Ligilactobacillus hayakitensis (DSM 18933, CIP 109885, JCM 14209)

Name: Ligilactobacillus hayakitensis (DSM 18933, CIP 109885, JCM 14209)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 6675

Type strain

Culture col. no. DSM 18933 CIP 109885 JCM 14209 CCUG 56036 KBL 13 1 more

NCBI tax ID(s) 396716

Special Collections DSMZ CCUG JCM KCTC CIP Literature strains

History DSMZ <- Hidetoshi Morita <- T. Mitsuoka, KBL13 JCM <-- H. Morita KBL13. CIP <- 2008, JCM <- 2006, H. Morita, Azabu Univ., Kanagawa, Japan <- T. Mitsuoka, KBL13: strain 14209

Links

Ligilactobacillus hayakitensis DSM 18933 is an anaerobe, Gram-positive, rod-shaped bacterium that was isolated from faeces of thoroughbred .

Gram-positive rod-shaped anaerobe genome sequence 16S sequence Bacteria

Name and taxonomic classification

SI-ID 323249

DSM 18933,JCM 14209,LMG 24490,CIP 109885,CCUG 56036

@ref 20215

Last LPSN update 2026-05-29 08:26:28

Domain Bacteria

Phylum Bacillota

Class Bacilli

Order Lactobacillales

Family Lactobacillaceae

Genus Ligilactobacillus

Species Ligilactobacillus hayakitensis

Full scientific name Ligilactobacillus hayakitensis (Morita et al. 2007) Zheng et al. 2020

Synonyms (1)

Lactobacillus hayakitensis

Morphology

Cell morphology

@ref	Gram stain	Cell length	Cell width	Cell shape	Confidence
32211	positive	4 µm	1.25 µm	rod-shaped
119852	positive			rod-shaped
125438					93
125438	positive				93.424

Colony morphology

60836

Incubation period2 days

Culture and growth conditions

Culture medium

@ref	Name	Medium link	Composition
7791	MRS MEDIUM (DSMZ Medium 11)	Medium recipe at MediaDive	Name: MRS MEDIUM (DSMZ Medium 11) Composition: Glucose 20.0 g/l Casein peptone 10.0 g/l Meat extract 10.0 g/l Na-acetate 5.0 g/l Yeast extract 5.0 g/l (NH4)3 citrate 2.0 g/l K2HPO4 2.0 g/l Tween 80 1.0 g/l MgSO4 x 7 H2O 0.2 g/l MnSO4 x H2O 0.05 g/l Distilled water
36198	MEDIUM 40- for Lactobacillus and Leuconostoc		Distilled water make up to (1000.000 ml);Man Rogosa Sharp agar (68.000 g)
119852	CIP Medium 40	Medium recipe at CIP

Culture temp

@ref	Growth	Type	Temperature (°C)
7791	positive	growth	37
32211	positive	optimum	37
36198	positive	growth	37
60836	positive	growth	37
67770	positive	growth	37
119852	positive	growth	25-37
119852	negative	growth	15
119852	negative	growth	45

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance
7791	anaerobe
32211	facultative anaerobe
60836	anaerobe
119852	facultative anaerobe

Spore formation

@ref	Spore formation	Confidence
32211
125439		92.854
125438		92.23

Halophily

@ref	Salt	Growth	Tested relation	Concentration
32211	NaCl	positive	optimum	3 %

Murein

@ref	Murein short key	Type
7791	A11.31	A4alpha L-Lys-D-Asp

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
32211	17057 ChEBI	cellobiose	+	carbon source
32211	4853 ChEBI	esculin	+	hydrolysis
32211	28757 ChEBI	fructose	+	carbon source
32211	17234 ChEBI	glucose	+	carbon source
32211	17306 ChEBI	maltose	+	carbon source
32211	29864 ChEBI	mannitol	+	carbon source
32211	37684 ChEBI	mannose	+	carbon source
119852	17632 ChEBI	nitrate	-	reduction
119852	17632 ChEBI	nitrate	+	respiration
119852	16301 ChEBI	nitrite	-	reduction
32211	16634 ChEBI	raffinose	+	carbon source
32211	17814 ChEBI	salicin	+	carbon source
32211	17992 ChEBI	sucrose	+	carbon source

