Methanothermobacter thermophilus (DSM 6529, VKM B-1786, M)

Name: Methanothermobacter thermophilus (DSM 6529, VKM B-1786, M)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 6895

Type strain

Strain Designation M

Culture col. no. DSM 6529 VKM B-1786 M

NCBI tax ID(s) 49341

Special Collections DSMZ

History <- VKM <- A.Ya. Obraztsova, M

Links

Methanothermobacter thermophilus M is an anaerobe archaeon that was isolated from thermophilic methane tank.

anaerobe 16S sequence Archaea

Name and taxonomic classification

SI-ID 48230

DSM 6529,VKM B-1786,DSM 6530,ATCC BAA-1076

@ref 20215

Last LPSN update 2026-02-09 11:19:20

Domain Archaea

Phylum Methanobacteriota

Class Methanobacteria

Order Methanobacteriales

Family Methanobacteriaceae

Genus Methanothermobacter

Species Methanothermobacter thermophilus

Full scientific name Methanothermobacter thermophilus (Laurinavichus et al. 1990) Boone 2002

Synonyms (2)

"Methanothermobacter thermophilus"
Methanobacterium thermophilum

Morphology

Culture and growth conditions

Culture medium

@ref	Name	Growth	Medium link	Composition
2716	METHANOBACTERIUM MEDIUM (DSMZ Medium 119)		Medium recipe at MediaDive	Name: METHANOBACTERIUM MEDIUM (DSMZ Medium 119) Composition: NaHCO3 3.98804 g/l Na-formate 1.99402 g/l Na-acetate 0.997009 g/l Na2S x 9 H2O 0.498504 g/l L-Cysteine HCl x H2O 0.498504 g/l KH2PO4 0.498504 g/l NH4Cl 0.398804 g/l NaCl 0.398804 g/l MgSO4 x 7 H2O 0.398804 g/l Yeast extract 0.199402 g/l CaCl2 x 2 H2O 0.0498504 g/l HCl 0.00249252 g/l FeSO4 x 7 H2O 0.00199402 g/l FeCl2 x 4 H2O 0.00149551 g/l Sodium resazurin 0.000498504 g/l CoCl2 x 6 H2O 0.000189432 g/l MnCl2 x 4 H2O 9.97009e-05 g/l ZnCl2 6.97906e-05 g/l Na2MoO4 x 2 H2O 3.58923e-05 g/l NiCl2 x 6 H2O 2.39282e-05 g/l H3BO3 5.98205e-06 g/l CuCl2 x 2 H2O 1.99402e-06 g/l Isobutyric acid DL-2-Methylbutyric acid Valeric acid Isovaleric acid H2SO4 Sludge Distilled water

Culture temp

@ref	Growth	Type	Temperature (°C)
2716	positive	growth	60

Physiology and metabolism

Oxygen tolerance

2716

Oxygen toleranceanaerobe

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Engineered	#Built environment	#Container (Reservoir)
#Condition	#Anoxic (anaerobic)	-
#Condition	#Thermophilic (>45°C)	-

Isolation

@ref	Sample type	Country	Continent
2716	thermophilic methane tank	USSR	Asia

Taxonmaps

69479

Global distribution of 16S sequence X99048 (>99% sequence identity) for Methanothermobacter thermophilus from Microbeatlas

Aquatic Samples	49
Soil Samples
Animal Samples	1
Plant Samples	1
Total Samples	51

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
2716	1	Risk group (German classification) According to TRBA 466

Sequence information

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
20218	M.thermophilum 16S rRNA gene	X99048	1432		49341
2716	Methanothermobacter thermophilus partial 16S rRNA gene, strain DSM 6529 type strain	LT626260	1374		49341

GC content

2716

GC-content (mol%)44.7

Genome-based predictions

Literature

Topic	Title	Authors	Journal	DOI	Year
	Methanogenic performance and microbial community during thermophilic digestion of food waste and sewage sludge in a high-solid anaerobic membrane bioreactor.	Li Y, Ni J, Cheng H, Zhu A, Guo G, Qin Y, Li YY.	Bioresour Technol	10.1016/j.biortech.2021.125938	2021
Metabolism	Hyperthermophilic methanogenic archaea act as high-pressure CH₄ cell factories.	Mauerhofer LM, Zwirtmayr S, Pappenreiter P, Bernacchi S, Seifert AH, Reischl B, Schmider T, Taubner RS, Paulik C, Rittmann SKR.	Commun Biol	10.1038/s42003-021-01828-5	2021
Phylogeny	Phylogenetic comparison of the methanogenic communities from an acidic, oligotrophic fen and an anaerobic digester treating municipal wastewater sludge.	Steinberg LM, Regan JM.	Appl Environ Microbiol	10.1128/aem.00553-08	2008
Phylogeny	Microbial communities from methane hydrate-bearing deep marine sediments in a forearc basin.	Reed DW, Fujita Y, Delwiche ME, Blackwelder DB, Sheridan PP, Uchida T, Colwell FS.	Appl Environ Microbiol	10.1128/aem.68.8.3759-3770.2002	2002

References

#2716	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 6529
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#20218	Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Methanothermobacter thermophilus (DSM 6529, VKM B-1786, M)

Name and taxonomic classification

Morphology

Culture and growth conditions

Culture medium

Culture temp

Physiology and metabolism

Oxygen tolerance

Isolation, sampling and environmental information

Isolation source categories

Isolation

Taxonmaps

Interaction and safety

Risk assessment

Sequence information

16S sequences

GC content

Genome-based predictions

Literature

Literature

References

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