Name: Methanothermobacter wolfei DSM 2970
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 6894

Type strain:

Species: Methanothermobacter wolfei

Culture col. no.: DSM 2970, ATCC 43096, NBRC 100332, VKM B-1829, JCM 14652, OCM 154

Strain history: NBRC 100332 <-- DSM 2970 <-- J. Winter.

NCBI tax ID(s): 145261 (species)

SI-ID 41645

ATCC 43096, VKM B-1829, NBRC 100332, JCM 14652, DSM 2970

Section

Methanothermobacter wolfei DSM 2970 is an anaerobe, thermophilic archaeon that was isolated from mixture of sewage sludge and river sediment.

anaerobe
thermophilic
16S sequence
Archaea
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Archaea"
	Phylum	*Methanobacteriota*
	Class	*Methanobacteria*
	Order	*Methanobacteriales*
	Family	*Methanobacteriaceae*
	Genus	*Methanothermobacter*
	Species	**Methanothermobacter wolfei**
	Full Scientific Name (LPSN)	Methanothermobacter wolfei corrig. (Winter et al. 1985) Wasserfallen et al. 2000

	Synonym	Methanothermobacter wolfeii
	Synonym	Methanobacterium wolfei

Information on culture and growth conditions Culture and growth conditions

[Ref.: #1264]

Culture medium

METHANOBACTERIUM MEDIUM (DSMZ Medium 119)

[Ref.: #1264]

Culture medium growth

[Ref.: #1264]

Culture medium link

Medium recipe at MediaDive

[Ref.: #1264]

Culture medium composition

expand / minimize

	Temperatures	Kind of temperature		Temperature
[Ref.: #1264]			growth	60 °C
[Ref.: #67770]			growth	60 °C

[Ref.: #1264]	Temperature range	thermophilic
[Ref.: #67770]	Temperature range	thermophilic

Information on physiology and metabolism Physiology and metabolism

[Ref.: #1264]

Oxygen tolerance

anaerobe

[Ref.: #1264]	Name of produced compound	autolytic enzymes
[Ref.: #1264]	Name of produced compound	restriction endonuclease MwoI

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #1264]	Sample type/isolated from	mixture of sewage sludge and river sediment
[Ref.: #1264]	Country	Germany
[Ref.: #1264]	Country ISO 3 Code	DEU
[Ref.: #1264]	Continent	Europe

[Ref.: #67770]	Sample type/isolated from	Mixture of sewage sludge and river sediment
* marker position based on {}

Isolation sources categories

#Engineered	#Waste	#Sewage sludge
#Environmental	#Aquatic	#River (Creek)
#Environmental	#Aquatic	#Sediment

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence AB104858 (>99% sequence identity) for Methanothermobacter from Microbeatlas

Aquatic Samples	38
Soil Samples	18
Animal Samples	3
Plant Samples	5
Total Samples	64

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #1264]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #1264]	Methanothermobacter wolfeii gene for 16S rRNA, partial sequence	AB104858	1446	ENA	145261 tax ID
[Ref.: #20218]	M.wolfei gene for 16S ribosomal RNA	X89406	971	ENA	145261 tax ID	*

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Methanothermobacter wolfeii DSM 2970	GCA_025397995	complete	GenBank	145261 tax ID	*

[Ref.: #1264]	GC-content	61.0 mol%
[Ref.: #67770]	GC-content	48.5 mol%	thermal denaturation, midpoint method (Tm)
[Ref.: #67770]	GC-content	61 mol%	thermal denaturation, midpoint method (Tm)

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Methanothermobacter wolfeii DSM 2970 NCBI: GCA_025397995.1
[Ref.: #69480]	motile	no	85.616	no
[Ref.: #69480]	flagellated	no	96.142	no
[Ref.: #69480]	gram-positive	no	97.498	no
[Ref.: #69480]	anaerobic	yes	98.874	yes
[Ref.: #69480]	aerobic	no	97.078	yes
[Ref.: #69480]	halophile	no	79.792	no
[Ref.: #69480]	spore-forming	no	96.742	no
[Ref.: #69480]	glucose-ferment	no	59.435	no
[Ref.: #69480]	thermophile	yes	93.62	no
[Ref.: #69480]	glucose-util	yes	74.349	no

Availability in culture collections External links

[Ref.: #1264]

Culture collection no.

DSM 2970, ATCC 43096, NBRC 100332, VKM B-1829, JCM 14652, OCM 154

[Ref.: #76358]

SI-ID 41645

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Isolation and characterization of Methanothermobacter crinale sp. nov., a novel hydrogenotrophic methanogen from the Shengli oil field.	Cheng L, Dai L, Li X, Zhang H, Lu Y	Appl Environ Microbiol	10.1128/AEM.00210-11	2011	*
Phylogeny	Phylogenetic analysis of 18 thermophilic Methanobacterium isolates supports the proposals to create a new genus, Methanothermobacter gen. nov., and to reclassify several isolates in three species, Methanothermobacter thermautotrophicus comb. nov., Methanothermobacter wolfeii comb. nov., and Methanothermobacter marburgensis sp. nov.	Wasserfallen A, Nolling J, Pfister P, Reeve J, Conway de Macario E	Int J Syst Evol Microbiol	10.1099/00207713-50-1-43	2000	*

References

#1264

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 2970

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#20218

Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#76358

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID41645.1 )

* These data were automatically processed and therefore are not curated

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Methanothermobacter wolfeii DSM 2970

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