Lacticaseibacillus paracasei subsp. tolerans (DSM 20258, ATCC 25599, NCIB 9709)

Name: Lacticaseibacillus paracasei subsp. tolerans (DSM 20258, ATCC 25599, NCIB 9709)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 6434

Type strain

Strain Designation 7/74

Culture col. no. DSM 20258 ATCC 25599 NCIB 9709 CCUG 34829 7/74 15 more

NCBI tax ID(s) 1423830 113557

Special Collections DSMZ CCUG JCM KCTC CIP

History <- ATCC <- I.G. Abo-Elnaga, 7/74 T. Mitsuoka S8-12 <-- DSM 20258 <-- ATCC 25599 <-- I. G. Abo-Elnaga 7/74. CIP <- 1987, NCIB <- O. Kandler: strain 7-74

Links

Lacticaseibacillus paracasei subsp. tolerans 7/74 is a facultative anaerobe, Gram-positive, rod-shaped bacterium that was isolated from pasteurized milk.

Gram-positive rod-shaped facultative anaerobe genome sequence 16S sequence Bacteria

Name and taxonomic classification

SI-ID 3342

LMG 9191,ATCC 25599,JCM 1171,NCFB 2742,NCIMB 9709,NCDO 2774,NCFB 2774,NCIB 9709,CCUG 34829,NCDO 2742,CIP 103024,CCRC 14628,KCTC 3074,IFO15906,CECT 4175,NBRC 15906,CCM 7092,CIP 102994,BCRC 14628,KCTC 5058,DSM 20258

@ref 20215

Last LPSN update 2026-02-09 11:19:13

Domain Bacteria

Phylum Bacillota

Class Bacilli

Order Lactobacillales

Family Lactobacillaceae

Genus Lacticaseibacillus

Species Lacticaseibacillus paracasei subsp. tolerans

Full scientific name Lacticaseibacillus paracasei subsp. tolerans (Abo-Elnaga and Kandler 1965) Zheng et al. 2020

Synonyms (2)

Lactobacillus casei subsp. tolerans
Lactobacillus paracasei subsp. tolerans

BacDive ID	Other strains from **Lacticaseibacillus paracasei subsp. tolerans** (1)	Type strain
6433	L. paracasei subsp. tolerans 19/74, 19-74, DSM 20012, NCDO 1974, JCM 1172, ...

Morphology

Cell morphology

@ref	Gram stain	Cell shape	Confidence
121545	positive	rod-shaped
125438			90.5
125438	positive		92.093

Culture and growth conditions

Culture medium

@ref	Name	Medium link	Composition
8667	MRS MEDIUM (DSMZ Medium 11)	Medium recipe at MediaDive	Name: MRS MEDIUM (DSMZ Medium 11) Composition: Glucose 20.0 g/l Casein peptone 10.0 g/l Meat extract 10.0 g/l Na-acetate 5.0 g/l Yeast extract 5.0 g/l (NH4)3 citrate 2.0 g/l K2HPO4 2.0 g/l Tween 80 1.0 g/l MgSO4 x 7 H2O 0.2 g/l MnSO4 x H2O 0.05 g/l Distilled water
38115	MEDIUM 40- for Lactobacillus and Leuconostoc		Distilled water make up to (1000.000 ml);Man Rogosa Sharp agar (68.000 g)
121545	CIP Medium 40	Medium recipe at CIP

Culture temp

@ref	Growth	Type	Temperature (°C)
8667	positive	growth	30
38115	positive	growth	30
52355	positive	growth	30
67770	positive	growth	30
121545	positive	growth	10-37
121545	negative	growth	45

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance	Confidence
121545	facultative anaerobe
125439	obligate aerobe	98.2

