Liquorilactobacillus oeni (DSM 19972, CECT 7334, 59b)

Name: Liquorilactobacillus oeni (DSM 19972, CECT 7334, 59b)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 6676

Type strain

Strain Designation 59b

Culture col. no. DSM 19972 CECT 7334 59b JCM 18036 CIP 110039

NCBI tax ID(s) 1423777 303241

Special Collections DSMZ JCM Literature strains CIP

History DSMZ <- R. Mañes-Lázaro, Univ. Valencia, Campus Burjasot, Spain <- A. M. Rodas; 59b JCM <-- CECT 7334 <-- R. Mañes-Lázaro <-- A. M. Rodas 59b. CIP <- 2009, CECT <- R. Manes, ENOLAB, Valencia, Spain <- A.M. Rodas: strain 59b

Links

Liquorilactobacillus oeni 59b is a microaerophile, Gram-positive, motile bacterium that was isolated from Bobal wine.

Gram-positive motile rod-shaped microaerophile genome sequence 16S sequence Bacteria

Name and taxonomic classification

SI-ID 363647

CECT 7334,DSM 19972,LMG 26189,JCM 18036,CIP 110039

@ref 20215

Last LPSN update 2026-05-29 08:26:28

Domain Bacteria

Phylum Bacillota

Class Bacilli

Order Lactobacillales

Family Lactobacillaceae

Genus Liquorilactobacillus

Species Liquorilactobacillus oeni

Full scientific name Liquorilactobacillus oeni (Mañes-Lázaro et al. 2009) Zheng et al. 2020

Synonyms (1)

Lactobacillus oeni

Morphology

Cell morphology

@ref	Gram stain	Cell length	Cell width	Cell shape	Confidence
29156	positive	2.36 µm	0.8 µm	rod-shaped
121400	positive			rod-shaped
125438	positive				90.395

Culture and growth conditions

Culture medium

@ref	Name	Medium link	Composition
8347	MRS MEDIUM (DSMZ Medium 11)	Medium recipe at MediaDive	Name: MRS MEDIUM (DSMZ Medium 11) Composition: Glucose 20.0 g/l Casein peptone 10.0 g/l Meat extract 10.0 g/l Na-acetate 5.0 g/l Yeast extract 5.0 g/l (NH4)3 citrate 2.0 g/l K2HPO4 2.0 g/l Tween 80 1.0 g/l MgSO4 x 7 H2O 0.2 g/l MnSO4 x H2O 0.05 g/l Distilled water
33342	MEDIUM 40- for Lactobacillus and Leuconostoc		Distilled water make up to (1000.000 ml);Man Rogosa Sharp agar (68.000 g)
121400	CIP Medium 40	Medium recipe at CIP

Culture temp

@ref	Growth	Type	Temperature (°C)
8347	positive	growth	30
29156	positive	growth	15-45
29156	positive	optimum	30
33342	positive	growth	37
67770	positive	growth	28
121400	positive	growth	15-37
121400	negative	growth	45

Culture pH

@ref	Ability	Type	PH
29156	positive	optimum	5.8
29156	positive	growth	4.5-8.0

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance	Confidence
8347	microaerophile
29156	anaerobe
121400	facultative anaerobe
125439	obligate aerobe	95.026