Metabolite tests

@ref	Chebi-ID	Metabolite	Voges-proskauer-test
119852	15688 ChEBI	acetoin	-

Enzymes

@ref	Value	Activity	Ec
68382	acid phosphatase	+	3.1.3.2	from API zym
119852	alcohol dehydrogenase	-	1.1.1.1
68382	alkaline phosphatase	+	3.1.3.1	from API zym
68382	alpha-chymotrypsin	-	3.4.21.1	from API zym
68382	alpha-fucosidase	-	3.2.1.51	from API zym
68382	alpha-galactosidase	-	3.2.1.22	from API zym
68382	alpha-glucosidase	-	3.2.1.20	from API zym
68382	alpha-mannosidase	-	3.2.1.24	from API zym
68382	beta-galactosidase	-	3.2.1.23	from API zym
68382	beta-glucosidase	+	3.2.1.21	from API zym
68382	beta-glucuronidase	-	3.2.1.31	from API zym
119852	catalase	-	1.11.1.6
68382	cystine arylamidase	-	3.4.11.3	from API zym
68382	esterase (C 4)	-		from API zym
68382	esterase lipase (C 8)	-		from API zym
68382	leucine arylamidase	+	3.4.11.1	from API zym
68382	lipase (C 14)	-		from API zym
119852	lysine decarboxylase	-	4.1.1.18
68382	N-acetyl-beta-glucosaminidase	-	3.2.1.52	from API zym
68382	naphthol-AS-BI-phosphohydrolase	+		from API zym
119852	ornithine decarboxylase	-	4.1.1.17
119852	oxidase	-
68382	trypsin	-	3.4.21.4	from API zym
68382	valine arylamidase	-		from API zym

