Methylohalobius crimeensis (DSM 16011, ATCC BAA-967, 10Ki)

Name: Methylohalobius crimeensis (DSM 16011, ATCC BAA-967, 10Ki)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 7205

Type strain

Strain Designation 10Ki

Culture col. no. DSM 16011 ATCC BAA-967 10Ki

NCBI tax ID(s) 1283300 244365

Special Collections DSMZ Literature strains

History <- P. F. Dunfield, MPI Inst. Terrestrial Microbiol., Marburg, Germany; 10Ki <- J. Heyer

Links

Methylohalobius crimeensis 10Ki is an aerobe, spore-forming, Gram-negative bacterium that was isolated from sediment from hypersaline Lake Kirleutskoe.

spore-forming Gram-negative motile rod-shaped aerobe genome sequence 16S sequence Bacteria

Name and taxonomic classification

SI-ID 398432

ATCC BAA-967,DSM 16011

@ref 20215

Last LPSN update 2026-02-09 11:19:24

Domain Bacteria

Phylum Pseudomonadota

Class Gammaproteobacteria

Order Methylococcales

Family Methylothermaceae

Genus Methylohalobius

Species Methylohalobius crimeensis

Full scientific name Methylohalobius crimeensis Heyer et al. 2005

Morphology

Cell morphology

@ref	Gram stain	Cell length	Cell width	Cell shape	Confidence
31286	negative	2.1 µm	1 µm	rod-shaped
125438	negative				98
125439	negative				92.8

Pigmentation

31286

Productionno

Culture and growth conditions

Culture medium

@ref	Name	Growth	Medium link	Composition
6149	HALOPHILIC METHANOTROPHIC BACTERIUM MEDIUM (DSMZ Medium 1156)		Medium recipe at MediaDive	Name: HALOPHILIC METHANOTROPHIC BACTERIUM MEDIUM (DSMZ Medium 1156) Composition: NaCl 65.0 g/l Na2HPO4 x 2 H2O 0.7 g/l NH4Cl 0.5 g/l KH2PO4 0.3 g/l MgSO4 x 7 H2O 0.1 g/l CaCl2 x 6 H2O 0.01 g/l FeSO4 x 7 H2O 0.005 g/l ZnSO4 x 7 H2O 0.00044 g/l CuSO4 x 5 H2O 0.0002 g/l MnSO4 x 2 H2O 0.00017 g/l H3BO3 0.0001 g/l CoCl2 x 6 H2O 8e-05 g/l Na2MoO4 x 2 H2O 6e-05 g/l Distilled water

Culture temp

@ref	Growth	Type	Temperature (°C)
6149	positive	growth	30
31286	positive	growth	15-42
31286	positive	optimum	30

Culture pH

@ref	Ability	Type	PH
31286	positive	optimum	7
31286	positive	growth	6.5-7.5

Physiology and metabolism

Oxygen tolerance

31286

Oxygen toleranceaerobe

Spore formation

@ref	Spore formation	Confidence
31286
125439		90.5

Halophily

@ref	Salt	Growth	Tested relation	Concentration
31286	NaCl	positive	growth	0.2-2.5 %
31286	NaCl	positive	optimum	1.25 %

Observation

31286

Observationaggregates in chains

Metabolite production

@ref	Chebi-ID	Metabolite	Production
31286	16183 ChEBI	methane

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Environmental	#Aquatic	#Lake (large)
#Environmental	#Aquatic	#Sediment
#Condition	#Saline	-

Isolation

@ref	Sample type	Geographic location	Country	Country ISO 3 Code	Continent
6149	sediment from hypersaline Lake Kirleutskoe	Crimean Peninsula	Ukraine	UKR	Europe

Taxonmaps

69479

Global distribution of 16S sequence AJ581837 (>99% sequence identity) for Methylohalobius crimeensis subclade from Microbeatlas

Aquatic Samples	148
Soil Samples	37
Animal Samples	428
Plant Samples	25
Total Samples	638

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
6149	1	Risk group (German classification) According to TRBA 466

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
66792	ASM42146v1 assembly for Methylohalobius crimeensis 10Ki	contig	GCA_000421465	1283300.3	2524614657	1283300	73.56

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
6149	Methylohalobius crimeensis partial 16S rRNA gene, strain 10Ki	AJ581837	1462		1283300