Spore formation

29156

Spore formationno

Halophily

@ref	Salt	Growth	Tested relation	Concentration
29156	NaCl	positive	optimum	5 %

Observation

29156

Observationaggregates in chains

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
68371	27613 ChEBI	amygdalin	-	builds acid from	from API 50CH acid
68371	18305 ChEBI	arbutin	-	builds acid from	from API 50CH acid
68371	17057 ChEBI	cellobiose	-	builds acid from	from API 50CH acid
68371	17108 ChEBI	D-arabinose	-	builds acid from	from API 50CH acid
68371	18333 ChEBI	D-arabitol	-	builds acid from	from API 50CH acid
68371	15824 ChEBI	D-fructose	+	builds acid from	from API 50CH acid
68371	28847 ChEBI	D-fucose	-	builds acid from	from API 50CH acid
68371	12936 ChEBI	D-galactose	-	builds acid from	from API 50CH acid
68371	17634 ChEBI	D-glucose	+	builds acid from	from API 50CH acid
68371	62318 ChEBI	D-lyxose	-	builds acid from	from API 50CH acid
68371	16899 ChEBI	D-mannitol	+	builds acid from	from API 50CH acid
68371	16024 ChEBI	D-mannose	+	builds acid from	from API 50CH acid
68371	16988 ChEBI	D-ribose	-	builds acid from	from API 50CH acid
68371	17924 ChEBI	D-sorbitol	+	builds acid from	from API 50CH acid
68371	16443 ChEBI	D-tagatose	-	builds acid from	from API 50CH acid
68371	65327 ChEBI	D-xylose	-	builds acid from	from API 50CH acid
68371	17113 ChEBI	erythritol	-	builds acid from	from API 50CH acid
29156	4853 ChEBI	esculin	+	hydrolysis
29156	28757 ChEBI	fructose	+	carbon source
68371	16813 ChEBI	galactitol	-	builds acid from	from API 50CH acid
68371	28066 ChEBI	gentiobiose	-	builds acid from	from API 50CH acid
68371	24265 ChEBI	gluconate	-	builds acid from	from API 50CH acid
29156	17234 ChEBI	glucose	+	carbon source
68371	17754 ChEBI	glycerol	-	builds acid from	from API 50CH acid
68371	28087 ChEBI	glycogen	-	builds acid from	from API 50CH acid
68371	15443 ChEBI	inulin	-	builds acid from	from API 50CH acid
68371	30849 ChEBI	L-arabinose	-	builds acid from	from API 50CH acid
68371	18403 ChEBI	L-arabitol	-	builds acid from	from API 50CH acid
68371	18287 ChEBI	L-fucose	-	builds acid from	from API 50CH acid
68371	62345 ChEBI	L-rhamnose	-	builds acid from	from API 50CH acid
68371	17266 ChEBI	L-sorbose	+	builds acid from	from API 50CH acid
68371	65328 ChEBI	L-xylose	-	builds acid from	from API 50CH acid
68371	17716 ChEBI	lactose	-	builds acid from	from API 50CH acid
68371	17306 ChEBI	maltose	-	builds acid from	from API 50CH acid
29156	29864 ChEBI	mannitol	+	carbon source
29156	37684 ChEBI	mannose	+	carbon source
68371	6731 ChEBI	melezitose	-	builds acid from	from API 50CH acid
68371	28053 ChEBI	melibiose	-	builds acid from	from API 50CH acid
68371	320061 ChEBI	methyl alpha-D-glucopyranoside	+	builds acid from	from API 50CH acid
68371	43943 ChEBI	methyl alpha-D-mannoside	-	builds acid from	from API 50CH acid
68371	74863 ChEBI	methyl beta-D-xylopyranoside	-	builds acid from	from API 50CH acid
68371	17268 ChEBI	myo-inositol	-	builds acid from	from API 50CH acid
29156	506227 ChEBI	N-acetylglucosamine	+	carbon source
68371	59640 ChEBI	N-acetylglucosamine	+	builds acid from	from API 50CH acid
121400	17632 ChEBI	nitrate	-	reduction
121400	17632 ChEBI	nitrate	+	respiration
121400	16301 ChEBI	nitrite	-	reduction
68371	0 ChEBI	Potassium 2-ketogluconate	-	builds acid from	from API 50CH acid
68371	0 ChEBI	Potassium 5-ketogluconate	-	builds acid from	from API 50CH acid
68371	16634 ChEBI	raffinose	-	builds acid from	from API 50CH acid
68371	15963 ChEBI	ribitol	-	builds acid from	from API 50CH acid
68371	17814 ChEBI	salicin	-	builds acid from	from API 50CH acid
29156	30911 ChEBI	sorbitol	+	carbon source
68371	28017 ChEBI	starch	-	builds acid from	from API 50CH acid
29156	17992 ChEBI	sucrose	+	carbon source
68371	17992 ChEBI	sucrose	-	builds acid from	from API 50CH acid
29156	27082 ChEBI	trehalose	+	carbon source
68371	27082 ChEBI	trehalose	+	builds acid from	from API 50CH acid
68371	32528 ChEBI	turanose	-	builds acid from	from API 50CH acid
68371	17151 ChEBI	xylitol	-	builds acid from	from API 50CH acid

