Microbacterium liquefaciens (DSM 20638, ATCC 43647, NCIB 11509)

Name: Microbacterium liquefaciens (DSM 20638, ATCC 43647, NCIB 11509)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 7380

Type strain

Strain Designation Mbm 15

Culture col. no. DSM 20638 ATCC 43647 NCIB 11509 NRRL B-24237 CCUG 33091 16 more

NCBI tax ID(s) 33918

Special Collections DSMZ CCUG JCM KCTC CIP Wink compendium JMRC

History DSMZ <- NCIB <- K. Robinson, Mbm 15 (Microbacterium liquefaciens) JCM <-- DSM 20638 <-- NCIB 11509 <-- K. Robinson Mbm 15. CIP <- 1986, DSM <- NCIB <- K. Robinson: strain Mbm15, Microbacterium liquefaciens

Links

Microbacterium liquefaciens Mbm 15 is an aerobe, Gram-positive, rod-shaped bacterium that was isolated from milk.

Gram-positive rod-shaped aerobe genome sequence 16S sequence Bacteria

Name and taxonomic classification

SI-ID 45479

LMG 16342,ATCC 43647,CCRC 14859,CCUG 33091,CIP 102402,DSM 20638,IFO 15037,JCM 3879,NCIMB 11509,VKM Ac-1018,NCIB 11509,KCTC 9227,NBRC 15037,BCRC 14859,HAMBI 1895,NRRL B-24237,IAM 15185,CGMCC 1.1934

@ref 20215

Last LPSN update 2026-05-29 08:26:38

Domain Bacteria

Phylum Actinomycetota

Class Actinomycetes

Order Micrococcales

Family Microbacteriaceae

Genus Microbacterium

Species Microbacterium liquefaciens

Full scientific name Microbacterium liquefaciens (Collins et al. 1983 ex Orla-Jensen 1919) Takeuchi and Hatano 1998

Synonyms (1)

Aureobacterium liquefaciens

BacDive ID	Other strains from **Microbacterium liquefaciens** (5)	Type strain
7379	M. liquefaciens Mbm 18A, DSM 20637, NCIB 11510, JCM 3878, ...
143979	M. liquefaciens CCUG 22214
146255	M. liquefaciens CCUG 30711
150666	M. liquefaciens CCUG 41919
150842	M. liquefaciens CCUG 42547

Morphology

Cell morphology

@ref	Gram stain	Cell shape	Motility	Confidence
120624	positive	rod-shaped
125438	positive			91.428

Colony morphology

@ref	Colony color	Incubation period	Medium used	Hemolysis ability
18766	Golden yellow	10-14 days	ISP 2
18766	Sulfur white	10-14 days	ISP 3
18766	Light ivory	10-14 days	ISP 4
18766		10-14 days	ISP 5
18766	Zinc yellow	10-14 days	ISP 6
18766		10-14 days	ISP 7
120624				0

Multicellular morphology

@ref	Forms multicellular complex	Medium name
18766		ISP 2
18766		ISP 3
18766		ISP 4
18766		ISP 5
18766		ISP 6
18766		ISP 7

Culture and growth conditions

Culture medium

@ref	Name	Medium link	Composition
8984	CORYNEBACTERIUM AGAR (DSMZ Medium 53)	Medium recipe at MediaDive	Name: CORYNEBACTERIUM AGAR (DSMZ Medium 53) Composition: Agar 15.0 g/l Casein peptone 10.0 g/l NaCl 5.0 g/l Glucose 5.0 g/l Yeast extract 5.0 g/l Distilled water
18766	ISP 2		Name: ISP 2 / Yeast Malt Agar (5265); 5265 Composition Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l Agar 15.0 g/l Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.0 Usage: Maintenance and Taxonomy Organisms: All Actinomycetes
18766	ISP 3		Name: ISP 3; 5315 Composition Dog oat flakes 20.0 g/l Trace element solution (5314) 2.5 ml/l Agar 18.0 g/l Preparation: Oat flakes are cooked for 20 minutes, trace element solution and agar are added (in the case of non rolled oat flakes the suspension has to bee filtrated). Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.8 Usage: Maintenance and taxonomy (e.g. SEM As liquid medium for metabolite production) Organisms: All Actinomycetes Trace element solution 5314 Name: Trace element solution 5314; 5314 Composition CaCl2 x H2O 3.0 g/l Fe-III-citrate 1.0 g/l MnSO4 0.2 g/l ZnCl2 0.1 g/l CuSO4 x 5 H2O 0.025 g/l Sodium tetra borate 0.2 g/l CoCl2 x 6 H2O 0.004 g/l Sodium molybdate 0.01 g/l Preparation: Use double destillated water. Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Trace element solution for different media Organisms:
18766	ISP 4		Name: ISP 4; DSM 547 Solution I: Difco soluble starch, 10.0 g. Make a paste of the starch with a small amount of cold distilled water and bring to a volume of 500 ml. Solution II: CaCO3 2.0 g K2HPO4 (anhydrous) 1.0 g MgSO4 x 7 H2O 1.0 g NaCl 1.0 g (NH4)2SO4 2.0 g Distilled water 500.0 ml Trace salt solution (see below) 1.0 ml The pH should be between 7.0 and 7.4. Do not adjust if it is within this range. Mix solutions I and II together. Add 20.0 g agar. Liquify agar by steaming at 100°C for 10 to 20 min. Trace element solution: FeSO4 x 7 H2O 0.1 g MnCl2 x 4 H2O 0.1 g ZnSO4 x 7 H2O 0.1 g Distilled water 100.0 ml
18766	ISP 5		Name: ISP 5 (5323) Composition L-Asparagine 1.0 g/l Glycerol 10.0 g/l K2HPO4 1.0 g/l Salt solution (see preparation) 1.0 ml/l Agar 20.0 g/l Preparation: Salt solution 1.0 g FeSO4 x 7 H2O 1.0 g MnCl2 x 4 H2O 1.0 g ZNSO4 x 7 H2O in 100 ml water Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.2 Usage: Maintenance and taxonomy Organisms: All Actinomycetes
18766	ISP 6		Name: ISP 6 (5318) Composition Peptone 15.0 g/l Proteose peptose 5.0 g/l Ferric ammonium citrate 0.5 g/l Sodium glycerophosphate 1.0 g/l Sodium thiosulfate 0.08 g/l Yeast extract 1.0 g/l Agar 15.0 g/l Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Production of melanoid pigments Organisms: All Actinomycetes
18766	ISP 7		Name: ISP 7 (5322) Composition Glycerol 15.0 g/l L-Tyrosine 0.5 g/l L-Asparagine 1.0 g/l K2HPO4 0.5 g/l NaCl 0.5 g/l FeSO4 x 7 H2O 0.01 g/l Trace element solution 5343 1.0 ml/l Agar 20.0 Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.3 Usage: Production of melanoid pigments Organisms: All Actinomycetes
40416	MEDIUM 3 - Columbia agar		Columbia agar (39.000 g);distilled water (1000.000 ml)
120624	CIP Medium 3	Medium recipe at CIP
120624	CIP Medium 72	Medium recipe at CIP

