Name: Fusobacterium necrophorum Fievez 2358
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 5760

Type strain:

Species: Fusobacterium necrophorum

Strain Designation: Fievez 2358, Moore VPI 2891, 2358, 10145

Culture col. no.: CCUG 4948, DSM 21784, ATCC 25286, CCUG 9994, CIP 104559, DSM 19788, JCM 3718, VPI 2891, BCRC 10679, NCTC 13726

Strain history: CIP <- 1995, J.P. Carlier, Inst. Pasteur, Paris, France: strain 10145 <- ATCC <- W.E.C. Moore: strain VPI 2891 <- L. Fievez: strain 2358

NCBI tax ID(s): 859 (species)

SI-ID 38464

ATCC 25286, JCM 3718, CCUG 9994, CIP 104559, CCRC 10679, CCUG 4948, BCRC 10679, DSM 19788, VTT E-001739

Other strains from Fusobacterium necrophorum

F. necrophorum CCUG 6476 B
F. necrophorum CCUG 6954
F. necrophorum CCUG 21045 A
F. necrophorum CCUG 26835
F. necrophorum CCUG 34059
F. necrophorum CCUG 37310
F. necrophorum CCUG 38803
F. necrophorum CCUG 39257
F. necrophorum CCUG 44396
F. necrophorum CCUG 49004
F. necrophorum CCUG 53692
F. necrophorum CCUG 53693
F. necrophorum CCUG 53804
F. necrophorum CCUG 56583
F. necrophorum CCUG 59120
F. necrophorum CCUG 62210

Section

Fusobacterium necrophorum Fievez 2358 is an anaerobe, mesophilic, Gram-negative human pathogen that was isolated from bovine hepatic abscess.

Gram-negative
motile
rod-shaped
anaerobe
human pathogen
mesophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Fusobacteriota*
	Class	*Fusobacteriia*
	Order	*Fusobacteriales*
	Family	*Fusobacteriaceae*
	Genus	*Fusobacterium*
	Species	**Fusobacterium necrophorum**
	Full Scientific Name (LPSN)	Fusobacterium necrophorum (Flügge 1886) Moore and Holdeman 1969 (Approved Lists 1980)

Synonym

"Bacillus necrophorus"

Information on morphological and physiological properties Morphology

[Ref.: #121252]	Gram stain	negative

[Ref.: #121252]	Cell shape	rod-shaped

[Ref.: #121252]	Motility	yes

[Ref.: #15948]	Incubation period	2-3 days

[Ref.: #45248]	Incubation period	3 days

Information on culture and growth conditions Culture and growth conditions

[Ref.: #15948]

Culture medium

COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)

[Ref.: #15948]

Culture medium growth

[Ref.: #15948]

Culture medium link

Medium recipe at MediaDive

[Ref.: #15948]

Culture medium composition

expand / minimize

[Ref.: #15948]

Culture medium

PYG MEDIUM (MODIFIED) (DSMZ Medium 104)

[Ref.: #15948]

Culture medium growth

[Ref.: #15948]

Culture medium link

Medium recipe at MediaDive

[Ref.: #15948]

Culture medium composition

expand / minimize

[Ref.: #41710]

Culture medium

MEDIUM 20 - for Anaerobic bacteria

[Ref.: #41710]

Culture medium growth

[Ref.: #41710]

Culture medium composition

expand / minimize

[Ref.: #121252]

Culture medium

CIP Medium 20

[Ref.: #121252]

Culture medium growth

[Ref.: #121252]

Culture medium link

Medium recipe provided by DSMZ

		Temperature
[Ref.: #15948]	growth	37 °C
[Ref.: #41710]	growth	37 °C
[Ref.: #45248]	growth	37 °C
[Ref.: #67770]	growth	37 °C

[Ref.: #15948]	Temperature range	mesophilic
[Ref.: #41710]	Temperature range	mesophilic
[Ref.: #45248]	Temperature range	mesophilic
[Ref.: #67770]	Temperature range	mesophilic

Information on physiology and metabolism Physiology and metabolism

[Ref.: #15948]	Oxygen tolerance	anaerobe
[Ref.: #45248]	Oxygen tolerance	anaerobe

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #68380]	arginine ChEBI	-	hydrolysis	* from API rID32A
[Ref.: #68380]	D-mannose ChEBI	-	fermentation	* from API rID32A
[Ref.: #68380]	L-glutamate ChEBI	-	degradation	* from API rID32A
[Ref.: #68380]	nitrate ChEBI	-	reduction	* from API rID32A
[Ref.: #68380]	raffinose ChEBI	-	fermentation	* from API rID32A
[Ref.: #68380]	tryptophan ChEBI	+	energy source	* from API rID32A
[Ref.: #68380]	urea ChEBI	-	hydrolysis	* from API rID32A