Metabolite tests

@ref	Chebi-ID	Metabolite	Voges-proskauer-test
121400	15688 ChEBI	acetoin	-

Enzymes

@ref	Value	Activity	Ec
68382	acid phosphatase	+	3.1.3.2	from API zym
121400	alcohol dehydrogenase	-	1.1.1.1
68382	alkaline phosphatase	+	3.1.3.1	from API zym
68382	alpha-chymotrypsin	-	3.4.21.1	from API zym
68382	alpha-fucosidase	-	3.2.1.51	from API zym
68382	alpha-galactosidase	-	3.2.1.22	from API zym
68382	alpha-glucosidase	+	3.2.1.20	from API zym
68382	alpha-mannosidase	-	3.2.1.24	from API zym
68382	beta-galactosidase	-	3.2.1.23	from API zym
68382	beta-glucosidase	-	3.2.1.21	from API zym
68382	beta-glucuronidase	-	3.2.1.31	from API zym
121400	catalase	-	1.11.1.6
68382	cystine arylamidase	-	3.4.11.3	from API zym
68382	esterase (C 4)	-		from API zym
68382	esterase lipase (C 8)	-		from API zym
68382	leucine arylamidase	+	3.4.11.1	from API zym
68382	lipase (C 14)	-		from API zym
121400	lysine decarboxylase	-	4.1.1.18
68382	N-acetyl-beta-glucosaminidase	-	3.2.1.52	from API zym
68382	naphthol-AS-BI-phosphohydrolase	+		from API zym
121400	ornithine decarboxylase	-	4.1.1.17
121400	oxidase	-
68382	trypsin	-	3.4.21.4	from API zym
68382	valine arylamidase	+		from API zym