Culture temp

@ref	Growth	Type	Temperature (°C)
8984	positive	growth	30
18766	positive	optimum	28
40416	positive	growth	30
51372	positive	growth	37
67770	positive	growth	30
120624	positive	growth	10-37
120624	negative	growth	41
120624	negative	growth	45

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance	Confidence
51372	aerobe
120624	obligate aerobe
125439	obligate aerobe	97.655

Spore formation

@ref	Spore formation	Confidence
125439		95.878

Halophily

@ref	Salt	Growth	Tested relation	Concentration
18766	NaCl	positive	maximum	5 %
120624	NaCl	positive	growth	0-4 %
120624	NaCl		growth	6 %
120624	NaCl		growth	8 %
120624	NaCl		growth	10 %

Murein

@ref	Murein short key	Type
8984	B06	B2ß {Gly} [L-Hsr] D-Glu(Hyg)-Gly-D-Orn

Observation

67770

Observationquinones: MK-11, MK-12

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
18766	22599 ChEBI	arabinose	-
68368	29016 ChEBI	arginine	-	hydrolysis	from API 20E
18766	62968 ChEBI	cellulose	-
120624	16947 ChEBI	citrate	-	carbon source
68368	16947 ChEBI	citrate	-	assimilation	from API 20E
68379	17634 ChEBI	D-glucose	+	fermentation	from API Coryne
68379	16899 ChEBI	D-mannitol	+	fermentation	from API Coryne
68379	16988 ChEBI	D-ribose	+	fermentation	from API Coryne
68379	65327 ChEBI	D-xylose	-	fermentation	from API Coryne
68379	4853 ChEBI	esculin	-	hydrolysis	from API Coryne
120624	4853 ChEBI	esculin	+	hydrolysis
18766	28757 ChEBI	fructose	+
68379	5291 ChEBI	gelatin	-	hydrolysis	from API Coryne
68368	5291 ChEBI	gelatin	-	hydrolysis	from API 20E
18766	17234 ChEBI	glucose	-
68379	28087 ChEBI	glycogen	-	fermentation	from API Coryne
120624	606565 ChEBI	hippurate	+	hydrolysis
68379	17716 ChEBI	lactose	-	fermentation	from API Coryne
68368	25094 ChEBI	lysine	-	degradation	from API 20E
68379	17306 ChEBI	maltose	+	fermentation	from API Coryne
18766	29864 ChEBI	mannitol	-
18766	17268 ChEBI	myo-inositol	-
68379	17632 ChEBI	nitrate	+	reduction	from API Coryne
120624	17632 ChEBI	nitrate	-	reduction
120624	17632 ChEBI	nitrate	-	respiration
120624	16301 ChEBI	nitrite	-	reduction
68368	18257 ChEBI	ornithine	-	degradation	from API 20E
18766	16634 ChEBI	raffinose	-
18766	26546 ChEBI	rhamnose	-
18766	17992 ChEBI	sucrose	-
68379	17992 ChEBI	sucrose	+	fermentation	from API Coryne
68368	27897 ChEBI	tryptophan	-	energy source	from API 20E
68379	16199 ChEBI	urea	+	hydrolysis	from API Coryne
68368	16199 ChEBI	urea	-	hydrolysis	from API 20E
18766	18222 ChEBI	xylose	-

Antibiotic resistance

@ref	Metabolite	Is sensitive	Is resistant
120624	0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)

Metabolite production

@ref	Chebi-ID	Metabolite
68368	15688 ChEBI	acetoin	from API 20E
68368	16136 ChEBI	hydrogen sulfide	from API 20E
120624	35581 ChEBI	indole
68368	35581 ChEBI	indole	from API 20E

