Haloferax sulfurifontis (DSM 16227, CCM 7217, CIP 108334)

Name: Haloferax sulfurifontis (DSM 16227, CCM 7217, CIP 108334)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 5936

Type strain

Strain Designation M6

Culture col. no. DSM 16227 CCM 7217 CIP 108334 JCM 12327 M6 2 more

NCBI tax ID(s) 662480 255616

Special Collections DSMZ JCM CIP

Links

Haloferax sulfurifontis M6 is a mesophilic, Gram-negative, ovoid-shaped prokaryote that was isolated from microbial mats in a sulfur spring.

Gram-negative ovoid-shaped mesophilic genome sequence 16S sequence

Name and taxonomic classification

SI-ID 134426

ATCC BAA-897,CCM 7217,CIP 108334,DSM 16227,JCM 12327

@ref 20215

Last LPSN update 2025-12-16 09:48:11

Domain Methanobacteriati

Phylum Methanobacteriota

Class Halobacteria

Order Halobacteriales

Family Haloferacaceae

Genus Haloferax

Species Haloferax sulfurifontis

Full scientific name Haloferax sulfurifontis Elshahed et al. 2004

BacDive ID	Other strains from **Haloferax sulfurifontis** (1)	Type strain
175113	H. sulfurifontis 4426B, DSM 116515

Morphology

Cell morphology

@ref	Gram stain	Cell shape	Motility
123386	negative	ovoid-shaped

Culture and growth conditions

Culture medium

@ref	Name	Medium link	Composition
6342	HALOFERAX SULFURIFONTIS MEDIUM (DSMZ Medium 1018)	Medium recipe at MediaDive	Name: HALOFERAX SULFURIFONTIS MEDIUM (DSMZ Medium 1018) Composition: NaCl 150.0 g/l MgCl2 20.0 g/l Agar 20.0 g/l Yeast extract 5.0 g/l K2SO4 0.5 g/l CaCl2 0.1 g/l Distilled water
33946	MEDIUM 204 - for Halorubrum, Marinococcus and Natrialba		Distilled water make up to (1000.000 ml);Sodium chloride (200.000 g);Potassium chloride (2.000 g);ManganeseII chloride tetrahydrate (0.360 mg);Magnesium sulphate heptahydrate (20.000 g);Agar (20.000 g);Yeast extract (5.000 g);Ferrous chloride tetrahydrate
123386	CIP Medium 204	Medium recipe at CIP

Culture temp

@ref	Growth	Type	Temperature (°C)	Range
6342	positive	growth	37	mesophilic
33946	positive	growth	37	mesophilic
67770	positive	growth	37	mesophilic
123386	positive	growth	5-45

Physiology and metabolism

Spore formation

@ref	Spore formation	Confidence
125439		96.7

Halophily

@ref	Salt	Tested relation	Concentration
123386	NaCl	growth	0 %
123386	NaCl	growth	2 %
123386	NaCl	growth	4 %
123386	NaCl	growth	6 %
123386	NaCl	growth	8 %
123386	NaCl	growth	10 %

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
123386	16947 ChEBI	citrate	-	carbon source
123386	4853 ChEBI	esculin	+	hydrolysis
123386	17234 ChEBI	glucose	-	degradation
123386	606565 ChEBI	hippurate	-	hydrolysis
123386	15792 ChEBI	malonate	-	assimilation
123386	17632 ChEBI	nitrate	+	reduction
123386	16301 ChEBI	nitrite	-	reduction

Metabolite production

@ref	Chebi-ID	Metabolite	Production
123386	35581 ChEBI	indole

Metabolite tests

@ref	Chebi-ID	Metabolite	Voges-proskauer-test
123386	15688 ChEBI	acetoin	-
123386	17234 ChEBI	glucose

