Lacticaseibacillus thailandensis (DSM 22698, BCC 21235, CIP 109884)

Name: Lacticaseibacillus thailandensis (DSM 22698, BCC 21235, CIP 109884)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 6671

Type strain

Strain Designation MCH5-2

Culture col. no. DSM 22698 BCC 21235 CIP 109884 JCM 13996 NRIC 0671 2 more

NCBI tax ID(s) 381741

Special Collections DSMZ JCM CIP

Links

Lacticaseibacillus thailandensis MCH5-2 is a microaerophile, mesophilic, Gram-positive prokaryote that was isolated from fermented tea leaves .

Gram-positive rod-shaped microaerophile mesophilic genome sequence 16S sequence

Name and taxonomic classification

SI-ID 309459

DSM 22698,BCC 21235,LMG 24278,JCM 13996,CIP 109884

@ref 20215

Last LPSN update 2025-12-16 09:48:23

Domain Bacillati

Phylum Bacillota

Class Bacilli

Order Lactobacillales

Family Lactobacillaceae

Genus Lacticaseibacillus

Species Lacticaseibacillus thailandensis

Full scientific name Lacticaseibacillus thailandensis (Tanasupawat et al. 2007) Zheng et al. 2020

Synonyms (1)

Lactobacillus thailandensis

Morphology

Cell morphology

@ref	Gram stain	Cell shape	Confidence
123493	positive	rod-shaped
125438			90
125438	positive		94.114
125439	positive		91.1

Culture and growth conditions

Culture medium

@ref	Name	Medium link	Composition
16469	MRS MEDIUM (DSMZ Medium 11)	Medium recipe at MediaDive	Name: MRS MEDIUM (DSMZ Medium 11) Composition: Glucose 20.0 g/l Casein peptone 10.0 g/l Meat extract 10.0 g/l Na-acetate 5.0 g/l Yeast extract 5.0 g/l (NH4)3 citrate 2.0 g/l K2HPO4 2.0 g/l Tween 80 1.0 g/l MgSO4 x 7 H2O 0.2 g/l MnSO4 x H2O 0.05 g/l Distilled water
33941	MEDIUM 40- for Lactobacillus and Leuconostoc		Distilled water make up to (1000.000 ml);Man Rogosa Sharp agar (68.000 g)
123493	CIP Medium 40	Medium recipe at CIP

Culture temp

@ref	Growth	Type	Temperature (°C)	Range
16469	positive	growth	37	mesophilic
33941	positive	growth	37	mesophilic
123493	positive	growth	15-37
123493	negative	growth	45

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance
16469	microaerophile
123493	facultative anaerobe