Pathway annotation

Computationally determined by

@ref	pathway	enzyme coverage	annotated reactions
66794	coenzyme A metabolism	100	4 of 4
66794	formaldehyde oxidation	100	3 of 3
66794	cis-vaccenate biosynthesis	100	2 of 2
66794	ppGpp biosynthesis	100	4 of 4
66794	CDP-diacylglycerol biosynthesis	100	2 of 2
66794	UDP-GlcNAc biosynthesis	100	3 of 3
66794	acetoin degradation	100	3 of 3
66794	folate polyglutamylation	100	1 of 1
66794	anapleurotic synthesis of oxalacetate	100	1 of 1
66794	palmitate biosynthesis	100	22 of 22
66794	peptidoglycan biosynthesis	86.67	13 of 15
66794	aspartate and asparagine metabolism	77.78	7 of 9
66794	acetate fermentation	75	3 of 4
66794	cardiolipin biosynthesis	71.43	5 of 7
66794	vitamin B1 metabolism	69.23	9 of 13
66794	L-lactaldehyde degradation	66.67	2 of 3
66794	photosynthesis	64.29	9 of 14
66794	C4 and CAM-carbon fixation	62.5	5 of 8
66794	phenylalanine metabolism	61.54	8 of 13
66794	NAD metabolism	61.11	11 of 18
66794	threonine metabolism	60	6 of 10
66794	glycine betaine biosynthesis	60	3 of 5
66794	glycolysis	58.82	10 of 17
66794	serine metabolism	55.56	5 of 9
66794	valine metabolism	55.56	5 of 9
66794	CO2 fixation in Crenarchaeota	55.56	5 of 9
66794	pentose phosphate pathway	54.55	6 of 11
66794	starch degradation	50	5 of 10
66794	butanoate fermentation	50	2 of 4
66794	methionine metabolism	50	13 of 26
66794	glycogen biosynthesis	50	2 of 4
66794	ethanol fermentation	50	1 of 2
66794	gluconeogenesis	50	4 of 8
66794	sulfopterin metabolism	50	2 of 4
66794	suberin monomers biosynthesis	50	1 of 2
66794	phenylmercury acetate degradation	50	1 of 2
66794	purine metabolism	45.74	43 of 94
66794	non-pathway related	44.74	17 of 38
66794	d-mannose degradation	44.44	4 of 9
66794	glutathione metabolism	42.86	6 of 14
66794	oxidative phosphorylation	42.86	39 of 91
66794	propanol degradation	42.86	3 of 7
66794	glutamate and glutamine metabolism	42.86	12 of 28
66794	mevalonate metabolism	42.86	3 of 7
66794	arachidonate biosynthesis	40	2 of 5
66794	metabolism of amino sugars and derivatives	40	2 of 5
66794	lipoate biosynthesis	40	2 of 5
66794	glycogen metabolism	40	2 of 5
66794	methylglyoxal degradation	40	2 of 5
66794	lysine metabolism	38.1	16 of 42
66794	pyrimidine metabolism	37.78	17 of 45
66794	isoleucine metabolism	37.5	3 of 8
66794	ketogluconate metabolism	37.5	3 of 8
66794	6-hydroxymethyl-dihydropterin diphosphate biosynthesis	37.5	3 of 8
66794	proline metabolism	36.36	4 of 11
66794	tetrahydrofolate metabolism	35.71	5 of 14
66794	degradation of pentoses	35.71	10 of 28
66794	sphingosine metabolism	33.33	2 of 6
66794	lipid A biosynthesis	33.33	3 of 9
66794	octane oxidation	33.33	1 of 3
66794	flavin biosynthesis	33.33	5 of 15
66794	acetyl CoA biosynthesis	33.33	1 of 3
66794	glycolate and glyoxylate degradation	33.33	2 of 6
66794	IAA biosynthesis	33.33	1 of 3
66794	selenocysteine biosynthesis	33.33	2 of 6
66794	lipid metabolism	32.26	10 of 31
66794	degradation of sugar alcohols	31.25	5 of 16
66794	alanine metabolism	31.03	9 of 29
66794	isoprenoid biosynthesis	30.77	8 of 26
66794	propionate fermentation	30	3 of 10
66794	Entner Doudoroff pathway	30	3 of 10
66794	reductive acetyl coenzyme A pathway	28.57	2 of 7
66794	ubiquinone biosynthesis	28.57	2 of 7
66794	citric acid cycle	28.57	4 of 14
66794	cysteine metabolism	27.78	5 of 18
66794	degradation of hexoses	27.78	5 of 18
66794	d-xylose degradation	27.27	3 of 11
66794	metabolism of disaccharids	27.27	3 of 11
66794	tryptophan metabolism	26.32	10 of 38
66794	carnitine metabolism	25	2 of 8
66794	dTDPLrhamnose biosynthesis	25	2 of 8
66794	degradation of aromatic, nitrogen containing compounds	25	3 of 12
66794	cyclohexanol degradation	25	1 of 4
66794	lactate fermentation	25	1 of 4
66794	arginine metabolism	25	6 of 24
66794	histidine metabolism	24.14	7 of 29
66794	leucine metabolism	23.08	3 of 13
66794	urea cycle	23.08	3 of 13
66794	sulfate reduction	23.08	3 of 13
66794	nitrate assimilation	22.22	2 of 9
66794	arachidonic acid metabolism	22.22	4 of 18
66794	chorismate metabolism	22.22	2 of 9
66794	polyamine pathway	21.74	5 of 23
66794	tyrosine metabolism	21.43	3 of 14

API zym

@ref	Control	Alkaline phosphatase	Esterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	Lipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	Trypsin	alpha- Chymotrypsin	Acid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	alpha- Galactosidase	beta- Galactosidase	beta- Glucuronidase	alpha- Glucosidase	beta- Glucosidase	N-acetyl-beta- glucosaminidase	alpha- Mannosidase	alpha- Fucosidase
119852	-	+	-	-	-	+	-	-	-	-	+	+	-	-	-	-	+	-	-	-

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Host	#Mammals	#Equidae (Horse)
#Host Body Product	#Gastrointestinal tract	#Feces (Stool)

Isolation

@ref	Sample type	Geographic location	Country	Country ISO 3 Code	Continent	Isolation date
7791	faeces of thoroughbred (horse)	Hokkaido	Japan	JPN	Asia
60836	Equine feces,healthy thoroughbred		Japan	JPN	Asia
67770	Feces of a thoroughbred
119852	Animal, Horse, faeces of thoroughbred	Kanagawa	Japan	JPN	Asia	2007

Taxonmaps

69479

Global distribution of 16S sequence LC480804 (>99% sequence identity) for Ligilactobacillus hayakitensis subclade from Microbeatlas

