Strain identifier
BacDive ID: 6894
Type strain: 
Species: Methanothermobacter wolfei
Strain history: NBRC 100332 <-- DSM 2970 <-- J. Winter.
NCBI tax ID(s): 145261 (species)
version 8.1 (current version)
General
@ref: 1264
BacDive-ID: 6894
DSM-Number: 2970
keywords: genome sequence, 16S sequence, Archaea, anaerobe, thermophilic
description: Methanothermobacter wolfei DSM 2970 is an anaerobe, thermophilic archaeon that was isolated from mixture of sewage sludge and river sediment.
NCBI tax id
- NCBI tax id: 145261
- Matching level: species
strain history
| @ref | history |
|---|---|
| 1264 | <- J. Winter, Univ. Regensburg, Germany |
| 67770 | NBRC 100332 <-- DSM 2970 <-- J. Winter. |
doi: 10.13145/bacdive6894.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/archaea
- keyword: phylum/methanobacteriota
- domain: Archaea
- phylum: Methanobacteriota
- class: Methanobacteria
- order: Methanobacteriales
- family: Methanobacteriaceae
- genus: Methanothermobacter
- species: Methanothermobacter wolfei
- full scientific name: Methanothermobacter wolfei corrig. (Winter et al. 1985) Wasserfallen et al. 2000
synonyms
@ref synonym 20215 Methanothermobacter wolfeii 20215 Methanobacterium wolfei
@ref: 1264
domain: Archaea
phylum: Euryarchaeota
class: Methanobacteria
order: Methanobacteriales
family: Methanobacteriaceae
genus: Methanothermobacter
species: Methanothermobacter wolfei
full scientific name: Methanothermobacter wolfei (Winter et al. 1985) Wasserfallen et al. 2000
type strain: yes
Culture and growth conditions
culture medium
- @ref: 1264
- name: METHANOBACTERIUM MEDIUM (DSMZ Medium 119)
- growth: yes
- link: https://mediadive.dsmz.de/medium/119
- composition: Name: METHANOBACTERIUM MEDIUM (DSMZ Medium 119) Composition: NaHCO3 3.98804 g/l Na-formate 1.99402 g/l Na-acetate 0.997009 g/l Na2S x 9 H2O 0.498504 g/l L-Cysteine HCl x H2O 0.498504 g/l KH2PO4 0.498504 g/l NH4Cl 0.398804 g/l NaCl 0.398804 g/l MgSO4 x 7 H2O 0.398804 g/l Yeast extract 0.199402 g/l CaCl2 x 2 H2O 0.0498504 g/l HCl 0.00249252 g/l FeSO4 x 7 H2O 0.00199402 g/l FeCl2 x 4 H2O 0.00149551 g/l Sodium resazurin 0.000498504 g/l CoCl2 x 6 H2O 0.000189432 g/l MnCl2 x 4 H2O 9.97009e-05 g/l ZnCl2 6.97906e-05 g/l Na2MoO4 x 2 H2O 3.58923e-05 g/l NiCl2 x 6 H2O 2.39282e-05 g/l H3BO3 5.98205e-06 g/l CuCl2 x 2 H2O 1.99402e-06 g/l Isobutyric acid DL-2-Methylbutyric acid Valeric acid Isovaleric acid H2SO4 Sludge Distilled water
culture temp
| @ref | growth | type | temperature | range |
|---|---|---|---|---|
| 1264 | positive | growth | 60 | thermophilic |
| 67770 | positive | growth | 60 | thermophilic |
Physiology and metabolism
oxygen tolerance
- @ref: 1264
- oxygen tolerance: anaerobe
compound production
| @ref | compound |
|---|---|
| 1264 | autolytic enzymes |
| 1264 | restriction endonuclease MwoI |
Isolation, sampling and environmental information
isolation
| @ref | sample type | country | origin.country | continent |
|---|---|---|---|---|
| 1264 | mixture of sewage sludge and river sediment | Germany | DEU | Europe |
| 67770 | Mixture of sewage sludge and river sediment |
isolation source categories
| Cat1 | Cat2 | Cat3 |
|---|---|---|
| #Engineered | #Waste | #Sewage sludge |
| #Environmental | #Aquatic | #River (Creek) |
| #Environmental | #Aquatic | #Sediment |
taxonmaps
- @ref: 69479
- File name: preview.99_187374.png
- url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_17808;96_68809;97_89451;98_121724;99_187374&stattab=map
- Last taxonomy: Methanothermobacter
- 16S sequence: AB104858
- Sequence Identity:
- Total samples: 64
- soil counts: 18
- aquatic counts: 38
- animal counts: 3
- plant counts: 5
Safety information
risk assessment
- @ref: 1264
- biosafety level: 1
- biosafety level comment: Risk group (German classification)
Sequence information
16S sequences
| @ref | description | accession | length | database | NCBI tax ID |
|---|---|---|---|---|---|