Metabolite tests

@ref	Chebi-ID	Metabolite	Voges-proskauer-test	Methylred-test	Indole test
120624	15688 ChEBI	acetoin	-
68368	15688 ChEBI	acetoin	-			from API 20E
120624	17234 ChEBI	glucose		-
68368	35581 ChEBI	indole			-	from API 20E

Enzymes

@ref	Value	Activity	Ec
120624	alcohol dehydrogenase	-	1.1.1.1
68382	alkaline phosphatase	+	3.1.3.1	from API zym
68379	alkaline phosphatase	-	3.1.3.1	from API Coryne
68382	alpha-chymotrypsin	-	3.4.21.1	from API zym
68382	alpha-fucosidase	-	3.2.1.51	from API zym
68382	alpha-galactosidase	+	3.2.1.22	from API zym
68379	alpha-glucosidase	+	3.2.1.20	from API Coryne
68382	alpha-glucosidase	+	3.2.1.20	from API zym
68382	alpha-mannosidase	+	3.2.1.24	from API zym
120624	amylase	-
68368	arginine dihydrolase	-	3.5.3.6	from API 20E
68382	beta-galactosidase	+	3.2.1.23	from API zym
120624	beta-galactosidase	+	3.2.1.23
68379	beta-galactosidase	+	3.2.1.23	from API Coryne
68368	beta-galactosidase	+	3.2.1.23	from API 20E
68382	beta-glucosidase	+	3.2.1.21	from API zym
68379	beta-glucosidase	-	3.2.1.21	from API Coryne
68382	beta-glucuronidase	-	3.2.1.31	from API zym
68379	beta-glucuronidase	+	3.2.1.31	from API Coryne
120624	caseinase	+	3.4.21.50
120624	catalase	+	1.11.1.6
68382	cystine arylamidase	+	3.4.11.3	from API zym
120624	DNase	+
120624	gamma-glutamyltransferase	-	2.3.2.2
120624	gelatinase	+
68379	gelatinase	-		from API Coryne
68368	gelatinase	-		from API 20E
120624	lecithinase	-
68382	leucine arylamidase	+	3.4.11.1	from API zym
120624	lipase	-
68382	lipase (C 14)	-		from API zym
120624	lysine decarboxylase	-	4.1.1.18
68368	lysine decarboxylase	-	4.1.1.18	from API 20E
68382	N-acetyl-beta-glucosaminidase	-	3.2.1.52	from API zym
68379	N-acetyl-beta-glucosaminidase	-	3.2.1.52	from API Coryne
120624	ornithine decarboxylase	-	4.1.1.17
68368	ornithine decarboxylase	-	4.1.1.17	from API 20E
120624	oxidase	-
120624	phenylalanine ammonia-lyase	-	4.3.1.24
120624	protease	+
68379	pyrazinamidase	+	3.5.1.B15	from API Coryne
68379	pyrrolidonyl arylamidase	+	3.4.19.3	from API Coryne
68382	trypsin	-	3.4.21.4	from API zym
120624	tryptophan deaminase	-
68368	tryptophan deaminase	-	4.1.99.1	from API 20E
120624	tween esterase	-
120624	urease	-	3.5.1.5
68379	urease	+	3.5.1.5	from API Coryne
68368	urease	-	3.5.1.5	from API 20E
68382	valine arylamidase	+		from API zym

API 20E

@ref	ONPG	ADH (Arg)	LDC (Lys)	ODC	CIT	H2S productionH2S	URE	TDA (Trp)	IND	Acetoin production (Voges Proskauer test)VP	GEL	GLU	MAN	INO	Sor	RHA	SAC	MEL	AMY	ARA	OX	Nitrite productionNO2	Reduction to N2N2	MotilityMOB	Growth on MacConkey mediumMAC	OF-O	OF-F
18766	+	-	-	-	-	-	-	-	-	-	-	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.

API coryne

@ref	Reduction of nitrateNIT	PYZ	PYRA	PAL	beta GUR	beta GAL	alpha GLU	beta NAG	ESC	URE	GEL	Control fermentationControl	GLU	RIB	XYL	MAN	MAL	LAC	SAC	GLYG	CAT
18766	+	+	+	-	+	+	+	-	-	+	-	not determinedn.d.	+	+	-	+	+	-	+	-	not determinedn.d.

API zym

@ref	Control	Alkaline phosphatase	Esterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	Lipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	Trypsin	alpha- Chymotrypsin	Acid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	alpha- Galactosidase	beta- Galactosidase	beta- Glucuronidase	alpha- Glucosidase	beta- Glucosidase	N-acetyl-beta- glucosaminidase	alpha- Mannosidase	alpha- Fucosidase
18766	not determinedn.d.	+	+	+	-	+	+	+	-	-	-	-	+	+	-	+	+	-	+	-
120624	-	+	-	-	-	+	+	+	-	-	+	+	+	+	-	+	+	-	+	-