Spore formation

@ref	Spore formation	Confidence
125438		92.135

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
68371	27613 ChEBI	amygdalin	-	builds acid from	from API 50CH acid
68371	18305 ChEBI	arbutin	+	builds acid from	from API 50CH acid
68371	17057 ChEBI	cellobiose	-	builds acid from	from API 50CH acid
68371	17108 ChEBI	D-arabinose	-	builds acid from	from API 50CH acid
68371	18333 ChEBI	D-arabitol	-	builds acid from	from API 50CH acid
68371	15824 ChEBI	D-fructose	+	builds acid from	from API 50CH acid
68371	28847 ChEBI	D-fucose	-	builds acid from	from API 50CH acid
68371	17634 ChEBI	D-glucose	+	builds acid from	from API 50CH acid
68371	62318 ChEBI	D-lyxose	-	builds acid from	from API 50CH acid
68371	16899 ChEBI	D-mannitol	-	builds acid from	from API 50CH acid
68371	16024 ChEBI	D-mannose	+	builds acid from	from API 50CH acid
68371	16988 ChEBI	D-ribose	-	builds acid from	from API 50CH acid
68371	17924 ChEBI	D-sorbitol	-	builds acid from	from API 50CH acid
68371	16443 ChEBI	D-tagatose	+	builds acid from	from API 50CH acid
68371	65327 ChEBI	D-xylose	-	builds acid from	from API 50CH acid
68371	17113 ChEBI	erythritol	-	builds acid from	from API 50CH acid
68371	4853 ChEBI	esculin	+	builds acid from	from API 50CH acid
68371	16813 ChEBI	galactitol	-	builds acid from	from API 50CH acid
68371	28066 ChEBI	gentiobiose	-	builds acid from	from API 50CH acid
68371	24265 ChEBI	gluconate	-	builds acid from	from API 50CH acid
68371	17754 ChEBI	glycerol	-	builds acid from	from API 50CH acid
68371	28087 ChEBI	glycogen	-	builds acid from	from API 50CH acid
68371	15443 ChEBI	inulin	-	builds acid from	from API 50CH acid
68371	30849 ChEBI	L-arabinose	-	builds acid from	from API 50CH acid
68371	18403 ChEBI	L-arabitol	-	builds acid from	from API 50CH acid
68371	18287 ChEBI	L-fucose	-	builds acid from	from API 50CH acid
68371	62345 ChEBI	L-rhamnose	-	builds acid from	from API 50CH acid
68371	17266 ChEBI	L-sorbose	-	builds acid from	from API 50CH acid
68371	65328 ChEBI	L-xylose	-	builds acid from	from API 50CH acid
68371	17306 ChEBI	maltose	-	builds acid from	from API 50CH acid
68371	6731 ChEBI	melezitose	-	builds acid from	from API 50CH acid
68371	28053 ChEBI	melibiose	-	builds acid from	from API 50CH acid
68371	320061 ChEBI	methyl alpha-D-glucopyranoside	-	builds acid from	from API 50CH acid
68371	43943 ChEBI	methyl alpha-D-mannoside	-	builds acid from	from API 50CH acid
68371	74863 ChEBI	methyl beta-D-xylopyranoside	-	builds acid from	from API 50CH acid
68371	17268 ChEBI	myo-inositol	-	builds acid from	from API 50CH acid
68371	59640 ChEBI	N-acetylglucosamine	+	builds acid from	from API 50CH acid
123493	17632 ChEBI	nitrate	-	reduction
123493	17632 ChEBI	nitrate	+	respiration
123493	16301 ChEBI	nitrite	-	reduction
68371		Potassium 2-ketogluconate	-	builds acid from	from API 50CH acid
68371		Potassium 5-ketogluconate	-	builds acid from	from API 50CH acid
68371	16634 ChEBI	raffinose	-	builds acid from	from API 50CH acid
68371	15963 ChEBI	ribitol	-	builds acid from	from API 50CH acid
68371	17814 ChEBI	salicin	+	builds acid from	from API 50CH acid
68371	28017 ChEBI	starch	-	builds acid from	from API 50CH acid
68371	17992 ChEBI	sucrose	-	builds acid from	from API 50CH acid
68371	27082 ChEBI	trehalose	-	builds acid from	from API 50CH acid
68371	32528 ChEBI	turanose	-	builds acid from	from API 50CH acid
68371	17151 ChEBI	xylitol	-	builds acid from	from API 50CH acid

