Workshop and Q&A October 6th

Strain identifier

BacDive ID: 6676

Type strain: Yes

Species: Liquorilactobacillus oeni

Strain Designation: 59b

Strain history: CECT 7334 <-- R. Mañes-Lázaro <-- A. M. Rodas 59b.

For citation purpose refer to the digital object identifier (doi) of the current version.
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General

@ref: 8347

BacDive-ID: 6676

DSM-Number: 19972

keywords: Gram-positive, mesophilic, microaerophile, Bacteria, 16S sequence, genome sequence, motile, rod-shaped

description: Liquorilactobacillus oeni 59b is a microaerophile, mesophilic, Gram-positive bacterium that was isolated from Bobal wine.

NCBI tax id

NCBI tax idMatching level
303241species
1423777strain

strain history

doi: 10.13145/bacdive6676.20220920.7

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Lactobacillales
  • family: Lactobacillaceae
  • genus: Liquorilactobacillus
  • species: Liquorilactobacillus oeni
  • full scientific name: Liquorilactobacillus oeni (Mañes-Lázaro et al. 2009) Zheng et al. 2020
  • synonyms

    • @ref: 20215
    • synonym: Lactobacillus oeni

@ref: 8347

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Lactobacillales

family: Lactobacillaceae

genus: Liquorilactobacillus

species: Liquorilactobacillus oeni

full scientific name: Liquorilactobacillus oeni (Mañes-Lázaro et al. 2009) Zheng et al. 2020

strain designation: 59b

type strain: yes

Morphology

cell morphology

  • @ref: 29156
  • gram stain: positive
  • cell length: 2.36 µm
  • cell width: 0.8 µm
  • cell shape: rod-shaped
  • motility: yes

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
8347MRS MEDIUM (DSMZ Medium 11)yeshttps://bacmedia.dsmz.de/medium/11Name: MRS MEDIUM (DSMZ Medium 11) Composition: Glucose 20.0 g/l Casein peptone 10.0 g/l Meat extract 10.0 g/l Na-acetate 5.0 g/l Yeast extract 5.0 g/l (NH4)3 citrate 2.0 g/l K2HPO4 2.0 g/l Tween 80 1.0 g/l MgSO4 x 7 H2O 0.2 g/l MnSO4 x H2O 0.05 g/l Distilled water
33342MEDIUM 40- for Lactobacillus and LeuconostocyesDistilled water make up to (1000.000 ml);Man Rogosa Sharp agar (68.000 g)

culture temp

@refgrowthtypetemperaturerange
8347positivegrowth30mesophilic
29156positivegrowth15-45
29156positiveoptimum30mesophilic
33342positivegrowth37mesophilic
67770positivegrowth28mesophilic

culture pH

@refabilitytypepH
29156positivegrowth4.5-8.0
29156positiveoptimum5.8

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
8347microaerophile
29156anaerobe

spore formation

  • @ref: 29156
  • spore formation: no

halophily

  • @ref: 29156
  • salt: NaCl
  • growth: positive
  • tested relation: optimum
  • concentration: 5 %

