Strain identifier

BacDive ID: 6676

Type strain: Yes

Species: Liquorilactobacillus oeni

Strain Designation: 59b

Strain history: CIP <- 2009, CECT <- R. Manes, ENOLAB, Valencia, Spain <- A.M. Rodas: strain 59b

NCBI tax ID(s): 1423777 (strain), 303241 (species)

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General

@ref: 8347

BacDive-ID: 6676

DSM-Number: 19972

keywords: genome sequence, 16S sequence, Bacteria, microaerophile, mesophilic, Gram-positive, motile, rod-shaped

description: Liquorilactobacillus oeni 59b is a microaerophile, mesophilic, Gram-positive bacterium that was isolated from Bobal wine.

NCBI tax id

NCBI tax idMatching level
1423777strain
303241species

strain history

@refhistory
8347<- R. Mañes-Lázaro, Univ. Valencia, Campus Burjasot, Spain <- A. M. Rodas; 59b
67770CECT 7334 <-- R. Mañes-Lázaro <-- A. M. Rodas 59b.
121400CIP <- 2009, CECT <- R. Manes, ENOLAB, Valencia, Spain <- A.M. Rodas: strain 59b

doi: 10.13145/bacdive6676.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Lactobacillales
  • family: Lactobacillaceae
  • genus: Liquorilactobacillus
  • species: Liquorilactobacillus oeni
  • full scientific name: Liquorilactobacillus oeni (Mañes-Lázaro et al. 2009) Zheng et al. 2020

  • synonyms

    • @ref: 20215
    • synonym: Lactobacillus oeni

@ref: 8347

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Lactobacillales

family: Lactobacillaceae

genus: Liquorilactobacillus

species: Liquorilactobacillus oeni

full scientific name: Liquorilactobacillus oeni (Mañes-Lázaro et al. 2009) Zheng et al. 2020

strain designation: 59b

type strain: yes

Morphology

cell morphology

@refgram staincell lengthcell widthcell shapemotilityconfidence
29156positive2.36 µm0.8 µmrod-shapedyes
69480positive99.999
121400positiverod-shapedno

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
8347MRS MEDIUM (DSMZ Medium 11)yeshttps://mediadive.dsmz.de/medium/11Name: MRS MEDIUM (DSMZ Medium 11) Composition: Glucose 20.0 g/l Casein peptone 10.0 g/l Meat extract 10.0 g/l Na-acetate 5.0 g/l Yeast extract 5.0 g/l (NH4)3 citrate 2.0 g/l K2HPO4 2.0 g/l Tween 80 1.0 g/l MgSO4 x 7 H2O 0.2 g/l MnSO4 x H2O 0.05 g/l Distilled water
33342MEDIUM 40- for Lactobacillus and LeuconostocyesDistilled water make up to (1000.000 ml);Man Rogosa Sharp agar (68.000 g)
121400CIP Medium 40yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=40

culture temp

@refgrowthtypetemperaturerange
8347positivegrowth30mesophilic
29156positivegrowth15-45
29156positiveoptimum30mesophilic
33342positivegrowth37mesophilic
67770positivegrowth28mesophilic
121400positivegrowth15-37
121400nogrowth45thermophilic

culture pH

@refabilitytypepH
29156positivegrowth4.5-8.0
29156positiveoptimum5.8

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
8347microaerophile
29156anaerobe
121400facultative anaerobe

spore formation

@refspore formationconfidence
29156no
69481no100
69480no99.982

halophily

  • @ref: 29156
  • salt: NaCl
  • growth: positive
  • tested relation: optimum
  • concentration: 5 %

observation

  • @ref: 29156
  • observation: aggregates in chains

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
2915628757fructose+carbon source
2915617234glucose+carbon source
2915629864mannitol+carbon source
2915637684mannose+carbon source
29156506227N-acetylglucosamine+carbon source
2915630911sorbitol+carbon source
2915617992sucrose+carbon source
2915627082trehalose+carbon source
291564853esculin+hydrolysis
68371Potassium 5-ketogluconate-builds acid from
68371Potassium 2-ketogluconate-builds acid from
6837124265gluconate-builds acid from
6837118403L-arabitol-builds acid from
6837118333D-arabitol-builds acid from
6837118287L-fucose-builds acid from
6837128847D-fucose-builds acid from
6837116443D-tagatose-builds acid from
6837162318D-lyxose-builds acid from
6837132528turanose-builds acid from
6837128066gentiobiose-builds acid from
6837117151xylitol-builds acid from
6837128087glycogen-builds acid from
6837128017starch-builds acid from
6837116634raffinose-builds acid from
683716731melezitose-builds acid from
6837115443inulin-builds acid from
6837127082trehalose+builds acid from
6837117992sucrose-builds acid from
6837128053melibiose-builds acid from
6837117716lactose-builds acid from
6837117306maltose-builds acid from
6837117057cellobiose-builds acid from
6837117814salicin-builds acid from
6837118305arbutin-builds acid from
6837127613amygdalin-builds acid from
6837159640N-acetylglucosamine+builds acid from
68371320061methyl alpha-D-glucopyranoside+builds acid from
6837143943methyl alpha-D-mannoside-builds acid from
6837117924D-sorbitol+builds acid from
6837116899D-mannitol+builds acid from
6837117268myo-inositol-builds acid from
6837116813galactitol-builds acid from
6837162345L-rhamnose-builds acid from
6837117266L-sorbose+builds acid from
6837116024D-mannose+builds acid from
6837115824D-fructose+builds acid from
6837117634D-glucose+builds acid from
6837112936D-galactose-builds acid from
6837174863methyl beta-D-xylopyranoside-builds acid from
6837115963ribitol-builds acid from
6837165328L-xylose-builds acid from
6837165327D-xylose-builds acid from
6837116988D-ribose-builds acid from
6837130849L-arabinose-builds acid from
6837117108D-arabinose-builds acid from
6837117113erythritol-builds acid from
6837117754glycerol-builds acid from
12140017632nitrate-reduction
12140016301nitrite-reduction
12140017632nitrate+respiration

