Name: Methylobacillus pratensis F31
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 7236

Type strain:

Species: Methylobacillus pratensis

Strain Designation: F31

Variant: Isotype of BacDive ID 131099

Culture col. no.: DSM 16019, NCIMB 13994, VKM B-2247

Strain history: <- Y. A. Trotsenko, Inst. Biochem. Physiol. Microorg., RAS, Pushchino, Russia; F31 <- N. V. Doronina

NCBI tax ID(s): 233101 (species)

SI-ID 134297

NCIMB 13994, VKM B-2247, DSM 16019, DSM 14935

Other strains from Methylobacillus pratensis

M. pratensis F31, DSM 14935, NCIMB 13994, VKM ... (type strain)

Section

Methylobacillus pratensis F31 is an aerobe, mesophilic, Gram-negative bacterium that was isolated from meadow grass Poa trivialis L..

Gram-negative
motile
rod-shaped
aerobe
mesophilic
16S sequence
Bacteria

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Pseudomonadota*
	Class	*Betaproteobacteria*
	Order	*Nitrosomonadales*
	Family	*Methylophilaceae*
	Genus	*Methylobacillus*
	Species	**Methylobacillus pratensis**
	Full Scientific Name (LPSN)	Methylobacillus pratensis Doronina et al. 2004

Information on morphological and physiological properties Morphology

[Ref.: #30115]	Gram stain	negative

[Ref.: #30115]	Cell length	0.9-1.8 µm

[Ref.: #30115]	Cell width	0.5-0.7 µm

[Ref.: #30115]	Cell shape	rod-shaped

[Ref.: #30115]	Motility	yes

[Ref.: #30115]

Pigment production

Information on culture and growth conditions Culture and growth conditions

[Ref.: #6144]

Culture medium

MEDIUM K (DSMZ Medium 1122)

[Ref.: #6144]

Culture medium growth

[Ref.: #6144]

Culture medium link

Medium recipe at MediaDive

[Ref.: #6144]

Culture medium composition

expand / minimize

		Temperature
[Ref.: #6144]	growth	28 °C
[Ref.: #30115]	growth	10-37 °C
[Ref.: #30115]	optimum	27.5 °C

[Ref.: #6144]	Temperature range	mesophilic
[Ref.: #30115]	Temperature range	mesophilic

	pH	Kind of pH		pH
[Ref.: #30115]			optimum	7
[Ref.: #30115]			growth	5.5-8.5

Information on physiology and metabolism Physiology and metabolism

[Ref.: #30115]

Oxygen tolerance

aerobe

	Metabolite utilization	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #30115]		nitrate ChEBI	+	reduction

	Metabolite production	Metabolite	Production
[Ref.: #30115]		indole ChEBI	yes

	Physiological tests	Metabolite	Indole test
[Ref.: #30115]		indole ChEBI	+

	Enzyme	Enzyme activity	EC number
[Ref.: #30115]	catalase	+	1.11.1.6
[Ref.: #30115]	cytochrome oxidase	+	1.9.3.1
[Ref.: #30115]	urease	+	3.5.1.5

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #6144]	Sample type/isolated from	meadow grass Poa trivialis L.
[Ref.: #6144]	Host species	Poa trivialis
[Ref.: #6144]	Geographic location (country and/or sea, region)	Helsinki (city park)
[Ref.: #6144]	Country	Finland
[Ref.: #6144]	Country ISO 3 Code	FIN
[Ref.: #6144]	Continent	Europe
* marker position based on {}

Isolation sources categories

#Engineered	#Agriculture	#Meadow
#Host	#Plants	#Herbaceous plants (Grass,Crops)

What are isolation sources categories?

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #6144]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #6144]	Methylobacillus pratensis strain F31 16S ribosomal RNA gene, partial sequence	AY298905	1401	ENA	233101 tax ID

[Ref.: #6144]	GC-content	61.5 mol%	thermal denaturation, midpoint method (Tm)
[Ref.: #30115]	GC-content	61.5 mol%

Availability in culture collections External links

[Ref.: #6144]

Culture collection no.

DSM 16019, NCIMB 13994, VKM B-2247

[Ref.: #76688]

SI-ID 134297

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Methylobacillus pratensis sp. nov., a novel non-pigmented, aerobic, obligately methylotrophic bacterium isolated from meadow grass.	Doronina NV, Trotsenko YA, Kolganova TV, Tourova TP, Salkinoja-Salonen MS	Int J Syst Evol Microbiol	10.1099/ijs.0.02956-0	2004	*

References

#6144

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 16019

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#30115

Barberan A, Caceres Velazquez H, Jones S, Fierer N.: Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information. mSphere 2: 2017 ( DOI 10.1128/mSphere.00237-17 , PubMed 28776041 ) - originally annotated from #26470 (see below)

#76688

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID134297.1 )

#26470

IJSEM 1453 2004 ( DOI 10.1099/ijs.0.02956-0 , PubMed 15388694 )

* These data were automatically processed and therefore are not curated

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