	Metabolite production	Metabolite	Production
[Ref.: #68380]		indole ChEBI	yes	* from API rID32A

	Physiological tests	Metabolite	Indole test
[Ref.: #68380]		indole ChEBI	+	* from API rID32A

	Enzyme	Enzyme activity	EC number
[Ref.: #68380]	alanine arylamidase	-	3.4.11.2	* from API rID32A
[Ref.: #68380]	alkaline phosphatase	+	3.1.3.1	* from API rID32A
[Ref.: #68380]	alpha-arabinosidase	-	3.2.1.55	* from API rID32A
[Ref.: #68380]	alpha-fucosidase	-	3.2.1.51	* from API rID32A
[Ref.: #68380]	alpha-galactosidase	-	3.2.1.22	* from API rID32A
[Ref.: #68380]	alpha-glucosidase	-	3.2.1.20	* from API rID32A
[Ref.: #68380]	arginine dihydrolase	-	3.5.3.6	* from API rID32A
[Ref.: #68380]	beta-galactosidase	-	3.2.1.23	* from API rID32A
[Ref.: #68380]	beta-Galactosidase 6-phosphate	-		* from API rID32A
[Ref.: #68380]	beta-glucosidase	-	3.2.1.21	* from API rID32A
[Ref.: #68380]	beta-glucuronidase	-	3.2.1.31	* from API rID32A
[Ref.: #68380]	glutamate decarboxylase	-	4.1.1.15	* from API rID32A
[Ref.: #68380]	glutamyl-glutamate arylamidase	-		* from API rID32A
[Ref.: #68380]	glycin arylamidase	-		* from API rID32A
[Ref.: #68380]	histidine arylamidase	-		* from API rID32A
[Ref.: #68380]	L-arginine arylamidase	-		* from API rID32A
[Ref.: #68380]	leucine arylamidase	-	3.4.11.1	* from API rID32A
[Ref.: #68380]	leucyl glycin arylamidase	-	3.4.11.1	* from API rID32A
[Ref.: #68380]	N-acetyl-beta-glucosaminidase	-	3.2.1.52	* from API rID32A
[Ref.: #68380]	phenylalanine arylamidase	-		* from API rID32A
[Ref.: #68380]	proline-arylamidase	-	3.4.11.5	* from API rID32A
[Ref.: #68380]	pyrrolidonyl arylamidase	-	3.4.19.3	* from API rID32A
[Ref.: #68380]	serine arylamidase	-		* from API rID32A
[Ref.: #68380]	tryptophan deaminase	+	4.1.99.1	* from API rID32A
[Ref.: #68380]	tyrosine arylamidase	-		* from API rID32A
[Ref.: #68380]	urease	-	3.5.1.5	* from API rID32A
	Only first 10 entries are displayed. Click here to see all.

Fatty acid profile

Metadata FA analysis

Reference

[Ref.: #45248]

Fatty acid	Percentage	ECL
C16:0	30	16
C18:2 ω6,9c/C18:0 ANTE	17.5	17.724
C14:0	17	14
C18:1 ω9c	7.7	17.769
C14:0 3OH/C16:1 ISO I	6.9	15.485
C16:1 ω7c	5.4	15.819
C18:0	3.9	18
C12:0	3	12
C17:1 ISO I/C16:0 DMA	2.3	16.481
C16:1 ω9c	1.8	15.774
C18:1 ω7c /12t/9t	1.8	17.824
C15:0	0.7	15
C13:0 3OH/C15:1 i I/H	0.6	14.469
C12:0 ALDE ?	0.4	10.915
C17:0	0.4	17
C16:0 aldehyde	0.3	14.949
C13:0 ISO 2OH	0.3	13.814

API® rID32A:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Urease/urea hydrolysisURE	Arginine dihydrolaseADH (Arg)	alpha-Galactosidasealpha GAL	beta-Galactosidasebeta GAL	beta-Galactosidase 6-phosphatebeta GP	alpha-Glucosidasealpha GLU	beta-Glucosidasebeta GLU	alpha-Arabinosidasealpha ARA	beta-Glucuronidasebeta GUR	beta-N-Acetyl-beta-glucosaminidasebeta NAG	Fermentation of D-mannoseMNE	Fermentation of raffinoseRAF	Glutamate decarboxylaseGDC	alpha-Fucosidasealpha FUC	Reduction of nitrateNIT	Indole productionIND	Alkaline phosphatasePAL	L-arginine arylamidaseArgA	Proline arylamidaseProA	Leucyl glycin arylamidaseLGA	Phenylalanine arylamidasePheA	Leucine arylamidaseLeuA	Pyrrolidonyl arylamidasePyrA	Tyrosine arylamidaseTyrA	Alanine arylamidaseAlaA	Glycin arylamidaseGlyA	Histidine arylamidaseHisA	Glutamyl-glutamate arylamidaseGGA	Serine arylamidaseSerA
[Ref.: #15948]	13306	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	+	+	-	-	-	-	-	-	-	-	-	-	-	-
[Ref.: #15948]	13004	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	+	+	-	-	-	-	-	-	-	-	-	-	-	-
[Ref.: #15948]	12394	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	+	+	-	-	-	-	-	-	-	-	-	-	-	-
[Ref.: #15948]	12393	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	+	+	-	-	-	-	-	-	-	-	-	-	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #15948]	Sample type/isolated from	bovine hepatic abscess