API biotype100

@ref	ControlQ	D-glucose as carbon sourceGLU	D-fructose as carbon sourceFRU	D-galactose as carbon sourceGAL	D-trehalose as carbon sourceTRE	D-mannose as carbon sourceMNE	L-sorbose as carbon sourceSBE	D-melibiose as carbon sourceMEL	sucrose as carbon sourceSAC	D-raffinose as carbon sourceRAF	maltotriose as carbon sourceMTE	maltose as carbon sourceMAL	lactose as carbon sourceLAC	lactulose as carbon sourceLTE	1-O-methyl-beta-galactopyranoside as carbon sourceMbGa	1-O-methyl-alpha-galactopyranoside as carbon sourceMaGa	D-cellobiose as carbon sourceCEL	gentiobiose as carbon sourceGEN	1-O-methyl-beta-D-glucopyranoside as carbon sourceMbGu	esculin as carbon sourceESC	D-ribose as carbon sourceRIB	L-arabinose as carbon sourceARA	D-xylose as carbon sourceXYL	palatinose as carbon sourcePLE	L-rhamnose as carbon sourceRHA	L-fucose as carbon sourceFUC	D-melezitose as carbon sourceMLZ	D-arabitol as carbon sourceDARL	L-arabitol as carbon sourceLARL	xylitol as carbon sourceXLT	dulcitol as carbon sourceDUL	D-tagatose as carbon sourceTAG	glycerol as carbon sourceGLY	myo-inositol as carbon sourceINO	D-mannitol as carbon sourceMAN	maltitol as carbon sourceMTL	D-turanose as carbon sourceTUR	D-sorbitol as carbon sourceSOR	adonitol as carbon sourceADO	hydroxyquinoline-beta-glucuronide as carbon sourceHBG	D-lyxose as carbon sourceLYX	i-erythritol as carbon sourceERY	1-O-methyl-alpha-D-glucoside as carbon sourceMDG	3-O-methyl-D-glucose as carbon source3MDG	D-saccharate as carbon sourceSAT	mucate as carbon sourceMUC	L-tartrate as carbon sourceLTAT	D-tartrate as carbon sourceDTAT	meso-tartrate as carbon sourceMTAT	D-malate as carbon sourceDMLT	L-malate as carbon sourceLMLT	cis-aconitate as carbon sourceCATE	trans-aconitate as carbon sourceTATE	tricarballylate as carbon sourceTTE	citrate as carbon sourceCIT	D-glucuronate as carbon sourceGRT	D-galacturonate as carbon sourceGAT	2-ketogluconate as carbon source2KG	5-ketogluconate as carbon source5KG	tryptophan as carbon sourceTRY	N-acetyl-D-glucosamine as carbon sourceNAG	D-gluconate as carbon sourceGNT	phenylacetate as carbon sourcePAC	protocatechuate as carbon sourcePAT	4-hydroxybenzoate as carbon sourcepOBE	quinate as carbon sourceQAT	gentisate as carbon sourceGTE	3-hydroxybenzoate as carbon sourcemOBE	benzoate as carbon sourceBAT	3-phenylpropionate as carbon sourcePPAT	m-coumarate as carbon sourceCMT	trigonelline as carbon sourceTGE	betaine as carbon sourceBET	putrescine as carbon sourcePCE	4-aminobutyrate as carbon sourceABT	histamine as carbon sourceHIN	DL-lactate as carbon sourceLAT	caprate as carbon sourceCAP	caprylate as carbon sourceCYT	L-histidine as carbon sourceHIS	succinate as carbon sourceSUC	fumarate as carbon sourceFUM	glutarate as carbon sourceGRE	DL-glycerate as carbon sourceGYT	5-aminovalerate as carbon sourceAVT	ethanolamine as carbon sourceETN	tryptamine as carbon sourceTTN	D-glucosamine as carbon sourceGLN	itaconate as carbon sourceITA	3-hydroxybutyrate as carbon source3OBU	L-aspartate as carbon sourceAPT	L-glutamate as carbon sourceGTT	L-proline as carbon sourcePRO	D-alanine as carbon sourceDALA	L-alanine as carbon sourceLALA	L-serine as carbon sourceSER	malonate as carbon sourceMNT	propionate as carbon sourcePROP	L-tyrosine as carbon sourceTYR	2-ketoglutarate as carbon source2KT
120624	not determinedn.d.	+	+	+	-	+	-	-	-	-	+	+	+	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	+	-	-	-	-	-	-	-	+	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	+	-	-	-	-	-	-	-	-	-	+	-	-	-	-	-	+	-	-	-	-	-	-	-	-	-	-	-	-	-	+	+	-	-	-	-	-	-	-	-

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Host Body Product	#Fluids	#Milk
#Engineered	#Food production	#Dairy product

Isolation

@ref	Sample type
8984	milk
51372	Milk
67770	Milk
120624	Food, Milk

Taxonmaps

69479

Global distribution of 16S sequence X77444 (>99% sequence identity) for Microbacterium from Microbeatlas

Aquatic Samples	9581
Soil Samples	10113
Animal Samples	16954
Plant Samples	5605
Total Samples	42253

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
8984	1	Risk group (German classification) According to TRBA 466
18766	1	Risk group (German classification) According to TRBA 466
120624	1	Risk group (French classification) According to TRBA 466

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
66792	ASM1464875v1 assembly for Microbacterium maritypicum JCM 3879	scaffold	GCA_014648755	33918.5	8112172344	33918	63.5
67770	ASM653976v1 assembly for Microbacterium maritypicum NBRC 15037	contig	GCA_006539765	33918.3	8112099441	33918	62.89

16S sequences

@ref	Description	Accession	Length	NCBI tax ID
8984	A.liquefaciens (DSM 20638) 16S rRNA gene	X77444	1474	33918
124043	Microbacterium liquefaciens strain DSM 20638 16S ribosomal RNA gene, partial sequence.	PQ248414	601	33918
124043	Microbacterium liquefaciens strain JCM 3879 16S ribosomal RNA gene, partial sequence.	MT760470	1374	33918

