Haloferax lucentense (DSM 14919, CCM 7023, CECT 5871)

Name: Haloferax lucentense (DSM 14919, CCM 7023, CECT 5871)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 5935

Type strain

Strain Designation Aa 2.2

Culture col. no. DSM 14919 CCM 7023 CECT 5871 CIP 107410 JCM 9276 5 more

NCBI tax ID(s) 1230452 2246

Special Collections DSMZ JCM CIP

History DSMZ <- A. Ventosa, Univ. of Sevilla; Aa 2.2 <- M. L. Dyall-Smith <- F. Rodríguez-Valera <- M. Torreblanca CIP <- 2002, A. Ventosa, Sevilla Univ., Sevilla, Spain: strain Aa 2.2 JCM <-- S. D. Nuttal <-- M. Torreblanca Aa 2.2. CIP <- 2002, A. Ventosa, Sevilla Univ., Sevilla, Spain: strain Aa 2.2

Links

Haloferax lucentense Aa 2.2 is a Gram-negative, ovoid-shaped archaeon that was isolated from water of a saltern.

Gram-negative ovoid-shaped genome sequence 16S sequence Archaea

Name and taxonomic classification

SI-ID 65751

JCM 9276,AS 1.2151,CCM 7023,CECT 5871,CIP 107410,DSM 14919,NCIMB 13854,CGMCC 1.2151

@ref 20215

Last LPSN update 2026-05-29 08:26:17

Domain Archaea

Phylum Methanobacteriota

Class Halobacteria

Order Halobacteriales

Family Haloferacaceae

Genus Haloferax

Species Haloferax lucentense

Full scientific name Haloferax lucentense corrig. Gutiérrez et al. 2004

Synonyms (6)

Halobacterium volcanii
Haloferax lucentensis
"Haloferax alicantei"
Haloferax alexandrinus
Haloferax volcanii
Haloferax alexandrinum

Morphology

Cell morphology

@ref	Gram stain	Cell shape	Motility
120661	negative	ovoid-shaped

Colony morphology

120661

Hemolysis ability0

Culture and growth conditions

Culture medium

@ref	Name	Medium link	Composition
5606	HALOBACTERIA MEDIUM (DSMZ Medium 372)	Medium recipe at MediaDive	Name: HALOBACTERIA MEDIUM (DSMZ Medium 372) Composition: NaCl 200.0 g/l MgSO4 x 7 H2O 20.0 g/l Agar 20.0 g/l Yeast extract 5.0 g/l Casamino acids 5.0 g/l Na3-citrate 3.0 g/l KCl 2.0 g/l Na glutamate 1.0 g/l FeCl2 x 4 H2O 0.036 g/l MnCl2 x 4 H2O 0.00036 g/l Distilled water
33788	MEDIUM 204 - for Halorubrum, Marinococcus and Natrialba		Distilled water make up to (1000.000 ml);Sodium chloride (200.000 g);Potassium chloride (2.000 g);ManganeseII chloride tetrahydrate (0.360 mg);Magnesium sulphate heptahydrate (20.000 g);Agar (20.000 g);Yeast extract (5.000 g);Ferrous chloride tetrahydrate
120661	CIP Medium 204	Medium recipe at CIP

Culture temp

@ref	Growth	Type	Temperature (°C)
5606	positive	growth	37
33788	positive	growth	37
67770	positive	growth	37
120661	positive	growth	37-41
120661	negative	growth	5
120661	negative	growth	15
120661	negative	growth	30
120661	negative	growth	45

Physiology and metabolism

Spore formation

@ref	Spore formation	Confidence
125439		98.808

Halophily

@ref	Salt	Tested relation	Concentration
120661	NaCl	growth	0 %
120661	NaCl	growth	2 %
120661	NaCl	growth	4 %
120661	NaCl	growth	6 %
120661	NaCl	growth	8 %
120661	NaCl	growth	10 %

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
120661	16947 ChEBI	citrate	-	carbon source
120661	4853 ChEBI	esculin	-	hydrolysis
120661	606565 ChEBI	hippurate	-	hydrolysis
120661	15792 ChEBI	malonate	-	assimilation
120661	17632 ChEBI	nitrate	-	reduction
120661	16301 ChEBI	nitrite	-	reduction