[Ref.: #67770]	Sample type/isolated from	Bovine liver abscess
* marker position based on {}

Isolation sources categories

#Host	#Mammals	#Bovinae (Cow, Cattle)
#Host Body-Site	#Other	#Abscess
#Host Body-Site	#Organ	#Liver

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence AB849121 (>99% sequence identity) for Fusobacterium necrophorum from Microbeatlas

Aquatic Samples	1051
Soil Samples	1769
Animal Samples	26978
Plant Samples	271
Total Samples	30069

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #15948]	Pathogenicity (human)	yes
[Ref.: #15948]	Pathogenicity (animal)	yes
[Ref.: #15948]	Biosafety level	2	Risk group (German classification) According to TRBA 466
[Ref.: #121252]	Biosafety level	2	Risk group (French classification)

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

Only first 5 entries are displayed. Click here to see all.

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #15948]	Fusobacterium necrophorum 16S rRNA gene, strain ATCC 25286	AJ867039	1455	ENA	859 tax ID
[Ref.: #20218]	Fusobacterium necrophorum subsp. necrophorum strain ATCC 25286 16S ribosomal RNA gene, partial sequence; internal transcribed spacer 1, complete sequence; and 23S ribosomal RNA gene, partial sequence	AF342842	746	ENA	143388 tax ID	*
[Ref.: #20218]	Fusobacterium necrophorum subsp. necrophorum gene for 16S ribosomal RNA, partial sequence, strain: JCM 3718	AB849121	1469	ENA	143388 tax ID	*
[Ref.: #20218]	Fusobacterium necrophorum rRNA small subunit	X55411	1333	ENA	859 tax ID	*
[Ref.: #20218]	Fusobacterium necrophorum subsp. necrophorum strain JCM 3718 clone 1 16S ribosomal RNA gene, partial sequence; 16S-23S intergenic spacer, tRNA-Ile, and tRNA-Ala genes, complete sequence; and 23S ribosomal RNA gene, partial sequence	AF369872	527	ENA	143388 tax ID	*
[Ref.: #20218]	Fusobacterium necrophorum subsp. necrophorum strain JCM 3718 clone 2 16S ribosomal RNA gene, intergenic spacer, and 23S ribosomal RNA gene, partial sequence	AF369873	359	ENA	143388 tax ID	*
[Ref.: #20218]	Fusobacterium necrophorum subsp. necrophorum strain JCM 3718 clone 2 16S ribosomal RNA gene, intergenic spacer, and 23S ribosomal RNA gene, partial sequence	AF369875	359	ENA	143388 tax ID	*
[Ref.: #20218]	Fusobacterium necrophorum ATCC:25286 16S-23S ribosomal intergenic spacer and 23S ribosomal RNA gene, partial sequence	FJ410389	3086	ENA	859 tax ID	*
[Ref.: #20218]	Fusobacterium necrophorum subsp. necrophorum strain JCM3718 16S ribosomal RNA and 23S ribosomal RNA genes, partial sequence	AF410969	358	ENA	143388 tax ID	*

Genome sequence information:

Only first 5 entries are displayed. Click here to see all.

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #67770]	Fusobacterium necrophorum ATCC 25286	GCA_900104395	scaffold	GenBank	859 tax ID
[Ref.: #67770]	Fusobacterium necrophorum subsp. necrophorum NCTC13726	GCA_900451075	contig	GenBank	143388 tax ID
[Ref.: #66792]	Fusobacterium necrophorum subsp. necrophorum strain ATCC 25286	143388.7	complete	PATRIC	143388 tax ID	*
[Ref.: #66792]	Fusobacterium necrophorum subsp. necrophorum strain NCTC13726	143388.5	wgs	PATRIC	143388 tax ID	*
[Ref.: #66792]	Fusobacterium necrophorum ATCC 25286	2847466697	complete	JGI IMG/M	859 tax ID	*
[Ref.: #66792]	Fusobacterium necrophorum necrophorum NCTC 13726	2859123070	draft	JGI IMG/M	143388 tax ID	*

[Ref.: #15948]	GC-content	28 mol%	thermal denaturation, midpoint method (Tm)
[Ref.: #67770]	GC-content	32.3 mol%	thermal denaturation, midpoint method (Tm)

