Name: Halomonas utahensis 65
Creator: BacDive
License: https://creativecommons.org/licenses/by/4.0/

Strain identifier

BacDive ID: 6025

Type strain:

Species: Halomonas utahensis

Strain Designation: 65

Culture col. no.: DSM 3051, III 65, IAM 14440, JCM 21223, ATCC 49240, CIP 105504, NBRC 102410

Strain history: CIP <- 1998, DSMZ <- C. Fendrich, TU Braunschweig

NCBI tax ID(s): 86177 (species)

SI-ID 42376

ATCC 49240, DSM 3051, CIP 105504, IAM 14440, NCIMB 12993, NBRC 102410, JCM 21223, LMG 23828

Special Collections

Section

Halomonas utahensis 65 is a Gram-negative, motile, rod-shaped bacterium that was isolated from hypersaline surface water.

Gram-negative
motile
rod-shaped
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Pseudomonadota*
	Class	*Gammaproteobacteria*
	Order	*Oceanospirillales*
	Family	*Halomonadaceae*
	Genus	*Halomonas*
	Species	**Halomonas utahensis**
	Full Scientific Name (LPSN)	Halomonas utahensis Sorokin and Tindall 2006

	Synonym	"Vreelandella* utahensis"*
	Synonym	Pseudomonas halophila

Information on morphological and physiological properties Morphology

[Ref.: #123798]	Gram stain	negative
[Ref.: #69480]	Gram stain	negative Confidence in %98.75

[Ref.: #123798]	Cell shape	rod-shaped

[Ref.: #123798]	Motility	yes

Information on culture and growth conditions Culture and growth conditions

[Ref.: #1286]

Culture medium

MEDIUM 514 PLUS ADDITIONAL SALT (DSMZ Medium 514b)

[Ref.: #1286]

Culture medium growth

[Ref.: #1286]

Culture medium link

Medium recipe at MediaDive

[Ref.: #1286]

Culture medium composition

expand / minimize

[Ref.: #1286]

Culture medium

HALOVIBRIO VARIABILIS MEDIUM (DSMZ Medium 598)

[Ref.: #1286]

Culture medium growth

[Ref.: #1286]

Culture medium link

Medium recipe at MediaDive

[Ref.: #1286]

Culture medium composition

expand / minimize

[Ref.: #40186]

Culture medium

MEDIUM 286 - for Halomonas variabilis

[Ref.: #40186]

Culture medium growth

[Ref.: #40186]

Culture medium composition

expand / minimize

[Ref.: #123798]

Culture medium

CIP Medium 286

[Ref.: #123798]

Culture medium growth

[Ref.: #123798]

Culture medium link

Medium recipe provided by DSMZ

	Kind of temperature		Temperature
[Ref.: #1286]		growth	30 °C
[Ref.: #40186]		growth	30 °C
[Ref.: #67770]		growth	30 °C
[Ref.: #123798]		growth	15-37 °C
[Ref.: #123798]	no	growth	5 °C
[Ref.: #123798]	no	growth	45 °C

Information on physiology and metabolism Physiology and metabolism

	Salt	Tested relation		Salt conc.
[Ref.: #123798]	NaCl		growth	4-10	%
[Ref.: #123798]	NaCl	no	growth	0	%
[Ref.: #123798]	NaCl	no	growth	2	%

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #123798]	esculin ChEBI	-	hydrolysis
[Ref.: #123798]	hippurate ChEBI	-	hydrolysis
[Ref.: #123798]	malonate ChEBI	-	assimilation
[Ref.: #123798]	nitrate ChEBI	-	builds gas from
[Ref.: #123798]	nitrate ChEBI	-	reduction
[Ref.: #123798]	nitrite ChEBI	-	builds gas from
[Ref.: #123798]	nitrite ChEBI	-	reduction

	Metabolite production	Metabolite	Production
[Ref.: #123798]		indole ChEBI	no

	Physiological tests	Metabolite	Voges-Proskauer-test	Methylred-test
[Ref.: #123798]		acetoin ChEBI	-
[Ref.: #123798]		glucose ChEBI		-

