.0.052506-0	2013	*
Phylogeny	Naasia aerilata gen. nov., sp. nov., a member of the family Microbacteriaceae isolated from air.	Weon HY, Kim SJ, Jang YH, Hamada M, Tamura T, Ahn JH, Suzuki KI, Kwon SW	Int J Syst Evol Microbiol	10.1099/ijs.0.046599-0	2012	*
Phylogeny	Amnibacterium kyonggiense gen. nov., sp. nov., a new member of the family Microbacteriaceae.	Kim SJ, Lee SS	Int J Syst Evol Microbiol	10.1099/ijs.0.018788-0	2010	*

References

#17770

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 24782

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#21380

Wink, J.: Compendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig .

#29690

Barberan A, Caceres Velazquez H, Jones S, Fierer N.: Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information. mSphere 2: 2017 ( DOI 10.1128/mSphere.00237-17 , PubMed 28776041 ) - originally annotated from #26075 (see below)

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#68379

Automatically annotated from API Coryne .

#68382

Automatically annotated from API zym .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#76954

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID401682.1 )

#26075

IJSEM 155 2011 ( DOI 10.1099/ijs.0.018788-0 , PubMed 20173001 )

* These data were automatically processed and therefore are not curated

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Amnibacterium kyonggiense strain DSM 24782

Amnibacterium kyonggiense strain DSM 24782

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