Strain identifier

BacDive ID: 6434

Type strain: Yes

Species: Lacticaseibacillus paracasei subsp. tolerans

Strain Designation: 7/74

Strain history: CIP <- 1987, NCIB <- O. Kandler: strain 7-74

NCBI tax ID(s): 1423830 (strain), 113557 (subspecies)

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General

@ref: 8667

BacDive-ID: 6434

DSM-Number: 20258

keywords: genome sequence, 16S sequence, Bacteria, facultative anaerobe, mesophilic, Gram-positive, rod-shaped

description: Lacticaseibacillus paracasei subsp. tolerans 7/74 is a facultative anaerobe, mesophilic, Gram-positive bacterium that was isolated from pasteurized milk.

NCBI tax id

NCBI tax idMatching level
1423830strain
113557subspecies

strain history

@refhistory
8667<- ATCC <- I.G. Abo-Elnaga, 7/74
67770T. Mitsuoka S8-12 <-- DSM 20258 <-- ATCC 25599 <-- I. G. Abo-Elnaga 7/74.
121545CIP <- 1987, NCIB <- O. Kandler: strain 7-74

doi: 10.13145/bacdive6434.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Lactobacillales
  • family: Lactobacillaceae
  • genus: Lacticaseibacillus
  • species: Lacticaseibacillus paracasei subsp. tolerans
  • full scientific name: Lacticaseibacillus paracasei subsp. tolerans (Abo-Elnaga and Kandler 1965) Zheng et al. 2020

  • synonyms

    @refsynonym
    20215Lactobacillus casei subsp. tolerans
    20215Lactobacillus paracasei subsp. tolerans

@ref: 8667

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Lactobacillales

family: Lactobacillaceae

genus: Lacticaseibacillus

species: Lacticaseibacillus paracasei subsp. tolerans

full scientific name: Lacticaseibacillus paracasei subsp. tolerans (Abo-Elnaga and Kandler 1965) Zheng et al. 2020

strain designation: 7/74

type strain: yes

Morphology

cell morphology

  • @ref: 121545
  • gram stain: positive
  • cell shape: rod-shaped
  • motility: no

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
8667MRS MEDIUM (DSMZ Medium 11)yeshttps://mediadive.dsmz.de/medium/11Name: MRS MEDIUM (DSMZ Medium 11) Composition: Glucose 20.0 g/l Casein peptone 10.0 g/l Meat extract 10.0 g/l Na-acetate 5.0 g/l Yeast extract 5.0 g/l (NH4)3 citrate 2.0 g/l K2HPO4 2.0 g/l Tween 80 1.0 g/l MgSO4 x 7 H2O 0.2 g/l MnSO4 x H2O 0.05 g/l Distilled water
38115MEDIUM 40- for Lactobacillus and LeuconostocyesDistilled water make up to (1000.000 ml);Man Rogosa Sharp agar (68.000 g)
121545CIP Medium 40yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=40

culture temp

@refgrowthtypetemperaturerange
8667positivegrowth30mesophilic
38115positivegrowth30mesophilic
52355positivegrowth30mesophilic
67770positivegrowth30mesophilic
121545positivegrowth10-37
121545nogrowth45thermophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 121545
  • oxygen tolerance: facultative anaerobe

murein

  • @ref: 8667
  • murein short key: A11.31
  • type: A4alpha L-Lys-D-Asp

metabolite utilization

@refmetaboliteutilization activitykind of utilization testedChebi-ID
68371Potassium 5-ketogluconate-builds acid from
68371Potassium 2-ketogluconate-builds acid from
68371gluconate-builds acid from24265
68371L-arabitol-builds acid from18403
68371D-arabitol-builds acid from18333
68371L-fucose-builds acid from18287
68371D-fucose-builds acid from28847
68371D-tagatose-builds acid from16443
68371D-lyxose-builds acid from62318
68371turanose-builds acid from32528
68371gentiobiose-builds acid from28066
68371xylitol-builds acid from17151
68371glycogen-builds acid from28087
68371starch-builds acid from28017
68371raffinose-builds acid from16634
68371melezitose-builds acid from6731
68371inulin-builds acid from15443
68371trehalose-builds acid from27082
68371sucrose-builds acid from17992
68371melibiose-builds acid from28053
68371maltose-builds acid from17306
68371cellobiose-builds acid from17057
68371salicin-builds acid from17814
68371esculin-builds acid from4853
68371arbutin-builds acid from18305
68371amygdalin-builds acid from27613
68371methyl alpha-D-glucopyranoside-builds acid from320061
68371methyl alpha-D-mannoside-builds acid from43943
68371D-sorbitol-builds acid from17924
68371D-mannitol-builds acid from16899
68371myo-inositol-builds acid from17268
68371galactitol-builds acid from16813
68371L-rhamnose-builds acid from62345
68371L-sorbose-builds acid from17266
68371methyl beta-D-xylopyranoside-builds acid from74863
68371ribitol-builds acid from15963
68371L-xylose-builds acid from65328
68371D-xylose-builds acid from65327
68371D-ribose-builds acid from16988
68371L-arabinose-builds acid from30849
68371D-arabinose-builds acid from17108
68371erythritol-builds acid from17113
68371glycerol-builds acid from17754
121545nitrate-reduction17632
121545nitrite-reduction16301
121545nitrate+respiration17632

