Limosilactobacillus oris (DSM 4864, ATCC 49062, CIP 105162)

Name: Limosilactobacillus oris (DSM 4864, ATCC 49062, CIP 105162)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 6486

Type strain

Strain Designation 5A1

Culture col. no. DSM 4864 ATCC 49062 CIP 105162 NCDO 2160 NCIB 8831 9 more

NCBI tax ID(s) 1423779 1632

Special Collections DSMZ CCUG JCM KCTC CIP

History DSMZ <- NCDO <- A.C. Hayward, 5A1 JCM <-- CIP 105162 <-- DSM 4864 <-- NCDO 2160 <-- A. C. Hayward 5A1. CIP <- 1997, DSM <- NCDO <- A.C. Hayward: strain 5A1, Lactobacillus brevis

Links

Limosilactobacillus oris 5A1 is an aerobe, Gram-positive, rod-shaped bacterium that was isolated from human saliva.

Gram-positive rod-shaped aerobe genome sequence 16S sequence Bacteria

Name and taxonomic classification

SI-ID 3328

LMG 9848,ATCC 49062,DSM 4864,NCFB 2160,NCIMB 8831,NCDO 2160,NCIB 8831,CCUG 37396,CIP 105162,JCM 11028,JCM 7507,CIP103255,CECT 4021,CCRC 14762,NCFB 1440,NCFB 2351,KCTC 3502,KCTC 3670,CIP 14020,BCRC 14762

@ref 20215

Last LPSN update 2026-05-29 08:26:25

Domain Bacteria

Phylum Bacillota

Class Bacilli

Order Lactobacillales

Family Lactobacillaceae

Genus Limosilactobacillus

Species Limosilactobacillus oris

Full scientific name Limosilactobacillus oris (Farrow and Collins 1988) Zheng et al. 2020

Synonyms (1)

Lactobacillus oris

BacDive ID	Other strains from **Limosilactobacillus oris** (4)	Type strain
6487	L. oris 5B1, DSM 4865, NCDO 2161
6488	L. oris 5G, DSM 4866, NCDO 2163
160079	L. oris Cla-CZ-99, DSM 108973
173347	L. oris CRBIP24.12

Morphology

Cell morphology

@ref	Gram stain	Cell shape	Confidence
121443	positive	rod-shaped
125438			93.5
125438	positive		95.408

Culture and growth conditions

Culture medium

@ref	Name	Medium link	Composition
1854	MRS MEDIUM (DSMZ Medium 11)	Medium recipe at MediaDive	Name: MRS MEDIUM (DSMZ Medium 11) Composition: Glucose 20.0 g/l Casein peptone 10.0 g/l Meat extract 10.0 g/l Na-acetate 5.0 g/l Yeast extract 5.0 g/l (NH4)3 citrate 2.0 g/l K2HPO4 2.0 g/l Tween 80 1.0 g/l MgSO4 x 7 H2O 0.2 g/l MnSO4 x H2O 0.05 g/l Distilled water
34712	MEDIUM 40- for Lactobacillus and Leuconostoc		Distilled water make up to (1000.000 ml);Man Rogosa Sharp agar (68.000 g)
121443	CIP Medium 40	Medium recipe at CIP

Culture temp

@ref	Growth	Type	Temperature (°C)
1854	positive	growth	37
34712	positive	growth	37
53648	positive	growth	37
67770	positive	growth	30
121443	positive	growth	30-41
121443	negative	growth	10
121443	negative	growth	15
121443	negative	growth	22
121443	negative	growth	45

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance
53648	aerobe
53648	anaerobe
121443	facultative anaerobe