	Enzyme	Enzyme activity	EC number
[Ref.: #68382]	acid phosphatase	-	3.1.3.2	* from API zym
[Ref.: #123798]	alcohol dehydrogenase	-	1.1.1.1
[Ref.: #68382]	alkaline phosphatase	-	3.1.3.1	* from API zym
[Ref.: #68382]	alpha-chymotrypsin	-	3.4.21.1	* from API zym
[Ref.: #68382]	alpha-fucosidase	-	3.2.1.51	* from API zym
[Ref.: #68382]	alpha-galactosidase	-	3.2.1.22	* from API zym
[Ref.: #68382]	alpha-glucosidase	-	3.2.1.20	* from API zym
[Ref.: #68382]	alpha-mannosidase	-	3.2.1.24	* from API zym
[Ref.: #123798]	amylase	-
[Ref.: #123798]	beta-galactosidase	+	3.2.1.23
[Ref.: #68382]	beta-galactosidase	-	3.2.1.23	* from API zym
[Ref.: #68382]	beta-glucosidase	-	3.2.1.21	* from API zym
[Ref.: #68382]	beta-glucuronidase	-	3.2.1.31	* from API zym
[Ref.: #123798]	caseinase	-	3.4.21.50
[Ref.: #123798]	catalase	+	1.11.1.6
[Ref.: #68382]	cystine arylamidase	-	3.4.11.3	* from API zym
[Ref.: #68382]	esterase (C 4)	+		* from API zym
[Ref.: #68382]	esterase lipase (C 8)	+		* from API zym
[Ref.: #123798]	gamma-glutamyltransferase	+	2.3.2.2
[Ref.: #123798]	gelatinase	-
[Ref.: #123798]	lecithinase	-
[Ref.: #68382]	leucine arylamidase	+	3.4.11.1	* from API zym
[Ref.: #123798]	lipase	-
[Ref.: #68382]	lipase (C 14)	-		* from API zym
[Ref.: #123798]	lysine decarboxylase	-	4.1.1.18
[Ref.: #68382]	N-acetyl-beta-glucosaminidase	-	3.2.1.52	* from API zym
[Ref.: #68382]	naphthol-AS-BI-phosphohydrolase	+		* from API zym
[Ref.: #123798]	ornithine decarboxylase	-	4.1.1.17
[Ref.: #123798]	oxidase	+
[Ref.: #123798]	phenylalanine ammonia-lyase	-	4.3.1.24
[Ref.: #68382]	trypsin	-	3.4.21.4	* from API zym
[Ref.: #123798]	tryptophan deaminase	-
[Ref.: #123798]	tween esterase	-
[Ref.: #123798]	urease	-	3.5.1.5
[Ref.: #68382]	valine arylamidase	-		* from API zym
	Only first 10 entries are displayed. Click here to see all.