metabolite tests

  • @ref: 121400
  • Chebi-ID: 15688
  • metabolite: acetoin
  • voges-proskauer-test: -

enzymes

@refvalueactivityec
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382beta-glucosidase-3.2.1.21
68382alpha-glucosidase+3.2.1.20
68382beta-glucuronidase-3.2.1.31
68382beta-galactosidase-3.2.1.23
68382alpha-galactosidase-3.2.1.22
68382naphthol-AS-BI-phosphohydrolase+
68382acid phosphatase+3.1.3.2
68382alpha-chymotrypsin-3.4.21.1
68382trypsin-3.4.21.4
68382cystine arylamidase-3.4.11.3
68382valine arylamidase+
68382leucine arylamidase+3.4.11.1
68382lipase (C 14)-
68382esterase lipase (C 8)-
68382esterase (C 4)-
68382alkaline phosphatase+3.1.3.1
121400oxidase-
121400alcohol dehydrogenase-1.1.1.1
121400catalase-1.11.1.6
121400lysine decarboxylase-4.1.1.18
121400ornithine decarboxylase-4.1.1.17

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
121400-+---++---++---+----

API 50CHac

@refQGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
8347-----------++++---++-++--+/-------+-----------------

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinent
8347Bobal wineUtiel-Requena, Sinarcas winerySpainESPEurope
67770Bobal wineSpainESPEurope
121400Food, Bobal grape winesSpainESPEurope

isolation source categories

Cat1Cat2Cat3
#Engineered#Food production#Fermented
#Engineered#Food production#Beverage

taxonmaps

  • @ref: 69479
  • File name: preview.99_8166.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_45;96_1207;97_1430;98_5447;99_8166&stattab=map
  • Last taxonomy: Liquorilactobacillus oeni
  • 16S sequence: LC521975
  • Sequence Identity:
  • Total samples: 414
  • soil counts: 9
  • aquatic counts: 23
  • animal counts: 340
  • plant counts: 42

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
83471Risk group (German classification)
1214001Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
8347Lactobacillus oeni strain 59b 16S ribosomal RNA gene, complete sequenceAY6811271556ena303241
67770Lactobacillus oeni JCM 18036 gene for 16S rRNA, partial sequenceLC5219751500ena303241

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Lactobacillus oeni DSM 199721423777.3wgspatric1423777
66792Liquorilactobacillus oeni DSM 199722681813159draftimg1423777
67770Liquorilactobacillus oeni DSM 19972GCA_001436275scaffoldncbi1423777

GC content

@refGC-contentmethod
834737.17±0.16high performance liquid chromatography (HPLC)
2915637.17
6777037.01-37.33high performance liquid chromatography (HPLC)
6777037.3genome sequence analysis

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingno100no
motileyes66.732no
gram-positiveyes93.74no
anaerobicno85.982no
halophileyes74.699no
spore-formingno92.46no
thermophileno97.753no
glucose-utilyes91.239yes
aerobicno97.241no
flagellatedno84.584no
glucose-fermentyes86.033no

External links

@ref: 8347

culture collection no.: DSM 19972, CECT 7334, JCM 18036, CIP 110039

straininfo link

  • @ref: 76150
  • straininfo: 363647

literature

  • topic: Phylogeny
  • Pubmed-ID: 19567555
  • title: Lactobacillus oeni sp. nov., from wine.
  • authors: Manes-Lazaro R, Ferrer S, Rossello-Mora R, Pardo I
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijs.0.007567-0
  • year: 2009
  • mesh: Anaerobiosis, Bacterial Typing Techniques, Catalase/metabolism, Cluster Analysis, DNA Fingerprinting/methods, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/chemistry/genetics, Lactobacillus/*classification/genetics/*isolation & purification/physiology, Molecular Sequence Data, Nucleic Acid Hybridization, Phylogeny, Polymorphism, Restriction Fragment Length, RNA, Ribosomal, 16S/genetics, Random Amplified Polymorphic DNA Technique, Sequence Analysis, DNA, Spain, Wine/*microbiology
  • topic2: Genetics

Reference

@idauthorscataloguedoi/urltitleID_cross_referencepubmedjournal
8347Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 19972)https://www.dsmz.de/collection/catalogue/details/culture/DSM-19972
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
29156Barberan A, Caceres Velazquez H, Jones S, Fierer N.10.1128/mSphere.00237-17Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information2558028776041
33342Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/7852
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
66794Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg10.1093/nar/gkaa1025BRENDA, the ELIXIR core data resource in 2021: new developments and updates33211880Nucleic Acids Res. 49: D498-D508 2020
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68371Automatically annotated from API 50CH acid
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
76150Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID363647.1StrainInfo: A central database for resolving microbial strain identifiers
121400Curators of the CIPCollection of Institut Pasteur (CIP 110039)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20110039