Strain identifier

BacDive ID: 7205

Type strain: Yes

Species: Methylohalobius crimeensis

Strain Designation: 10Ki

Strain history: <- P. F. Dunfield, MPI Inst. Terrestrial Microbiol., Marburg, Germany; 10Ki <- J. Heyer

For citation purpose refer to the digital object identifier (doi) of the current version.
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General

@ref: 6149

BacDive-ID: 7205

DSM-Number: 16011

keywords: genome sequence, 16S sequence, Bacteria, aerobe, spore-forming, mesophilic, Gram-negative, motile, rod-shaped

description: Methylohalobius crimeensis 10Ki is an aerobe, spore-forming, mesophilic bacterium that was isolated from sediment from hypersaline Lake Kirleutskoe.

NCBI tax id

NCBI tax idMatching level
244365species
1283300strain

strain history

  • @ref: 6149
  • history: <- P. F. Dunfield, MPI Inst. Terrestrial Microbiol., Marburg, Germany; 10Ki <- J. Heyer

doi: 10.13145/bacdive7205.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Gammaproteobacteria
  • order: Methylococcales
  • family: Methylothermaceae
  • genus: Methylohalobius
  • species: Methylohalobius crimeensis
  • full scientific name: Methylohalobius crimeensis Heyer et al. 2005

@ref: 6149

domain: Bacteria

phylum: Proteobacteria

class: Gammaproteobacteria

order: Methylococcales

family: Methylothermaceae

genus: Methylohalobius

species: Methylohalobius crimeensis

full scientific name: Methylohalobius crimeensis Heyer et al. 2005

strain designation: 10Ki

type strain: yes

Morphology

cell morphology

  • @ref: 31286
  • gram stain: negative
  • cell length: 2.1 µm
  • cell width: 1 µm
  • cell shape: rod-shaped
  • motility: yes

pigmentation

  • @ref: 31286
  • production: no

Culture and growth conditions

culture medium

  • @ref: 6149
  • name: HALOPHILIC METHANOTROPHIC BACTERIUM MEDIUM (DSMZ Medium 1156)
  • growth: yes
  • link: https://mediadive.dsmz.de/medium/1156
  • composition: Name: HALOPHILIC METHANOTROPHIC BACTERIUM MEDIUM (DSMZ Medium 1156) Composition: NaCl 65.0 g/l Na2HPO4 x 2 H2O 0.7 g/l NH4Cl 0.5 g/l KH2PO4 0.3 g/l MgSO4 x 7 H2O 0.1 g/l CaCl2 x 6 H2O 0.01 g/l FeSO4 x 7 H2O 0.005 g/l ZnSO4 x 7 H2O 0.00044 g/l CuSO4 x 5 H2O 0.0002 g/l MnSO4 x 2 H2O 0.00017 g/l H3BO3 0.0001 g/l CoCl2 x 6 H2O 8e-05 g/l Na2MoO4 x 2 H2O 6e-05 g/l Distilled water

culture temp

@refgrowthtypetemperaturerange
6149positivegrowth30mesophilic
31286positivegrowth15-42
31286positiveoptimum30mesophilic

culture pH

@refabilitytypepH
31286positivegrowth6.5-7.5
31286positiveoptimum7

Physiology and metabolism

oxygen tolerance

  • @ref: 31286
  • oxygen tolerance: aerobe

spore formation

  • @ref: 31286
  • spore formation: yes

halophily

@refsaltgrowthtested relationconcentration
31286NaClpositivegrowth0.2-2.5 %
31286NaClpositiveoptimum1.25 %

observation

  • @ref: 31286
  • observation: aggregates in chains

metabolite production

  • @ref: 31286
  • Chebi-ID: 16183
  • metabolite: methane
  • production: yes

Isolation, sampling and environmental information

isolation

  • @ref: 6149
  • sample type: sediment from hypersaline Lake Kirleutskoe
  • geographic location: Crimean Peninsula
  • country: Ukraine
  • origin.country: UKR
  • continent: Europe

isolation source categories

Cat1Cat2Cat3
#Environmental#Aquatic#Lake (large)
#Environmental#Aquatic#Sediment
#Condition#Saline

taxonmaps

  • @ref: 69479
  • File name: preview.99_4152.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_16895;96_2102;97_2533;98_3135;99_4152&stattab=map
  • Last taxonomy: Methylohalobius crimeensis subclade
  • 16S sequence: AJ581837
  • Sequence Identity:
  • Total samples: 638
  • soil counts: 37
  • aquatic counts: 148
  • animal counts: 428
  • plant counts: 25

Safety information

risk assessment

  • @ref: 6149
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 6149
  • description: Methylohalobius crimeensis partial 16S rRNA gene, strain 10Ki
  • accession: AJ581837
  • length: 1462
  • database: ena
  • NCBI tax ID: 1283300

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Methylohalobius crimeensis 10KiGCA_000421465contigncbi1283300
66792Methylohalobius crimeensis 10Ki1283300.3wgspatric1283300
66792Methylohalobius crimeensis 10Ki2524614657draftimg1283300

GC content

  • @ref: 6149
  • GC-content: 58.7

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
motileyes88.193yes
gram-positiveno98.101no
anaerobicno92.994no
aerobicno50no
halophileno70.846yes
spore-formingno78.135yes
thermophileno88.343no
glucose-utilyes68.298no
flagellatedno69.132no
glucose-fermentno85.165no

External links

@ref: 6149

culture collection no.: DSM 16011, ATCC BAA 967

straininfo link

  • @ref: 76658
  • straininfo: 398432

literature

  • topic: Phylogeny
  • Pubmed-ID: 16166672
  • title: Methylohalobius crimeensis gen. nov., sp. nov., a moderately halophilic, methanotrophic bacterium isolated from hypersaline lakes of Crimea.
  • authors: Heyer J, Berger U, Hardt M, Dunfield PF
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijs.0.63213-0
  • year: 2005
  • mesh: Bacterial Typing Techniques, Culture Media, DNA, Bacterial/analysis, Fatty Acids/analysis, Fresh Water/*microbiology, Genes, rRNA, Methane/*metabolism, Methylococcaceae/*classification/genetics/growth & development/isolation & purification, Molecular Sequence Data, Phylogeny, RNA, Ribosomal, 16S, *Sodium Chloride, Ukraine
  • topic2: Cultivation

Reference

@idauthorscataloguedoi/urltitleID_cross_referencepubmed
6149Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 16011)https://www.dsmz.de/collection/catalogue/details/culture/DSM-16011
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
31286Barberan A, Caceres Velazquez H, Jones S, Fierer N.10.1128/mSphere.00237-17Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information2760528776041
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
76658Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID398432.1StrainInfo: A central database for resolving microbial strain identifiers