Name: Limosilactobacillus oris 5A1
Creator: BacDive
License: https://creativecommons.org/licenses/by/4.0/

Strain identifier

BacDive ID: 6486

Type strain:

Species: Limosilactobacillus oris

Strain Designation: 5A1

Culture col. no.: DSM 4864, ATCC 49062, CIP 105162, NCDO 2160, NCIB 8831, CCUG 37396, JCM 11028, CECT 4021, CIP 103255, JCM 7507, KCTC 3670, LMG 9848, NCIMB 8831

Strain history: CIP <- 1997, DSM <- NCDO <- A.C. Hayward: strain 5A1, Lactobacillus brevis

NCBI tax ID(s): 1423779 (strain), 1632 (species)

SI-ID 3328

LMG 9848, ATCC 49062, DSM 4864, NCFB 2160, NCIMB 8831, NCDO 2160, NCIB 8831, CCUG 37396, CIP 105162, JCM 11028, JCM 7507, CIP103255, CECT 4021, CCRC 14762, NCFB 1440, NCFB 2351, KCTC 3502, KCTC 3670, CIP 14020, BCRC 14762

Special Collections

Other strains from Limosilactobacillus oris

Section

Limosilactobacillus oris 5A1 is an aerobe, Gram-positive bacterium that was isolated from human saliva.

Gram-positive
aerobe
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Bacillota*
	Class	*Bacilli*
	Order	*Lactobacillales*
	Family	*Lactobacillaceae*
	Genus	*Limosilactobacillus*
	Species	**Limosilactobacillus oris**
	Full Scientific Name (LPSN)	Limosilactobacillus oris (Farrow and Collins 1988) Zheng et al. 2020

Synonym

Lactobacillus oris

Information on morphological and physiological properties Morphology

[Ref.: #121443]	Gram stain	positive
[Ref.: #69480]	Gram stain	positive Confidence in %95.408

[Ref.: #121443]	Cell shape	rod-shaped

[Ref.: #121443]	Motility	no
[Ref.: #69480]	Motility	no Confidence in %93.5

Information on culture and growth conditions Culture and growth conditions

[Ref.: #1854]

Culture medium

MRS MEDIUM (DSMZ Medium 11)

[Ref.: #1854]

Culture medium growth

[Ref.: #1854]

Culture medium link

Medium recipe at MediaDive

[Ref.: #1854]

Culture medium composition

expand / minimize

[Ref.: #34712]

Culture medium

MEDIUM 40- for Lactobacillus and Leuconostoc

[Ref.: #34712]

Culture medium growth

[Ref.: #34712]

Culture medium composition

expand / minimize

[Ref.: #121443]

Culture medium

CIP Medium 40

[Ref.: #121443]

Culture medium growth

[Ref.: #121443]

Culture medium link

Medium recipe provided by DSMZ

	Kind of temperature		Temperature
[Ref.: #1854]		growth	37 °C
[Ref.: #34712]		growth	37 °C
[Ref.: #53648]		growth	37 °C
[Ref.: #67770]		growth	30 °C
[Ref.: #121443]		growth	30-41 °C
[Ref.: #121443]	no	growth	10 °C
[Ref.: #121443]	no	growth	15 °C
[Ref.: #121443]	no	growth	22 °C
[Ref.: #121443]	no	growth	45 °C

Information on physiology and metabolism Physiology and metabolism

[Ref.: #53648]	Oxygen tolerance	aerobe
[Ref.: #53648]	Oxygen tolerance	anaerobe
[Ref.: #121443]	Oxygen tolerance	facultative anaerobe

[Ref.: #69480]	Ability of spore formation	no Confidence in %92.175
[Ref.: #69481]	Ability of spore formation	no Confidence in %100

[Ref.: #1854]	Murein short key	A11.31
[Ref.: #1854]	Murein types	A4alpha L-Lys-D-Asp

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #68371]	amygdalin ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	arbutin ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	cellobiose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-arabinose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-fructose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-fucose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-galactose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-glucose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-lyxose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-mannitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-mannose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-ribose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-sorbitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-tagatose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-xylose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	erythritol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	galactitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	gentiobiose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	glycerol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	glycogen ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	inulin ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-arabinose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-arabitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-fucose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-rhamnose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-sorbose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-xylose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	maltose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	melezitose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	melibiose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	methyl alpha-D-mannoside ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	myo-inositol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #121443]	nitrate ChEBI	-	reduction
[Ref.: #121443]	nitrate ChEBI	+	respiration
[Ref.: #121443]	nitrite ChEBI	-	reduction
[Ref.: #68371]	Potassium 2-ketogluconate ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	Potassium 5-ketogluconate ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	raffinose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	ribitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	starch ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	sucrose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	trehalose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	xylitol ChEBI	-	builds acid from	* from API 50CH acid
	Only first 10 entries are displayed. Click here to see all.