observation

  • @ref: 29156
  • observation: aggregates in chains

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
2915628757fructose+carbon source
2915617234glucose+carbon source
2915629864mannitol+carbon source
2915637684mannose+carbon source
29156506227N-acetylglucosamine+carbon source
2915630911sorbitol+carbon source
2915617992sucrose+carbon source
2915627082trehalose+carbon source
291564853esculin+hydrolysis
68371Potassium 5-ketogluconate-builds acid from
68371Potassium 2-ketogluconate-builds acid from
6837124265gluconate-builds acid from
6837118403L-arabitol-builds acid from
6837118333D-arabitol-builds acid from
6837118287L-fucose-builds acid from
6837128847D-fucose-builds acid from
6837116443D-tagatose-builds acid from
6837162318D-lyxose-builds acid from
6837132528turanose-builds acid from
6837128066gentiobiose-builds acid from
6837117151xylitol-builds acid from
6837128087glycogen-builds acid from
6837128017starch-builds acid from
6837116634raffinose-builds acid from
683716731melezitose-builds acid from
6837115443inulin-builds acid from
6837127082trehalose+builds acid from
6837117992sucrose-builds acid from
6837128053melibiose-builds acid from
6837117716lactose-builds acid from
6837117306maltose-builds acid from
6837117057cellobiose-builds acid from
6837117814salicin-builds acid from
6837118305arbutin-builds acid from
6837127613amygdalin-builds acid from
6837159640N-acetylglucosamine+builds acid from
68371320061methyl alpha-D-glucopyranoside+builds acid from
6837143943methyl alpha-D-mannoside-builds acid from
6837117924D-sorbitol+builds acid from
6837116899D-mannitol+builds acid from
6837117268myo-inositol-builds acid from
6837116813galactitol-builds acid from
6837162345L-rhamnose-builds acid from
6837117266L-sorbose+builds acid from
6837116024D-mannose+builds acid from
6837115824D-fructose+builds acid from
6837117634D-glucose+builds acid from
6837112936D-galactose-builds acid from
6837174863methyl beta-D-xylopyranoside-builds acid from
6837115963ribitol-builds acid from
6837165328L-xylose-builds acid from
6837165327D-xylose-builds acid from
6837116988D-ribose-builds acid from
6837130849L-arabinose-builds acid from
6837117108D-arabinose-builds acid from
6837117113erythritol-builds acid from
6837117754glycerol-builds acid from

API 50CHac

@refQGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
8347-----------++++---++-++--+/-------+-----------------

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinent
8347Bobal wineUtiel-Requena, Sinarcas winerySpainESPEurope
67770Bobal wineSpainESPEurope

isolation source categories

Cat1Cat2Cat3
#Engineered#Food production#Fermented
#Engineered#Food production#Beverage

Safety information

risk assessment

  • @ref: 8347
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
8347Lactobacillus oeni strain 59b 16S ribosomal RNA gene, complete sequenceAY6811271556ena303241
67770Lactobacillus oeni JCM 18036 gene for 16S rRNA, partial sequenceLC5219751500ena303241

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Liquorilactobacillus oeni DSM 19972 DSM 19972GCA_001436275scaffoldncbi1423777
66792Lactobacillus oeni DSM 199721423777.3wgspatric1423777
66792Liquorilactobacillus oeni DSM 199722681813159draftimg1423777
67770Liquorilactobacillus oeni DSM 19972, whole genome shotgun sequencing projectAZEH00000000ncbi1423777

GC content

@refGC-contentmethod
834737.17±0.16high performance liquid chromatography (HPLC)
2915637.17
6777037.01-37.33high performance liquid chromatography (HPLC)
6777037.3genome sequence analysis

External links

@ref: 8347

culture collection no.: DSM 19972, CECT 7334, JCM 18036

straininfo link

@refpassport
20218http://www.straininfo.net/strains/843676
20218http://www.straininfo.net/strains/843675

literature

  • topic: Phylogeny
  • Pubmed-ID: 19567555
  • title: Lactobacillus oeni sp. nov., from wine.
  • authors: Manes-Lazaro R, Ferrer S, Rossello-Mora R, Pardo I
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijs.0.007567-0
  • year: 2009
  • mesh: Anaerobiosis, Bacterial Typing Techniques, Catalase/metabolism, Cluster Analysis, DNA Fingerprinting/methods, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/chemistry/genetics, Lactobacillus/*classification/genetics/*isolation & purification/physiology, Molecular Sequence Data, Nucleic Acid Hybridization, Phylogeny, Polymorphism, Restriction Fragment Length, RNA, Ribosomal, 16S/genetics, Random Amplified Polymorphic DNA Technique, Sequence Analysis, DNA, Spain, Wine/*microbiology
  • topic2: Genetics

Reference

@idauthorscataloguedoi/urltitlejournalpubmedID_cross_reference
8347Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 19972)https://www.dsmz.de/collection/catalogue/details/culture/DSM-19972
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
2558010.1099/ijs.0.007567-019567555
29156Barberan A, Caceres Velazquez H, Jones S, Fierer N.10.1128/mSphere.00237-17Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information2877604125580
33342Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/7852
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
66794Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg10.1093/nar/gkaa1025BRENDA, the ELIXIR core data resource in 2021: new developments and updatesNucleic Acids Res. 49: D498-D508 202033211880
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68371Automatically annotated from API 50CH acid