Metabolite tests

@ref	Chebi-ID	Metabolite	Voges-proskauer-test
123493	15688 ChEBI	acetoin	-

Enzymes

@ref	Value	Activity	Ec
68382	acid phosphatase	+	3.1.3.2	from API zym
123493	alcohol dehydrogenase	-	1.1.1.1
68382	alkaline phosphatase	+	3.1.3.1	from API zym
68382	alpha-chymotrypsin	-	3.4.21.1	from API zym
68382	alpha-fucosidase	-	3.2.1.51	from API zym
68382	alpha-galactosidase	-	3.2.1.22	from API zym
68382	alpha-glucosidase	-	3.2.1.20	from API zym
68382	alpha-mannosidase	-	3.2.1.24	from API zym
68382	beta-galactosidase	-	3.2.1.23	from API zym
68382	beta-glucosidase	+	3.2.1.21	from API zym
68382	beta-glucuronidase	-	3.2.1.31	from API zym
123493	catalase	-	1.11.1.6
68382	cystine arylamidase	-	3.4.11.3	from API zym
68382	esterase (C 4)	-		from API zym
68382	esterase lipase (C 8)	-		from API zym
68382	leucine arylamidase	+	3.4.11.1	from API zym
68382	lipase (C 14)	-		from API zym
123493	lysine decarboxylase	-	4.1.1.18
68382	N-acetyl-beta-glucosaminidase	-	3.2.1.52	from API zym
68382	naphthol-AS-BI-phosphohydrolase	+		from API zym
123493	ornithine decarboxylase	-	4.1.1.17
123493	oxidase	-
68382	trypsin	-	3.4.21.4	from API zym
68382	valine arylamidase	+		from API zym

Pathway annotation

Computationally determined by

@ref	pathway	enzyme coverage	annotated reactions
66794	acetoin degradation	100	3 of 3
66794	acetate fermentation	100	4 of 4
66794	formaldehyde oxidation	100	3 of 3
66794	coenzyme A metabolism	100	4 of 4
66794	palmitate biosynthesis	100	22 of 22
66794	teichoic acid biosynthesis	100	1 of 1
66794	ppGpp biosynthesis	100	4 of 4
66794	CDP-diacylglycerol biosynthesis	100	2 of 2
66794	folate polyglutamylation	100	1 of 1
66794	UDP-GlcNAc biosynthesis	100	3 of 3
66794	anapleurotic synthesis of oxalacetate	100	1 of 1
66794	cis-vaccenate biosynthesis	100	2 of 2
66794	aspartate and asparagine metabolism	88.89	8 of 9
66794	C4 and CAM-carbon fixation	87.5	7 of 8
66794	gluconeogenesis	87.5	7 of 8
66794	peptidoglycan biosynthesis	86.67	13 of 15
66794	glycolysis	76.47	13 of 17
66794	sulfopterin metabolism	75	3 of 4
66794	butanoate fermentation	75	3 of 4
66794	cardiolipin biosynthesis	71.43	5 of 7
66794	vitamin B1 metabolism	69.23	9 of 13
66794	valine metabolism	66.67	6 of 9
66794	serine metabolism	66.67	6 of 9
66794	purine metabolism	64.89	61 of 94
66794	photosynthesis	64.29	9 of 14
66794	ketogluconate metabolism	62.5	5 of 8
66794	dTDPLrhamnose biosynthesis	62.5	5 of 8
66794	pyrimidine metabolism	62.22	28 of 45
66794	phenylalanine metabolism	61.54	8 of 13
66794	NAD metabolism	61.11	11 of 18
66794	glycine betaine biosynthesis	60	3 of 5
66794	threonine metabolism	60	6 of 10
66794	methylglyoxal degradation	60	3 of 5
66794	metabolism of amino sugars and derivatives	60	3 of 5
66794	oxidative phosphorylation	58.24	53 of 91
66794	propanol degradation	57.14	4 of 7
66794	ubiquinone biosynthesis	57.14	4 of 7
66794	CO2 fixation in Crenarchaeota	55.56	5 of 9
66794	cysteine metabolism	55.56	10 of 18
66794	degradation of hexoses	55.56	10 of 18
66794	pentose phosphate pathway	54.55	6 of 11
66794	glycolate and glyoxylate degradation	50	3 of 6
66794	isoleucine metabolism	50	4 of 8
66794	ethanol fermentation	50	1 of 2
66794	methionine metabolism	50	13 of 26
66794	6-hydroxymethyl-dihydropterin diphosphate biosynthesis	50	4 of 8
66794	non-pathway related	47.37	18 of 38
66794	degradation of pentoses	46.43	13 of 28
66794	proline metabolism	45.45	5 of 11
66794	metabolism of disaccharids	45.45	5 of 11
66794	d-mannose degradation	44.44	4 of 9
66794	degradation of sugar alcohols	43.75	7 of 16
66794	glutathione metabolism	42.86	6 of 14
66794	mevalonate metabolism	42.86	3 of 7
66794	glutamate and glutamine metabolism	42.86	12 of 28
66794	lysine metabolism	40.48	17 of 42
66794	arachidonate biosynthesis	40	2 of 5
66794	Entner Doudoroff pathway	40	4 of 10
66794	glycine metabolism	40	4 of 10
66794	glycogen metabolism	40	2 of 5
66794	lipid metabolism	38.71	12 of 31
66794	urea cycle	38.46	5 of 13
66794	alanine metabolism	37.93	11 of 29
66794	d-xylose degradation	36.36	4 of 11
66794	tetrahydrofolate metabolism	35.71	5 of 14
66794	tryptophan metabolism	34.21	13 of 38
66794	selenocysteine biosynthesis	33.33	2 of 6
66794	IAA biosynthesis	33.33	1 of 3
66794	flavin biosynthesis	33.33	5 of 15
66794	acetyl CoA biosynthesis	33.33	1 of 3
66794	lipid A biosynthesis	33.33	3 of 9
66794	octane oxidation	33.33	1 of 3
66794	L-lactaldehyde degradation	33.33	1 of 3
66794	sphingosine metabolism	33.33	2 of 6
66794	androgen and estrogen metabolism	31.25	5 of 16
66794	leucine metabolism	30.77	4 of 13
66794	myo-inositol biosynthesis	30	3 of 10
66794	reductive acetyl coenzyme A pathway	28.57	2 of 7
66794	ascorbate metabolism	27.27	6 of 22
66794	isoprenoid biosynthesis	26.92	7 of 26
66794	glycogen biosynthesis	25	1 of 4
66794	lactate fermentation	25	1 of 4
66794	cyclohexanol degradation	25	1 of 4
66794	carnitine metabolism	25	2 of 8
66794	arginine metabolism	25	6 of 24
66794	degradation of aromatic, nitrogen containing compounds	25	3 of 12
66794	histidine metabolism	24.14	7 of 29
66794	sulfate reduction	23.08	3 of 13
66794	arachidonic acid metabolism	22.22	4 of 18
66794	molybdenum cofactor biosynthesis	22.22	2 of 9
66794	nitrate assimilation	22.22	2 of 9
66794	chorismate metabolism	22.22	2 of 9
66794	polyamine pathway	21.74	5 of 23
66794	tyrosine metabolism	21.43	3 of 14
66794	citric acid cycle	21.43	3 of 14

