Methylobacillus gramineus (DSM 23629, CCUG 59683, VKM B-2591)

Name: Methylobacillus gramineus (DSM 23629, CCUG 59683, VKM B-2591)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 7237

Type strain

Strain Designation Lap

Culture col. no. DSM 23629 CCUG 59683 VKM B-2591 Lap

NCBI tax ID(s) 755169

Special Collections DSMZ CCUG

History DSMZ <- Y. Trotsenko, VKM; Lap <- N. V. Doronina

Links

Methylobacillus gramineus Lap is an aerobe bacterium that was isolated from phyllosphere of silverweed .

aerobe genome sequence 16S sequence Bacteria

Name and taxonomic classification

SI-ID 402981

VKM B-2591,DSM 23629,CCUG 59683

@ref 20215

Last LPSN update 2026-05-29 08:26:36

Domain Bacteria

Phylum Pseudomonadota

Class Betaproteobacteria

Order Spirillales

Family Methylophilaceae

Genus Methylobacillus

Species Methylobacillus gramineus

Full scientific name Methylobacillus gramineus Gogleva et al. 2012

Morphology

Cell morphology

@ref	Gram stain	Confidence
125439	negative	98.511

Colony morphology

62076

Incubation period2-3 days

Culture and growth conditions

Culture medium

@ref	Name	Growth	Medium link	Composition
17301	MEDIUM K (DSMZ Medium 1122)		Medium recipe at MediaDive	Name: MEDIUM K (DSMZ Medium 1122) Composition: Agar 20.0 g/l KH2PO4 2.0 g/l (NH4)2SO4 2.0 g/l NaCl 0.5 g/l MgSO4 x 7 H2O 0.125 g/l FeSO4 x 7 H2O 0.002 g/l Methanol Distilled water

Culture temp

@ref	Growth	Type	Temperature (°C)
17301	positive	growth	29
62076	positive	growth	30

Physiology and metabolism

Oxygen tolerance

62076

Oxygen toleranceaerobe

Spore formation

@ref	Spore formation	Confidence
125439		99.873

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Host	#Plants	#Herbaceous plants (Grass,Crops)
#Host Body-Site	#Plant	#Leaf (Phyllosphere)

Isolation

@ref	Sample type	Host species	Geographic location	Country	Country ISO 3 Code	Continent	Sampling date
17301	phyllosphere of silverweed (Potentilla anserina L.)	Potentilla anserina	Moscow region, city park in Pushchino	Russia	RUS	Europe
62076	Phyllosphere of Potentilla anserina		Moscow region,Pushchino	Russia	RUS	Europe	2008

Taxonmaps

69479

Global distribution of 16S sequence GU937478 (>99% sequence identity) for Methylobacillus from Microbeatlas

Aquatic Samples	1076
Soil Samples	589
Animal Samples	2643
Plant Samples	789
Total Samples	5097

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
17301	1	Risk group (German classification) According to TRBA 466

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
124043	ASM2053267v1 assembly for Methylobacillus gramineus VKM B-2591	contig	GCA_020532675	755169.3	8064055726	755169	78.36

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
17301	Methylobacillus gramineus strain Lap 16S ribosomal RNA gene, partial sequence	GU937478	1406		755169

GC content

@ref	GC-content (mol%)	Method
17301	50.5	thermal denaturation, midpoint method (Tm)

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: ASM2053267v1 assembly for Methylobacillus gramineus VKM B-2591
NCBI: GCA_020532675

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	oxygen_tolerance	BacteriaNetⓘ	obligate aerobe	84.38	no
125439	gram_stain	BacteriaNetⓘ	negative	98.51	no
125439	motility	BacteriaNetⓘ	yes	75.29	no
125439	spore_formation	BacteriaNetⓘ	no	99.87	no

Literature

Topic	Title	Authors	Journal	DOI	Year
	Prerequisites for amplicon pyrosequencing of microbial methanol utilizers in the environment.	Kolb S, Stacheter A.	Front Microbiol	10.3389/fmicb.2013.00268	2013
Metabolism	Methylobacillus arboreus sp. nov., and Methylobacillus gramineus sp. nov., novel non-pigmented obligately methylotrophic bacteria associated with plants.	Gogleva AA, Kaparullina EN, Doronina NV, Trotsenko YA	Syst Appl Microbiol	10.1016/j.syapm.2011.03.005	2011

References

#17301	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 23629
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#62076	Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 59683
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#124043	Isabel Schober, Julia Koblitz: Data extracted from sequence databases, automatically matched based on designation and taxonomy .
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Methylobacillus gramineus (DSM 23629, CCUG 59683, VKM B-2591)

Name and taxonomic classification

Morphology

Cell morphology

Colony morphology

Culture and growth conditions

Culture medium

Culture temp

Physiology and metabolism

Oxygen tolerance

Spore formation

Isolation, sampling and environmental information

Isolation source categories

Isolation

Taxonmaps

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_020532675

16S sequences

GC content

Genome-based predictions

Predictions

Literature

Literature

References

BacDive

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