Strain identifier
BacDive ID: 7205
Type strain:
Species: Methylohalobius crimeensis
Strain Designation: 10Ki
Strain history: <- P. F. Dunfield, MPI Inst. Terrestrial Microbiol., Marburg, Germany; 10Ki <- J. Heyer
NCBI tax ID(s): 1283300 (strain), 244365 (species)
General
@ref: 6149
BacDive-ID: 7205
DSM-Number: 16011
keywords: genome sequence, 16S sequence, Bacteria, aerobe, spore-forming, mesophilic, Gram-negative, motile, rod-shaped
description: Methylohalobius crimeensis 10Ki is an aerobe, spore-forming, mesophilic bacterium that was isolated from sediment from hypersaline Lake Kirleutskoe.
NCBI tax id
NCBI tax id | Matching level |
---|---|
244365 | species |
1283300 | strain |
strain history
- @ref: 6149
- history: <- P. F. Dunfield, MPI Inst. Terrestrial Microbiol., Marburg, Germany; 10Ki <- J. Heyer
doi: 10.13145/bacdive7205.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/pseudomonadota
- domain: Bacteria
- phylum: Pseudomonadota
- class: Gammaproteobacteria
- order: Methylococcales
- family: Methylothermaceae
- genus: Methylohalobius
- species: Methylohalobius crimeensis
- full scientific name: Methylohalobius crimeensis Heyer et al. 2005
@ref: 6149
domain: Bacteria
phylum: Proteobacteria
class: Gammaproteobacteria
order: Methylococcales
family: Methylothermaceae
genus: Methylohalobius
species: Methylohalobius crimeensis
full scientific name: Methylohalobius crimeensis Heyer et al. 2005
strain designation: 10Ki
type strain: yes
Morphology
cell morphology
- @ref: 31286
- gram stain: negative
- cell length: 2.1 µm
- cell width: 1 µm
- cell shape: rod-shaped
- motility: yes
pigmentation
- @ref: 31286
- production: no
Culture and growth conditions
culture medium
- @ref: 6149
- name: HALOPHILIC METHANOTROPHIC BACTERIUM MEDIUM (DSMZ Medium 1156)
- growth: yes
- link: https://mediadive.dsmz.de/medium/1156
- composition: Name: HALOPHILIC METHANOTROPHIC BACTERIUM MEDIUM (DSMZ Medium 1156) Composition: NaCl 65.0 g/l Na2HPO4 x 2 H2O 0.7 g/l NH4Cl 0.5 g/l KH2PO4 0.3 g/l MgSO4 x 7 H2O 0.1 g/l CaCl2 x 6 H2O 0.01 g/l FeSO4 x 7 H2O 0.005 g/l ZnSO4 x 7 H2O 0.00044 g/l CuSO4 x 5 H2O 0.0002 g/l MnSO4 x 2 H2O 0.00017 g/l H3BO3 0.0001 g/l CoCl2 x 6 H2O 8e-05 g/l Na2MoO4 x 2 H2O 6e-05 g/l Distilled water
culture temp
@ref | growth | type | temperature | range |
---|---|---|---|---|
6149 | positive | growth | 30 | mesophilic |
31286 | positive | growth | 15-42 | |
31286 | positive | optimum | 30 | mesophilic |
culture pH
@ref | ability | type | pH |
---|---|---|---|
31286 | positive | growth | 6.5-7.5 |
31286 | positive | optimum | 7 |
Physiology and metabolism
oxygen tolerance
- @ref: 31286
- oxygen tolerance: aerobe
spore formation
- @ref: 31286
- spore formation: yes
halophily
@ref | salt | growth | tested relation | concentration |
---|---|---|---|---|
31286 | NaCl | positive | growth | 0.2-2.5 % |
31286 | NaCl | positive | optimum | 1.25 % |
observation
- @ref: 31286
- observation: aggregates in chains
metabolite production
- @ref: 31286
- Chebi-ID: 16183
- metabolite: methane
- production: yes
Isolation, sampling and environmental information
isolation
- @ref: 6149
- sample type: sediment from hypersaline Lake Kirleutskoe
- geographic location: Crimean Peninsula
- country: Ukraine
- origin.country: UKR
- continent: Europe
isolation source categories