Murein

@ref	Murein short key	Type
8667	A11.31	A4alpha L-Lys-D-Asp

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
68371	27613 ChEBI	amygdalin	-	builds acid from	from API 50CH acid
68371	18305 ChEBI	arbutin	-	builds acid from	from API 50CH acid
68371	17057 ChEBI	cellobiose	-	builds acid from	from API 50CH acid
68371	17108 ChEBI	D-arabinose	-	builds acid from	from API 50CH acid
68371	18333 ChEBI	D-arabitol	-	builds acid from	from API 50CH acid
68371	28847 ChEBI	D-fucose	-	builds acid from	from API 50CH acid
68371	62318 ChEBI	D-lyxose	-	builds acid from	from API 50CH acid
68371	16899 ChEBI	D-mannitol	-	builds acid from	from API 50CH acid
68371	16988 ChEBI	D-ribose	-	builds acid from	from API 50CH acid
68371	17924 ChEBI	D-sorbitol	-	builds acid from	from API 50CH acid
68371	16443 ChEBI	D-tagatose	-	builds acid from	from API 50CH acid
68371	65327 ChEBI	D-xylose	-	builds acid from	from API 50CH acid
68371	17113 ChEBI	erythritol	-	builds acid from	from API 50CH acid
68371	4853 ChEBI	esculin	-	builds acid from	from API 50CH acid
68371	16813 ChEBI	galactitol	-	builds acid from	from API 50CH acid
68371	28066 ChEBI	gentiobiose	-	builds acid from	from API 50CH acid
68371	24265 ChEBI	gluconate	-	builds acid from	from API 50CH acid
68371	17754 ChEBI	glycerol	-	builds acid from	from API 50CH acid
68371	28087 ChEBI	glycogen	-	builds acid from	from API 50CH acid
68371	15443 ChEBI	inulin	-	builds acid from	from API 50CH acid
68371	30849 ChEBI	L-arabinose	-	builds acid from	from API 50CH acid
68371	18403 ChEBI	L-arabitol	-	builds acid from	from API 50CH acid
68371	18287 ChEBI	L-fucose	-	builds acid from	from API 50CH acid
68371	62345 ChEBI	L-rhamnose	-	builds acid from	from API 50CH acid
68371	17266 ChEBI	L-sorbose	-	builds acid from	from API 50CH acid
68371	65328 ChEBI	L-xylose	-	builds acid from	from API 50CH acid
68371	17306 ChEBI	maltose	-	builds acid from	from API 50CH acid
68371	6731 ChEBI	melezitose	-	builds acid from	from API 50CH acid
68371	28053 ChEBI	melibiose	-	builds acid from	from API 50CH acid
68371	320061 ChEBI	methyl alpha-D-glucopyranoside	-	builds acid from	from API 50CH acid
68371	43943 ChEBI	methyl alpha-D-mannoside	-	builds acid from	from API 50CH acid
68371	74863 ChEBI	methyl beta-D-xylopyranoside	-	builds acid from	from API 50CH acid
68371	17268 ChEBI	myo-inositol	-	builds acid from	from API 50CH acid
121545	17632 ChEBI	nitrate	-	reduction
121545	17632 ChEBI	nitrate	+	respiration
121545	16301 ChEBI	nitrite	-	reduction
68371		Potassium 2-ketogluconate	-	builds acid from	from API 50CH acid
68371		Potassium 5-ketogluconate	-	builds acid from	from API 50CH acid
68371	16634 ChEBI	raffinose	-	builds acid from	from API 50CH acid
68371	15963 ChEBI	ribitol	-	builds acid from	from API 50CH acid
68371	17814 ChEBI	salicin	-	builds acid from	from API 50CH acid
68371	28017 ChEBI	starch	-	builds acid from	from API 50CH acid
68371	17992 ChEBI	sucrose	-	builds acid from	from API 50CH acid
68371	27082 ChEBI	trehalose	-	builds acid from	from API 50CH acid
68371	32528 ChEBI	turanose	-	builds acid from	from API 50CH acid
68371	17151 ChEBI	xylitol	-	builds acid from	from API 50CH acid

Metabolite tests

@ref	Chebi-ID	Metabolite	Voges-proskauer-test
121545	15688 ChEBI	acetoin	-

Enzymes

@ref	Value	Activity	Ec
68382	acid phosphatase	+	3.1.3.2	from API zym
121545	alcohol dehydrogenase	-	1.1.1.1
68382	alkaline phosphatase	-	3.1.3.1	from API zym
68382	alpha-chymotrypsin	-	3.4.21.1	from API zym
68382	alpha-fucosidase	-	3.2.1.51	from API zym
68382	alpha-galactosidase	-	3.2.1.22	from API zym
68382	alpha-glucosidase	-	3.2.1.20	from API zym
68382	alpha-mannosidase	-	3.2.1.24	from API zym
68382	beta-galactosidase	+	3.2.1.23	from API zym
68382	beta-glucosidase	-	3.2.1.21	from API zym
68382	beta-glucuronidase	-	3.2.1.31	from API zym
121545	catalase	-	1.11.1.6
68382	cystine arylamidase	-	3.4.11.3	from API zym
68382	esterase (C 4)	-		from API zym
68382	esterase lipase (C 8)	-		from API zym
68382	leucine arylamidase	+	3.4.11.1	from API zym
68382	lipase (C 14)	-		from API zym
121545	lysine decarboxylase	-	4.1.1.18
68382	N-acetyl-beta-glucosaminidase	-	3.2.1.52	from API zym
68382	naphthol-AS-BI-phosphohydrolase	+		from API zym
121545	ornithine decarboxylase	-	4.1.1.17
121545	oxidase	-
68382	trypsin	-	3.4.21.4	from API zym
68382	valine arylamidase	+		from API zym