Spore formation

@ref	Spore formation	Confidence
125438		92.175

Murein

@ref	Murein short key	Type
1854	A11.31	A4alpha L-Lys-D-Asp

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
68371	27613 ChEBI	amygdalin	+	builds acid from	from API 50CH acid
68371	18305 ChEBI	arbutin	-	builds acid from	from API 50CH acid
68371	17057 ChEBI	cellobiose	-	builds acid from	from API 50CH acid
68371	17108 ChEBI	D-arabinose	-	builds acid from	from API 50CH acid
68371	15824 ChEBI	D-fructose	+	builds acid from	from API 50CH acid
68371	28847 ChEBI	D-fucose	-	builds acid from	from API 50CH acid
68371	12936 ChEBI	D-galactose	+	builds acid from	from API 50CH acid
68371	17634 ChEBI	D-glucose	+	builds acid from	from API 50CH acid
68371	62318 ChEBI	D-lyxose	-	builds acid from	from API 50CH acid
68371	16899 ChEBI	D-mannitol	-	builds acid from	from API 50CH acid
68371	16024 ChEBI	D-mannose	-	builds acid from	from API 50CH acid
68371	16988 ChEBI	D-ribose	+	builds acid from	from API 50CH acid
68371	17924 ChEBI	D-sorbitol	-	builds acid from	from API 50CH acid
68371	16443 ChEBI	D-tagatose	-	builds acid from	from API 50CH acid
68371	65327 ChEBI	D-xylose	+	builds acid from	from API 50CH acid
68371	17113 ChEBI	erythritol	-	builds acid from	from API 50CH acid
68371	16813 ChEBI	galactitol	-	builds acid from	from API 50CH acid
68371	28066 ChEBI	gentiobiose	+	builds acid from	from API 50CH acid
68371	17754 ChEBI	glycerol	-	builds acid from	from API 50CH acid
68371	28087 ChEBI	glycogen	-	builds acid from	from API 50CH acid
68371	15443 ChEBI	inulin	-	builds acid from	from API 50CH acid
68371	30849 ChEBI	L-arabinose	+	builds acid from	from API 50CH acid
68371	18403 ChEBI	L-arabitol	-	builds acid from	from API 50CH acid
68371	18287 ChEBI	L-fucose	-	builds acid from	from API 50CH acid
68371	62345 ChEBI	L-rhamnose	-	builds acid from	from API 50CH acid
68371	17266 ChEBI	L-sorbose	-	builds acid from	from API 50CH acid
68371	65328 ChEBI	L-xylose	-	builds acid from	from API 50CH acid
68371	17306 ChEBI	maltose	+	builds acid from	from API 50CH acid
68371	6731 ChEBI	melezitose	-	builds acid from	from API 50CH acid
68371	28053 ChEBI	melibiose	+	builds acid from	from API 50CH acid
68371	43943 ChEBI	methyl alpha-D-mannoside	-	builds acid from	from API 50CH acid
68371	17268 ChEBI	myo-inositol	-	builds acid from	from API 50CH acid
121443	17632 ChEBI	nitrate	-	reduction
121443	17632 ChEBI	nitrate	+	respiration
121443	16301 ChEBI	nitrite	-	reduction
68371	0 ChEBI	Potassium 2-ketogluconate	-	builds acid from	from API 50CH acid
68371	0 ChEBI	Potassium 5-ketogluconate	-	builds acid from	from API 50CH acid
68371	16634 ChEBI	raffinose	+	builds acid from	from API 50CH acid
68371	15963 ChEBI	ribitol	-	builds acid from	from API 50CH acid
68371	28017 ChEBI	starch	-	builds acid from	from API 50CH acid
68371	17992 ChEBI	sucrose	+	builds acid from	from API 50CH acid
68371	27082 ChEBI	trehalose	-	builds acid from	from API 50CH acid
68371	17151 ChEBI	xylitol	-	builds acid from	from API 50CH acid

Metabolite tests

@ref	Chebi-ID	Metabolite	Voges-proskauer-test
121443	15688 ChEBI	acetoin	-

Enzymes

@ref	Value	Activity	Ec
68382	acid phosphatase	+	3.1.3.2	from API zym
121443	alcohol dehydrogenase	-	1.1.1.1
68382	alkaline phosphatase	+	3.1.3.1	from API zym
68382	alpha-chymotrypsin	-	3.4.21.1	from API zym
68382	alpha-fucosidase	-	3.2.1.51	from API zym
68382	alpha-galactosidase	+	3.2.1.22	from API zym
68382	alpha-glucosidase	+	3.2.1.20	from API zym
68382	alpha-mannosidase	-	3.2.1.24	from API zym
68382	beta-galactosidase	+	3.2.1.23	from API zym
68382	beta-glucosidase	+	3.2.1.21	from API zym
68382	beta-glucuronidase	-	3.2.1.31	from API zym
121443	catalase	-	1.11.1.6
68382	cystine arylamidase	-	3.4.11.3	from API zym
68382	esterase (C 4)	+		from API zym
68382	esterase lipase (C 8)	+		from API zym
68382	leucine arylamidase	+	3.4.11.1	from API zym
68382	lipase (C 14)	-		from API zym
121443	lysine decarboxylase	-	4.1.1.18
68382	N-acetyl-beta-glucosaminidase	-	3.2.1.52	from API zym
68382	naphthol-AS-BI-phosphohydrolase	+		from API zym
121443	ornithine decarboxylase	+	4.1.1.17
121443	oxidase	-
68382	trypsin	-	3.4.21.4	from API zym
68382	valine arylamidase	-		from API zym

API zym

@ref	Control	Alkaline phosphatase	Esterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	Lipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	Trypsin	alpha- Chymotrypsin	Acid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	alpha- Galactosidase	beta- Galactosidase	beta- Glucuronidase	alpha- Glucosidase	beta- Glucosidase	N-acetyl-beta- glucosaminidase	alpha- Mannosidase	alpha- Fucosidase
121443	-	+	+	+	-	+	-	-	-	-	+	+	+	+	-	+	+	-	-	-

