Strain identifier
BacDive ID: 5935
Type strain:
Species: Haloferax lucentense
Strain Designation: Aa 2.2
Strain history: CIP <- 2002, A. Ventosa, Sevilla Univ., Sevilla, Spain: strain Aa 2.2
NCBI tax ID(s): 1230452 (strain), 2254 (species)
General
@ref: 5606
BacDive-ID: 5935
DSM-Number: 14919
keywords: genome sequence, 16S sequence, Archaea, mesophilic, Gram-negative, ovoid-shaped
description: Haloferax lucentense Aa 2.2 is a mesophilic, Gram-negative, ovoid-shaped archaeon that was isolated from water of a saltern.
NCBI tax id
NCBI tax id | Matching level |
---|---|
2254 | species |
1230452 | strain |
strain history
@ref | history |
---|---|
5606 | <- A. Ventosa, Univ. of Sevilla; Aa 2.2 <- M. L. Dyall-Smith <- F. Rodríguez-Valera <- M. Torreblanca |
33788 | 2002, A. Ventosa, Sevilla Univ., Sevilla, Spain: strain Aa 2.2 |
67770 | S. D. Nuttal <-- M. Torreblanca Aa 2.2. |
120661 | CIP <- 2002, A. Ventosa, Sevilla Univ., Sevilla, Spain: strain Aa 2.2 |
doi: 10.13145/bacdive5935.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/archaea
- keyword: phylum/methanobacteriota
- domain: Archaea
- phylum: Methanobacteriota
- class: Halobacteria
- order: Haloferacales
- family: Haloferacaceae
- genus: Haloferax
- species: Haloferax lucentense
- full scientific name: Haloferax lucentense corrig. Gutierrez et al. 2004
synonyms
@ref synonym 20215 Haloferax alicantei 20215 Haloferax lucentensis
@ref: 5606
domain: Archaea
phylum: Euryarchaeota
class: Halobacteria
order: Halobacteriales
family: Haloferacaceae
genus: Haloferax
species: Haloferax lucentense
full scientific name: Haloferax lucentense Gutierrez et al. 2004
strain designation: Aa 2.2
type strain: yes
Morphology
cell morphology
- @ref: 120661
- gram stain: negative
- cell shape: ovoid-shaped
- motility: no
colony morphology
- @ref: 120661
Culture and growth conditions
culture medium
@ref | name | growth | link | composition |
---|---|---|---|---|
5606 | HALOBACTERIA MEDIUM (DSMZ Medium 372) | yes | https://mediadive.dsmz.de/medium/372 | Name: HALOBACTERIA MEDIUM (DSMZ Medium 372) Composition: NaCl 200.0 g/l MgSO4 x 7 H2O 20.0 g/l Agar 20.0 g/l Yeast extract 5.0 g/l Casamino acids 5.0 g/l Na3-citrate 3.0 g/l KCl 2.0 g/l Na glutamate 1.0 g/l FeCl2 x 4 H2O 0.036 g/l MnCl2 x 4 H2O 0.00036 g/l Distilled water |
33788 | MEDIUM 204 - for Halorubrum, Marinococcus and Natrialba | yes | Distilled water make up to (1000.000 ml);Sodium chloride (200.000 g);Potassium chloride (2.000 g);ManganeseII chloride tetrahydrate (0.360 mg);Magnesium sulphate heptahydrate (20.000 g);Agar (20.000 g);Yeast extract (5.000 g);Ferrous chloride tetrahydrate | |
120661 | CIP Medium 204 | yes | https://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=204 |
culture temp
@ref | growth | type | temperature | range |
---|---|---|---|---|
5606 | positive | growth | 37 | mesophilic |
33788 | positive | growth | 37 | mesophilic |
67770 | positive | growth | 37 | mesophilic |
120661 | positive | growth | 37-41 | |
120661 | no | growth | 5 | psychrophilic |
120661 | no | growth | 15 | psychrophilic |
120661 | no | growth | 30 | mesophilic |
120661 | no | growth | 45 | thermophilic |
Physiology and metabolism
halophily
@ref | salt | growth | tested relation | concentration |
---|---|---|---|---|
120661 | NaCl | no | growth | 0 % |
120661 | NaCl | no | growth | 2 % |
120661 | NaCl | no | growth | 4 % |
120661 | NaCl | no | growth | 6 % |
120661 | NaCl | no | growth | 8 % |
