Methylobacillus pratensis (DSM 16019, NCIMB 13994, VKM B-2247)

Name: Methylobacillus pratensis (DSM 16019, NCIMB 13994, VKM B-2247)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 7236

Status isotype of 131099

Type strain

Strain Designation F31

Culture col. no. DSM 16019 NCIMB 13994 VKM B-2247 F31

NCBI tax ID(s) 233101

Special Collections DSMZ Literature strains

History DSMZ <- Y. A. Trotsenko, Inst. Biochem. Physiol. Microorg., RAS, Pushchino, Russia; F31 <- N. V. Doronina

Links

Methylobacillus pratensis F31 is an aerobe, Gram-negative, motile bacterium that was isolated from meadow grass Poa trivialis L..

Gram-negative motile rod-shaped aerobe 16S sequence Bacteria

Name and taxonomic classification

SI-ID 134297

NCIMB 13994,VKM B-2247,DSM 16019,DSM 14935

@ref 20215

Last LPSN update 2026-05-29 08:26:36

Domain Bacteria

Phylum Pseudomonadota

Class Betaproteobacteria

Order Spirillales

Family Methylophilaceae

Genus Methylobacillus

Species Methylobacillus pratensis

Full scientific name Methylobacillus pratensis Doronina et al. 2004

BacDive ID	Other strains from **Methylobacillus pratensis** (1)	Type strain
131099	M. pratensis F31, DSM 14935, NCIMB 13994, VKM B-2247 (type strain)

Morphology

Cell morphology

@ref	Gram stain	Cell length	Cell width	Cell shape	Motility
30115	negative	0.9-1.8 µm	0.5-0.7 µm	rod-shaped

Pigmentation

30115

Productionno

Culture and growth conditions

Culture medium

@ref	Name	Growth	Medium link	Composition
6144	MEDIUM K (DSMZ Medium 1122)		Medium recipe at MediaDive	Name: MEDIUM K (DSMZ Medium 1122) Composition: Agar 20.0 g/l KH2PO4 2.0 g/l (NH4)2SO4 2.0 g/l NaCl 0.5 g/l MgSO4 x 7 H2O 0.125 g/l FeSO4 x 7 H2O 0.002 g/l Methanol Distilled water

Culture temp

@ref	Growth	Type	Temperature (°C)
6144	positive	growth	28
30115	positive	growth	10-37
30115	positive	optimum	27.5

Culture pH

@ref	Ability	Type	PH	PH range
30115	positive	optimum	7
30115	positive	growth	5.5-8.5	alkaliphile

Physiology and metabolism

Oxygen tolerance

30115

Oxygen toleranceaerobe

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
30115	17632 ChEBI	nitrate	+	reduction

Metabolite production

@ref	Chebi-ID	Metabolite	Production
30115	35581 ChEBI	indole

Metabolite tests

@ref	Chebi-ID	Metabolite	Indole test
30115	35581 ChEBI	indole	+

Enzymes

@ref	Value	Activity	Ec
30115	catalase	+	1.11.1.6
30115	cytochrome oxidase	+	1.9.3.1
30115	urease	+	3.5.1.5

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Engineered	#Agriculture	#Meadow
#Host	#Plants	#Herbaceous plants (Grass,Crops)

Isolation

@ref	Sample type	Host species	Geographic location	Country	Country ISO 3 Code	Continent
6144	meadow grass Poa trivialis L.	Poa trivialis	Helsinki (city park)	Finland	FIN	Europe

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
6144	1	Risk group (German classification) According to TRBA 466

Sequence information

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
6144	Methylobacillus pratensis strain F31 16S ribosomal RNA gene, partial sequence	AY298905	1401		233101
124043	Methylobacillus pratensis partial 16S rRNA gene, type strain DSM 16019T	LN901384	1536		233101

GC content

@ref	GC-content (mol%)	Method
6144	61.5	thermal denaturation, midpoint method (Tm)
30115	61.5

Genome-based predictions

Literature

Topic	Title	Authors	Journal	DOI	Year
	Diversity and distribution of iron-oxidising bacteria belonging to Gallionellaceae in different sites of a hydroelectric power plant.	de Paula RS, E Souza CC, Goncalves CAX, de Holanda Moura MV, Guanabens ACP, Andrade GR, Nascimento AMA, Cardoso AV, de Paula Reis M, Jorge EC.	Braz J Microbiol	10.1007/s42770-024-01245-w	2024
Phylogeny	Methylobacillus rhizosphaerae sp. nov., a novel plant-associated methylotrophic bacterium isolated from rhizosphere of red pepper.	Madhaiyan M, Poonguzhali S, Senthilkumar M, Pragatheswari D, Lee KC, Lee JS.	Antonie Van Leeuwenhoek	10.1007/s10482-012-9828-6	2013
Phylogeny	Methylobacillus pratensis sp. nov., a novel non-pigmented, aerobic, obligately methylotrophic bacterium isolated from meadow grass.	Doronina NV, Trotsenko YA, Kolganova TV, Tourova TP, Salkinoja-Salonen MS	Int J Syst Evol Microbiol	10.1099/ijs.0.02956-0	2004

References

#6144	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 16019
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#26470	IJSEM 1453 2004 ( DOI 10.1099/ijs.0.02956-0 , PubMed 15388694 )
#30115	Barberan A, Caceres Velazquez H, Jones S, Fierer N.: Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information. mSphere 2: 2017 ( DOI 10.1128/mSphere.00237-17 , PubMed 28776041 ) - originally annotated from #26470
#124043	Isabel Schober, Julia Koblitz: Data extracted from sequence databases, automatically matched based on designation and taxonomy .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Methylobacillus pratensis (DSM 16019, NCIMB 13994, VKM B-2247)

Name and taxonomic classification

Morphology

Cell morphology

Pigmentation

Culture and growth conditions

Culture medium

Culture temp

Culture pH

Physiology and metabolism

Oxygen tolerance

Metabolite utilization

Metabolite production

Metabolite tests

Enzymes

Isolation, sampling and environmental information

Isolation source categories

Isolation

Interaction and safety

Risk assessment

Sequence information

16S sequences

GC content

Genome-based predictions

Literature

Literature

References

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