metabolite tests

  • @ref: 121545
  • Chebi-ID: 15688
  • metabolite: acetoin
  • voges-proskauer-test: -

enzymes

@refvalueactivityec
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382beta-glucosidase-3.2.1.21
68382alpha-glucosidase-3.2.1.20
68382beta-glucuronidase-3.2.1.31
68382beta-galactosidase+3.2.1.23
68382alpha-galactosidase-3.2.1.22
68382naphthol-AS-BI-phosphohydrolase+
68382acid phosphatase+3.1.3.2
68382alpha-chymotrypsin-3.4.21.1
68382trypsin-3.4.21.4
68382cystine arylamidase-3.4.11.3
68382valine arylamidase+
68382leucine arylamidase+3.4.11.1
68382lipase (C 14)-
68382esterase lipase (C 8)-
68382esterase (C 4)-
68382alkaline phosphatase-3.1.3.1
121545oxidase-
121545alcohol dehydrogenase-1.1.1.1
121545catalase-1.11.1.6
121545lysine decarboxylase-4.1.1.18
121545ornithine decarboxylase-4.1.1.17

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
121545-----++---++-+------

API 50CHac

@refQGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
8667----------++++--------+------+--------------------
121545---------+/-+/--+/---------+/-------+/---------------------

Isolation, sampling and environmental information

isolation

@refsample type
8667pasteurized milk
52355Milk,pasteurized
67770Pasteurized milk
121545Food, Pasteurized milk

isolation source categories

Cat1Cat2Cat3
#Engineered#Food production#Dairy product
#Engineered#Treatment#Preserved
#Engineered#Treatment#Sterilized (Desinfected)

taxonmaps

  • @ref: 69479
  • File name: preview.99_113.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_45;96_88;97_93;98_102;99_113&stattab=map
  • Last taxonomy: Lactobacillaceae
  • 16S sequence: LC065035
  • Sequence Identity:
  • Total samples: 44444
  • soil counts: 1801
  • aquatic counts: 2397
  • animal counts: 39363
  • plant counts: 883

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
86671Risk group (German classification)
1215451Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Lactobacillus paracasei gene, 16S-23S rRNA intergenic spacer region and 23S rRNA gene 5' flanking region, partial sequenceAB035487670ena1597
20218Lactobacillus paracasei subsp. tolerans genes for 16S rRNA, 16S-23S internal transcribed spacer, tRNA-Ile, tRNA-Ala, 23S rRNA, partial and complete sequence, strain:JCM 1171AB237502572ena113557
20218Lactobacillus paracasei subsp. tolerans genes for 16S rRNA, 16S-23S internal transcribed spacer, 23S rRNA, partial sequence, strain:JCM 1171AB237503364ena113557
20218Lactobacillus paracasei subsp. tolerans gene for 16S rRNA, partial sequence, strain: JCM 1171AB289229623ena113557
20218Lactobacillus casei gene for 16S rRNAD165501520ena1582
20218Lactobacillus paracasei subsp. tolerans gene for 16S rRNA, partial sequenceAB1819501497ena113557
20218Lactobacillus paracasei subsp. tolerans gene for 16S rRNA, partial sequence, strain: NBRC 15906AB6809951497ena113557
67770Lactobacillus paracasei subsp. tolerans gene for 16S ribosomal RNA, partial sequence, strain: JCM 1171LC0650351467ena113557

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Lacticaseibacillus paracasei subsp. tolerans NBRC 15906GCA_007989185contigncbi113557
66792Lactobacillus paracasei subsp. tolerans DSM 202581423830.3wgspatric1423830
66792Lactobacillus paracasei subsp. tolerans strain NBRC 15906113557.4wgspatric113557
66792Lacticaseibacillus paracasei tolerans DSM 202582657245104draftimg1423830
67770Lacticaseibacillus paracasei subsp. tolerans DSM 20258GCA_001436485scaffoldncbi1423830

GC content

@refGC-contentmethod
866746.9
6777046.4genome sequence analysis

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
motileno93.97no
gram-positiveyes96.817no
anaerobicno93.681no
halophileyes83.756no
spore-formingno92.784no
glucose-utilyes89.679no
thermophileno98.661yes
aerobicno96.26no
flagellatedno97.549no
glucose-fermentyes91.022no

External links

@ref: 8667

culture collection no.: DSM 20258, ATCC 25599, NCIB 9709, CCUG 34829, JCM 1171, BCRC 14628, CCM 7092, CECT 4175, CIP 102994, CIP 103024, IFO 15906, KCTC 3074, LMG 9191, NBRC 15906, NCFB 2742, NCIMB 9709, NRIC 1045, NCDO 2742, NCFB 2774

straininfo link

  • @ref: 75908
  • straininfo: 3342

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
8667Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 20258)https://www.dsmz.de/collection/catalogue/details/culture/DSM-20258
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
38115Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/14775
52355Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 34829)https://www.ccug.se/strain?id=34829
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68371Automatically annotated from API 50CH acid
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
75908Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID3342.1StrainInfo: A central database for resolving microbial strain identifiers
121545Curators of the CIPCollection of Institut Pasteur (CIP 103024)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20103024