120661 | NaCl | no | growth | 10 % |
metabolite utilization
@ref | Chebi-ID | metabolite | utilization activity | kind of utilization tested |
---|---|---|---|---|
120661 | 16947 | citrate | - | carbon source |
120661 | 4853 | esculin | - | hydrolysis |
120661 | 606565 | hippurate | - | hydrolysis |
120661 | 17632 | nitrate | - | reduction |
120661 | 16301 | nitrite | - | reduction |
120661 | 15792 | malonate | - | assimilation |
metabolite production
- @ref: 120661
- Chebi-ID: 35581
- metabolite: indole
- production: no
metabolite tests
- @ref: 120661
- Chebi-ID: 15688
- metabolite: acetoin
- voges-proskauer-test: +
enzymes
@ref | value | activity | ec |
---|---|---|---|
68382 | alpha-fucosidase | - | 3.2.1.51 |
68382 | alpha-mannosidase | - | 3.2.1.24 |
68382 | N-acetyl-beta-glucosaminidase | - | 3.2.1.52 |
68382 | beta-glucosidase | - | 3.2.1.21 |
68382 | alpha-glucosidase | - | 3.2.1.20 |
68382 | beta-glucuronidase | - | 3.2.1.31 |
68382 | beta-galactosidase | - | 3.2.1.23 |
68382 | alpha-galactosidase | - | 3.2.1.22 |
68382 | naphthol-AS-BI-phosphohydrolase | - | |
68382 | acid phosphatase | - | 3.1.3.2 |
68382 | alpha-chymotrypsin | - | 3.4.21.1 |
68382 | trypsin | - | 3.4.21.4 |
68382 | cystine arylamidase | - | 3.4.11.3 |
68382 | valine arylamidase | - | |
68382 | leucine arylamidase | + | 3.4.11.1 |
68382 | lipase (C 14) | - | |
68382 | esterase lipase (C 8) | + | |
68382 | esterase (C 4) | + | |
68382 | alkaline phosphatase | - | 3.1.3.1 |
120661 | oxidase | - | |
120661 | beta-galactosidase | - | 3.2.1.23 |
120661 | alcohol dehydrogenase | - | 1.1.1.1 |
120661 | gelatinase | - | |
120661 | catalase | + | 1.11.1.6 |
120661 | gamma-glutamyltransferase | - | 2.3.2.2 |
120661 | lysine decarboxylase | - | 4.1.1.18 |
120661 | ornithine decarboxylase | - | 4.1.1.17 |
120661 | phenylalanine ammonia-lyase | - | 4.3.1.24 |
120661 | tryptophan deaminase | - | |
120661 | urease | - | 3.5.1.5 |
API zym
@ref | Control | Alkaline phosphatase | Esterase | Esterase Lipase | Lipase | Leucine arylamidase | Valine arylamidase | Cystine arylamidase | Trypsin | alpha- Chymotrypsin | Acid phosphatase | Naphthol-AS-BI-phosphohydrolase | alpha- Galactosidase | beta- Galactosidase | beta- Glucuronidase | alpha- Glucosidase | beta- Glucosidase | N-acetyl-beta- glucosaminidase | alpha- Mannosidase | alpha- Fucosidase |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
120661 | - | - | + | + | - | + | - | - | - | - | - | - | - | - | - | - | - | - | - | - |
Isolation, sampling and environmental information
isolation
@ref | sample type | geographic location | country | origin.country | continent | isolation date |
---|---|---|---|---|---|---|
5606 | water of a saltern | Alicante | Spain | ESP | Europe | |
67770 | Saltern in Alicante | Spain | ESP | Europe | ||
120661 | Environment, Water of a saltern | Alicante | Spain | ESP | Europe | 1986 |
isolation source categories
Cat1 | Cat2 |
---|---|
#Environmental | #Aquatic |
#Condition | #Saline |
taxonmaps
- @ref: 69479
- File name: preview.99_187105.png
- url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_17828;96_68792;97_89434;98_121704;99_187105&stattab=map
- Last taxonomy: Haloferax
- 16S sequence: AB663378
- Sequence Identity:
- Total samples: 8
- soil counts: 1
- aquatic counts: 7
Safety information
risk assessment
@ref | biosafety level | biosafety level comment |
---|---|---|
5606 | 1 | Risk group (German classification) |
