Name: Methylotuvimicrobium alcaliphilum 20Z
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 7206

Type strain:

Species: Methylotuvimicrobium alcaliphilum

Strain Designation: 20Z

Culture col. no.: DSM 19304, NCIMB 14124, VKM B-2133

Strain history: <- Y. A. Trotsenko, Inst. Biochem. Physiol. Microorg., RAS, Pushchino, Russia; 20Z <- V. N. Khmelenina

NCBI tax ID(s): 271065 (species)

SI-ID 343457

NCIMB 14124, VKM B-2133, DSM 19304, LMG 27836

Section

Methylotuvimicrobium alcaliphilum 20Z is an aerobe, mesophilic, Gram-negative bacterium that was isolated from surface sediment of the highly alkaline soda lake Shara-Nur.

Gram-negative
motile
rod-shaped
aerobe
mesophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Pseudomonadota*
	Class	*Gammaproteobacteria*
	Order	*Methylococcales*
	Family	[Methylococcales, not assigned to family]
	Genus	*Methylotuvimicrobium*
	Species	**Methylotuvimicrobium alcaliphilum**
	Full Scientific Name (LPSN)	Methylotuvimicrobium alcaliphilum (Kalyuzhnaya et al. 2008) Orata et al. 2019

	Synonym	Methylomicrobium alcaliphilum
	Synonym	"Methylobacter* alcaliphilus"*

Information on morphological and physiological properties Morphology

[Ref.: #32326]	Gram stain	negative

[Ref.: #32326]	Cell length	2.5 µm

[Ref.: #32326]	Cell width	1.25 µm

[Ref.: #32326]	Cell shape	rod-shaped

[Ref.: #32326]	Motility	yes

Information on culture and growth conditions Culture and growth conditions

[Ref.: #8007]

Culture medium

METHYLOMICROBIUM MEDIUM (DSMZ Medium 1180)

[Ref.: #8007]

Culture medium growth

[Ref.: #8007]

Culture medium link

Medium recipe at MediaDive

[Ref.: #8007]

Culture medium composition

expand / minimize

	Temperatures	Kind of temperature		Temperature
[Ref.: #8007]			growth	28 °C

[Ref.: #8007]

Temperature range

mesophilic

	pH	Kind of pH		pH
[Ref.: #32326]			optimum	9
[Ref.: #32326]			growth	7.2-9.5

Information on physiology and metabolism Physiology and metabolism

[Ref.: #32326]

Oxygen tolerance

aerobe

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #32326]		NaCl		growth	<8.7	%

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #8007]	Sample type/isolated from	surface sediment of the highly alkaline soda lake Shara-Nur
[Ref.: #8007]	Geographic location (country and/or sea, region)	Siberia, Tuva
[Ref.: #8007]	Country	Russia
[Ref.: #8007]	Country ISO 3 Code	RUS
[Ref.: #8007]	Continent	Asia
* marker position based on {}

Isolation sources categories

#Environmental	#Aquatic	#Lake (large)
#Environmental	#Aquatic	#Sediment
#Condition	#Alkaline	-
#Condition	#Saline	-

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence EF495157 (>99% sequence identity) for Methylotuvimicrobium from Microbeatlas

Aquatic Samples	562
Soil Samples	170
Animal Samples	118
Plant Samples	5
Total Samples	855

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #8007]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #8007]	Methylomicrobium alcaliphilum strain 20Z 16S ribosomal RNA gene, partial sequence	EF495157	1395	ENA	1091494 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Methylotuvimicrobium alcaliphilum 20Z	GCA_000968535	complete	GenBank	1091494 tax ID	*

[Ref.: #8007]	GC-content	47.9 mol%	thermal denaturation, midpoint method (Tm)
[Ref.: #32326]	GC-content	47.9 mol%

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Methylotuvimicrobium alcaliphilum 20Z 20Z NCBI: GCA_000968535.1
[Ref.: #69480]	motile	yes	87.599	yes
[Ref.: #69480]	flagellated	yes	54.047	no
[Ref.: #69480]	gram-positive	no	99.075	no
[Ref.: #69480]	anaerobic	no	97.413	yes
[Ref.: #69480]	aerobic	yes	88.599	yes
[Ref.: #69480]	halophile	no	61.121	yes
[Ref.: #69480]	spore-forming	no	88.97	no
[Ref.: #69480]	thermophile	no	99.348	no
[Ref.: #69480]	glucose-util	yes	66.991	no
[Ref.: #69480]	glucose-ferment	no	84.641	no

Availability in culture collections External links

[Ref.: #8007]

Culture collection no.

DSM 19304, NCIMB 14124, VKM B-2133

[Ref.: #76659]

SI-ID 343457

Literature:

Topic	Title	Authors	Journal	DOI	Year
Metabolism	Adaptive evolution of Methylotuvimicrobium alcaliphilum to grow in the presence of rhamnolipids improves fatty acid and rhamnolipid production from CH4.	Awasthi D, Tang YH, Amer B, Baidoo EEK, Gin J, Chen Y, Petzold CJ, Kalyuzhnaya M, Singer SW	J Ind Microbiol Biotechnol	10.1093/jimb/kuac002	2022	*
Phylogeny	Classification of halo(alkali)philic and halo(alkali)tolerant methanotrophs provisionally assigned to the genera Methylomicrobium and Methylobacter and emended description of the genus Methylomicrobium.	Kalyuzhnaya MG, Khmelenina V, Eshinimaev B, Sorokin D, Fuse H, Lidstrom M, Trotsenko Y	Int J Syst Evol Microbiol	10.1099/ijs.0.65317-0	2008	*

References

#8007

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 19304

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#32326

Barberan A, Caceres Velazquez H, Jones S, Fierer N.: Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information. mSphere 2: 2017 ( DOI 10.1128/mSphere.00237-17 , PubMed 28776041 ) - originally annotated from #28560 (see below)

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#76659

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID343457.1 )

#28560

IJSEM 591 2008 ( DOI 10.1099/ijs.0.65317-0 , PubMed 18319461 )

* These data were automatically processed and therefore are not curated

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Methylotuvimicrobium alcaliphilum 20Z 20Z

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