Name: Lacticaseibacillus paracasei subsp. tolerans 7/74
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 6434

Type strain:

Species: Lacticaseibacillus paracasei subsp. tolerans

Strain Designation: 7/74

Culture col. no.: DSM 20258, ATCC 25599, NCIB 9709, CCUG 34829, JCM 1171, BCRC 14628, CCM 7092, CECT 4175, CIP 102994, CIP 103024, IFO 15906, KCTC 3074, LMG 9191, NBRC 15906, NCFB 2742, NCIMB 9709, NRIC 1045, NCDO 2742, NCFB 2774

Strain history: CIP <- 1987, NCIB <- O. Kandler: strain 7-74

NCBI tax ID(s): 1423830 (strain), 113557 (subspecies)

SI-ID 3342

LMG 9191, ATCC 25599, JCM 1171, NCFB 2742, NCIMB 9709, NCDO 2774, NCFB 2774, NCIB 9709, CCUG 34829, NCDO 2742, CIP 103024, CCRC 14628, KCTC 3074, IFO15906, CECT 4175, NBRC 15906, CCM 7092, CIP 102994, BCRC 14628, KCTC 5058, DSM 20258

Other strains from Lacticaseibacillus paracasei subsp. tolerans

L. paracasei subsp. tolerans 19/74, 19-74, DSM 20012, NCDO 1974, ...

Section

Lacticaseibacillus paracasei subsp. tolerans 7/74 is a facultative anaerobe, mesophilic, Gram-positive bacterium that was isolated from pasteurized milk.

Gram-positive
rod-shaped
facultative anaerobe
mesophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Bacillota*
	Class	*Bacilli*
	Order	*Lactobacillales*
	Family	*Lactobacillaceae*
	Genus	*Lacticaseibacillus*
	Species	**Lacticaseibacillus paracasei subsp. tolerans**
	Full Scientific Name (LPSN)	Lacticaseibacillus paracasei subsp. tolerans (Abo-Elnaga and Kandler 1965) Zheng et al. 2020

	Synonym	Lactobacillus casei subsp. tolerans
	Synonym	Lactobacillus paracasei subsp. tolerans

Information on morphological and physiological properties Morphology

[Ref.: #121545]	Gram stain	positive

[Ref.: #121545]	Cell shape	rod-shaped

[Ref.: #121545]	Motility	no

Information on culture and growth conditions Culture and growth conditions

[Ref.: #8667]

Culture medium

MRS MEDIUM (DSMZ Medium 11)

[Ref.: #8667]

Culture medium growth

[Ref.: #8667]

Culture medium link

Medium recipe at MediaDive

[Ref.: #8667]

Culture medium composition

expand / minimize

[Ref.: #38115]

Culture medium

MEDIUM 40- for Lactobacillus and Leuconostoc

[Ref.: #38115]

Culture medium growth

[Ref.: #38115]

Culture medium composition

expand / minimize

[Ref.: #121545]

Culture medium

CIP Medium 40

[Ref.: #121545]

Culture medium growth

[Ref.: #121545]

Culture medium link

Medium recipe provided by DSMZ

	Kind of temperature		Temperature
[Ref.: #8667]		growth	30 °C
[Ref.: #38115]		growth	30 °C
[Ref.: #52355]		growth	30 °C
[Ref.: #67770]		growth	30 °C
[Ref.: #121545]		growth	10-37 °C
[Ref.: #121545]	no	growth	45 °C

[Ref.: #8667]	Temperature range	mesophilic
[Ref.: #38115]	Temperature range	mesophilic
[Ref.: #52355]	Temperature range	mesophilic
[Ref.: #67770]	Temperature range	mesophilic
[Ref.: #121545]	Temperature range	thermophilic

Information on physiology and metabolism Physiology and metabolism

[Ref.: #121545]

Oxygen tolerance

facultative anaerobe

[Ref.: #8667]	Murein short key	A11.31
[Ref.: #8667]	Murein types	A4alpha L-Lys-D-Asp

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #68371]	amygdalin ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	arbutin ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	cellobiose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-arabinose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-arabitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-fucose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-lyxose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-mannitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-ribose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-sorbitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-tagatose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-xylose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	erythritol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	esculin ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	galactitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	gentiobiose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	gluconate ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	glycerol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	glycogen ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	inulin ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-arabinose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-arabitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-fucose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-rhamnose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-sorbose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-xylose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	maltose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	melezitose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	melibiose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	methyl alpha-D-glucopyranoside ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	methyl alpha-D-mannoside ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	methyl beta-D-xylopyranoside ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	myo-inositol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #121545]	nitrate ChEBI	-	reduction
[Ref.: #121545]	nitrate ChEBI	+	respiration
[Ref.: #121545]	nitrite ChEBI	-	reduction
[Ref.: #68371]	Potassium 2-ketogluconate ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	Potassium 5-ketogluconate ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	raffinose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	ribitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	salicin ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	starch ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	sucrose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	trehalose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	turanose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	xylitol ChEBI	-	builds acid from	* from API 50CH acid
	Only first 10 entries are displayed. Click here to see all.

