Strain identifier

BacDive ID: 6671

Type strain: Yes

Species: Lacticaseibacillus thailandensis

Strain Designation: MCH5-2

Strain history: CIP <- 2008, JCM <- 2006, S. Tanasupawat MCH5-2, Bangkok Univ, Thailand: strain 13996

NCBI tax ID(s): 381741 (species)

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General

@ref: 16469

BacDive-ID: 6671

DSM-Number: 22698

keywords: genome sequence, 16S sequence, Bacteria, microaerophile, mesophilic

description: Lacticaseibacillus thailandensis MCH5-2 is a microaerophile, mesophilic bacterium that was isolated from fermented tea leaves .

NCBI tax id

  • NCBI tax id: 381741
  • Matching level: species

strain history

@refhistory
16469<- CIP <- S. Tanasupawat, Dept. Microbiology, Chulalongkorn Univ., Thailand; MCH5-2
123493CIP <- 2008, JCM <- 2006, S. Tanasupawat MCH5-2, Bangkok Univ, Thailand: strain 13996

doi: 10.13145/bacdive6671.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Lactobacillales
  • family: Lactobacillaceae
  • genus: Lacticaseibacillus
  • species: Lacticaseibacillus thailandensis
  • full scientific name: Lacticaseibacillus thailandensis (Tanasupawat et al. 2007) Zheng et al. 2020

  • synonyms

    • @ref: 20215
    • synonym: Lactobacillus thailandensis

@ref: 16469

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Lactobacillales

family: Lactobacillaceae

genus: Lacticaseibacillus

species: Lacticaseibacillus thailandensis

full scientific name: Lacticaseibacillus thailandensis (Tanasupawat et al. 2007) Zheng et al. 2020

strain designation: MCH5-2

type strain: yes

Morphology

cell morphology

@refmotilityconfidencegram staincell shape
69480no96.282
69480100positive
123493nopositiverod-shaped

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
16469MRS MEDIUM (DSMZ Medium 11)yeshttps://mediadive.dsmz.de/medium/11Name: MRS MEDIUM (DSMZ Medium 11) Composition: Glucose 20.0 g/l Casein peptone 10.0 g/l Meat extract 10.0 g/l Na-acetate 5.0 g/l Yeast extract 5.0 g/l (NH4)3 citrate 2.0 g/l K2HPO4 2.0 g/l Tween 80 1.0 g/l MgSO4 x 7 H2O 0.2 g/l MnSO4 x H2O 0.05 g/l Distilled water
33941MEDIUM 40- for Lactobacillus and LeuconostocyesDistilled water make up to (1000.000 ml);Man Rogosa Sharp agar (68.000 g)
123493CIP Medium 40yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=40

culture temp

@refgrowthtypetemperaturerange
16469positivegrowth37mesophilic
33941positivegrowth37mesophilic
123493positivegrowth15-37
123493nogrowth45thermophilic

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
16469microaerophile
123493facultative anaerobe

spore formation

@refspore formationconfidence
69481no100
69480no99.993

metabolite utilization

@refmetaboliteutilization activitykind of utilization testedChebi-ID
68371Potassium 5-ketogluconate-builds acid from
68371Potassium 2-ketogluconate-builds acid from
68371gluconate-builds acid from24265
68371L-arabitol-builds acid from18403
68371D-arabitol-builds acid from18333
68371L-fucose-builds acid from18287
68371D-fucose-builds acid from28847
68371D-tagatose+builds acid from16443
68371D-lyxose-builds acid from62318
68371turanose-builds acid from32528
68371gentiobiose-builds acid from28066
68371xylitol-builds acid from17151
68371glycogen-builds acid from28087
68371starch-builds acid from28017
68371raffinose-builds acid from16634
68371melezitose-builds acid from6731
68371inulin-builds acid from15443
68371trehalose-builds acid from27082
68371sucrose-builds acid from17992
68371melibiose-builds acid from28053
68371maltose-builds acid from17306
68371cellobiose-builds acid from17057
68371salicin+builds acid from17814
68371esculin+builds acid from4853
68371arbutin+builds acid from18305
68371amygdalin-builds acid from27613
68371N-acetylglucosamine+builds acid from59640
68371methyl alpha-D-glucopyranoside-builds acid from320061
68371methyl alpha-D-mannoside-builds acid from43943
68371D-sorbitol-builds acid from17924
68371D-mannitol-builds acid from16899
68371myo-inositol-builds acid from17268
68371galactitol-builds acid from16813
68371L-rhamnose-builds acid from62345
68371L-sorbose-builds acid from17266
68371D-mannose+builds acid from16024
68371D-fructose+builds acid from15824
68371D-glucose+builds acid from17634
68371methyl beta-D-xylopyranoside-builds acid from74863
68371ribitol-builds acid from15963
68371L-xylose-builds acid from65328
68371D-xylose-builds acid from65327
68371D-ribose-builds acid from16988
68371L-arabinose-builds acid from30849
68371D-arabinose-builds acid from17108
68371erythritol-builds acid from17113
68371glycerol-builds acid from17754
123493nitrate-reduction17632
123493nitrite-reduction16301
123493nitrate+respiration17632