	Physiological tests	Metabolite	Voges-Proskauer-test
[Ref.: #121443]		acetoin ChEBI	-

	Enzyme	Enzyme activity	EC number
[Ref.: #68382]	acid phosphatase	+	3.1.3.2	* from API zym
[Ref.: #121443]	alcohol dehydrogenase	-	1.1.1.1
[Ref.: #68382]	alkaline phosphatase	+	3.1.3.1	* from API zym
[Ref.: #68382]	alpha-chymotrypsin	-	3.4.21.1	* from API zym
[Ref.: #68382]	alpha-fucosidase	-	3.2.1.51	* from API zym
[Ref.: #68382]	alpha-galactosidase	+	3.2.1.22	* from API zym
[Ref.: #68382]	alpha-glucosidase	+	3.2.1.20	* from API zym
[Ref.: #68382]	alpha-mannosidase	-	3.2.1.24	* from API zym
[Ref.: #68382]	beta-galactosidase	+	3.2.1.23	* from API zym
[Ref.: #68382]	beta-glucosidase	+	3.2.1.21	* from API zym
[Ref.: #68382]	beta-glucuronidase	-	3.2.1.31	* from API zym
[Ref.: #121443]	catalase	-	1.11.1.6
[Ref.: #68382]	cystine arylamidase	-	3.4.11.3	* from API zym
[Ref.: #68382]	esterase (C 4)	+		* from API zym
[Ref.: #68382]	esterase lipase (C 8)	+		* from API zym
[Ref.: #68382]	leucine arylamidase	+	3.4.11.1	* from API zym
[Ref.: #68382]	lipase (C 14)	-		* from API zym
[Ref.: #121443]	lysine decarboxylase	-	4.1.1.18
[Ref.: #68382]	N-acetyl-beta-glucosaminidase	-	3.2.1.52	* from API zym
[Ref.: #68382]	naphthol-AS-BI-phosphohydrolase	+		* from API zym
[Ref.: #121443]	ornithine decarboxylase	+	4.1.1.17
[Ref.: #121443]	oxidase	-
[Ref.: #68382]	trypsin	-	3.4.21.4	* from API zym
[Ref.: #68382]	valine arylamidase	-		* from API zym
	Only first 10 entries are displayed. Click here to see all.

API® 50CH acid:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	ControlQ	Acid from glycerolGLY	Acid from erythritolERY	Acid from D-arabinoseDARA	Acid from L-arabinoseLARA	Acid from D-riboseRIB	Acid from D-xyloseDXYL	Acid from L-xyloseLXYL	Acid from D-adonitolADO	Acid from methyl beta-D-xylopyranosideMDX	Acid from D-galactoseGAL	Acid from D-glucoseGLU	Acid from D-fructoseFRU	Acid from D-mannoseMNE	Acid from L-sorboseSBE	Acid from L-rhamnoseRHA	Acid from dulcitolDUL	Acid from inositolINO	Acid from D-mannitolMAN	Acid from D-sorbitolSOR	Acid from methyl alpha-D-mannopyranosideMDM	Acid from methyl alpha-D-glucopyranosideMDG	Acid from N-acetyl-glucosamineNAG	Acid from amygdalinAMY	Acid from arbutinARB	Acid from esculinESC	Acid from salicinSAL	Acid from cellobioseCEL	Acid from maltoseMAL	Acid from lactoseLAC	Acid from melibioseMEL	Acid from sucroseSAC	Acid from trehaloseTRE	Acid from inulinINU	Acid from melezitoseMLZ	Acid from raffinoseRAF	Acid from starchAMD	Acid from glycogenGLYG	Acid from xylitolXLT	Acid from gentiobioseGEN	Acid from turanoseTUR	Acid from D-lyxoseLYX	Acid from D-tagatoseTAG	Acid from D-fucoseDFUC	Acid from L-fucoseLFUC	Acid from D-arabitolDARL	Acid from L-arabitolLARL	Acid from gluconateGNT	Acid from 2-Ketogluconate2KG	Acid from 5-Ketogluconate5KG
[Ref.: #1854]	14223	-	-	-	-	+	+	+	-	-	-	+	+	+	-	-	-	-	-	-	-	-	-	-	+	-	+	-	-	+	-	+	+	-	-	-	+	-	-	-	+	-	-	-	-	-	+	-	+	-	-
[Ref.: #121443]	9965	not determinedn.d.	-	-	-	+	+	+	-	-	+/-	+	+	+	-	-	-	-	-	-	-	-	+/-	+/-	+	-	+/-	+/-	-	+	+/-	+	+	-	-	-	+	-	-	-	+	+/-	-	-	-	-	+/-	-	+/-	-	-