GC content

@ref	GC-content (mol%)	Method
8984	68.6
67770	72.5	thermal denaturation, midpoint method (Tm)
67770	68.6	thermal denaturation, midpoint method (Tm)

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: ASM1464875v1 assembly for Microbacterium maritypicum JCM 3879
BV-BRC: 33918.5

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	oxygen_tolerance	BacteriaNetⓘ	obligate aerobe	97.66	no
125439	gram_stain	BacteriaNetⓘ	positive	82.62	no
125439	motility	BacteriaNetⓘ	no	67.86	no
125439	spore_formation	BacteriaNetⓘ	no	95.88	no

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Microbacterium liquefaciens JCM 3879
NCBI: GCA_014648755.1

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	yes	91.43	no
125438	anaerobic	anaerobicⓘ	no	98.60	no
125438	aerobic	aerobicⓘ	yes	85.11	yes
125438	spore-forming	spore-formingⓘ	no	79.12	no
125438	thermophilic	thermophileⓘ	no	96.47	yes
125438	flagellated	motile2+ⓘ	no	85.50	no

Literature

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Identification of a bacterial strain isolated from the liver of a laboratory mouse as Microbacterium paraoxydans and emended description of the species Microbacterium paraoxydans Laffineur et al 2003.	Buczolits S, Schumann P, Valens M, Rossello-Mora R, Busse HJ.	Indian J Microbiol	10.1007/s12088-008-0035-0	2008
	The evolution of reduced facilitation in a four-species bacterial community.	Piccardi P, Ulrich E, Garcia-Garcera M, Martino RD, Testa SEA, Mitri S.	Evol Lett	10.1093/evlett/qrae036	2024
	Complete Genome Sequences of Microbacterium liquefaciens Phages Mercedes, Leafus, Nebulous, and Ixel.	Frost VJ, Westover KM.	Microbiol Resour Announc	10.1128/mra.00068-21	2021
	Microbial and Sensory Quality Changes in Broiler Chicken Breast Meat During Refrigerated Storage.	Augustynska-Prejsnar A, Kacaniova M, Hanus P, Sokolowicz Z, Slowinski M.	Foods	10.3390/foods13244063	2024
	czcD gene from Bacillus megaterium and Microbacterium liquefaciens as a potential nickel-vanadium soil pollution biomarker.	Fierros-Romero G, Gomez-Ramirez M, Sharma A, Pless RC, Rojas-Avelizapa NG.	J Basic Microbiol	10.1002/jobm.201900323	2020
	Whole cell affinity for 4-amino-5-hydroxymethyl-2-methylpyrimidine (HMP) in the marine bacterium Candidatus Pelagibacter st. HTCC7211 explains marine dissolved HMP concentrations.	Brennan E, Noell S, Davis EW, Giovannoni SJ, Suffridge CP.	Environ Microbiol Rep	10.1111/1758-2229.70023	2024
Genetics	Four new Microbacterium species isolated from seaweeds and reclassification of five Microbacterium species with a proposal of Paramicrobacterium gen. nov. under a genome-based framework of the genus Microbacterium.	Lee SD, Yang HL, Kim IS.	Front Microbiol	10.3389/fmicb.2023.1299950	2023
Metabolism	Expression Changes in Metal-Resistance Genes in Microbacterium liquefaciens Under Nickel and Vanadium Exposure.	Fierros-Romero G, Wrosek-Cabrera JA, Gomez-Ramirez M, Pless RC, Rivas-Castillo AM, Rojas-Avelizapa NG.	Curr Microbiol	10.1007/s00284-017-1252-8	2017
	Artificial selection improves pollutant degradation by bacterial communities.	Arias-Sanchez FI, Vessman B, Haym A, Alberti G, Mitri S.	Nat Commun	10.1038/s41467-024-52190-z	2024
Transcriptome	Insights into the Molecular Mechanisms of Purine Compounds Synergistically Inducing Larval Settlement in Mytilopsis sallei Using Multi-Group Comparative Transcriptomic Analysis.	He J, Hao H, Pan H, Yao S, Zhao Y, Guo S, Huang J, Feng D.	Biology (Basel)	10.3390/biology13121067	2024
Metabolism	Identification of Bacillus megaterium and Microbacterium liquefaciens genes involved in metal resistance and metal removal.	Fierros-Romero G, Gomez-Ramirez M, Arenas-Isaac GE, Pless RC, Rojas-Avelizapa NG.	Can J Microbiol	10.1139/cjm-2015-0507	2016
	Repeated biocide treatments cause changes to the microbiome of a food industry floor drain biofilm model.	Kragh ML, Scheel NH, Leekitcharoenphon P, Truelstrup Hansen L.	Front Microbiol	10.3389/fmicb.2025.1542193	2025
Metabolism	Microbacterium oxydans and Microbacterium liquefaciens: a biological alternative for the treatment of Ni-V-containing wastes.	Gomez-Ramirez M, Montero-Alvarez LA, Tobon-Aviles A, Fierros-Romero G, Rojas-Avelizapa NG.	J Environ Sci Health A Tox Hazard Subst Environ Eng	10.1080/10934529.2015.994953	2015
	Substrate identification of putative NCS1 and NCS2 nucleobase transporters in Pseudomonas aeruginosa.	Kennelly C, Prindle A.	mBio	10.1128/mbio.02434-24	2024