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Fusobacterium necrophorum subsp. necrophorum ATCC 25286 NCBI: GCA_004006635.1
[Ref.: #69480]	motile	no	92.174	no
[Ref.: #69480]	flagellated	no	94.973	no
[Ref.: #69480]	gram-positive	no	90.284	no
[Ref.: #69480]	anaerobic	yes	99.743	yes
[Ref.: #69480]	aerobic	no	98.365	yes
[Ref.: #69480]	halophile	no	81.166	no
[Ref.: #69480]	spore-forming	no	88.998	no
[Ref.: #69480]	thermophile	no	97.676	no
[Ref.: #69480]	glucose-util	yes	83.279	no
[Ref.: #69480]	glucose-ferment	yes	57.071	no

Availability in culture collections External links

[Ref.: #15948]

Culture collection no.

CCUG 4948, DSM 21784, ATCC 25286, CCUG 9994, CIP 104559, DSM 19788, JCM 3718, VPI 2891, BCRC 10679, NCTC 13726

[Ref.: #75239]

SI-ID 38464

Literature:

Only first 10 entries are displayed. Click here to see all.

Topic	Title	Authors	Journal	DOI	Year
Pathogenicity	Chemical composition and antibacterial activity of essential oils against pathogens often related to cattle endometritis.	Braga Paiano R, Bonilla J, Moro de Sousa RL, Micke Moreno A, Sampaio Baruselli P	J Infect Dev Ctries	10.3855/jidc.12076	2020	*
Genetics	Complete Genome Sequence of Fusobacterium necrophorum subsp. necrophorum ATCC 25286.	Umana A, Lemkul JA, Slade DJ	Microbiol Resour Announc	10.1128/MRA.00025-19	2019	*
Metabolism	[Design of artificial foetor flatus based on bacterial volatile compounds].	Justesen US	Ugeskr Laeger	V68707	2016	*
Metabolism	Stimulation of epithelial cell matrix metalloproteinase (MMP-2, -9, -13) and interleukin-8 secretion by fusobacteria.	Gursoy UK, Kononen E, Uitto VJ	Oral Microbiol Immunol	10.1111/j.1399-302X.2008.00453.x	2008	*
Enzymology	Fusobacterium necrophorum as the cause of recurrent sore throat: comparison of isolates from persistent sore throat syndrome and Lemierre's disease.	Batty A, Wren MW, Gal M	J Infect	10.1016/j.jinf.2004.09.013	2004	*
Metabolism	Adherence of Fusobacterium necrophorum subsp. necrophorum to ruminal cells derived from bovine rumenitis.	Takayama Y, Kanoe M, Maeda K, Okada Y, Kai K	Lett Appl Microbiol	10.1046/j.1472-765x.2000.00722.x	2000	*
Enzymology	Location of haemagglutinin in bacterial cells of Fusobacterium necrophorum subsp. necrophorum.	Kanoe M, Koyanagi Y, Kondo C, Mamba K, Makita T, Kai K	Microbios		1998	*
Metabolism	Local skin reaction in mice and guinea pigs induced by a single intradermal inoculation of Fusobacterium necrophorum lipopolysaccharide.	Kanoe M, Kiritani M, Inoue M	Microbios		1995	*
Phylogeny	Proposal of two subspecies of Fusobacterium necrophorum (Flugge) Moore and Holdeman: Fusobacterium necrophorum subsp. necrophorum subsp. nov., nom. rev. (ex Flugge 1886), and Fusobacterium necrophorum subsp. funduliforme subsp. nov., nom. rev. (ex Halle 1898).	Shinjo T, Fujisawa T, Mitsuoka T	Int J Syst Bacteriol	10.1099/00207713-41-3-395	1991	*
Phylogeny	Recognition of biovar C of Fusobacterium necrophorum (Flugge) Moore and Holdeman as Fusobacterium pseudonecrophorum sp. nov., nom. rev. (ex Prevot 1940).	Shinjo T, Hiraiwa K, Miyazato S	Int J Syst Bacteriol	10.1099/00207713-40-1-71	1990	*
Enzymology	Structural analysis of lipopolysaccharides from Eikenella corrodens by use of murine monoclonal antibodies.	Kato T, Takazoe I, Okuda K	Infect Immun	10.1128/iai.57.2.656-659.1989	1989	*

References

#15948

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 21784

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#20218

Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )

#41710 ; Curators of the CIP;
#45248 Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 9994

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#68380

Automatically annotated from API rID32A .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#75239

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID38464.1 )

#121252 Collection of Institut Pasteur ; Curators of the CIP; CIP 104559
* These data were automatically processed and therefore are not curated

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Fusobacterium necrophorum subsp. necrophorum ATCC 25286

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