| 20218 | M.wolfei gene for 16S ribosomal RNA | X89406 | 971 | ena | 145261 |
| 1264 | Methanothermobacter wolfeii gene for 16S rRNA, partial sequence | AB104858 | 1446 | ena | 145261 |
Genome sequences
- @ref: 66792
- description: Methanothermobacter wolfeii DSM 2970
- accession: GCA_025397995
- assembly level: complete
- database: ncbi
- NCBI tax ID: 145261
GC content
| @ref | GC-content | method |
|---|---|---|
| 1264 | 61.0 | |
| 67770 | 48.5 | thermal denaturation, midpoint method (Tm) |
| 67770 | 61 | thermal denaturation, midpoint method (Tm) |
Genome-based predictions
predictions
| trait | prediction | confidence | training_data |
|---|---|---|---|
| motile | no | 85.616 | no |
| flagellated | no | 96.142 | no |
| gram-positive | no | 97.498 | no |
| anaerobic | yes | 98.874 | yes |
| aerobic | no | 97.078 | yes |
| halophile | no | 79.792 | no |
| spore-forming | no | 96.742 | no |
| glucose-ferment | no | 59.435 | no |
| thermophile | yes | 93.62 | no |
| glucose-util | yes | 74.349 | no |
External links
@ref: 1264
culture collection no.: DSM 2970, ATCC 43096, NBRC 100332, VKM B-1829, JCM 14652, OCM 154
straininfo link
- @ref: 76358
- straininfo: 41645
literature
| topic | Pubmed-ID | title | authors | journal | DOI | year | mesh | topic2 |
|---|---|---|---|---|---|---|---|---|
| Phylogeny | 10826786 | Phylogenetic analysis of 18 thermophilic Methanobacterium isolates supports the proposals to create a new genus, Methanothermobacter gen. nov., and to reclassify several isolates in three species, Methanothermobacter thermautotrophicus comb. nov., Methanothermobacter wolfeii comb. nov., and Methanothermobacter marburgensis sp. nov. | Wasserfallen A, Nolling J, Pfister P, Reeve J, Conway de Macario E | Int J Syst Evol Microbiol | 10.1099/00207713-50-1-43 | 2000 | Antigens, Archaeal/classification, Bacteriophage Typing, Base Sequence, DNA, Archaeal/genetics, DNA, Ribosomal/genetics, Methanobacteriaceae/*classification/*genetics/immunology/physiology, Methanobacterium/*classification/*genetics/immunology/physiology, Molecular Sequence Data, Phenotype, Phylogeny, Plasmids, RNA, Ribosomal, 16S/genetics, Sequence Alignment, Sequence Analysis, DNA, Temperature | Genetics |
| Phylogeny | 21705537 | Isolation and characterization of Methanothermobacter crinale sp. nov., a novel hydrogenotrophic methanogen from the Shengli oil field. | Cheng L, Dai L, Li X, Zhang H, Lu Y | Appl Environ Microbiol | 10.1128/AEM.00210-11 | 2011 | Acetates/metabolism, Anaerobiosis, Bacterial Typing Techniques/*methods, Base Composition, Base Sequence, Carbon/metabolism, China, DNA, Bacterial/analysis/chemistry/genetics, DNA, Ribosomal/analysis/genetics, Methane/biosynthesis, Methanobacteriaceae/classification/genetics/growth & development/*isolation & purification, Molecular Sequence Data, Oil and Gas Fields/*microbiology, Oxidation-Reduction, Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Temperature | Metabolism |
Reference
| @id | authors | catalogue | doi/url | title | journal | pubmed |
|---|---|---|---|---|---|---|
| 1264 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 2970) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-2970 | |||
| 20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |||
| 20218 | Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P. | 10.1016/j.syapm.2013.11.002 | StrainInfo introduces electronic passports for microorganisms. | Syst Appl Microbiol. 37: 42-50 2014 | 24321274 | |
| 66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |||
| 67770 | Curators of the JCM | https://jcm.brc.riken.jp/en/ | ||||
| 69479 | João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich. | https://microbeatlas.org/ | MicrobeAtlas 1.0 beta | |||
| 76358 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID41645.1 | StrainInfo: A central database for resolving microbial strain identifiers |