API zym

@ref	Control	Alkaline phosphatase	Esterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	Lipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	Trypsin	alpha- Chymotrypsin	Acid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	alpha- Galactosidase	beta- Galactosidase	beta- Glucuronidase	alpha- Glucosidase	beta- Glucosidase	N-acetyl-beta- glucosaminidase	alpha- Mannosidase	alpha- Fucosidase
121545	-	-	-	-	-	+	+	-	-	-	+	+	-	+	-	-	-	-	-	-

API 50CHac

@ref	ControlQ	GLY	ERY	DARA	LARA	RIB	DXYL	LXYL	ADO	MDX	GAL	GLU	FRU	MNE	SBE	RHA	DUL	INO	MAN	SOR	MDM	MDG	NAG	AMY	ARB	ESC	SAL	CEL	MAL	LAC	MEL	SAC	TRE	INU	MLZ	RAF	AMD	GLYG	XLT	GEN	TUR	LYX	TAG	DFUC	LFUC	DARL	LARL	GNT	2KG	5KG
8667	-	-	-	-	-	-	-	-	-	-	+	+	+	+	-	-	-	-	-	-	-	-	+	-	-	-	-	-	-	+	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-
121545	not determinedn.d.	-	-	-	-	-	-	-	-	-	+/-	+/-	-	+/-	-	-	-	-	-	-	-	-	+/-	-	-	-	-	-	-	+/-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Engineered	#Food production	#Dairy product
#Engineered	#Treatment	#Preserved
#Engineered	#Treatment	#Sterilized (Desinfected)

Isolation

@ref	Sample type
8667	pasteurized milk
52355	Milk,pasteurized
67770	Pasteurized milk
121545	Food, Pasteurized milk

Taxonmaps

69479

Global distribution of 16S sequence LC065035 (>99% sequence identity) for Lactobacillaceae from Microbeatlas

Aquatic Samples	2397
Soil Samples	1801
Animal Samples	39363
Plant Samples	883
Total Samples	44444

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
8667	1	Risk group (German classification) According to TRBA 466
121545	1	Risk group (French classification) According to TRBA 466

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID
66792	ASM798918v1 assembly for Lacticaseibacillus paracasei subsp. tolerans NBRC 15906	contig	GCA_007989185	113557.4		113557
67770	ASM143648v1 assembly for Lacticaseibacillus paracasei subsp. tolerans DSM 20258	scaffold	GCA_001436485	1423830.3	2657245104	1423830

16S sequences

@ref	Description	Accession	Length	NCBI tax ID
20218	Lactobacillus paracasei gene, 16S-23S rRNA intergenic spacer region and 23S rRNA gene 5' flanking region, partial sequence	AB035487	670	1597
20218	Lactobacillus paracasei subsp. tolerans genes for 16S rRNA, 16S-23S internal transcribed spacer, tRNA-Ile, tRNA-Ala, 23S rRNA, partial and complete sequence, strain:JCM 1171	AB237502	572	113557
20218	Lactobacillus paracasei subsp. tolerans genes for 16S rRNA, 16S-23S internal transcribed spacer, 23S rRNA, partial sequence, strain:JCM 1171	AB237503	364	113557
20218	Lactobacillus paracasei subsp. tolerans gene for 16S rRNA, partial sequence, strain: JCM 1171	AB289229	623	113557
20218	Lactobacillus casei gene for 16S rRNA	D16550	1520	1582
20218	Lactobacillus paracasei subsp. tolerans gene for 16S rRNA, partial sequence	AB181950	1497	113557
20218	Lactobacillus paracasei subsp. tolerans gene for 16S rRNA, partial sequence, strain: NBRC 15906	AB680995	1497	113557
67770	Lactobacillus paracasei subsp. tolerans gene for 16S ribosomal RNA, partial sequence, strain: JCM 1171	LC065035	1467	113557

GC content

@ref	GC-content (mol%)	Method
8667	46.9
67770	46.4	genome sequence analysis

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: Lacticaseibacillus paracasei subsp. tolerans DSM 20258
NCBI: GCA_001436485

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	oxygen_tolerance	BacteriaNetⓘ	obligate aerobe	98.20	no
125439	gram_stain	BacteriaNetⓘ	positive	64.00	no
125439	motility	BacteriaNetⓘ	no	59.70	no
125439	spore_formation	BacteriaNetⓘ	no	85.40	no