Pathway annotation

Computationally determined by

@ref	pathway	enzyme coverage	annotated reactions
66794	L-lactaldehyde degradation	100	3 of 3
66794	UDP-GlcNAc biosynthesis	100	3 of 3
66794	CDP-diacylglycerol biosynthesis	100	2 of 2
66794	acetoin degradation	100	3 of 3
66794	coenzyme A metabolism	100	4 of 4
66794	ribulose monophosphate pathway	100	2 of 2
66794	palmitate biosynthesis	100	22 of 22
66794	cis-vaccenate biosynthesis	100	2 of 2
66794	acetate fermentation	100	4 of 4
66794	ppGpp biosynthesis	100	4 of 4
66794	folate polyglutamylation	100	1 of 1
66794	anapleurotic synthesis of oxalacetate	100	1 of 1
66794	aspartate and asparagine metabolism	88.89	8 of 9
66794	pentose phosphate pathway	81.82	9 of 11
66794	methylglyoxal degradation	80	4 of 5
66794	threonine metabolism	80	8 of 10
66794	peptidoglycan biosynthesis	80	12 of 15
66794	glutamate and glutamine metabolism	78.57	22 of 28
66794	pyrimidine metabolism	77.78	35 of 45
66794	serine metabolism	77.78	7 of 9
66794	purine metabolism	75.53	71 of 94
66794	degradation of sugar alcohols	75	12 of 16
66794	photosynthesis	71.43	10 of 14
66794	cardiolipin biosynthesis	71.43	5 of 7
66794	glycolysis	70.59	12 of 17
66794	phenylalanine metabolism	69.23	9 of 13
66794	cyanate degradation	66.67	2 of 3
66794	valine metabolism	66.67	6 of 9
66794	proline metabolism	63.64	7 of 11
66794	dTDPLrhamnose biosynthesis	62.5	5 of 8
66794	ketogluconate metabolism	62.5	5 of 8
66794	C4 and CAM-carbon fixation	62.5	5 of 8
66794	vitamin B1 metabolism	61.54	8 of 13
66794	methionine metabolism	61.54	16 of 26
66794	NAD metabolism	61.11	11 of 18
66794	non-pathway related	60.53	23 of 38
66794	glycogen metabolism	60	3 of 5
66794	propanol degradation	57.14	4 of 7
66794	ubiquinone biosynthesis	57.14	4 of 7
66794	oxidative phosphorylation	56.04	51 of 91
66794	cysteine metabolism	55.56	10 of 18
66794	alanine metabolism	55.17	16 of 29
66794	histidine metabolism	55.17	16 of 29
66794	urea cycle	53.85	7 of 13
66794	cyclohexanol degradation	50	2 of 4
66794	lactate fermentation	50	2 of 4
66794	gluconeogenesis	50	4 of 8
66794	arginine metabolism	50	12 of 24
66794	sulfopterin metabolism	50	2 of 4
66794	glycolate and glyoxylate degradation	50	3 of 6
66794	suberin monomers biosynthesis	50	1 of 2
66794	glutathione metabolism	50	7 of 14
66794	ethanol fermentation	50	1 of 2
66794	butanoate fermentation	50	2 of 4
66794	lysine metabolism	50	21 of 42
66794	isoleucine metabolism	50	4 of 8
66794	phenylmercury acetate degradation	50	1 of 2
66794	degradation of aromatic, nitrogen containing compounds	50	6 of 12
66794	isoprenoid biosynthesis	46.15	12 of 26
66794	CO2 fixation in Crenarchaeota	44.44	4 of 9
66794	d-mannose degradation	44.44	4 of 9
66794	reductive acetyl coenzyme A pathway	42.86	3 of 7
66794	tetrahydrofolate metabolism	42.86	6 of 14
66794	citric acid cycle	42.86	6 of 14
66794	mevalonate metabolism	42.86	3 of 7
66794	arachidonate biosynthesis	40	2 of 5
66794	Entner Doudoroff pathway	40	4 of 10
66794	propionate fermentation	40	4 of 10
66794	lipoate biosynthesis	40	2 of 5
66794	glycine metabolism	40	4 of 10
66794	metabolism of amino sugars and derivatives	40	2 of 5
66794	tryptophan metabolism	39.47	15 of 38
66794	lipid metabolism	38.71	12 of 31
66794	leucine metabolism	38.46	5 of 13
66794	6-hydroxymethyl-dihydropterin diphosphate biosynthesis	37.5	3 of 8
66794	vitamin B6 metabolism	36.36	4 of 11
66794	formaldehyde oxidation	33.33	1 of 3
66794	sphingosine metabolism	33.33	2 of 6
66794	acetyl CoA biosynthesis	33.33	1 of 3
66794	selenocysteine biosynthesis	33.33	2 of 6
66794	octane oxidation	33.33	1 of 3
66794	IAA biosynthesis	33.33	1 of 3
66794	flavin biosynthesis	33.33	5 of 15
66794	lipid A biosynthesis	33.33	3 of 9
66794	degradation of pentoses	32.14	9 of 28
66794	myo-inositol biosynthesis	30	3 of 10
66794	starch degradation	30	3 of 10
66794	tyrosine metabolism	28.57	4 of 14
66794	degradation of hexoses	27.78	5 of 18
66794	d-xylose degradation	27.27	3 of 11
66794	metabolism of disaccharids	27.27	3 of 11
66794	3-phenylpropionate degradation	26.67	4 of 15
66794	phenol degradation	25	5 of 20
66794	toluene degradation	25	1 of 4
66794	carnitine metabolism	25	2 of 8
66794	glycogen biosynthesis	25	1 of 4
66794	CMP-KDO biosynthesis	25	1 of 4
66794	androgen and estrogen metabolism	25	4 of 16
66794	phenylpropanoid biosynthesis	23.08	3 of 13
66794	ascorbate metabolism	22.73	5 of 22
66794	nitrate assimilation	22.22	2 of 9
66794	chorismate metabolism	22.22	2 of 9
66794	arachidonic acid metabolism	22.22	4 of 18

API zym

@ref	Control	Alkaline phosphatase	Esterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	Lipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	Trypsin	alpha- Chymotrypsin	Acid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	alpha- Galactosidase	beta- Galactosidase	beta- Glucuronidase	alpha- Glucosidase	beta- Glucosidase	N-acetyl-beta- glucosaminidase	alpha- Mannosidase	alpha- Fucosidase
121400	-	+	-	-	-	+	+	-	-	-	+	+	-	-	-	+	-	-	-	-

API 50CHac

@ref	ControlQ	GLY	ERY	DARA	LARA	RIB	DXYL	LXYL	ADO	MDX	GAL	GLU	FRU	MNE	SBE	RHA	DUL	INO	MAN	SOR	MDM	MDG	NAG	AMY	ARB	ESC	SAL	CEL	MAL	LAC	MEL	SAC	TRE	INU	MLZ	RAF	AMD	GLYG	XLT	GEN	TUR	LYX	TAG	DFUC	LFUC	DARL	LARL	GNT	2KG	5KG
8347	-	-	-	-	-	-	-	-	-	-	-	+	+	+	+	-	-	-	+	+	-	+	+	-	-	+/-	-	-	-	-	-	-	+	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Engineered	#Food production	#Fermented
#Engineered	#Food production	#Beverage

