Name: Methylohalobius crimeensis 10Ki
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 7205

Type strain:

Species: Methylohalobius crimeensis

Strain Designation: 10Ki

Culture col. no.: DSM 16011, ATCC BAA-967

Strain history: <- P. F. Dunfield, MPI Inst. Terrestrial Microbiol., Marburg, Germany; 10Ki <- J. Heyer

NCBI tax ID(s): 1283300 (strain), 244365 (species)

SI-ID 398432

DSM 16011

Section

Methylohalobius crimeensis 10Ki is an aerobe, spore-forming, mesophilic bacterium that was isolated from sediment from hypersaline Lake Kirleutskoe.

Gram-negative
motile
rod-shaped
spore-forming
aerobe
mesophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Pseudomonadota*
	Class	*Gammaproteobacteria*
	Order	*Methylococcales*
	Family	*Methylothermaceae*
	Genus	*Methylohalobius*
	Species	**Methylohalobius crimeensis**
	Full Scientific Name (LPSN)	Methylohalobius crimeensis Heyer et al. 2005

Information on morphological and physiological properties Morphology

[Ref.: #31286]	Gram stain	negative

[Ref.: #31286]	Cell length	2.1 µm

[Ref.: #31286]	Cell width	1 µm

[Ref.: #31286]	Cell shape	rod-shaped

[Ref.: #31286]	Motility	yes

[Ref.: #31286]

Pigment production

Information on culture and growth conditions Culture and growth conditions

[Ref.: #6149]

Culture medium

HALOPHILIC METHANOTROPHIC BACTERIUM MEDIUM (DSMZ Medium 1156)

[Ref.: #6149]

Culture medium growth

[Ref.: #6149]

Culture medium link

Medium recipe at MediaDive

[Ref.: #6149]

Culture medium composition

expand / minimize

		Temperature
[Ref.: #6149]	growth	30 °C
[Ref.: #31286]	growth	15-42 °C
[Ref.: #31286]	optimum	30 °C

[Ref.: #6149]	Temperature range	mesophilic
[Ref.: #31286]	Temperature range	mesophilic

	pH	Kind of pH		pH
[Ref.: #31286]			optimum	7
[Ref.: #31286]			growth	6.5-7.5

Information on physiology and metabolism Physiology and metabolism

[Ref.: #31286]

Oxygen tolerance

aerobe

[Ref.: #31286]

Ability of spore formation

yes

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #31286]		NaCl		growth	0.2-2.5	%
[Ref.: #31286]		NaCl		optimum	1.25	%

[Ref.: #31286]

Observation

aggregates in chains

	Metabolite production	Metabolite	Production
[Ref.: #31286]		methane ChEBI	yes

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #6149]	Sample type/isolated from	sediment from hypersaline Lake Kirleutskoe
[Ref.: #6149]	Geographic location (country and/or sea, region)	Crimean Peninsula
[Ref.: #6149]	Country	Ukraine
[Ref.: #6149]	Country ISO 3 Code	UKR
[Ref.: #6149]	Continent	Europe
* marker position based on {}

Isolation sources categories

#Environmental	#Aquatic	#Lake (large)
#Environmental	#Aquatic	#Sediment
#Condition	#Saline	-

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence AJ581837 (>99% sequence identity) for Methylohalobius crimeensis subclade from Microbeatlas

Aquatic Samples	148
Soil Samples	37
Animal Samples	428
Plant Samples	25
Total Samples	638

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #6149]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #6149]	Methylohalobius crimeensis partial 16S rRNA gene, strain 10Ki	AJ581837	1462	ENA	1283300 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Methylohalobius crimeensis 10Ki	GCA_000421465	contig	GenBank	1283300 tax ID	*
[Ref.: #66792]	Methylohalobius crimeensis 10Ki	1283300.3	wgs	PATRIC	1283300 tax ID	*
[Ref.: #66792]	Methylohalobius crimeensis 10Ki	2524614657	draft	JGI IMG/M	1283300 tax ID	*

[Ref.: #6149]

GC-content

58.7 mol%

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Methylohalobius crimeensis 10Ki PATRIC: 1283300.3
[Ref.: #69480]	motile	yes	88.193	yes
[Ref.: #69480]	gram-positive	no	98.101	no
[Ref.: #69480]	anaerobic	no	92.994	no
[Ref.: #69480]	aerobic	no	50	no
[Ref.: #69480]	halophile	no	70.846	yes
[Ref.: #69480]	spore-forming	no	78.135	yes
[Ref.: #69480]	thermophile	no	88.343	no
[Ref.: #69480]	glucose-util	yes	68.298	no
[Ref.: #69480]	flagellated	no	69.132	no
[Ref.: #69480]	glucose-ferment	no	85.165	no

Availability in culture collections External links

[Ref.: #6149]

Culture collection no.

DSM 16011, ATCC BAA-967

[Ref.: #76658]

SI-ID 398432

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Methylohalobius crimeensis gen. nov., sp. nov., a moderately halophilic, methanotrophic bacterium isolated from hypersaline lakes of Crimea.	Heyer J, Berger U, Hardt M, Dunfield PF	Int J Syst Evol Microbiol	10.1099/ijs.0.63213-0	2005	*

References

#6149

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 16011

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#31286

Barberan A, Caceres Velazquez H, Jones S, Fierer N.: Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information. mSphere 2: 2017 ( DOI 10.1128/mSphere.00237-17 , PubMed 28776041 ) - originally annotated from #27605 (see below)

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#76658

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID398432.1 )

#27605

IJSEM 1817 2005 ( DOI 10.1099/ijs.0.63213-0 , PubMed 16166672 )

* These data were automatically processed and therefore are not curated

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