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Lactobacillus paracasei subsp. tolerans DSM 20258
PATRIC: 1423830.3

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	yes	92.09	no
125438	anaerobic	anaerobicⓘ	no	86.07	no
125438	spore-forming	spore-formingⓘ	no	81.27	no
125438	aerobic	aerobicⓘ	no	94.88	yes
125438	thermophilic	thermophileⓘ	no	96.50	yes
125438	flagellated	motile2+ⓘ	no	90.50	no

Literature

Topic	Title	Authors	Journal	DOI	Year
	Probiotic Properties of Lactic Acid Bacteria Newly Isolated from Algerian Raw Cow's Milk.	Kouadri Boudjelthia N, Belabbas M, Bekenniche N, Monnoye M, Gerard P, Riazi A.	Microorganisms	10.3390/microorganisms11082091	2023
	Clinical Potential of Novel Microbial Therapeutic LP51 Based on Xerosis-Microbiome Index.	Kim S, Rahim MA, Tajdozian H, Barman I, Park HA, Yoon Y, Jo S, Lee S, Shuvo MSH, Bae SH, Lee H, Ju S, Park CE, Kim HK, Han JH, Kim JW, Yoon SG, Kim JH, Choi YG, Lee S, Seo H, Song HY.	Cells	10.3390/cells13232029	2024
Genetics	Establishment of a polymerase chain reaction-based method for strain-level management of Enterococcus faecalis EF-2001 using species-specific sequences identified by whole genome sequences.	Hamamoto H, Ogasawara AA, Iwasa M, Sekimizu K.	Front Microbiol	10.3389/fmicb.2022.959063	2022
	Lactobacillus paracasei-derived extracellular vesicles attenuate the intestinal inflammatory response by augmenting the endoplasmic reticulum stress pathway.	Choi JH, Moon CM, Shin TS, Kim EK, McDowell A, Jo MK, Joo YH, Kim SE, Jung HK, Shim KN, Jung SA, Kim YK.	Exp Mol Med	10.1038/s12276-019-0359-3	2020
	Occurrence and genetic diversity of prophage sequences identified in the genomes of L. casei group bacteria.	Jarocki P, Komon-Janczara E, Mlodzinska A, Sadurski J, Kolodzinska K, Laczmanski L, Panek J, Frac M.	Sci Rep	10.1038/s41598-023-35823-z	2023
	Potential Probiotic Properties of Exopolysaccharide-Producing Lacticaseibacillus paracasei EPS DA-BACS and Prebiotic Activity of Its Exopolysaccharide.	Lee MG, Joeng H, Shin J, Kim S, Lee C, Song Y, Lee BH, Park HG, Lee TH, Jiang HH, Han YS, Lee BG, Lee HJ, Park MJ, Jun YJ, Park YS.	Microorganisms	10.3390/microorganisms10122431	2022
	Comparison of Real-Time PCR and Droplet Digital PCR for the Quantitative Detection of Lactiplantibacillus plantarum subsp. plantarum	Choi CH, Kim E, Yang SM, Kim DS, Suh SM, Lee GY, Kim HY.	Foods	10.3390/foods11091331	2022
	Potential Probiotic Lacticaseibacillus paracasei MJM60396 Prevents Hyperuricemia in a Multiple Way by Absorbing Purine, Suppressing Xanthine Oxidase and Regulating Urate Excretion in Mice.	Lee Y, Werlinger P, Suh JW, Cheng J.	Microorganisms	10.3390/microorganisms10050851	2022

References

#8667	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 20258
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#20218	Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )
#38115	; Curators of the CIP;
#52355	Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 34829
#66792	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .
#67770	Japan Collection of Microorganism (JCM) ; Curators of the JCM;
#68371	Automatically annotated from API 50CH acid .
#68382	Automatically annotated from API zym .
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#121545	Collection of Institut Pasteur ; Curators of the CIP; CIP 103024
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Lacticaseibacillus paracasei subsp. tolerans (DSM 20258, ATCC 25599, NCIB 9709)

Name and taxonomic classification

Morphology

Cell morphology

Culture and growth conditions

Culture medium

Culture temp

Physiology and metabolism

Oxygen tolerance

Murein

Metabolite utilization

Metabolite tests

Enzymes

API zym

API 50CHac

Isolation, sampling and environmental information

Isolation source categories

Isolation

Taxonmaps

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_007989185

16S sequences

GC content

Genome-based predictions

Predictions

Literature

Literature

References

BacDive

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