API zym

@ref	Control	Alkaline phosphatase	Esterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	Lipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	Trypsin	alpha- Chymotrypsin	Acid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	alpha- Galactosidase	beta- Galactosidase	beta- Glucuronidase	alpha- Glucosidase	beta- Glucosidase	N-acetyl-beta- glucosaminidase	alpha- Mannosidase	alpha- Fucosidase
123493	-	+	-	-	-	+	+	-	-	-	+	+	-	-	-	-	+	-	-	-

API 50CHac

@ref	ControlQ	GLY	ERY	DARA	LARA	RIB	DXYL	LXYL	ADO	MDX	GAL	GLU	FRU	MNE	SBE	RHA	DUL	INO	MAN	SOR	MDM	MDG	NAG	AMY	ARB	ESC	SAL	CEL	MAL	LAC	MEL	SAC	TRE	INU	MLZ	RAF	AMD	GLYG	XLT	GEN	TUR	LYX	TAG	DFUC	LFUC	DARL	LARL	GNT	2KG	5KG
16469	-	-	-	-	-	-	-	-	-	-	+	+	+	+	-	-	-	-	-	-	-	-	+	-	+	+	+	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	+	-	-	-	-	-	-	-
123493	not determinedn.d.	-	-	-	-	-	-	-	-	-	+/-	+	+	+	-	-	-	-	-	-	-	-	+	-	+	+	+	-	-	+/-	-	-	-	-	-	-	-	-	-	-	-	-	+	-	-	-	-	-	-	-