GC content

6149

GC-content (mol%)58.7

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: Methylohalobius crimeensis 10Ki
NCBI: GCA_000421465

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	oxygen_tolerance	BacteriaNetⓘ	anaerobe	63.70	no
125439	gram_stain	BacteriaNetⓘ	negative	92.80	no
125439	motility	BacteriaNetⓘ	yes	72.10	no
125439	spore_formation	BacteriaNetⓘ	no	90.50	no

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Methylohalobius crimeensis 10Ki
PATRIC: 1283300.3

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	no	98.00	yes
125438	anaerobic	anaerobicⓘ	no	85.58	yes
125438	spore-forming	spore-formingⓘ	no	87.17	no
125438	aerobic	aerobicⓘ	yes	67.07	yes
125438	thermophilic	thermophileⓘ	no	91.65	yes
125438	flagellated	motile2+ⓘ	yes	62.34	no

Literature

Topic	Title	Authors	Journal	DOI	Year
	Safety and efficacy of a feed additive consisting of xylanase (produced with Komagataella phaffii DSM 25376) and beta-glucanase (produced with Komagataella phaffii DSM 26469) (ENZY CARBOPLUS®) for all poultry (Kaesler Nutrition GmbH).	EFSA Panel on Additives and Products or Substances used in Animal Feed (FEEDAP), Villa RE, Azimonti G, Bonos E, Christensen H, Durjava M, Dusemund B, Gehring R, Glandorf B, Kouba M, Lopez-Alonso M, Marcon F, Nebbia C, Pechova A, Prieto-Maradona M, Rohe I, Theodoridou K, Mayo B, Herman L, Anguita M, Cionci NB, Innocenti ML, Ortuno J, Pettenati E.	EFSA J	10.2903/j.efsa.2025.9344	2025
	Biocatalytic Syntheses of Antiplatelet Metabolites of the Thienopyridines Clopidogrel and Prasugrel Using Fungal Peroxygenases.	Kiebist J, Schmidtke KU, Schramm M, Konig R, Quint S, Kohlmann J, Zuhse R, Ullrich R, Hofrichter M, Scheibner K.	J Fungi (Basel)	10.3390/jof7090752	2021
Phylogeny	Diversity and Habitat Preferences of Cultivated and Uncultivated Aerobic Methanotrophic Bacteria Evaluated Based on pmoA as Molecular Marker.	Knief C.	Front Microbiol	10.3389/fmicb.2015.01346	2015
Metabolism	The coprophilous mushroom Coprinus radians secretes a haloperoxidase that catalyzes aromatic peroxygenation.	Anh DH, Ullrich R, Benndorf D, Svatos A, Muck A, Hofrichter M.	Appl Environ Microbiol	10.1128/aem.00026-07	2007
	Methylomarinovum tepidoasis sp. nov., a moderately thermophilic methanotroph of the family Methylothermaceae isolated from a deep-sea hydrothermal field.	Hirayama H, Takaki Y, Abe M, Miyazaki M, Uematsu K, Matsui Y, Takai K.	Int J Syst Evol Microbiol	10.1099/ijsem.0.006288	2024
Phylogeny	Methylohalobius crimeensis gen. nov., sp. nov., a moderately halophilic, methanotrophic bacterium isolated from hypersaline lakes of Crimea.	Heyer J, Berger U, Hardt M, Dunfield PF	Int J Syst Evol Microbiol	10.1099/ijs.0.63213-0	2005

References

#6149	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 16011
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#27605	IJSEM 1817 2005 ( DOI 10.1099/ijs.0.63213-0 , PubMed 16166672 )
#31286	Barberan A, Caceres Velazquez H, Jones S, Fierer N.: Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information. mSphere 2: 2017 ( DOI 10.1128/mSphere.00237-17 , PubMed 28776041 ) - originally annotated from #27605
#66792	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

Rate our new design

Content

About us

Methylohalobius crimeensis (DSM 16011, ATCC BAA-967, 10Ki)

Name and taxonomic classification

Morphology

Cell morphology

Pigmentation

Culture and growth conditions

Culture medium

Culture temp

Culture pH

Physiology and metabolism

Oxygen tolerance

Spore formation

Halophily

Observation

Metabolite production

Isolation, sampling and environmental information

Isolation source categories

Isolation

Taxonmaps

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_000421465

16S sequences

GC content

Genome-based predictions

Predictions

Literature

Literature

References

BacDive

Help

Social Media