metabolite tests

  • @ref: 123493
  • Chebi-ID: 15688
  • metabolite: acetoin
  • voges-proskauer-test: -

enzymes

@refvalueactivityec
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382beta-glucosidase+3.2.1.21
68382alpha-glucosidase-3.2.1.20
68382beta-glucuronidase-3.2.1.31
68382beta-galactosidase-3.2.1.23
68382alpha-galactosidase-3.2.1.22
68382naphthol-AS-BI-phosphohydrolase+
68382acid phosphatase+3.1.3.2
68382alpha-chymotrypsin-3.4.21.1
68382trypsin-3.4.21.4
68382cystine arylamidase-3.4.11.3
68382valine arylamidase+
68382leucine arylamidase+3.4.11.1
68382lipase (C 14)-
68382esterase lipase (C 8)-
68382esterase (C 4)-
68382alkaline phosphatase+3.1.3.1
123493oxidase-
123493alcohol dehydrogenase-1.1.1.1
123493catalase-1.11.1.6
123493lysine decarboxylase-4.1.1.18
123493ornithine decarboxylase-4.1.1.17

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
123493-+---++---++----+---

API 50CHac

@refQGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
16469----------++++--------+-+++---------------+-------
123493---------+/-+++--------+-+++--+/-------------+-------

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentisolation date
16469fermented tea leaves (miang)Chiang Mai, Varoroch marketThailandTHAAsia
123493Plant, Fermented tea leavesBangkokThailandTHAAsia2007

isolation source categories

Cat1Cat2Cat3
#Engineered#Food production
#Host Body-Site#Plant#Leaf (Phyllosphere)

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
164691Risk group (German classification)
1234931Risk group (French classification)

Sequence information

16S sequences

  • @ref: 16469
  • description: Lactobacillus thailandensis gene for 16S rRNA, complete sequence
  • accession: AB257863
  • length: 1535
  • database: ena
  • NCBI tax ID: 381741

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Lacticaseibacillus thailandensis DSM 22698 = JCM 13996GCA_001436135scaffoldncbi1423810
66792Lacticaseibacillus thailandensis DSM 22698 = JCM 13996GCA_001312865contigncbi1423810
66792Lactobacillus thailandensis DSM 22698 = JCM 139961423810.4wgspatric1423810
66792Lactobacillus thailandensis DSM 22698 = JCM 139961423810.3wgspatric1423810
66792Lacticaseibacillus thailandensis JCM 139962734481919draftimg1423810
66792Lacticaseibacillus thailandensis DSM 226982693429543draftimg1423810

GC content

  • @ref: 16469
  • GC-content: 49.0
  • method: high performance liquid chromatography (HPLC)

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingno100no
flagellatedno98.182no
gram-positiveyes95.662no
anaerobicno89.101yes
halophileyes89.164no
spore-formingno96.804no
glucose-utilyes90.893no
motileno95.034no
aerobicno97.004yes
thermophileno99.353no
glucose-fermentyes89.285no

External links

@ref: 16469

culture collection no.: DSM 22698, BCC 21235, CIP 109884, JCM 13996, NRIC 0671, PCU 272

straininfo link

  • @ref: 76145
  • straininfo: 309459

literature

  • topic: Phylogeny
  • Pubmed-ID: 17429157
  • title: Identification of lactic acid bacteria from fermented tea leaves (miang) in Thailand and proposals of Lactobacillus thailandensis sp. nov., Lactobacillus camelliae sp. nov., and Pediococcus siamensis sp. nov.
  • authors: Tanasupawat S, Pakdeeto A, Thawai C, Yukphan P, Okada S
  • journal: J Gen Appl Microbiol
  • DOI: 10.2323/jgam.53.7
  • year: 2007
  • mesh: Base Composition, *Fermentation, Lactobacillus/classification/genetics/*isolation & purification/metabolism, Plant Leaves/*microbiology, RNA, Ribosomal, 16S/genetics, Tea/*microbiology
  • topic2: Enzymology

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
16469Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 22698)https://www.dsmz.de/collection/catalogue/details/culture/DSM-22698
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
33941Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/7677
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
66794Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg10.1093/nar/gkaa1025BRENDA, the ELIXIR core data resource in 2021: new developments and updatesNucleic Acids Res. 49: D498-D508 202033211880
68371Automatically annotated from API 50CH acid
68382Automatically annotated from API zym
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
76145Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID309459.1StrainInfo: A central database for resolving microbial strain identifiers
123493Curators of the CIPCollection of Institut Pasteur (CIP 109884)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20109884