Metabolite production

@ref	Chebi-ID	Metabolite	Production
120661	35581 ChEBI	indole

Metabolite tests

@ref	Chebi-ID	Metabolite	Voges-proskauer-test
120661	15688 ChEBI	acetoin	+

Enzymes

@ref	Value	Activity	Ec
68382	acid phosphatase	-	3.1.3.2	from API zym
120661	alcohol dehydrogenase	-	1.1.1.1
68382	alkaline phosphatase	-	3.1.3.1	from API zym
68382	alpha-chymotrypsin	-	3.4.21.1	from API zym
68382	alpha-fucosidase	-	3.2.1.51	from API zym
68382	alpha-galactosidase	-	3.2.1.22	from API zym
68382	alpha-glucosidase	-	3.2.1.20	from API zym
68382	alpha-mannosidase	-	3.2.1.24	from API zym
68382	beta-galactosidase	-	3.2.1.23	from API zym
120661	beta-galactosidase	-	3.2.1.23
68382	beta-glucosidase	-	3.2.1.21	from API zym
68382	beta-glucuronidase	-	3.2.1.31	from API zym
120661	catalase	+	1.11.1.6
68382	cystine arylamidase	-	3.4.11.3	from API zym
68382	esterase (C 4)	+		from API zym
68382	esterase lipase (C 8)	+		from API zym
120661	gamma-glutamyltransferase	-	2.3.2.2
120661	gelatinase	-
68382	leucine arylamidase	+	3.4.11.1	from API zym
68382	lipase (C 14)	-		from API zym
120661	lysine decarboxylase	-	4.1.1.18
68382	N-acetyl-beta-glucosaminidase	-	3.2.1.52	from API zym
68382	naphthol-AS-BI-phosphohydrolase	-		from API zym
120661	ornithine decarboxylase	-	4.1.1.17
120661	oxidase	-
120661	phenylalanine ammonia-lyase	-	4.3.1.24
68382	trypsin	-	3.4.21.4	from API zym
120661	tryptophan deaminase	-
120661	urease	-	3.5.1.5
68382	valine arylamidase	-		from API zym

API zym

@ref	Control	Alkaline phosphatase	Esterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	Lipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	Trypsin	alpha- Chymotrypsin	Acid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	alpha- Galactosidase	beta- Galactosidase	beta- Glucuronidase	alpha- Glucosidase	beta- Glucosidase	N-acetyl-beta- glucosaminidase	alpha- Mannosidase	alpha- Fucosidase
120661	-	-	+	+	-	+	-	-	-	-	-	-	-	-	-	-	-	-	-	-

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2
#Environmental	#Aquatic
#Condition	#Saline

Isolation

@ref	Sample type	Geographic location	Country	Country ISO 3 Code	Continent	Isolation date
5606	water of a saltern	Alicante	Spain	ESP	Europe
67770	Saltern in Alicante		Spain	ESP	Europe
120661	Environment, Water of a saltern	Alicante	Spain	ESP	Europe	1986

Taxonmaps

69479

Global distribution of 16S sequence AB663378 (>99% sequence identity) for Haloferax from Microbeatlas

Aquatic Samples	7
Soil Samples	1
Animal Samples	0
Plant Samples	0
Total Samples	8

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
5606	1	Risk group (German classification) According to TRBA 466
120661	1	Risk group (French classification) According to TRBA 466

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
67770	ASM33679v1 assembly for Haloferax volcanii DSM 14919	contig	GCA_000336795	1230452.3	2554235494	1230452	70.75

16S sequences

@ref	Description	Accession	Length	NCBI tax ID
20218	Haloferax lucentensis gene for 16S rRNA	AB081732	1473	2254
5606	Haloferax lucentense gene for 16S rRNA, complete sequence, strain: JCM 9276	AB663378	1473	2254
67770	Haloferax lucentense DSM 14919 strain Aa 2.2 16S ribosomal RNA genes, partial sequence	AH003665	1387	1230452
124043	Haloferax lucentense strain JCM 9276 16S ribosomal RNA gene, partial sequence.	MH062946	1220	2246

GC content

@ref	GC-content (mol%)	Method
67770	64.5	thermal denaturation, midpoint method (Tm)

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: ASM33679v1 assembly for Haloferax volcanii DSM 14919
NCBI: GCA_000336795

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	oxygen_tolerance	BacteriaNetⓘ	obligate anaerobe	73.69	no
125439	gram_stain	BacteriaNetⓘ	negative	82.55	no
125439	motility	BacteriaNetⓘ	yes	63.34	no
125439	spore_formation	BacteriaNetⓘ	no	98.81	no