	Microbiological Quality and Safety of Fresh Turkey Meat at Retail Level, Including the Presence of ESBL-Producing Enterobacteriaceae and Methicillin-Resistant S. aureus.	Martinez-Laorden A, Arraiz-Fernandez C, Gonzalez-Fandos E.	Foods	10.3390/foods12061274	2023
	Alternating access of a bacterial homolog of neurotransmitter: sodium symporters determined from AlphaFold2 ensembles and DEER spectroscopy.	Schwartz AC, Stein RA, Gil-Iturbe E, Quick M, Mchaourab HS.	Proc Natl Acad Sci U S A	10.1073/pnas.2406063121	2024
Phylogeny	Isolation of some pathogenic bacteria from the great spruce bark beetle, Dendroctonus micans and its specific predator, Rhizophagus grandis.	Yaman M, Erturk O, Aslan I.	Folia Microbiol (Praha)	10.1007/s12223-010-0006-9	2010
	Substrate Recognition Properties from an Intermediate Structural State of the UreA Transporter.	Sanguinetti M, Silva Santos LH, Dourron J, Alamon C, Idiarte J, Amillis S, Pantano S, Ramon A.	Int J Mol Sci	10.3390/ijms232416039	2022
Metabolism	Purification and properties of a new type of protease produced by Microbacterium liquefaciens.	Kanayama Y, Sakai Y.	Biosci Biotechnol Biochem	10.1271/bbb.69.916	2005
	Design of Topical Moxifloxacin Mucoadhesive Nanoemulsion for the Management of Ocular Bacterial Infections.	Youssef AAA, Thakkar R, Senapati S, Joshi PH, Dudhipala N, Majumdar S.	Pharmaceutics	10.3390/pharmaceutics14061246	2022
Metabolism	A novel recombinant system for functional expression of myonecrotic snake phospholipase A(2) in Escherichia coli using a new fusion affinity tag.	Seto M, Ogawa T, Kodama K, Muramoto K, Kanayama Y, Sakai Y, Chijiwa T, Ohno M.	Protein Expr Purif	10.1016/j.pep.2007.11.013	2008
Metabolism	Topological Dissection of the Membrane Transport Protein Mhp1 Derived from Cysteine Accessibility and Mass Spectrometry.	Calabrese AN, Jackson SM, Jones LN, Beckstein O, Heinkel F, Gsponer J, Sharples D, Sans M, Kokkinidou M, Pearson AR, Radford SE, Ashcroft AE, Henderson PJF.	Anal Chem	10.1021/acs.analchem.7b01310	2017
Metabolism	Dormant spores sense amino acids through the B subunits of their germination receptors.	Artzi L, Alon A, Brock KP, Green AG, Tam A, Ramirez-Guadiana FH, Marks D, Kruse A, Rudner DZ.	Nat Commun	10.1038/s41467-021-27235-2	2021
Metabolism	Crystallization of the hydantoin transporter Mhp1 from Microbacterium liquefaciens.	Shimamura T, Yajima S, Suzuki S, Rutherford NG, O'Reilly J, Henderson PJ, Iwata S.	Acta Crystallogr Sect F Struct Biol Cryst Commun	10.1107/s1744309108036920	2008
	Revealing the Tick Microbiome: Insights into Midgut and Salivary Gland Microbiota of Female Ixodes ricinus Ticks.	Wiesinger A, Wenderlein J, Ulrich S, Hiereth S, Chitimia-Dobler L, Straubinger RK.	Int J Mol Sci	10.3390/ijms24021100	2023
	Design and optimization of ciprofloxacin hydrochloride biodegradable 3D printed ocular inserts: Full factorial design and in-vitro and ex-vivo evaluations: Part II.	Alzahrani A, Youssef AAA, Nyavanandi D, Tripathi S, Bandari S, Majumdar S, Repka MA.	Int J Pharm	10.1016/j.ijpharm.2022.122533	2023
Metabolism	Active membrane transport and receptor proteins from bacteria.	Saidijam M, Bettaney KE, Szakonyi G, Psakis G, Shibayama K, Suzuki S, Clough JL, Blessie V, Abu-Bakr A, Baumberg S, Meuller J, Hoyle CK, Palmer SL, Butaye P, Walravens K, Patching SG, O'reilly J, Rutherford NG, Bill RM, Roper DI, Phillips-Jones MK, Henderson PJ.	Biochem Soc Trans	10.1042/bst0330867	2005
	Characterisation of the nitrile hydratase gene clusters of Rhodococcus erythropolis strains AJ270 and AJ300 and Microbacterium sp. AJ115 indicates horizontal gene transfer and reveals an insertion of IS1166.	O'Mahony R, Doran J, Coffey L, Cahill OJ, Black GW, O'Reilly C.	Antonie Van Leeuwenhoek	10.1007/s10482-004-3721-x	2005
	Design of Topical Ocular Ciprofloxacin Nanoemulsion for the Management of Bacterial Keratitis.	Youssef AAA, Cai C, Dudhipala N, Majumdar S.	Pharmaceuticals (Basel)	10.3390/ph14030210	2021
	Rhizobacterial effects on nickel extraction from soil and uptake by Alyssum murale.	Abou-Shanab RA, Angle JS, Delorme TA, Chaney RL, Van Berkum P, Moawad H, Ghanem K, Ghozlan HA.	New Phytol	10.1046/j.1469-8137.2003.00721.x	2003
Metabolism	Conformational cycle and ion-coupling mechanism of the Na+/hydantoin transporter Mhp1.	Kazmier K, Sharma S, Islam SM, Roux B, Mchaourab HS.	Proc Natl Acad Sci U S A	10.1073/pnas.1410431111	2014