120661 | 1 | Risk group (French classification) |
Sequence information
16S sequences
@ref | description | accession | length | database | NCBI tax ID |
---|---|---|---|---|---|
20218 | Haloferax lucentense DSM 14919 strain Aa 2.2 16S ribosomal RNA gene, partial sequence | M33803 | 394 | ena | 1230452 |
20218 | Haloferax lucentense DSM 14919 strain Aa 2.2 16S ribosomal RNA gene, partial sequence | M33804 | 254 | ena | 1230452 |
20218 | Haloferax lucentense DSM 14919 strain Aa 2.2 16S ribosomal RNA gene, partial sequence | M33805 | 539 | ena | 1230452 |
20218 | Haloferax lucentensis gene for 16S rRNA | AB081732 | 1473 | ena | 2254 |
5606 | Haloferax lucentense gene for 16S rRNA, complete sequence, strain: JCM 9276 | AB663378 | 1473 | ena | 2254 |
67770 | Haloferax lucentense DSM 14919 strain Aa 2.2 16S ribosomal RNA genes, partial sequence | AH003665 | 1387 | ena | 1230452 |
Genome sequences
@ref | description | accession | assembly level | database | NCBI tax ID |
---|---|---|---|---|---|
66792 | Haloferax lucentense DSM 14919 | 2554235494 | draft | img | 1230452 |
67770 | Haloferax lucentense DSM 14919 | GCA_000336795 | contig | ncbi | 1230452 |
66792 | Haloferax lucentense DSM 14919 | 1230452.3 | wgs | patric | 1230452 |
GC content
- @ref: 67770
- GC-content: 64.5
- method: thermal denaturation, midpoint method (Tm)
External links
@ref: 5606
culture collection no.: DSM 14919, CCM 7023, CECT 5871, CIP 107410, JCM 9276, CGMCC 1.2151, CGMCC 1.5396, CGMCC 1.6161, NCIMB 13854
straininfo link
- @ref: 75418
- straininfo: 65751
literature
- topic: Phylogeny
- Pubmed-ID: 12486456
- title: Taxonomic characterization of Haloferax sp. (" H. alicantei") strain Aa 2.2: description of Haloferax lucentensis sp. nov.
- authors: Gutierrez MC, Kamekura M, Holmes ML, Dyall-Smith ML, Ventosa A
- journal: Extremophiles
- DOI: 10.1007/s00792-002-0282-7
- year: 2002
- mesh: Bacterial Typing Techniques, Chromatography, Thin Layer, DNA, Bacterial/genetics, DNA, Ribosomal/genetics, Haloarcula/classification, Haloferax/*classification/genetics/growth & development/metabolism, Membrane Lipids/analysis, Nucleic Acid Hybridization, Phylogeny, RNA, Bacterial/genetics, RNA, Ribosomal, 16S/genetics, Ribotyping, Spain
- topic2: Metabolism
Reference
@id | authors | catalogue | doi/url | title | journal | pubmed |
---|---|---|---|---|---|---|
5606 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 14919) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-14919 | |||
20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |||
20218 | Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P. | 10.1016/j.syapm.2013.11.002 | StrainInfo introduces electronic passports for microorganisms. | Syst Appl Microbiol. 37: 42-50 2014 | 24321274 | |
33788 | Curators of the CIP | https://brclims.pasteur.fr/brcWeb/souche/detail/1/4843 | ||||
66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |||
67770 | Curators of the JCM | https://jcm.brc.riken.jp/en/ | ||||
68382 | Automatically annotated from API zym | |||||
69479 | João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich. | https://microbeatlas.org/ | MicrobeAtlas 1.0 beta | |||
75418 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID65751.1 | StrainInfo: A central database for resolving microbial strain identifiers | |||
120661 | Curators of the CIP | Collection of Institut Pasteur (CIP 107410) | https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20107410 |