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #121443]	8498	-	+	+	+	-	+	-	-	-	-	+	+	+	+	-	+	+	-	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #1854]	Sample type/isolated from	human saliva

[Ref.: #53648]	Sample type/isolated from	Human saliva

[Ref.: #67770]	Sample type/isolated from	Human saliva

[Ref.: #121443]	Sample type/isolated from	Human, Saliva
* marker position based on {}

Isolation sources categories

#Host	#Human	-
#Host Body Product	#Oral cavity and Airways	#Saliva

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence X94229 (>99% sequence identity) for Limosilactobacillus oris from Microbeatlas

Aquatic Samples	630
Soil Samples	440
Animal Samples	32291
Plant Samples	233
Total Samples	33594

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #1854]	Biosafety level	1	Risk group (German classification) According to TRBA 466
[Ref.: #121443]	Biosafety level	1	Risk group (French classification)

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #1854]	L.oris 16S ribosomal RNA	X94229	1512	GenBank ENA	1632 tax ID
[Ref.: #67770]	Lactobacillus oris gene for 16S ribosomal RNA, partial sequence, strain: JCM 11028	LC097079	1511	GenBank ENA	1632 tax ID
[Ref.: #20218]	Lactobacillus oris strain DSM 4864 16S-23S ribosomal RNA intergenic spacer and 23S ribosomal RNA gene, partial sequence	EU161599	792	GenBank ENA	1632 tax ID	*
[Ref.: #124043]	Lactobacillus oris gene for 16S rRNA, partial sequence, strain: JCM 11028.	AB289207	645	GenBank ENA	1632 tax ID	*
[Ref.: #20218]	L.oris 16S rRNA gene	X61131	1359	GenBank ENA	1632 tax ID	*

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #67770]	Limosilactobacillus oris DSM 4864	GCA_001434465	contig	GenBank ENA	1423779 tax ID
[Ref.: #66792]	Lactobacillus oris DSM 4864	1423779.3	wgs	PATRIC	1423779 tax ID	*
[Ref.: #66792]	Limosilactobacillus oris DSM 4864	2663763371	draft	JGI IMG/M	1423779 tax ID	*

[Ref.: #1854]	GC-content	49.3 mol%
[Ref.: #67770]	GC-content	50 mol%	genome sequence analysis
[Ref.: #67770]	GC-content	49.3 mol%	thermal denaturation, midpoint method (Tm)

Data predicted using genome information as a basis Genome-based predictions

	Trait	Model	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Lactobacillus oris DSM 4864 PATRIC: 1423779.3
[Ref.: #69481]	spore-forming	spore-formingⓘ	no	100	no

	Trait	Model	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Lactobacillus oris DSM 4864 PATRIC: 1423779.3
[Ref.: #69480]	gram-positive	gram-positiveⓘ	yes	95.408	no
[Ref.: #69480]	anaerobic	anaerobicⓘ	no	69.298	no
[Ref.: #69480]	spore-forming	spore-formingⓘ	no	92.175	no
[Ref.: #69480]	aerobic	aerobicⓘ	no	93.319	no
[Ref.: #69480]	thermophilic	thermophileⓘ	no	97	yes
[Ref.: #69480]	flagellated	motile2+ⓘ	no	93.5	no

Availability in culture collections External links

[Ref.: #1854]

Culture collection no.

DSM 4864, ATCC 49062, CIP 105162, NCDO 2160, NCIB 8831, CCUG 37396, JCM 11028, CECT 4021, CIP 103255, JCM 7507, KCTC 3670, LMG 9848, NCIMB 8831

[Ref.: #75959]

SI-ID 3328

References

#1854

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 4864

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#20218

Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )

#34712 ; Curators of the CIP;
#53648 Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 37396

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#68371

Automatically annotated from API 50CH acid .

#68382

Automatically annotated from API zym .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#75959

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID3328.1 )

#121443 Collection of Institut Pasteur ; Curators of the CIP; CIP 105162

#124043

Dr. Isabel Schober, Dr. Julia Koblitz: Data extracted from sequence databases, automatically matched based on designation and taxonomy .

* These data were automatically processed and therefore are not curated

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Lactobacillus oris DSM 4864

Lactobacillus oris DSM 4864

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