Methanothermobacter wolfei | Type strain | DSM 2970, ATCC 43096, NBRC 100332, VKM B-1829

Strain identifier

BacDive ID: 6894

Type strain:

Species: Methanothermobacter wolfei

Culture col. no.: DSM 2970, ATCC 43096, NBRC 100332, VKM B-1829

Section

Name and taxonomic classification
Morphology and physiology
Culture and growth conditions
Isolation, sampling and environmental information
Application and interaction
Molecular biology
Strain availability

External links

Methanothermobacter wolfei DSM 2970 is an anaerobe, thermophilic archaeon that was isolated from mixture of sewage sludge and river sediment.

anaerobe
thermophilic
16S sequence
Archaea
genome sequence

Information on the name and the taxonomic classification.Name and taxonomic classification

	Last LPSN update	10-09-2020 (DD-MM-YYYY)
	Domain	Archaea
	Phylum	Euryarchaeota
	Class	Methanobacteria
	Order	Methanobacteriales
	Family	Methanobacteriaceae
	Genus	Methanothermobacter
	Species	Methanothermobacter wolfei
	Full Scientific Name (PNU)	Methanothermobacter wolfei corrig. (Winter et al. 1985) Wasserfallen et al. 2000

	Synonym	Methanothermobacter wolfeii
	Synonym	Methanobacterium wolfei

Information on morphological and physiological propertiesMorphology and physiology

[Ref.: #1264]	Name of produced compound	autolytic enzymes
[Ref.: #1264]	Name of produced compound	restriction endonuclease MwoI

[Ref.: #1264]

Oxygen tolerance

anaerobe

Information on culture and growth conditionsCulture and growth conditions

[Ref.: #1264]

Culture medium

METHANOBACTERIUM MEDIUM (DSMZ Medium 119), 60°C, anaerobic

[Ref.: #1264]

Culture medium growth

[Ref.: #1264]

Culture medium link

medium recipe provided by DSMZ

	Temperatures	Kind of temperature		Temperature
[Ref.: #1264]			growth	60 ̊C

[Ref.: #1264]

Temperature range

thermophilic

Information on isolation source, the sampling and environmental conditionsIsolation, sampling and environmental information

[Ref.: #1264]

Sample type/isolated from

mixture of sewage sludge and river sediment

[Ref.: #1264]

Country

Germany

[Ref.: #1264]

Country ISO 3 Code

DEU

[Ref.: #1264]

Continent

Europe

Isolation sources categories

#Engineered	#Waste	#Sewage sludge
#Environmental	#Aquatic	#River (Creek)
#Environmental	#Aquatic	#Sediment

What are isolation sources categories?

* marker position based on {}

Information on possible application of the strain and its possible interaction with e.g. potential hostsApplication and interaction

[Ref.: #1264]

Biosafety level

Risk group (German classification)

Information on genomic background e.g. entries in nucleic sequence databassMolecular biology

[Ref.: #1264]

GC-content

61.0 mol%

Sequence information:

Only first 5 entries are displayed. Click here to see all.

	Sequence database	Sequence accession description	Sequence accession number	Sequence type	Sequence length (bp)	Associated NCBI tax ID
[Ref.: #1264]	16S rRNA gene, Marker Gene (DDBJ Direct submission)	Methanothermobacter wolfeii gene for 16S rRNA, partial sequence	AB104858 ENA	16S	1446	145261 tax ID
[Ref.: #20218]	Marker Gene (DDBJ Direct submission)	Methanothermobacter wolfeii mcrA gene for methyl-coenzyme M reductase alpha subunit, partial cds	AB300780 ENA		719	145261 tax ID	*
[Ref.: #20218]	Marker Gene (GenBank Direct submission)	Methanothermobacter wolfeii strain DSM 2970 methyl-coenzyme M reductase I alpha subunit (mcrA) gene, partial cds	AY289748 ENA		440	145261 tax ID	*
[Ref.: #20218]	Marker Gene (GenBank Direct submission)	Methanothermobacter wolfeii strain DSM 2970 methyl-coenzyme M reductase II alpha subunit (mrtA) gene, partial cds	AY289749 ENA		440	145261 tax ID	*
[Ref.: #20218]	Marker Gene (EMBL Direct submission)	M.wolfei gene for 16S ribosomal RNA	X89406 ENA	16S	971	145261 tax ID	*
[Ref.: #20218]	Marker Gene (DDBJ Direct submission)	Methanothermobacter wolfeii mcrA gene for methyl-coenzyme M reductase I alpha subunit, partial cds, strain: NBRC 100332	AB703638 ENA		1147	145261 tax ID	*
[Ref.: #20218]	Marker Gene (DDBJ Direct submission)	Methanothermobacter wolfeii mrtA gene for methyl-coenzyme M reductase II alpha subunit, partial cds, strain: NBRC 100332	AB703639 ENA		1144	145261 tax ID	*

Availability in culture collectionsStrain availability

[Ref.: #1264]

Culture collection no.

DSM 2970, ATCC 43096, NBRC 100332, VKM B-1829

[Ref.: #1264]

Strain history

<- J. Winter, Univ. Regensburg, Germany

[Ref.: #20218]

Associated Passport(s) in StrainInfo

112129, 112128, 401416, 158247

References

#1264

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 2970

#20215

Leibniz Institute DSMZ D.Gleim, M.Kracht, N.Weiss et. al.: Prokaryotic Nomenclature Up-to-date - compilation of all names of Bacteria and Archaea, validly published according to the Bacteriological Code since 1. Jan. 1980, and validly published nomenclatural changes since .

#20218

Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )

* These References are textmined

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