API 50CHac

@ref	ControlQ	GLY	ERY	DARA	LARA	RIB	DXYL	LXYL	ADO	MDX	GAL	GLU	FRU	MNE	SBE	RHA	DUL	INO	MAN	SOR	MDM	MDG	NAG	AMY	ARB	ESC	SAL	CEL	MAL	LAC	MEL	SAC	TRE	INU	MLZ	RAF	AMD	GLYG	XLT	GEN	TUR	LYX	TAG	DFUC	LFUC	DARL	LARL	GNT	2KG	5KG
1854	-	-	-	-	+	+	+	-	-	-	+	+	+	-	-	-	-	-	-	-	-	-	-	+	-	+	-	-	+	-	+	+	-	-	-	+	-	-	-	+	-	-	-	-	-	+	-	+	-	-
121443	not determinedn.d.	-	-	-	+	+	+	-	-	+/-	+	+	+	-	-	-	-	-	-	-	-	+/-	+/-	+	-	+/-	+/-	-	+	+/-	+	+	-	-	-	+	-	-	-	+	+/-	-	-	-	-	+/-	-	+/-	-	-

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Host	#Human	-
#Host Body Product	#Oral cavity and Airways	#Saliva

Isolation

@ref	Sample type	Host species
1854	human saliva	Homo sapiens
53648	Human saliva	Homo sapiens
67770	Human saliva	Homo sapiens
121443	Human, Saliva	Homo sapiens

Taxonmaps

69479

Global distribution of 16S sequence X94229 (>99% sequence identity) for Limosilactobacillus oris from Microbeatlas

Aquatic Samples	630
Soil Samples	440
Animal Samples	32291
Plant Samples	233
Total Samples	33594

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
1854	1	Risk group (German classification) According to TRBA 466
121443	1	Risk group (French classification) According to TRBA 466

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
67770	ASM143446v1 assembly for Limosilactobacillus oris DSM 4864	contig	GCA_001434465	1423779.3	2663763371	1423779	64.52

16S sequences

@ref	Description	Accession	Length	NCBI tax ID
20218	Lactobacillus oris strain DSM 4864 16S-23S ribosomal RNA intergenic spacer and 23S ribosomal RNA gene, partial sequence	EU161599	792	1632
20218	L.oris 16S rRNA gene	X61131	1359	1632
1854	L.oris 16S ribosomal RNA	X94229	1512	1632
67770	Lactobacillus oris gene for 16S ribosomal RNA, partial sequence, strain: JCM 11028	LC097079	1511	1632
124043	Lactobacillus oris gene for 16S rRNA, partial sequence, strain: JCM 11028.	AB289207	645	1632

GC content

@ref	GC-content (mol%)	Method
1854	49.3
67770	50	genome sequence analysis
67770	49.3	thermal denaturation, midpoint method (Tm)

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: ASM143446v1 assembly for Limosilactobacillus oris DSM 4864
NCBI: GCA_001434465

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	oxygen_tolerance	BacteriaNetⓘ	anaerobe	71.96	no
125439	gram_stain	BacteriaNetⓘ	positive	49.11	no
125439	motility	BacteriaNetⓘ	no	68.81	no
125439	spore_formation	BacteriaNetⓘ	no	83.42	no

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Lactobacillus oris DSM 4864
PATRIC: 1423779.3

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	yes	95.41	no
125438	anaerobic	anaerobicⓘ	no	69.30	no
125438	spore-forming	spore-formingⓘ	no	92.18	no
125438	aerobic	aerobicⓘ	no	93.32	no
125438	thermophilic	thermophileⓘ	no	97.00	yes
125438	flagellated	motile2+ⓘ	no	93.50	no

Literature

Title	Authors	Journal	DOI	Year
Susceptibility of Lactobacillaceae Strains to Aminoglycoside Antibiotics in the Light of EFSA Guidelines.	Dec M, Herman-Ostrzyzek K, Zomer A, Urban-Chmiel R.	Life (Basel)	10.3390/life15050732	2025

References

#1854	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 4864
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#20218	Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )
#34712	; Curators of the CIP;
#53648	Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 37396
#67770	Japan Collection of Microorganism (JCM) ; Curators of the JCM;
#68371	Automatically annotated from API 50CH acid .
#68382	Automatically annotated from API zym .
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#121443	Collection of Institut Pasteur ; Curators of the CIP; CIP 105162
#124043	Isabel Schober, Julia Koblitz: Data extracted from sequence databases, automatically matched based on designation and taxonomy .
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

Content

About us

Limosilactobacillus oris (DSM 4864, ATCC 49062, CIP 105162)

Name and taxonomic classification

Morphology

Cell morphology

Culture and growth conditions

Culture medium

Culture temp

Physiology and metabolism

Oxygen tolerance

Spore formation

Murein

Metabolite utilization

Metabolite tests

Enzymes

API zym

API 50CHac

Isolation, sampling and environmental information

Isolation source categories

Isolation

Taxonmaps

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_001434465

16S sequences

GC content

Genome-based predictions

Predictions

Literature

Literature

References

BacDive

Help

Social Media