Methanogenium frigidum (DSM 16458, OCM 469, SMCC 459W)

Name: Methanogenium frigidum (DSM 16458, OCM 469, SMCC 459W)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 7037

Type strain

Strain Designation Ace-2

Culture col. no. DSM 16458 OCM 469 SMCC 459W Ace-2

NCBI tax ID(s) 313587 62970

Special Collections DSMZ

Links

Methanogenium frigidum Ace-2 is an anaerobe, psychrophilic prokaryote that was isolated from water column at a depth of 24m.

anaerobe psychrophilic 16S sequence

Name and taxonomic classification

SI-ID 131977

DSM 16458

@ref 20215

Last LPSN update 2025-12-16 09:48:28

Domain Methanobacteriati

Phylum Methanobacteriota

Class "Methanomicrobia"

Order Methanomicrobiales

Family Methanomicrobiaceae

Genus Methanogenium

Species Methanogenium frigidum

Full scientific name Methanogenium frigidum Franzmann et al. 1997

Morphology

Colony morphology

6449

Incubation period>14 days

Culture and growth conditions

Culture medium

@ref	Name	Growth	Medium link	Composition
6449	METHANOGENIUM MEDIUM (H2/CO2) (DSMZ Medium 141)		Medium recipe at MediaDive	Name: METHANOGENIUM MEDIUM (H2/CO2) (DSMZ Medium 141; with strain-specific modifications) Composition: NaHCO3 4.89237 g/l MgCl2 x 6 H2O 3.91389 g/l Trypticase peptone 1.95695 g/l Yeast extract 1.95695 g/l Na-acetate 0.978474 g/l 2-Mercaptoethanesulfonic acid 0.489237 g/l L-Cysteine HCl x H2O 0.489237 g/l Na2S x 9 H2O 0.489237 g/l KCl 0.332681 g/l NH4Cl 0.244618 g/l K2HPO4 0.136986 g/l MgSO4 x 7 H2O 0.0293542 g/l Nitrilotriacetic acid 0.0146771 g/l NaCl 0.00978474 g/l MnSO4 x H2O 0.00489237 g/l Fe(NH4)2(SO4)2 x 6 H2O 0.00195695 g/l CoSO4 x 7 H2O 0.00176125 g/l ZnSO4 x 7 H2O 0.00176125 g/l CaCl2 x 2 H2O 0.000978474 g/l FeSO4 x 7 H2O 0.000978474 g/l Sodium resazurin 0.000489237 g/l NiCl2 x 6 H2O 0.000293542 g/l AlK(SO4)2 x 12 H2O 0.000195695 g/l CuSO4 x 5 H2O 9.78474e-05 g/l Pyridoxine hydrochloride 9.78474e-05 g/l Na2MoO4 x 2 H2O 9.78474e-05 g/l H3BO3 9.78474e-05 g/l p-Aminobenzoic acid 4.89237e-05 g/l Calcium D-(+)-pantothenate 4.89237e-05 g/l (DL)-alpha-Lipoic acid 4.89237e-05 g/l Nicotinic acid 4.89237e-05 g/l Riboflavin 4.89237e-05 g/l Thiamine HCl 4.89237e-05 g/l Folic acid 1.95695e-05 g/l Biotin 1.95695e-05 g/l Na2WO4 x 2 H2O 3.91389e-06 g/l Na2SeO3 x 5 H2O 2.93542e-06 g/l Vitamin B12 9.78474e-07 g/l Distilled water

Culture temp

@ref	Growth	Type	Temperature (°C)	Range
6449	positive	growth	15	psychrophilic

Physiology and metabolism

Oxygen tolerance

6449

Oxygen toleranceanaerobe

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2
#Environmental	#Aquatic

Isolation

@ref	Sample type	Geographic location	Continent
6449	water column at a depth of 24m	Ace Lake	Australia and Oceania

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
6449	1	Risk group (German classification) According to TRBA 466

Sequence information

Genome sequences

@ref	Description	Assembly level	IMG accession	NCBI tax ID
66792	Methanogenium frigidum Ace-2	complete	2739367766	313587

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
20218	Methanogenium frigidum 16S ribosomal RNA gene, partial sequence	AF009219	732		62970
6449	Methanogenium frigidum partial 16S rRNA gene, type strain DSM 16458T	FR749908	1460		62970

Genome-based predictions

Literature

Title	Authors	Journal	DOI	Year
Biological Contamination Prevention for Outer Solar System Moons of Astrobiological Interest: What Do We Need to Know?	Rettberg P, Antunes A, Brucato J, Cabezas P, Collins G, Haddaji A, Kminek G, Leuko S, McKenna-Lawlor S, Moissl-Eichinger C, Fellous JL, Olsson-Francis K, Pearce D, Rabbow E, Royle S, Saunders M, Sephton M, Spry A, Walter N, Wimmer Schweingruber R, Treuet JC.	Astrobiology	10.1089/ast.2018.1996	2019
Specific bacterial, archaeal, and eukaryotic communities in tidal-flat sediments along a vertical profile of several meters.	Wilms R, Sass H, Kopke B, Koster J, Cypionka H, Engelen B.	Appl Environ Microbiol	10.1128/aem.72.4.2756-2764.2006	2006
Assessing the Ecophysiology of Methanogens in the Context of Recent Astrobiological and Planetological Studies.	Taubner RS, Schleper C, Firneis MG, Rittmann SK.	Life (Basel)	10.3390/life5041652	2015

References

#6449	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 16458
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#20218	Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )
#66792	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Methanogenium frigidum (DSM 16458, OCM 469, SMCC 459W)

Name and taxonomic classification

Morphology

Colony morphology

Culture and growth conditions

Culture medium

Culture temp

Physiology and metabolism

Oxygen tolerance

Isolation, sampling and environmental information

Isolation source categories

Isolation

Interaction and safety

Risk assessment

Sequence information

Genome sequences

16S sequences

Genome-based predictions

Literature

Literature

References

BacDive

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