Metabolism	Structure-function relationship of a plant NCS1 member--homology modeling and mutagenesis identified residues critical for substrate specificity of PLUTO, a nucleobase transporter from Arabidopsis.	Witz S, Panwar P, Schober M, Deppe J, Pasha FA, Lemieux MJ, Mohlmann T.	PLoS One	10.1371/journal.pone.0091343	2014
Pathogenicity	Diversity and Genetic Basis for Carbapenem Resistance in a Coastal Marine Environment.	Dewi DAPR, Gotz B, Thomas T.	Appl Environ Microbiol	10.1128/aem.02939-19	2020
Enzymology	Bacteria that Travel: The Quality of Aircraft Water.	Handschuh H, O'Dwyer J, Adley CC.	Int J Environ Res Public Health	10.3390/ijerph121113938	2015
Phylogeny	Reclassification of Brevibacterium oxydans (Chatelain and Second 1966) as Microbacterium oxydans comb. nov.	Schumann P, Rainey FA, Burghardt J, Stackebrandt E, Weiss N.	Int J Syst Bacteriol	10.1099/00207713-49-1-175	1999
Phylogeny	Union of the genera Microbacterium Orla-Jensen and Aureobacterium Collins et al. in a redefined genus Microbacterium.	Takeuchi M, Hatano K.	Int J Syst Bacteriol	10.1099/00207713-48-3-739	1998
Metabolism	A Novel SLC5A5 Variant Reveals the Crucial Role of Kinesin Light Chain 2 in Thyroid Hormonogenesis.	Martin M, Modenutti CP, Gil Rosas ML, Peyret V, Geysels RC, Bernal Barquero CE, Sobrero G, Munoz L, Signorino M, Testa G, Miras MB, Masini-Repiso AM, Calcaterra NB, Coux G, Carrasco N, Marti MA, Nicola JP.	J Clin Endocrinol Metab	10.1210/clinem/dgab283	2021
Metabolism	Allantoin transport protein, PucI, from Bacillus subtilis: evolutionary relationships, amplified expression, activity and specificity.	Ma P, Patching SG, Ivanova E, Baldwin JM, Sharples D, Baldwin SA, Henderson PJF.	Microbiology (Reading)	10.1099/mic.0.000266	2016
Metabolism	The alternating access mechanism of transport as observed in the sodium-hydantoin transporter Mhp1.	Weyand S, Shimamura T, Beckstein O, Sansom MS, Iwata S, Henderson PJ, Cameron AD.	J Synchrotron Radiat	10.1107/s0909049510032449	2011
Metabolism	The hydantoin transport protein from Microbacterium liquefaciens.	Suzuki S, Henderson PJ.	J Bacteriol	10.1128/jb.188.9.3329-3336.2006	2006
	An efficient strategy for small-scale screening and production of archaeal membrane transport proteins in Escherichia coli.	Ma P, Varela F, Magoch M, Silva AR, Rosario AL, Brito J, Oliveira TF, Nogly P, Pessanha M, Stelter M, Kletzin A, Henderson PJ, Archer M.	PLoS One	10.1371/journal.pone.0076913	2013
Metabolism	Discovering thiamine transporters as targets of chloroquine using a novel functional genomics strategy.	Huang Z, Srinivasan S, Zhang J, Chen K, Li Y, Li W, Quiocho FA, Pan X.	PLoS Genet	10.1371/journal.pgen.1003083	2012
Metabolism	Molecular basis of alternating access membrane transport by the sodium-hydantoin transporter Mhp1.	Shimamura T, Weyand S, Beckstein O, Rutherford NG, Hadden JM, Sharples D, Sansom MS, Iwata S, Henderson PJ, Cameron AD.	Science	10.1126/science.1186303	2010
Metabolism	Screening of candidate substrates and coupling ions of transporters by thermostability shift assays.	Majd H, King MS, Palmer SM, Smith AC, Elbourne LD, Paulsen IT, Sharples D, Henderson PJ, Kunji ER.	Elife	10.7554/elife.38821	2018
Enzymology	Crystallization and preliminary crystallographic studies of an active-site mutant hydantoin racemase from Sinorhizobium meliloti CECT4114.	Martinez-Rodriguez S, Gonzalez-Ramirez LA, Clemente-Jimenez JM, Rodriguez-Vico F, Las Heras-Vazquez FJ, Gavira JA, Garcia-Ruiz JM.	Acta Crystallogr Sect F Struct Biol Cryst Commun	10.1107/s1744309107066122	2008
Metabolism	Modeling, substrate docking, and mutational analysis identify residues essential for the function and specificity of a eukaryotic purine-cytosine NCS1 transporter.	Krypotou E, Kosti V, Amillis S, Myrianthopoulos V, Mikros E, Diallinas G.	J Biol Chem	10.1074/jbc.m112.400382	2012
	Structure and molecular mechanism of a nucleobase-cation-symport-1 family transporter.	Weyand S, Shimamura T, Yajima S, Suzuki S, Mirza O, Krusong K, Carpenter EP, Rutherford NG, Hadden JM, O'Reilly J, Ma P, Saidijam M, Patching SG, Hope RJ, Norbertczak HT, Roach PC, Iwata S, Henderson PJ, Cameron AD.	Science	10.1126/science.1164440	2008
Metabolism	Mutation of asparagine 76 in the center of glutamine transporter SNAT3 modulates substrate-induced conductances and Na+ binding.	Broer S, Schneider HP, Broer A, Deitmer JW.	J Biol Chem	10.1074/jbc.m109.031013	2009