Isolation

@ref	Sample type	Geographic location	Country	Country ISO 3 Code	Continent
8347	Bobal wine	Utiel-Requena, Sinarcas winery	Spain	ESP	Europe
67770	Bobal wine		Spain	ESP	Europe
121400	Food, Bobal grape wines		Spain	ESP	Europe

Taxonmaps

69479

Global distribution of 16S sequence LC521975 (>99% sequence identity) for Liquorilactobacillus oeni from Microbeatlas

Aquatic Samples	23
Soil Samples	9
Animal Samples	340
Plant Samples	42
Total Samples	414

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
8347	1	Risk group (German classification) According to TRBA 466
121400	1	Risk group (French classification) According to TRBA 466

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
67770	ASM143627v1 assembly for Liquorilactobacillus oeni DSM 19972	scaffold	GCA_001436275	1423777.3	2681813159	1423777	68.81

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
8347	Lactobacillus oeni strain 59b 16S ribosomal RNA gene, complete sequence	AY681127	1556		303241
67770	Lactobacillus oeni JCM 18036 gene for 16S rRNA, partial sequence	LC521975	1500		303241

GC content

@ref	GC-content (mol%)	Method
8347	37.17±0.16	high performance liquid chromatography (HPLC)
29156	37.17
67770	37.01-37.33	high performance liquid chromatography (HPLC)
67770	37.3	genome sequence analysis

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: ASM143627v1 assembly for Liquorilactobacillus oeni DSM 19972
NCBI: GCA_001436275

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	oxygen_tolerance	BacteriaNetⓘ	obligate aerobe	95.03	no
125439	gram_stain	BacteriaNetⓘ	positive	67.20	no
125439	motility	BacteriaNetⓘ	yes	41.07	no
125439	spore_formation	BacteriaNetⓘ	no	70.68	no

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Lactobacillus oeni DSM 19972
PATRIC: 1423777.3

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	yes	90.40	yes
125438	anaerobic	anaerobicⓘ	no	85.25	no
125438	spore-forming	spore-formingⓘ	no	80.15	yes
125438	aerobic	aerobicⓘ	no	95.21	yes
125438	thermophilic	thermophileⓘ	no	93.89	yes
125438	flagellated	motile2+ⓘ	yes	55.50	no

Literature

Topic	Title	Authors	Journal	DOI	Year
Genetics	Detection and genomic characterization of motility in Lactobacillus curvatus: confirmation of motility in a species outside the Lactobacillus salivarius clade.	Cousin FJ, Lynch SM, Harris HM, McCann A, Lynch DB, Neville BA, Irisawa T, Okada S, Endo A, O'Toole PW.	Appl Environ Microbiol	10.1128/aem.03594-14	2015
Phylogeny	Lactobacillus oeni sp. nov., from wine.	Manes-Lazaro R, Ferrer S, Rossello-Mora R, Pardo I	Int J Syst Evol Microbiol	10.1099/ijs.0.007567-0	2009

References

#8347	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 19972
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#25580	IJSEM 2010 2009 ( DOI 10.1099/ijs.0.007567-0 , PubMed 19567555 )
#29156	Barberan A, Caceres Velazquez H, Jones S, Fierer N.: Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information. mSphere 2: 2017 ( DOI 10.1128/mSphere.00237-17 , PubMed 28776041 ) - originally annotated from #25580
#33342	; Curators of the CIP;
#66794	Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg: BRENDA, the ELIXIR core data resource in 2021: new developments and updates. Nucleic Acids Res. 49: D498 - D508 2020 ( DOI 10.1093/nar/gkaa1025 , PubMed 33211880 )
#67770	Japan Collection of Microorganism (JCM) ; Curators of the JCM;
#68371	Automatically annotated from API 50CH acid .
#68382	Automatically annotated from API zym .
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#121400	Collection of Institut Pasteur ; Curators of the CIP; CIP 110039
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Liquorilactobacillus oeni (DSM 19972, CECT 7334, 59b)

Name and taxonomic classification

Morphology

Cell morphology

Culture and growth conditions

Culture medium

Culture temp

Culture pH

Physiology and metabolism

Oxygen tolerance

Spore formation

Halophily

Observation

Metabolite utilization

Metabolite tests

Enzymes

Pathway annotation

API zym

API 50CHac

Isolation, sampling and environmental information

Isolation source categories

Isolation

Taxonmaps

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_001436275

16S sequences

GC content

Genome-based predictions

Predictions

Literature

Literature

References

BacDive

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