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Engineered	#Food production	-
#Host Body-Site	#Plant	#Leaf (Phyllosphere)

Isolation

@ref	Sample type	Geographic location	Country	Country ISO 3 Code	Continent	Isolation date
16469	fermented tea leaves (miang)	Chiang Mai, Varoroch market	Thailand	THA	Asia
123493	Plant, Fermented tea leaves	Bangkok	Thailand	THA	Asia	2007

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
16469	1	Risk group (German classification) According to TRBA 466
123493	1	Risk group (French classification) According to TRBA 466

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
66792	ASM143613v1 assembly for Lacticaseibacillus thailandensis DSM 22698 = JCM 13996	scaffold	GCA_001436135	1423810.4	2693429543	1423810	73.21
66792	ASM131286v1 assembly for Lacticaseibacillus thailandensis DSM 22698 = JCM 13996	contig	GCA_001312865	1423810.3	2734481919	1423810	48.64

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
16469	Lactobacillus thailandensis gene for 16S rRNA, complete sequence	AB257863	1535		381741

GC content

@ref	GC-content (mol%)	Method
16469	49.0	high performance liquid chromatography (HPLC)

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: Lacticaseibacillus thailandensis DSM 22698 = JCM 13996
NCBI: GCA_001436135

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	spore_formation	BacteriaNetⓘ	no	73.80	no
125439	motility	BacteriaNetⓘ	yes	52.10	no
125439	gram_stain	BacteriaNetⓘ	positive	91.10	no
125439	oxygen_tolerance	BacteriaNetⓘ	anaerobe	88.10	no

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Lacticaseibacillus thailandensis DSM 22698 = JCM 13996 DSM 22698
NCBI: GCA_001436135.1

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	yes	94.11	no
125438	anaerobic	anaerobicⓘ	no	83.88	yes
125438	aerobic	aerobicⓘ	no	96.02	no
125438	spore-forming	spore-formingⓘ	no	92.14	no
125438	thermophilic	thermophileⓘ	no	96.50	yes
125438	flagellated	motile2+ⓘ	no	90.00	no

Literature

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Identification of lactic acid bacteria from fermented tea leaves (miang) in Thailand and proposals of Lactobacillus thailandensis sp. nov., Lactobacillus camelliae sp. nov., and Pediococcus siamensis sp. nov.	Tanasupawat S, Pakdeeto A, Thawai C, Yukphan P, Okada S	J Gen Appl Microbiol	10.2323/jgam.53.7	2007

References

#16469	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 22698
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#33941	; Curators of the CIP;
#66792	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .
#66794	Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg: BRENDA, the ELIXIR core data resource in 2021: new developments and updates. Nucleic Acids Res. 49: D498 - D508 2020 ( DOI 10.1093/nar/gkaa1025 , PubMed 33211880 )
#68371	Automatically annotated from API 50CH acid .
#68382	Automatically annotated from API zym .
#123493	Collection of Institut Pasteur ; Curators of the CIP; CIP 109884
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Lacticaseibacillus thailandensis (DSM 22698, BCC 21235, CIP 109884)

Name and taxonomic classification

Morphology

Cell morphology

Culture and growth conditions

Culture medium

Culture temp

Physiology and metabolism

Oxygen tolerance

Spore formation

Metabolite utilization

Metabolite tests

Enzymes

Pathway annotation

API zym

API 50CHac

Isolation, sampling and environmental information

Isolation source categories

Isolation

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_001436135

16S sequences

GC content

Genome-based predictions

Predictions

Literature

Literature

References

BacDive

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