Enzymes

@ref	Value	Activity	Ec
123386	alcohol dehydrogenase	-	1.1.1.1
123386	beta-galactosidase	-	3.2.1.23
123386	catalase	+	1.11.1.6
123386	gamma-glutamyltransferase	-	2.3.2.2
123386	gelatinase	-
123386	lysine decarboxylase	-	4.1.1.18
123386	ornithine decarboxylase	-	4.1.1.17
123386	oxidase	+
123386	phenylalanine ammonia-lyase	-	4.3.1.24
123386	tryptophan deaminase	-
123386	urease	-	3.5.1.5

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Environmental	#Aquatic	#Spring
#Environmental	#Microbial community	#Microbial mat
#Condition	#Sulfuric	-

Isolation

@ref	Sample type	Geographic location	Country	Country ISO 3 Code	Continent
6342	microbial mats in a sulfur spring	Oklahoma	USA	USA	North America
67770	Microbial mat at Zodletone Spring in southwestern Okulahoma		USA	USA	North America
123386	Environment, Water sample	Oklahoma	United States of America	USA	North America

Taxonmaps

69479

Global distribution of 16S sequence AB477973 (>99% sequence identity) for Haloferax from Microbeatlas

Aquatic Samples	8
Soil Samples	2
Animal Samples
Plant Samples
Total Samples	10

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
6342	1	Risk group (German classification) According to TRBA 466
123386	1	Risk group (French classification) According to TRBA 466

Sequence information

Genome sequences

@ref	Description	Assembly level	IMG accession	NCBI tax ID	Score
66792	Haloferax sulfurifontis M6	complete	2508501015	662480	92.96
67770	ASM33783v1 assembly for Haloferax sulfurifontis ATCC BAA-897	contig	2529293243	662480	71.61
66792	ASM1463510v1 assembly for Haloferax sulfurifontis CCM 7217	contig	8112497867	255616	69.86

16S sequences

@ref	Description	Accession	Length	NCBI tax ID
20218	Haloferax sulfurifontis gene for 16S ribosomal RNA, partial sequence, strain: JCM 12327	AB477973	1434	662480
20218	Haloferax sulfurifontis gene for 16S rRNA, complete sequence, strain: JCM 12327	AB663382	1474	662480
6342	Haloferax sulfurifontis 16S ribosomal RNA gene, partial sequence	AY458601	1409	662480
124043	Haloferax sulfurifontis strain CCM 7217 16S ribosomal RNA gene, partial sequence.	MT758005	790	255616
124043	Haloferax sulfurifontis strain CCM 7217 16S ribosomal RNA gene, partial sequence.	MT760074	790	255616

GC content

@ref	GC-content (mol%)	Method
67770	60.5	thermal denaturation, midpoint method (Tm)

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: Haloferax sulfurifontis CCM 7217
NCBI: GCA_014635105

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	spore_formation	BacteriaNetⓘ	no	96.70	no
125439	motility	BacteriaNetⓘ	yes	65.20	no
125439	gram_stain	BacteriaNetⓘ	negative	85.30	no
125439	oxygen_tolerance	BacteriaNetⓘ	obligate anaerobe	64.50	no