	Physiological tests	Metabolite	Voges-Proskauer-test
[Ref.: #121545]		acetoin ChEBI	-

	Enzyme	Enzyme activity	EC number
[Ref.: #68382]	acid phosphatase	+	3.1.3.2	* from API zym
[Ref.: #121545]	alcohol dehydrogenase	-	1.1.1.1
[Ref.: #68382]	alkaline phosphatase	-	3.1.3.1	* from API zym
[Ref.: #68382]	alpha-chymotrypsin	-	3.4.21.1	* from API zym
[Ref.: #68382]	alpha-fucosidase	-	3.2.1.51	* from API zym
[Ref.: #68382]	alpha-galactosidase	-	3.2.1.22	* from API zym
[Ref.: #68382]	alpha-glucosidase	-	3.2.1.20	* from API zym
[Ref.: #68382]	alpha-mannosidase	-	3.2.1.24	* from API zym
[Ref.: #68382]	beta-galactosidase	+	3.2.1.23	* from API zym
[Ref.: #68382]	beta-glucosidase	-	3.2.1.21	* from API zym
[Ref.: #68382]	beta-glucuronidase	-	3.2.1.31	* from API zym
[Ref.: #121545]	catalase	-	1.11.1.6
[Ref.: #68382]	cystine arylamidase	-	3.4.11.3	* from API zym
[Ref.: #68382]	esterase (C 4)	-		* from API zym
[Ref.: #68382]	esterase lipase (C 8)	-		* from API zym
[Ref.: #68382]	leucine arylamidase	+	3.4.11.1	* from API zym
[Ref.: #68382]	lipase (C 14)	-		* from API zym
[Ref.: #121545]	lysine decarboxylase	-	4.1.1.18
[Ref.: #68382]	N-acetyl-beta-glucosaminidase	-	3.2.1.52	* from API zym
[Ref.: #68382]	naphthol-AS-BI-phosphohydrolase	+		* from API zym
[Ref.: #121545]	ornithine decarboxylase	-	4.1.1.17
[Ref.: #121545]	oxidase	-
[Ref.: #68382]	trypsin	-	3.4.21.4	* from API zym
[Ref.: #68382]	valine arylamidase	+		* from API zym
	Only first 10 entries are displayed. Click here to see all.

API® 50CH acid:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	ControlQ	Acid from glycerolGLY	Acid from erythritolERY	Acid from D-arabinoseDARA	Acid from L-arabinoseLARA	Acid from D-riboseRIB	Acid from D-xyloseDXYL	Acid from L-xyloseLXYL	Acid from D-adonitolADO	Acid from methyl beta-D-xylopyranosideMDX	Acid from D-galactoseGAL	Acid from D-glucoseGLU	Acid from D-fructoseFRU	Acid from D-mannoseMNE	Acid from L-sorboseSBE	Acid from L-rhamnoseRHA	Acid from dulcitolDUL	Acid from inositolINO	Acid from D-mannitolMAN	Acid from D-sorbitolSOR	Acid from methyl alpha-D-mannopyranosideMDM	Acid from methyl alpha-D-glucopyranosideMDG	Acid from N-acetyl-glucosamineNAG	Acid from amygdalinAMY	Acid from arbutinARB	Acid from esculinESC	Acid from salicinSAL	Acid from cellobioseCEL	Acid from maltoseMAL	Acid from lactoseLAC	Acid from melibioseMEL	Acid from sucroseSAC	Acid from trehaloseTRE	Acid from inulinINU	Acid from melezitoseMLZ	Acid from raffinoseRAF	Acid from starchAMD	Acid from glycogenGLYG	Acid from xylitolXLT	Acid from gentiobioseGEN	Acid from turanoseTUR	Acid from D-lyxoseLYX	Acid from D-tagatoseTAG	Acid from D-fucoseDFUC	Acid from L-fucoseLFUC	Acid from D-arabitolDARL	Acid from L-arabitolLARL	Acid from gluconateGNT	Acid from 2-Ketogluconate2KG	Acid from 5-Ketogluconate5KG
[Ref.: #8667]	14606	-	-	-	-	-	-	-	-	-	-	+	+	+	+	-	-	-	-	-	-	-	-	+	-	-	-	-	-	-	+	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-
[Ref.: #121545]	9138	not determinedn.d.	-	-	-	-	-	-	-	-	-	+/-	+/-	-	+/-	-	-	-	-	-	-	-	-	+/-	-	-	-	-	-	-	+/-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #121545]	7317	-	-	-	-	-	+	+	-	-	-	+	+	-	+	-	-	-	-	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #8667]	Sample type/isolated from	pasteurized milk

[Ref.: #52355]	Sample type/isolated from	Milk,pasteurized

[Ref.: #67770]	Sample type/isolated from	Pasteurized milk

[Ref.: #121545]	Sample type/isolated from	Food, Pasteurized milk
* marker position based on {}

Isolation sources categories

#Engineered	#Food production	#Dairy product
#Engineered	#Treatment	#Preserved
#Engineered	#Treatment	#Sterilized (Desinfected)

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence LC065035 (>99% sequence identity) for Lactobacillaceae from Microbeatlas

Aquatic Samples	2397
Soil Samples	1801
Animal Samples	39363
Plant Samples	883
Total Samples	44444

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #8667]	Biosafety level	1	Risk group (German classification) According to TRBA 466
[Ref.: #121545]	Biosafety level	1	Risk group (French classification)

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

Only first 5 entries are displayed. Click here to see all.