Cat1 | Cat2 | Cat3 |
---|---|---|
#Environmental | #Aquatic | #Lake (large) |
#Environmental | #Aquatic | #Sediment |
#Condition | #Saline |
taxonmaps
- @ref: 69479
- File name: preview.99_4152.png
- url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_16895;96_2102;97_2533;98_3135;99_4152&stattab=map
- Last taxonomy: Methylohalobius crimeensis subclade
- 16S sequence: AJ581837
- Sequence Identity:
- Total samples: 638
- soil counts: 37
- aquatic counts: 148
- animal counts: 428
- plant counts: 25
Safety information
risk assessment
- @ref: 6149
- biosafety level: 1
- biosafety level comment: Risk group (German classification)
Sequence information
16S sequences
- @ref: 6149
- description: Methylohalobius crimeensis partial 16S rRNA gene, strain 10Ki
- accession: AJ581837
- length: 1462
- database: ena
- NCBI tax ID: 1283300
Genome sequences
@ref | description | accession | assembly level | database | NCBI tax ID |
---|---|---|---|---|---|
66792 | Methylohalobius crimeensis 10Ki | GCA_000421465 | contig | ncbi | 1283300 |
66792 | Methylohalobius crimeensis 10Ki | 1283300.3 | wgs | patric | 1283300 |
66792 | Methylohalobius crimeensis 10Ki | 2524614657 | draft | img | 1283300 |
GC content
- @ref: 6149
- GC-content: 58.7
Genome-based predictions
predictions
trait | prediction | confidence | training_data |
---|---|---|---|
motile | yes | 88.193 | yes |
gram-positive | no | 98.101 | no |
anaerobic | no | 92.994 | no |
aerobic | no | 50 | no |
halophile | no | 70.846 | yes |
spore-forming | no | 78.135 | yes |
thermophile | no | 88.343 | no |
glucose-util | yes | 68.298 | no |
flagellated | no | 69.132 | no |
glucose-ferment | no | 85.165 | no |
External links
@ref: 6149
culture collection no.: DSM 16011, ATCC BAA 967
straininfo link
- @ref: 76658
- straininfo: 398432
literature
- topic: Phylogeny
- Pubmed-ID: 16166672
- title: Methylohalobius crimeensis gen. nov., sp. nov., a moderately halophilic, methanotrophic bacterium isolated from hypersaline lakes of Crimea.
- authors: Heyer J, Berger U, Hardt M, Dunfield PF
- journal: Int J Syst Evol Microbiol
- DOI: 10.1099/ijs.0.63213-0
- year: 2005
- mesh: Bacterial Typing Techniques, Culture Media, DNA, Bacterial/analysis, Fatty Acids/analysis, Fresh Water/*microbiology, Genes, rRNA, Methane/*metabolism, Methylococcaceae/*classification/genetics/growth & development/isolation & purification, Molecular Sequence Data, Phylogeny, RNA, Ribosomal, 16S, *Sodium Chloride, Ukraine
- topic2: Cultivation
Reference
@id | authors | catalogue | doi/url | title | ID_cross_reference | pubmed |
---|---|---|---|---|---|---|
6149 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 16011) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-16011 | |||
20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |||
31286 | Barberan A, Caceres Velazquez H, Jones S, Fierer N. | 10.1128/mSphere.00237-17 | Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information | 27605 | 28776041 | |
66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |||
69479 | João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich. | https://microbeatlas.org/ | MicrobeAtlas 1.0 beta | |||
76658 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID398432.1 | StrainInfo: A central database for resolving microbial strain identifiers |