Literature

Title	Authors	Journal	DOI	Year
Microbial communities mediating algal detritus turnover under anaerobic conditions.	Morrison JM, Murphy CL, Baker K, Zamor RM, Nikolai SJ, Wilder S, Elshahed MS, Youssef NH.	PeerJ	10.7717/peerj.2803	2017
Haloferax sulfurifontis GUMFAZ2 producing xylanase-free cellulase retrieved from Haliclona sp. inhabiting rocky shore of Anjuna, Goa-India.	Malik AD, Furtado IJ.	J Basic Microbiol	10.1002/jobm.201800672	2019
Establishment of a Halophilic Bloom in a Sterile and Isolated Hypersaline Mesocosm.	Rhodes ME, Pace AD, Benjamin MM, Ghent H, Dawson KS.	Microorganisms	10.3390/microorganisms11122886	2023
Isolation of Halomicroarcula pellucida strain GUMF5, an archaeon from the Dead Sea-Israel possessing cellulase.	Malik AD, Furtado IJ.	3 Biotech	10.1007/s13205-021-03090-2	2022
Using custom-built primers and nanopore sequencing to evaluate CO-utilizer bacterial and archaeal populations linked to bioH₂ production.	Akacin I, Ersoy S, Doluca O, Gungormusler M.	Sci Rep	10.1038/s41598-023-44357-3	2023
Halophilic Archaea Mediate the Formation of Proto-Dolomite in Solutions With Various Sulfate Concentrations and Salinities.	Qiu X, Yao Y, Wang H, Shen A, Zhang J.	Front Microbiol	10.3389/fmicb.2019.00480	2019
Ubiquitousness of Haloferax and Carotenoid Producing Genes in Arabian Sea Coastal Biosystems of India.	Moopantakath J, Imchen M, Kumavath R, Martinez-Espinosa RM.	Mar Drugs	10.3390/md19080442	2021
Halophilic archaea and their potential to generate renewable fuels and chemicals.	Kasirajan L, Maupin-Furlow JA.	Biotechnol Bioeng	10.1002/bit.27639	2021
Computational Exploration of Putative LuxR Solos in Archaea and Their Functional Implications in Quorum Sensing.	Rajput A, Kumar M.	Front Microbiol	10.3389/fmicb.2017.00798	2017
Rhodanese-Like Domain Protein UbaC and Its Role in Ubiquitin-Like Protein Modification and Sulfur Mobilization in Archaea.	Hepowit NL, Maupin-Furlow JA.	J Bacteriol	10.1128/jb.00254-19	2019
What Is the Role of Archaea in Plants? New Insights from the Vegetation of Alpine Bogs.	Taffner J, Erlacher A, Bragina A, Berg C, Moissl-Eichinger C, Berg G.	mSphere	10.1128/msphere.00122-18	2018
Potential applications of halophilic microorganisms for biological treatment of industrial process brines contaminated with aromatics.	Mainka T, Weirathmuller D, Herwig C, Pflugl S.	J Ind Microbiol Biotechnol	10.1093/jimb/kuab015	2021
Microbial life at high salt concentrations: phylogenetic and metabolic diversity.	Oren A.	Saline Syst	10.1186/1746-1448-4-2	2008
Inteins as indicators of gene flow in the halobacteria.	Soucy SM, Fullmer MS, Papke RT, Gogarten JP.	Front Microbiol	10.3389/fmicb.2014.00299	2014
A traditional Japanese-style salt field is a niche for haloarchaeal strains that can survive in 0.5% salt solution.	Fukushima T, Usami R, Kamekura M.	Saline Syst	10.1186/1746-1448-3-2	2007
Quantifying homologous replacement of loci between haloarchaeal species.	Williams D, Gogarten JP, Papke RT.	Genome Biol Evol	10.1093/gbe/evs098	2012
Haloferax sulfurifontis sp. nov., a halophilic archaeon isolated from a sulfide- and sulfur-rich spring.	Elshahed MS, Savage KN, Oren A, Gutierrez MC, Ventosa A, Krumholz LR	Int J Syst Evol Microbiol	10.1099/ijs.0.63211-0	2004

References

#6342	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 16227
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#20218	Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )
#33946	; Curators of the CIP;
#66792	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .
#67770	Japan Collection of Microorganism (JCM) ; Curators of the JCM;
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#123386	Collection of Institut Pasteur ; Curators of the CIP; CIP 108334
#124043	Isabel Schober, Julia Koblitz: Data extracted from sequence databases, automatically matched based on designation and taxonomy .
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Haloferax sulfurifontis (DSM 16227, CCM 7217, CIP 108334)

Name and taxonomic classification

Morphology

Cell morphology

Culture and growth conditions

Culture medium

Culture temp

Physiology and metabolism

Spore formation

Halophily

Metabolite utilization

Metabolite production

Metabolite tests

Enzymes

Isolation, sampling and environmental information

Isolation source categories

Isolation

Taxonmaps

Interaction and safety

Risk assessment

Sequence information

Genome sequences

16S sequences

GC content

Genome-based predictions

Predictions

Literature

Literature

References

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