Literature

Topic	Title	Authors	Journal	DOI	Year
	Evolutionary insights into provirus-encoded CRISPR-Cas systems in halophilic archaea.	Naki D, Gophna U.	Microlife	10.1093/femsml/uqaf033	2025
Phylogeny	Characterization by polyphasic approach of some indigenous halophilic archaea of Djelfa's rock salt "Hadjr el Meelh", Algeria.	Beddal A, Boutaiba S, Laassami A, Hamaidi F, Enache M.	Iran J Microbiol	10.18502/ijm.v14i4.10240	2022
Metabolism	Dihydroxyacetone metabolism in Haloferax volcanii.	Ouellette M, Makkay AM, Papke RT.	Front Microbiol	10.3389/fmicb.2013.00376	2013
	Comparative Analysis of Surface Layer Glycoproteins and Genes Involved in Protein Glycosylation in the Genus Haloferax.	Shalev Y, Soucy SM, Papke RT, Gogarten JP, Eichler J, Gophna U.	Genes (Basel)	10.3390/genes9030172	2018
	The Adaptive Immune System of Haloferax volcanii.	Maier LK, Dyall-Smith M, Marchfelder A.	Life (Basel)	10.3390/life5010521	2015
Enzymology	Biochemical and Structural Insights into a Novel Thermostable beta-1,3-Galactosidase from Marinomonas sp. BSi20414.	Ding H, Zeng Q, Zhou L, Yu Y, Chen B.	Mar Drugs	10.3390/md15010013	2017
	Antioxidant, Antimicrobial, and Bioactive Potential of Two New Haloarchaeal Strains Isolated from Odiel Salterns (Southwest Spain).	Gomez-Villegas P, Vigara J, Vila M, Varela J, Barreira L, Leon R.	Biology (Basel)	10.3390/biology9090298	2020
Phylogeny	Genomic insights on carotenoid synthesis by extremely halophilic archaea Haloarcula rubripromontorii BS2, Haloferax lucentense BBK2 and Halogeometricum borinquense E3 isolated from the solar salterns of India.	Nagar DN, Mani K, Braganca JM.	Sci Rep	10.1038/s41598-024-70149-4	2024
	Halocin H4 is activated through cleavage by halolysin HlyR4.	Chen S, Dai Y, Ke J, Luo Y, Wang C, Hao Y, Zhang A, Han J, Xiang H.	Appl Environ Microbiol	10.1128/aem.02284-23	2024
Metabolism	Biology and survival of extremely halophilic archaeon Haloarcula marismortui RR12 isolated from Mumbai salterns, India in response to salinity stress.	Thombre RS, Shinde VD, Oke RS, Dhar SK, Shouche YS.	Sci Rep	10.1038/srep25642	2016
	Cellular and Genomic Properties of Haloferax gibbonsii LR2-5, the Host of Euryarchaeal Virus HFTV1.	Tittes C, Schwarzer S, Pfeiffer F, Dyall-Smith M, Rodriguez-Franco M, Oksanen HM, Quax TEF.	Front Microbiol	10.3389/fmicb.2021.625599	2021
Phylogeny	PH1: an archaeovirus of Haloarcula hispanica related to SH1 and HHIV-2.	Porter K, Tang SL, Chen CP, Chiang PW, Hong MJ, Dyall-Smith M.	Archaea	10.1155/2013/456318	2013
Genetics	Genome sequence and description of Haloferax massiliense sp. nov., a new halophilic archaeon isolated from the human gut.	Khelaifia S, Caputo A, Andrieu C, Cadoret F, Armstrong N, Michelle C, Lagier JC, Djossou F, Fournier PE, Raoult D.	Extremophiles	10.1007/s00792-018-1011-1	2018
Phylogeny	Taxonomic characterization of Haloferax sp. (" H. alicantei") strain Aa 2.2: description of Haloferax lucentensis sp. nov.	Gutierrez MC, Kamekura M, Holmes ML, Dyall-Smith ML, Ventosa A	Extremophiles	10.1007/s00792-002-0282-7	2002

References

#5606	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 14919
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#20218	Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )
#33788	; Curators of the CIP;
#67770	Japan Collection of Microorganism (JCM) ; Curators of the JCM;
#68382	Automatically annotated from API zym .
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#120661	Collection of Institut Pasteur ; Curators of the CIP; CIP 107410
#124043	Isabel Schober, Julia Koblitz: Data extracted from sequence databases, automatically matched based on designation and taxonomy .
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Haloferax lucentense (DSM 14919, CCM 7023, CECT 5871)

Name and taxonomic classification

Morphology

Cell morphology

Colony morphology

Culture and growth conditions

Culture medium

Culture temp

Physiology and metabolism

Spore formation

Halophily

Metabolite utilization

Metabolite production

Metabolite tests

Enzymes

API zym

Isolation, sampling and environmental information

Isolation source categories

Isolation

Taxonmaps

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_000336795

16S sequences

GC content

Genome-based predictions

Predictions

Literature

Literature

References

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