	Antibacterial Activity of Endophytic Actinomycetes Isolated from the Medicinal Plant Vochysia divergens (Pantanal, Brazil).	Gos FMWR, Savi DC, Shaaban KA, Thorson JS, Aluizio R, Possiede YM, Rohr J, Glienke C.	Front Microbiol	10.3389/fmicb.2017.01642	2017
	Threo-beta-hydroxyornithine: a natural constituent of the peptidoglycan of Corynebacterium species Co 112.	Schleifer KH, Hayn I, Seidl HP, Firl J.	Arch Microbiol	10.1007/bf00407766	1983
	Classification of some coryneform bacteria in a new genus Aureobacterium.	Collins MD, Jones D, Keddie RM, Kroppenstedt RM, Schleifer KH.	Syst Appl Microbiol	10.1016/s0723-2020(83)80053-8	1983
Metabolism	Microbial degradation of microcystin in Florida's freshwaters.	Ramani A, Rein K, Shetty KG, Jayachandran K.	Biodegradation	10.1007/s10532-011-9484-y	2012
Metabolism	Molecular mechanism of ligand recognition by membrane transport protein, Mhp1.	Simmons KJ, Jackson SM, Brueckner F, Patching SG, Beckstein O, Ivanova E, Geng T, Weyand S, Drew D, Lanigan J, Sharples DJ, Sansom MS, Iwata S, Fishwick CW, Johnson AP, Cameron AD, Henderson PJ.	EMBO J	10.15252/embj.201387557	2014
	Characterization and performance of a toluene-degrading biofilm developed on pumice stones.	Di Lorenzo A, Varcamonti M, Parascandola P, Vignola R, Bernardi A, Sacceddu P, Sisto R, de Alteriis E.	Microb Cell Fact	10.1186/1475-2859-4-4	2005
Metabolism	Simulations of the alternating access mechanism of the sodium symporter Mhp1.	Adelman JL, Dale AL, Zwier MC, Bhatt D, Chong LT, Zuckerman DM, Grabe M.	Biophys J	10.1016/j.bpj.2011.09.061	2011
	Heavy metal tolerance (Cr, Ag AND Hg) in bacteria isolated from sewage.	Lima de Silva AA, de Carvalho MA, de Souza SA, Dias PM, da Silva Filho RG, de Meirelles Saramago CS, de Melo Bento CA, Hofer E.	Braz J Microbiol	10.1590/s1517-838220120004000047	2012
Metabolism	Yeast alpha-arrestin Art2 is the key regulator of ubiquitylation-dependent endocytosis of plasma membrane vitamin B1 transporters.	Savocco J, Nootens S, Afokpa W, Bausart M, Chen X, Villers J, Renard HF, Prevost M, Wattiez R, Morsomme P.	PLoS Biol	10.1371/journal.pbio.3000512	2019
	Allosteric regulation of the carbohydrate-binding ability of a novel conger eel galectin by D-mannoside.	Watanabe M, Nakamura O, Muramoto K, Ogawa T.	J Biol Chem	10.1074/jbc.m112.346213	2012
Metabolism	Role of soil rhizobacteria in phytoremediation of heavy metal contaminated soils.	Jing YD, He ZL, Yang XE.	J Zhejiang Univ Sci B	10.1631/jzus.2007.b0192	2007
	Impact of antibiotic resistance in the management of ocular infections: the role of current and future antibiotics.	Bertino JS.	Clin Ophthalmol	10.2147/opth.s5778	2009
Metabolism	Conserved glutamate residues Glu-343 and Glu-519 provide mechanistic insights into cation/nucleoside cotransport by human concentrative nucleoside transporter hCNT3.	Slugoski MD, Smith KM, Ng AML, Yao SYM, Karpinski E, Cass CE, Baldwin SA, Young JD.	J Biol Chem	10.1074/jbc.m109.009613	2009
Enzymology	Understanding transporter specificity and the discrete appearance of channel-like gating domains in transporters.	Diallinas G.	Front Pharmacol	10.3389/fphar.2014.00207	2014
	Surface microflora of four smear-ripened cheeses.	Mounier J, Gelsomino R, Goerges S, Vancanneyt M, Vandemeulebroecke K, Hoste B, Scherer S, Swings J, Fitzgerald GF, Cogan TM.	Appl Environ Microbiol	10.1128/aem.71.11.6489-6500.2005	2005

References

#8984	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 20638
#18766	Wink, J.: Compendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig .
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#20216	Curators of the JMRC: Jena Microbial Resource Collection (JMRC):
#40416	; Curators of the CIP;
#51372	Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 33091
#66792	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .
#67770	Japan Collection of Microorganism (JCM) ; Curators of the JCM;
#68368	Automatically annotated from API 20E .
#68379	Automatically annotated from API Coryne .
#68382	Automatically annotated from API zym .
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#120624	Collection of Institut Pasteur ; Curators of the CIP; CIP 102402
#124043	Isabel Schober, Julia Koblitz: Data extracted from sequence databases, automatically matched based on designation and taxonomy .
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Microbacterium liquefaciens (DSM 20638, ATCC 43647, NCIB 11509)

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API 20E

API coryne

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