Strain identifier
BacDive ID: 7236
Type strain: 
Species: Methylobacillus pratensis
Strain Designation: F31
Variant: Isotype of BacDive ID 131099
Strain history: <- Y. A. Trotsenko, Inst. Biochem. Physiol. Microorg., RAS, Pushchino, Russia; F31 <- N. V. Doronina
NCBI tax ID(s): 233101 (species)
version 8.1 (current version)
General
@ref: 6144
BacDive-ID: 7236
DSM-Number: 16019
keywords: 16S sequence, Bacteria, aerobe, mesophilic, Gram-negative, motile, rod-shaped
description: Methylobacillus pratensis F31 is an aerobe, mesophilic, Gram-negative bacterium that was isolated from meadow grass Poa trivialis L..
NCBI tax id
- NCBI tax id: 233101
- Matching level: species
strain history
- @ref: 6144
- history: <- Y. A. Trotsenko, Inst. Biochem. Physiol. Microorg., RAS, Pushchino, Russia; F31 <- N. V. Doronina
doi: 10.13145/bacdive7236.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/pseudomonadota
- domain: Bacteria
- phylum: Pseudomonadota
- class: Betaproteobacteria
- order: Nitrosomonadales
- family: Methylophilaceae
- genus: Methylobacillus
- species: Methylobacillus pratensis
- full scientific name: Methylobacillus pratensis Doronina et al. 2004
@ref: 6144
domain: Bacteria
phylum: Proteobacteria
class: Betaproteobacteria
order: Methylophilales
family: Methylophilaceae
genus: Methylobacillus
species: Methylobacillus pratensis
full scientific name: Methylobacillus pratensis Doronina et al. 2004
strain designation: F31
variant: Isotype of BacDive ID 131099
type strain: yes
Morphology
cell morphology
- @ref: 30115
- gram stain: negative
- cell length: 0.9-1.8 µm
- cell width: 0.5-0.7 µm
- cell shape: rod-shaped
- motility: yes
pigmentation
- @ref: 30115
- production: no
Culture and growth conditions
culture medium
- @ref: 6144
- name: MEDIUM K (DSMZ Medium 1122)
- growth: yes
- link: https://mediadive.dsmz.de/medium/1122
- composition: Name: MEDIUM K (DSMZ Medium 1122) Composition: Agar 20.0 g/l KH2PO4 2.0 g/l (NH4)2SO4 2.0 g/l NaCl 0.5 g/l MgSO4 x 7 H2O 0.125 g/l FeSO4 x 7 H2O 0.002 g/l Methanol Distilled water
culture temp
| @ref | growth | type | temperature | range |
|---|---|---|---|---|
| 6144 | positive | growth | 28 | mesophilic |
| 30115 | positive | growth | 10-37 | |
| 30115 | positive | optimum | 27.5 | mesophilic |
culture pH
| @ref | ability | type | pH | PH range |
|---|---|---|---|---|
| 30115 | positive | growth | 5.5-8.5 | alkaliphile |
| 30115 | positive | optimum | 7 |
Physiology and metabolism
oxygen tolerance
- @ref: 30115
- oxygen tolerance: aerobe
metabolite utilization
- @ref: 30115
- Chebi-ID: 17632
- metabolite: nitrate
- utilization activity: +
- kind of utilization tested: reduction
metabolite production
- @ref: 30115
- Chebi-ID: 35581
- metabolite: indole
- production: yes
metabolite tests
- @ref: 30115
- Chebi-ID: 35581
- metabolite: indole
- indole test: +
enzymes
| @ref | value | activity | ec |
|---|---|---|---|
| 30115 | catalase | + | 1.11.1.6 |
| 30115 | cytochrome oxidase | + | 1.9.3.1 |
| 30115 | urease | + | 3.5.1.5 |
Isolation, sampling and environmental information
isolation
- @ref: 6144
- sample type: meadow grass Poa trivialis L.
- host species: Poa trivialis
- geographic location: Helsinki (city park)
- country: Finland
- origin.country: FIN
- continent: Europe
isolation source categories
| Cat1 | Cat2 | Cat3 |
|---|---|---|
| #Engineered | #Agriculture | #Meadow |
| #Host | #Plants | #Herbaceous plants (Grass,Crops) |
Safety information
risk assessment
- @ref: 6144
- biosafety level: 1
- biosafety level comment: Risk group (German classification)
Sequence information
16S sequences
- @ref: 6144
- description: Methylobacillus pratensis strain F31 16S ribosomal RNA gene, partial sequence
- accession: AY298905
- length: 1401
- database: ena
- NCBI tax ID: 233101
GC content
| @ref | GC-content | method |
|---|---|---|
| 6144 | 61.5 | thermal denaturation, midpoint method (Tm) |
| 30115 | 61.5 |
External links
@ref: 6144
culture collection no.: DSM 16019, NCIMB 13994, VKM B-2247
straininfo link
- @ref: 76688
- straininfo: 134297
literature
- topic: Phylogeny
- Pubmed-ID: 15388694
- title: Methylobacillus pratensis sp. nov., a novel non-pigmented, aerobic, obligately methylotrophic bacterium isolated from meadow grass.
- authors: Doronina NV, Trotsenko YA, Kolganova TV, Tourova TP, Salkinoja-Salonen MS
- journal: Int J Syst Evol Microbiol
- DOI: 10.1099/ijs.0.02956-0
- year: 2004
- mesh: DNA, Bacterial/chemistry/isolation & purification, DNA, Ribosomal/chemistry/isolation & purification, Environmental Microbiology, Enzymes/analysis, Fatty Acids/analysis, Finland, Formaldehyde/metabolism, Genes, rRNA, Methylobacillus/*classification/*isolation & purification/physiology/ultrastructure, Molecular Sequence Data, Phylogeny, Poaceae/*microbiology, RNA, Bacterial/genetics, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA
- topic2: Enzymology
Reference
| @id | authors | catalogue | doi/url | title | ID_cross_reference | pubmed |
|---|---|---|---|---|---|---|
| 6144 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 16019) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-16019 | |||
| 20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |||
| 30115 | Barberan A, Caceres Velazquez H, Jones S, Fierer N. | 10.1128/mSphere.00237-17 | Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information | 26470 | 28776041 | |
| 76688 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID134297.1 | StrainInfo: A central database for resolving microbial strain identifiers |