API® Biotype100:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	ControlQ	D-glucose as carbon sourceGLU	D-fructose as carbon sourceFRU	D-galactose as carbon sourceGAL	D-trehalose as carbon sourceTRE	D-mannose as carbon sourceMNE	L-sorbose as carbon sourceSBE	D-melibiose as carbon sourceMEL	sucrose as carbon sourceSAC	D-raffinose as carbon sourceRAF	maltotriose as carbon sourceMTE	maltose as carbon sourceMAL	lactose as carbon sourceLAC	lactulose as carbon sourceLTE	1-O-methyl-beta-galactopyranoside as carbon sourceMbGa	1-O-methyl-alpha-galactopyranoside as carbon sourceMaGa	D-cellobiose as carbon sourceCEL	gentiobiose as carbon sourceGEN	1-O-methyl-beta-D-glucopyranoside as carbon sourceMbGu	esculin as carbon sourceESC	D-ribose as carbon sourceRIB	L-arabinose as carbon sourceARA	D-xylose as carbon sourceXYL	palatinose as carbon sourcePLE	L-rhamnose as carbon sourceRHA	L-fucose as carbon sourceFUC	D-melezitose as carbon sourceMLZ	D-arabitol as carbon sourceDARL	L-arabitol as carbon sourceLARL	xylitol as carbon sourceXLT	dulcitol as carbon sourceDUL	D-tagatose as carbon sourceTAG	glycerol as carbon sourceGLY	myo-inositol as carbon sourceINO	D-mannitol as carbon sourceMAN	maltitol as carbon sourceMTL	D-turanose as carbon sourceTUR	D-sorbitol as carbon sourceSOR	adonitol as carbon sourceADO	hydroxyquinoline-beta-glucuronide as carbon sourceHBG	D-lyxose as carbon sourceLYX	i-erythritol as carbon sourceERY	1-O-methyl-alpha-D-glucoside as carbon sourceMDG	3-O-methyl-D-glucose as carbon source3MDG	D-saccharate as carbon sourceSAT	mucate as carbon sourceMUC	L-tartrate as carbon sourceLTAT	D-tartrate as carbon sourceDTAT	meso-tartrate as carbon sourceMTAT	D-malate as carbon sourceDMLT	L-malate as carbon sourceLMLT	cis-aconitate as carbon sourceCATE	trans-aconitate as carbon sourceTATE	tricarballylate as carbon sourceTTE	citrate as carbon sourceCIT	D-glucuronate as carbon sourceGRT	D-galacturonate as carbon sourceGAT	2-ketogluconate as carbon source2KG	5-ketogluconate as carbon source5KG	tryptophan as carbon sourceTRY	N-acetyl-D-glucosamine as carbon sourceNAG	D-gluconate as carbon sourceGNT	phenylacetate as carbon sourcePAC	protocatechuate as carbon sourcePAT	4-hydroxybenzoate as carbon sourcepOBE	quinate as carbon sourceQAT	gentisate as carbon sourceGTE	3-hydroxybenzoate as carbon sourcemOBE	benzoate as carbon sourceBAT	3-phenylpropionate as carbon sourcePPAT	m-coumarate as carbon sourceCMT	trigonelline as carbon sourceTGE	betaine as carbon sourceBET	putrescine as carbon sourcePCE	4-aminobutyrate as carbon sourceABT	histamine as carbon sourceHIN	DL-lactate as carbon sourceLAT	caprate as carbon sourceCAP	caprylate as carbon sourceCYT	L-histidine as carbon sourceHIS	succinate as carbon sourceSUC	fumarate as carbon sourceFUM	glutarate as carbon sourceGRE	DL-glycerate as carbon sourceGYT	5-aminovalerate as carbon sourceAVT	ethanolamine as carbon sourceETN	tryptamine as carbon sourceTTN	D-glucosamine as carbon sourceGLN	itaconate as carbon sourceITA	3-hydroxybutyrate as carbon source3OBU	L-aspartate as carbon sourceAPT	L-glutamate as carbon sourceGTT	L-proline as carbon sourcePRO	D-alanine as carbon sourceDALA	L-alanine as carbon sourceLALA	L-serine as carbon sourceSER	malonate as carbon sourceMNT	propionate as carbon sourcePROP	L-tyrosine as carbon sourceTYR	2-ketoglutarate as carbon source2KT
[Ref.: #123798]	1186	not determinedn.d.	+	+	+	+	-	-	-	+	-	+	+	-	-	-	-	+	+	+	+	-	+	+	-	-	-	-	+	-	-	-	-	+	-	+	-	+	+	-	+	-	-	-	-	+	-	-	-	-	+	+	+	-	-	+	-	-	-	-	-	-	+	-	-	-	-	-	-	-	-	-	-	+	-	+	-	+	-	-	-	+	+	-	-	-	-	-	-	-	+	-	+	+	+	-	-	-	+	-	-

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #123798]	8753	-	-	+	+	-	+	-	-	-	-	-	+	-	-	-	-	-	-	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #1286]	Sample type/isolated from	hypersaline surface water
[Ref.: #1286]	Geographic location (country and/or sea, region)	Utah, Great salt lake of the north Arm
[Ref.: #1286]	Country	USA
[Ref.: #1286]	Country ISO 3 Code	USA
[Ref.: #1286]	Continent	North America

[Ref.: #67770]	Sample type/isolated from	Surface water from the North Arm of Great Salt Lake
[Ref.: #67770]	Geographic location (country and/or sea, region)	UT
[Ref.: #67770]	Country	USA
[Ref.: #67770]	Country ISO 3 Code	USA
[Ref.: #67770]	Continent	North America

[Ref.: #123798]	Sample type/isolated from	Environment, Hypersaline surface water
[Ref.: #123798]	Country	Germany
[Ref.: #123798]	Country ISO 3 Code	DEU
[Ref.: #123798]	Continent	Europe
* marker position based on {}

Isolation sources categories

#Environmental	#Aquatic	#Surface water
#Condition	#Saline	-

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence AJ306893 (>99% sequence identity) for Halomonas from Microbeatlas

Aquatic Samples	3268
Soil Samples	321
Animal Samples	696
Plant Samples	83
Total Samples	4368

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #1286]	Biosafety level	1	Risk group (German classification) According to TRBA 466
[Ref.: #123798]	Biosafety level	1	Risk group (French classification)