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #67770]	Lactobacillus paracasei subsp. tolerans gene for 16S ribosomal RNA, partial sequence, strain: JCM 1171	LC065035	1467	ENA	113557 tax ID
[Ref.: #20218]	Lactobacillus paracasei gene, 16S-23S rRNA intergenic spacer region and 23S rRNA gene 5' flanking region, partial sequence	AB035487	670	ENA	1597 tax ID	*
[Ref.: #20218]	Lactobacillus paracasei subsp. tolerans genes for 16S rRNA, 16S-23S internal transcribed spacer, tRNA-Ile, tRNA-Ala, 23S rRNA, partial and complete sequence, strain:JCM 1171	AB237502	572	ENA	113557 tax ID	*
[Ref.: #20218]	Lactobacillus paracasei subsp. tolerans genes for 16S rRNA, 16S-23S internal transcribed spacer, 23S rRNA, partial sequence, strain:JCM 1171	AB237503	364	ENA	113557 tax ID	*
[Ref.: #20218]	Lactobacillus paracasei subsp. tolerans gene for 16S rRNA, partial sequence, strain: JCM 1171	AB289229	623	ENA	113557 tax ID	*
[Ref.: #20218]	Lactobacillus casei gene for 16S rRNA	D16550	1520	ENA	1582 tax ID	*
[Ref.: #20218]	Lactobacillus paracasei subsp. tolerans gene for 16S rRNA, partial sequence	AB181950	1497	ENA	113557 tax ID	*
[Ref.: #20218]	Lactobacillus paracasei subsp. tolerans gene for 16S rRNA, partial sequence, strain: NBRC 15906	AB680995	1497	ENA	113557 tax ID	*

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #67770]	Lacticaseibacillus paracasei subsp. tolerans DSM 20258	GCA_001436485	scaffold	GenBank	1423830 tax ID
[Ref.: #66792]	Lacticaseibacillus paracasei subsp. tolerans NBRC 15906	GCA_007989185	contig	GenBank	113557 tax ID	*
[Ref.: #66792]	Lactobacillus paracasei subsp. tolerans DSM 20258	1423830.3	wgs	PATRIC	1423830 tax ID	*
[Ref.: #66792]	Lactobacillus paracasei subsp. tolerans strain NBRC 15906	113557.4	wgs	PATRIC	113557 tax ID	*
[Ref.: #66792]	Lacticaseibacillus paracasei tolerans DSM 20258	2657245104	draft	JGI IMG/M	1423830 tax ID	*

[Ref.: #8667]	GC-content	46.9 mol%
[Ref.: #67770]	GC-content	46.4 mol%	genome sequence analysis

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Lactobacillus paracasei subsp. tolerans DSM 20258 PATRIC: 1423830.3
[Ref.: #69480]	motile	no	93.97	no
[Ref.: #69480]	gram-positive	yes	96.817	no
[Ref.: #69480]	anaerobic	no	93.681	no
[Ref.: #69480]	halophile	yes	83.756	no
[Ref.: #69480]	spore-forming	no	92.784	no
[Ref.: #69480]	glucose-util	yes	89.679	no
[Ref.: #69480]	thermophile	no	98.661	yes
[Ref.: #69480]	aerobic	no	96.26	no
[Ref.: #69480]	flagellated	no	97.549	no
[Ref.: #69480]	glucose-ferment	yes	91.022	no

Availability in culture collections External links

[Ref.: #8667]

Culture collection no.

DSM 20258, ATCC 25599, NCIB 9709, CCUG 34829, JCM 1171, BCRC 14628, CCM 7092, CECT 4175, CIP 102994, CIP 103024, IFO 15906, KCTC 3074, LMG 9191, NBRC 15906, NCFB 2742, NCIMB 9709, NRIC 1045, NCDO 2742, NCFB 2774

[Ref.: #75908]

SI-ID 3342

References

#8667

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 20258

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#20218

Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )

#38115 ; Curators of the CIP;
#52355 Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 34829

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#68371

Automatically annotated from API 50CH acid .

#68382

Automatically annotated from API zym .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#75908

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID3342.1 )

#121545 Collection of Institut Pasteur ; Curators of the CIP; CIP 103024
* These data were automatically processed and therefore are not curated

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Lactobacillus paracasei subsp. tolerans DSM 20258

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Change proposal