Strain identifier
BacDive ID: 7037
Type strain: 
Species: Methanogenium frigidum
Strain Designation: Ace-2
Strain history: <- D. R. Boone, Portland State Univ., USA; Ace-2 <- P. D. Franzmann and Y. Liu, University of Tasmania, Hobart, Australia {1995}
NCBI tax ID(s): 313587 (strain), 62970 (species)
version 8.1 (current version)
General
@ref: 6449
BacDive-ID: 7037
DSM-Number: 16458
keywords: genome sequence, 16S sequence, Archaea, anaerobe, psychrophilic
description: Methanogenium frigidum Ace-2 is an anaerobe, psychrophilic archaeon that was isolated from water column at a depth of 24m.
NCBI tax id
| NCBI tax id | Matching level |
|---|---|
| 62970 | species |
| 313587 | strain |
strain history
- @ref: 6449
- history: <- D. R. Boone, Portland State Univ., USA; Ace-2 <- P. D. Franzmann and Y. Liu, University of Tasmania, Hobart, Australia {1995}
doi: 10.13145/bacdive7037.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/archaea
- keyword: phylum/methanobacteriota
- domain: Archaea
- phylum: Methanobacteriota
- class: Methanomicrobia
- order: Methanomicrobiales
- family: Methanomicrobiaceae
- genus: Methanogenium
- species: Methanogenium frigidum
- full scientific name: Methanogenium frigidum Franzmann et al. 1997
@ref: 6449
domain: Archaea
phylum: Euryarchaeota
class: Methanomicrobia
order: Methanomicrobiales
family: Methanomicrobiaceae
genus: Methanogenium
species: Methanogenium frigidum
full scientific name: Methanogenium frigidum Franzmann et al. 1997
strain designation: Ace-2
type strain: yes
Morphology
colony morphology
- @ref: 6449
- incubation period: >14 days
Culture and growth conditions
culture medium
- @ref: 6449
- name: METHANOGENIUM MEDIUM (H2/CO2) (DSMZ Medium 141)
- growth: yes
- link: https://mediadive.dsmz.de/medium/141
- composition: Name: METHANOGENIUM MEDIUM (H2/CO2) (DSMZ Medium 141; with strain-specific modifications) Composition: NaHCO3 4.89237 g/l MgCl2 x 6 H2O 3.91389 g/l Trypticase peptone 1.95695 g/l Yeast extract 1.95695 g/l Na-acetate 0.978474 g/l 2-Mercaptoethanesulfonic acid 0.489237 g/l L-Cysteine HCl x H2O 0.489237 g/l Na2S x 9 H2O 0.489237 g/l KCl 0.332681 g/l NH4Cl 0.244618 g/l K2HPO4 0.136986 g/l MgSO4 x 7 H2O 0.0293542 g/l Nitrilotriacetic acid 0.0146771 g/l NaCl 0.00978474 g/l MnSO4 x H2O 0.00489237 g/l Fe(NH4)2(SO4)2 x 6 H2O 0.00195695 g/l CoSO4 x 7 H2O 0.00176125 g/l ZnSO4 x 7 H2O 0.00176125 g/l CaCl2 x 2 H2O 0.000978474 g/l FeSO4 x 7 H2O 0.000978474 g/l Sodium resazurin 0.000489237 g/l NiCl2 x 6 H2O 0.000293542 g/l AlK(SO4)2 x 12 H2O 0.000195695 g/l CuSO4 x 5 H2O 9.78474e-05 g/l Pyridoxine hydrochloride 9.78474e-05 g/l Na2MoO4 x 2 H2O 9.78474e-05 g/l H3BO3 9.78474e-05 g/l p-Aminobenzoic acid 4.89237e-05 g/l Calcium D-(+)-pantothenate 4.89237e-05 g/l (DL)-alpha-Lipoic acid 4.89237e-05 g/l Nicotinic acid 4.89237e-05 g/l Riboflavin 4.89237e-05 g/l Thiamine HCl 4.89237e-05 g/l Folic acid 1.95695e-05 g/l Biotin 1.95695e-05 g/l Na2WO4 x 2 H2O 3.91389e-06 g/l Na2SeO3 x 5 H2O 2.93542e-06 g/l Vitamin B12 9.78474e-07 g/l Distilled water
culture temp
- @ref: 6449
- growth: positive
- type: growth
- temperature: 15
- range: psychrophilic
Physiology and metabolism
oxygen tolerance
- @ref: 6449
- oxygen tolerance: anaerobe
Isolation, sampling and environmental information
isolation
- @ref: 6449
- sample type: water column at a depth of 24m
- geographic location: Ace Lake
- continent: Australia and Oceania
isolation source categories
- Cat1: #Environmental
- Cat2: #Aquatic
Safety information
risk assessment
- @ref: 6449
- biosafety level: 1
- biosafety level comment: Risk group (German classification)
Sequence information
16S sequences
| @ref | description | accession | length | database | NCBI tax ID |
|---|---|---|---|---|---|
| 20218 | Methanogenium frigidum 16S ribosomal RNA gene, partial sequence | AF009219 | 732 | ena | 62970 |
| 6449 | Methanogenium frigidum partial 16S rRNA gene, type strain DSM 16458T | FR749908 | 1460 | ena | 62970 |
Genome sequences
- @ref: 66792
- description: Methanogenium frigidum Ace-2
- accession: 2739367766
- assembly level: draft
- database: img
- NCBI tax ID: 313587
External links
@ref: 6449
culture collection no.: DSM 16458, OCM 469, SMCC 459W
straininfo link
- @ref: 76497
- straininfo: 131977
Reference
| @id | authors | catalogue | doi/url | title | journal | pubmed |
|---|---|---|---|---|---|---|
| 6449 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 16458) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-16458 | |||
| 20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |||
| 20218 | Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P. | 10.1016/j.syapm.2013.11.002 | StrainInfo introduces electronic passports for microorganisms. | Syst Appl Microbiol. 37: 42-50 2014 | 24321274 | |
| 66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |||
| 76497 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID131977.1 | StrainInfo: A central database for resolving microbial strain identifiers |