Aquatic Samples	36
Soil Samples	12
Animal Samples	3124
Plant Samples	7
Total Samples	3179

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
7791	1	Risk group (German classification) According to TRBA 466
119852	1	Risk group (French classification) According to TRBA 466

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
67770	ASM61584v1 assembly for Ligilactobacillus hayakitensis DSM 18933 = JCM 14209	contig	GCA_000615845	1236951.3	2585427880	1423755	54.9
67770	ASM143445v1 assembly for Ligilactobacillus hayakitensis DSM 18933 = JCM 14209	scaffold	GCA_001434455	1423755.3	2681813136	1423755	50.87

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
7791	Lactobacillus hayakitensis gene for 16S ribosomal RNA, partial sequence	AB267406	1484		396716
67770	Lactobacillus hayakitensis JCM 14209 gene for 16S ribosomal RNA, partial sequence	LC480804	1500		396716

GC content

@ref	GC-content (mol%)	Method
7791	34.3
67770	34.3	high performance liquid chromatography (HPLC)

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: ASM61584v1 assembly for Ligilactobacillus hayakitensis DSM 18933 = JCM 14209
BV-BRC: 1236951.3

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	oxygen_tolerance	BacteriaNetⓘ	obligate aerobe	70.59	no
125439	gram_stain	BacteriaNetⓘ	positive	62.50	no
125439	motility	BacteriaNetⓘ	no	81.20	no
125439	spore_formation	BacteriaNetⓘ	no	92.85	no

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Lactobacillus hayakitensis DSM 18933 = JCM 14209
PATRIC: 1423755.3

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	yes	93.42	yes
125438	anaerobic	anaerobicⓘ	no	76.23	no
125438	spore-forming	spore-formingⓘ	no	92.23	yes
125438	aerobic	aerobicⓘ	no	94.01	no
125438	thermophilic	thermophileⓘ	no	94.00	yes
125438	flagellated	motile2+ⓘ	no	93.00	no

Literature

Topic	Title	Authors	Journal	DOI	Year
Metabolism	Luteibacter jiangsuensis sp. nov.: a methamidophos-degrading bacterium isolated from a methamidophos-manufacturing factory.	Wang L, Wang GL, Li SP, Jiang JD.	Curr Microbiol	10.1007/s00284-010-9707-1	2011
Phylogeny	Lactobacillus hayakitensis sp. nov., isolated from intestines of healthy thoroughbreds.	Morita H, Shiratori C, Murakami M, Takami H, Kato Y, Endo A, Nakajima F, Takagi M, Akita H, Okada S, Masaoka T	Int J Syst Evol Microbiol	10.1099/ijs.0.65135-0	2007

References

#7791	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 18933
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#28453	IJSEM 2836 2007 ( DOI 10.1099/ijs.0.65135-0 , PubMed 18048734 )
#32211	Barberan A, Caceres Velazquez H, Jones S, Fierer N.: Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information. mSphere 2: 2017 ( DOI 10.1128/mSphere.00237-17 , PubMed 28776041 ) - originally annotated from #28453
#36198	; Curators of the CIP;
#60836	Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 56036
#66794	Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg: BRENDA, the ELIXIR core data resource in 2021: new developments and updates. Nucleic Acids Res. 49: D498 - D508 2020 ( DOI 10.1093/nar/gkaa1025 , PubMed 33211880 )
#67770	Japan Collection of Microorganism (JCM) ; Curators of the JCM;
#68382	Automatically annotated from API zym .
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#119852	Collection of Institut Pasteur ; Curators of the CIP; CIP 109885
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

Rate our new design

Content

About us

Ligilactobacillus hayakitensis (DSM 18933, CIP 109885, JCM 14209)

Name and taxonomic classification

Morphology

Cell morphology

Colony morphology

Culture and growth conditions

Culture medium

Culture temp

Physiology and metabolism

Oxygen tolerance

Spore formation

Halophily

Murein

Metabolite utilization

Metabolite tests

Enzymes

Pathway annotation

API zym

Isolation, sampling and environmental information

Isolation source categories

Isolation

Taxonmaps

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_000615845

16S sequences

GC content

Genome-based predictions

Predictions

Literature

Literature

References

BacDive

Help

Social Media