General

@ref: 6001

BacDive-ID: 7036

DSM-Number: 15558

keywords: genome sequence, 16S sequence, Archaea, anaerobe, psychrophilic

description: Methanogenium marinum AK-1 is an anaerobe, psychrophilic archaeon that was isolated from marine sediments, 38-45 m below the surface.

NCBI tax id

• NCBI tax id: 348610
• Matching level: species

strain history

• @ref: 6001
• history: <- D. R. Boone, Portland State Univ., USA; AK-1 <- Y. Liu {1998}

doi: 10.13145/bacdive7036.20230509.8.1

Name and taxonomic classification

LPSN

• @ref: 20215
• description: domain/archaea
• keyword: phylum/methanobacteriota
• domain: Archaea
• phylum: Methanobacteriota
• class: Methanomicrobia
• order: Methanomicrobiales
• family: Methanomicrobiaceae
• genus: Methanogenium
• species: Methanogenium marinum
• full scientific name: Methanogenium marinum Chong et al. 2003

@ref: 6001

domain: Archaea

phylum: Euryarchaeota

class: Methanomicrobia

order: Methanomicrobiales

family: Methanomicrobiaceae

genus: Methanogenium

species: Methanogenium marinum

full scientific name: Methanogenium marinum Chong et al. 2003

strain designation: AK-1

type strain: yes

Morphology

colony morphology

• @ref: 6001
• incubation period: 3-7 days

Culture and growth conditions

culture medium

• @ref: 6001
• name: METHANOGENIUM MEDIUM (H2/CO2) (DSMZ Medium 141)
• growth: yes
• link: https://mediadive.dsmz.de/medium/141
• composition: Name: METHANOGENIUM MEDIUM (H2/CO2) (DSMZ Medium 141; with strain-specific modifications) Composition: NaHCO3 4.89237 g/l MgCl2 x 6 H2O 3.91389 g/l Trypticase peptone 1.95695 g/l Yeast extract 1.95695 g/l Na-acetate 0.978474 g/l 2-Mercaptoethanesulfonic acid 0.489237 g/l L-Cysteine HCl x H2O 0.489237 g/l Na2S x 9 H2O 0.489237 g/l KCl 0.332681 g/l NH4Cl 0.244618 g/l K2HPO4 0.136986 g/l MgSO4 x 7 H2O 0.0293542 g/l Nitrilotriacetic acid 0.0146771 g/l NaCl 0.00978474 g/l MnSO4 x H2O 0.00489237 g/l Fe(NH4)2(SO4)2 x 6 H2O 0.00195695 g/l CoSO4 x 7 H2O 0.00176125 g/l ZnSO4 x 7 H2O 0.00176125 g/l CaCl2 x 2 H2O 0.000978474 g/l FeSO4 x 7 H2O 0.000978474 g/l Sodium resazurin 0.000489237 g/l NiCl2 x 6 H2O 0.000293542 g/l AlK(SO4)2 x 12 H2O 0.000195695 g/l CuSO4 x 5 H2O 9.78474e-05 g/l Pyridoxine hydrochloride 9.78474e-05 g/l Na2MoO4 x 2 H2O 9.78474e-05 g/l H3BO3 9.78474e-05 g/l p-Aminobenzoic acid 4.89237e-05 g/l Calcium D-(+)-pantothenate 4.89237e-05 g/l (DL)-alpha-Lipoic acid 4.89237e-05 g/l Nicotinic acid 4.89237e-05 g/l Riboflavin 4.89237e-05 g/l Thiamine HCl 4.89237e-05 g/l Folic acid 1.95695e-05 g/l Biotin 1.95695e-05 g/l Na2WO4 x 2 H2O 3.91389e-06 g/l Na2SeO3 x 5 H2O 2.93542e-06 g/l Vitamin B12 9.78474e-07 g/l Distilled water

culture temp

• @ref: 6001
• growth: positive
• type: growth
• temperature: 22
• range: psychrophilic

Physiology and metabolism

oxygen tolerance

• @ref: 6001
• oxygen tolerance: anaerobe

Isolation, sampling and environmental information

isolation

• @ref: 6001
• sample type: marine sediments, 38-45 m below the surface
• geographic location: Alaska, Aleuten Islands, Unalaska Island, Skan Bay
• country: USA
• origin.country: USA
• continent: North America
• latitude: 53.6167
• longitude: -167.05

isolation source categories

• Cat1: #Environmental
• Cat2: #Terrestrial
• Cat3: #Sediment

Safety information

risk assessment

• @ref: 6001
• biosafety level: 1
• biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

• @ref: 6001
• description: Methanogenium marinum strain AK-1 16S ribosomal RNA gene, partial sequence
• accession: DQ177344
• length: 1436
• database: ena
• NCBI tax ID: 348610

Genome sequences

• @ref: 66792
• description: Methanogenium marinum DSM 15558
• accession: GCA_028912405
• assembly level: contig
• database: ncbi
• NCBI tax ID: 348610

Genome-based predictions

predictions

External links

@ref: 6001

culture collection no.: DSM 15558, OCM 752

straininfo link

• @ref: 76496
• straininfo: 113600

literature

• topic: Phylogeny
• Pubmed-ID: 12448725
• title: Methanogenium marinum sp. nov., a H2-using methanogen from Skan Bay, Alaska, and kinetics of H2 utilization.
• authors: Chong SC, Liu Y, Cummins M, Valentine DL, Boone DR
• journal: Antonie Van Leeuwenhoek
• DOI: 10.1023/a:1020535222281
• year: 2002
• mesh: Alaska, Bacterial Typing Techniques, Cold Temperature, Culture Media, DNA, Ribosomal/analysis, Geologic Sediments/microbiology, Hydrogen/*metabolism, Kinetics, Methane/*metabolism, Methanomicrobiaceae/*classification/genetics/growth & development/*isolation & purification, Nucleic Acid Hybridization, RNA, Ribosomal, 16S/genetics, Seawater/*microbiology
• topic2: Enzymology

Reference