Methanogenium marinum (DSM 15558, OCM 752, AK-1)

Name: Methanogenium marinum (DSM 15558, OCM 752, AK-1)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 7036

Type strain

Strain Designation AK-1

Culture col. no. DSM 15558 OCM 752 AK-1

NCBI tax ID(s) 348610

Special Collections DSMZ

History DSMZ <- D. R. Boone, Portland State Univ., USA; AK-1 <- Y. Liu; {1998}

Links

Methanogenium marinum AK-1 is an anaerobe archaeon that was isolated from marine sediments, 38-45 m below the surface.

anaerobe genome sequence 16S sequence Archaea

Name and taxonomic classification

SI-ID 113600

DSM 15558

@ref 20215

Last LPSN update 2026-05-29 08:26:33

Domain Archaea

Phylum Methanobacteriota

Class "Methanomicrobia"

Order Methanomicrobiales

Family Methanomicrobiaceae

Genus Methanogenium

Species Methanogenium marinum

Full scientific name Methanogenium marinum Chong et al. 2003

Morphology

Colony morphology

6001

Incubation period3-7 days

Culture and growth conditions

Culture medium

@ref	Name	Growth	Medium link	Composition
6001	METHANOGENIUM MEDIUM (H2/CO2) (DSMZ Medium 141)		Medium recipe at MediaDive	Name: METHANOGENIUM MEDIUM (H2/CO2) (DSMZ Medium 141; with strain-specific modifications) Composition: NaHCO3 4.89237 g/l MgCl2 x 6 H2O 3.91389 g/l Trypticase peptone 1.95695 g/l Yeast extract 1.95695 g/l Na-acetate 0.978474 g/l 2-Mercaptoethanesulfonic acid 0.489237 g/l L-Cysteine HCl x H2O 0.489237 g/l Na2S x 9 H2O 0.489237 g/l KCl 0.332681 g/l NH4Cl 0.244618 g/l K2HPO4 0.136986 g/l MgSO4 x 7 H2O 0.0293542 g/l Nitrilotriacetic acid 0.0146771 g/l NaCl 0.00978474 g/l MnSO4 x H2O 0.00489237 g/l Fe(NH4)2(SO4)2 x 6 H2O 0.00195695 g/l CoSO4 x 7 H2O 0.00176125 g/l ZnSO4 x 7 H2O 0.00176125 g/l CaCl2 x 2 H2O 0.000978474 g/l FeSO4 x 7 H2O 0.000978474 g/l Sodium resazurin 0.000489237 g/l NiCl2 x 6 H2O 0.000293542 g/l AlK(SO4)2 x 12 H2O 0.000195695 g/l CuSO4 x 5 H2O 9.78474e-05 g/l Pyridoxine hydrochloride 9.78474e-05 g/l Na2MoO4 x 2 H2O 9.78474e-05 g/l H3BO3 9.78474e-05 g/l p-Aminobenzoic acid 4.89237e-05 g/l Calcium D-(+)-pantothenate 4.89237e-05 g/l (DL)-alpha-Lipoic acid 4.89237e-05 g/l Nicotinic acid 4.89237e-05 g/l Riboflavin 4.89237e-05 g/l Thiamine HCl 4.89237e-05 g/l Folic acid 1.95695e-05 g/l Biotin 1.95695e-05 g/l Na2WO4 x 2 H2O 3.91389e-06 g/l Na2SeO3 x 5 H2O 2.93542e-06 g/l Vitamin B12 9.78474e-07 g/l Distilled water

Culture temp

@ref	Growth	Type	Temperature (°C)
6001	positive	growth	22

Physiology and metabolism

Oxygen tolerance

6001

Oxygen toleranceanaerobe

Spore formation

@ref	Spore formation	Confidence
125438		98.62

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Environmental	#Terrestrial	#Sediment

Isolation

@ref	Sample type	Geographic location	Country	Country ISO 3 Code	Continent	Latitude	Longitude
6001	marine sediments, 38-45 m below the surface	Alaska, Aleuten Islands, Unalaska Island, Skan Bay	USA	USA	North America	53.6167	-167.05 53.6167/-167.05

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
6001	1	Risk group (German classification) According to TRBA 466

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	IMG accession	NCBI tax ID	Score
66792	ASM2891240v1 assembly for Methanogenium marinum DSM 15558	contig	GCA_028912405	8064710606	348610	59.2

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
6001	Methanogenium marinum strain AK-1 16S ribosomal RNA gene, partial sequence	DQ177344	1436		348610

Genome-based predictions

Predictions

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Methanogenium marinum DSM 15558
NCBI: GCA_028912405.1

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	no	68.70	no
125438	anaerobic	anaerobicⓘ	yes	66.38	yes
125438	aerobic	aerobicⓘ	no	85.30	yes
125438	spore-forming	spore-formingⓘ	no	98.62	no
125438	thermophilic	thermophileⓘ	no	84.63	no
125438	flagellated	motile2+ⓘ	no	78.35	no

Literature

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Isolation and characterization of a new Methanoculleus bourgensis strain KOR-2 from the rumen of Holstein steers.	Battumur U, Lee M, Bae GS, Kim CH.	Asian-Australas J Anim Sci	10.5713/ajas.18.0409	2019
	The Historical Development of Cultivation Techniques for Methanogens and Other Strict Anaerobes and Their Application in Modern Microbiology.	Hanisakova N, Vitezova M, Rittmann SKR.	Microorganisms	10.3390/microorganisms10020412	2022
Biotechnology	Microbial Diversity in Sulfate-Reducing Marine Sediment Enrichment Cultures Associated with Anaerobic Biotransformation of Coastal Stockpiled Phosphogypsum (Sfax, Tunisia).	Zouch H, Karray F, Armougom F, Chifflet S, Hirschler-Rea A, Kharrat H, Kamoun L, Ben Hania W, Ollivier B, Sayadi S, Quemeneur M.	Front Microbiol	10.3389/fmicb.2017.01583	2017
Phylogeny	Diversity of Archaea in marine sediments from Skan Bay, Alaska, including cultivated methanogens, and description of Methanogenium boonei sp. nov.	Kendall MM, Wardlaw GD, Tang CF, Bonin AS, Liu Y, Valentine DL.	Appl Environ Microbiol	10.1128/aem.01154-06	2007
Phylogeny	Isolation of a methanogen from deep marine sediments that contain methane hydrates, and description of Methanoculleus submarinus sp. nov.	Mikucki JA, Liu Y, Delwiche M, Colwell FS, Boone DR.	Appl Environ Microbiol	10.1128/aem.69.6.3311-3316.2003	2003
Phylogeny	Methanogenium marinum sp. nov., a H2-using methanogen from Skan Bay, Alaska, and kinetics of H2 utilization.	Chong SC, Liu Y, Cummins M, Valentine DL, Boone DR	Antonie Van Leeuwenhoek	10.1023/a:1020535222281	2002

References

#6001	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 15558
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#66792	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

Content

About us

Methanogenium marinum (DSM 15558, OCM 752, AK-1)

Name and taxonomic classification

Morphology

Colony morphology

Culture and growth conditions

Culture medium

Culture temp

Physiology and metabolism

Oxygen tolerance

Spore formation

Isolation, sampling and environmental information

Isolation source categories

Isolation

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_028912405

16S sequences

Genome-based predictions

Predictions

Literature

Literature

References

BacDive

Help

Social Media