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #1286]	Halomonas variabilis partial 16S rRNA gene, strain DSM 3051	AJ306893	1528	GenBank ENA	31910 tax ID
[Ref.: #20218]	Halovibrio variabilis (DSM 3051) 16S ribosomal RNA (16S rRNA) gene	M93357	1470	GenBank ENA	31910 tax ID	*
[Ref.: #20218]	Halomonas variabilis genes for 16S ribosomal RNA, internal transcribed spacer 1 and 23S ribosomal RNA, partial and complete sequence, clone: 873	AB367223	873	GenBank ENA	31910 tax ID	*
[Ref.: #20218]	Halomonas variabilis genes for 16S ribosomal RNA, internal transcribed spacer 1 and 23S ribosomal RNA, partial and complete sequence, clone: 1387	AB367224	1387	GenBank ENA	31910 tax ID	*
[Ref.: #20218]	Halomonas variabilis gene for 16S rRNA, partial sequence, strain: NBRC 102410	AB681766	1458	GenBank ENA	31910 tax ID	*

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Halovibrio variabilis NBRC 102410	GCA_007991175	contig	GenBank ENA	31910 tax ID	*
[Ref.: #66792]	Halomonas utahensis DSM 3051	GCA_019903425	scaffold	GenBank ENA	86177 tax ID	*
[Ref.: #66792]	Halomonas utahensis strain DSM 3051	86177.9	wgs	PATRIC	86177 tax ID	*
[Ref.: #66792]	Halovibrio variabilis strain NBRC 102410	31910.4	wgs	PATRIC	31910 tax ID	*

[Ref.: #1286]	GC-content	58 mol%	thermal denaturation, midpoint method (Tm)
[Ref.: #67770]	GC-content	61 mol%

Data predicted using genome information as a basis Genome-based predictions

	Trait	Model	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Halomonas utahensis DSM 3051 NCBI: GCA_019903425.1
[Ref.: #69480]	gram-positive	gram-positiveⓘ	no	98.75	no
[Ref.: #69480]	anaerobic	anaerobicⓘ	no	92.804	no
[Ref.: #69480]	aerobic	aerobicⓘ	yes	73.597	no
[Ref.: #69480]	spore-forming	spore-formingⓘ	no	86.567	no
[Ref.: #69480]	thermophilic	thermophileⓘ	no	94.742	no
[Ref.: #69480]	flagellated	motile2+ⓘ	yes	87.99	no

Availability in culture collections External links

[Ref.: #1286]

Culture collection no.

DSM 3051, III 65, IAM 14440, JCM 21223, ATCC 49240, CIP 105504, NBRC 102410

[Ref.: #75511]

SI-ID 42376

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	The designated type strain of Pseudomonas halophila Fendrich 1989 is DSM 3051, the designated type strain of Halovibrio variabilis Fendrich 1989 is DSM 3050, the new name Halomonas utahensis (Fendrich 1989) Sorokin and Tindall 2006 is created for the species represented by DSM 3051 when treated as a member of the genus Halomonas, the combination Halomonas variabilis (Fendrich 1989) Dobson and Franzmann 1996 is rejected, and the combination Halovibrio denitrificans Sorokin et al. 2006 is validly published with an emendation of the description of the genus Halovibrio Fendrich 1989 emend. Sorokin et al. 2006. Opinion 93. Judicial Commission of the International Committee on Systematics of Prokaryotes.	Tindall BJ	Int J Syst Evol Microbiol	10.1099/ijs.0.069195-0	2014	*
Phylogeny	The status of the genus name Halovibrio Fendrich 1989 and the identity of the strains Pseudomonas halophila DSM 3050 and Halomonas variabilis DSM 3051. Request for an opinion.	Sorokin DY, Tindall BJ	Int J Syst Evol Microbiol	10.1099/ijs.0.63965-0	2006	*
Phylogeny	Extremely halophilic denitrifying bacteria from hypersaline inland lakes, Halovibrio denitrificans sp. nov. and Halospina denitrificans gen. nov., sp. nov., and evidence that the genus name Halovibrio Fendrich 1989 with the type species Halovibrio variabilis should be associated with DSM 3050.	Sorokin DY, Tourova TP, Galinski EA, Belloch C, Tindall BJ	Int J Syst Evol Microbiol	10.1099/ijs.0.63964-0	2006	*

References

#1286

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 3051

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#20218

Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )

#40186 ; Curators of the CIP;

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#68382

Automatically annotated from API zym .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#75511

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID42376.1 )

#123798 Collection of Institut Pasteur ; Curators of the CIP; CIP 105504
* These data were automatically processed and therefore are not curated

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Halomonas utahensis DSM 3051

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