Strain identifier

BacDive ID: 5760

Type strain: Yes

Species: Fusobacterium necrophorum

Strain Designation: Fievez 2358, Moore VPI 2891, 2358, 10145

Strain history: CIP <- 1995, J.P. Carlier, Inst. Pasteur, Paris, France: strain 10145 <- ATCC <- W.E.C. Moore: strain VPI 2891 <- L. Fievez: strain 2358

NCBI tax ID(s): 859 (species)

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General

@ref: 15948

BacDive-ID: 5760

DSM-Number: 21784

keywords: genome sequence, 16S sequence, Bacteria, anaerobe, mesophilic, Gram-negative, motile, rod-shaped, human pathogen

description: Fusobacterium necrophorum Fievez 2358 is an anaerobe, mesophilic, Gram-negative human pathogen that was isolated from bovine hepatic abscess.

NCBI tax id

  • NCBI tax id: 859
  • Matching level: species

strain history

@refhistory
15948<- CCUG; CCUG 9994 <- ATCC; ATCC 25286 <- W. E. C. Moore; Moore VPI 2891 <- L. Fievez; Fievez 2358
67770T. Shinjo Fn 511 (Biovar A) <-- W. E. C. Moore VPI 2891 <-- L. Fievez 2358.
121252CIP <- 1995, J.P. Carlier, Inst. Pasteur, Paris, France: strain 10145 <- ATCC <- W.E.C. Moore: strain VPI 2891 <- L. Fievez: strain 2358

doi: 10.13145/bacdive5760.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/fusobacteriota
  • domain: Bacteria
  • phylum: Fusobacteriota
  • class: Fusobacteriia
  • order: Fusobacteriales
  • family: Fusobacteriaceae
  • genus: Fusobacterium
  • species: Fusobacterium necrophorum
  • full scientific name: Fusobacterium necrophorum (Flügge 1886) Moore and Holdeman 1969 (Approved Lists 1980)

  • synonyms

    • @ref: 20215
    • synonym: Bacillus necrophorus

@ref: 15948

domain: Bacteria

phylum: Fusobacteria

class: Fusobacteriia

order: Fusobacteriales

family: Fusobacteriaceae

genus: Fusobacterium

species: Fusobacterium necrophorum subsp. necrophorum

full scientific name: Fusobacterium necrophorum subsp. necrophorum (Flügge 1886) Shinjo et al. 1991

strain designation: Fievez 2358, Moore VPI 2891, 2358, 10145

type strain: yes

Morphology

cell morphology

  • @ref: 121252
  • gram stain: negative
  • cell shape: rod-shaped
  • motility: yes

colony morphology

@refincubation period
159482-3 days
452483 days

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
15948COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)yeshttps://mediadive.dsmz.de/medium/693Name: COLUMBIA BLOOD MEDIUM (DSMZ Medium 693) Composition: Defibrinated sheep blood 50.0 g/l Columbia agar base
15948PYG MEDIUM (MODIFIED) (DSMZ Medium 104)yeshttps://mediadive.dsmz.de/medium/104Name: PYG MEDIUM (modified) (DSMZ Medium 104) Composition: Yeast extract 10.0 g/l Peptone 5.0 g/l Trypticase peptone 5.0 g/l Beef extract 5.0 g/l Glucose 5.0 g/l L-Cysteine HCl x H2O 0.5 g/l NaHCO3 0.4 g/l NaCl 0.08 g/l K2HPO4 0.04 g/l KH2PO4 0.04 g/l MgSO4 x 7 H2O 0.02 g/l CaCl2 x 2 H2O 0.01 g/l Hemin 0.005 g/l Ethanol 0.0038 g/l Resazurin 0.001 g/l Tween 80 Vitamin K1 NaOH Distilled water
41710MEDIUM 20 - for Anaerobic bacteriayesAgar (15.000 g);Glucose (5.000 g);Yeast extract (20.000 g);Tryptone (30.000 g);Cysteine hydrochloride (0.500 g);distilled water (1000.000 ml);Hemin solution -M00149 (25.000 ml)
121252CIP Medium 20yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=20

culture temp

@refgrowthtypetemperaturerange
15948positivegrowth37mesophilic
41710positivegrowth37mesophilic
45248positivegrowth37mesophilic
67770positivegrowth37mesophilic

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
15948anaerobe
45248anaerobe

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6838016199urea-hydrolysis
6838029016arginine-hydrolysis
6838016024D-mannose-fermentation
6838016634raffinose-fermentation
6838029985L-glutamate-degradation
6838017632nitrate-reduction
6838027897tryptophan+energy source

metabolite production

  • @ref: 68380
  • Chebi-ID: 35581
  • metabolite: indole
  • production: yes

metabolite tests

  • @ref: 68380
  • Chebi-ID: 35581
  • metabolite: indole
  • indole test: +

enzymes

@refvalueactivityec
68380serine arylamidase-
68380glutamyl-glutamate arylamidase-
68380histidine arylamidase-
68380glycin arylamidase-
68380alanine arylamidase-3.4.11.2
68380tyrosine arylamidase-
68380pyrrolidonyl arylamidase-3.4.19.3
68380leucine arylamidase-3.4.11.1
68380phenylalanine arylamidase-
68380leucyl glycin arylamidase-3.4.11.1
68380proline-arylamidase-3.4.11.5
68380L-arginine arylamidase-
68380alkaline phosphatase+3.1.3.1
68380tryptophan deaminase+4.1.99.1
68380alpha-fucosidase-3.2.1.51
68380glutamate decarboxylase-4.1.1.15
68380N-acetyl-beta-glucosaminidase-3.2.1.52
68380beta-glucuronidase-3.2.1.31
68380alpha-arabinosidase-3.2.1.55
68380beta-glucosidase-3.2.1.21
68380alpha-glucosidase-3.2.1.20
68380beta-Galactosidase 6-phosphate-
68380beta-galactosidase-3.2.1.23
68380alpha-galactosidase-3.2.1.22
68380arginine dihydrolase-3.5.3.6
68380urease-3.5.1.5

fatty acid profile

  • fatty acids

    @reffatty acidpercentageECL
    45248C12:0312
    45248C14:01714
    45248C15:00.715
    45248C16:03016
    45248C17:00.417
    45248C18:03.918
    45248C12:0 ALDE ?0.410.915
    45248C13:0 3OH/C15:1 i I/H0.614.469
    45248C13:0 ISO 2OH0.313.814
    45248C14:0 3OH/C16:1 ISO I6.915.485
    45248C16:0 aldehyde0.314.949
    45248C16:1 ω7c5.415.819
    45248C16:1 ω9c1.815.774
    45248C17:1 ISO I/C16:0 DMA2.316.481
    45248C18:1 ω7c /12t/9t1.817.824
    45248C18:1 ω9c7.717.769
    45248C18:2 ω6,9c/C18:0 ANTE17.517.724
  • type of FA analysis: whole cell analysis
  • method/protocol: CCUG

API rID32A

@refUREADH Argalpha GALbeta GALbeta GPalpha GLUbeta GLUalpha ARAbeta GURbeta NAGMNERAFGDCalpha FUCNITINDPALArgAProALGAPheALeuAPyrATyrAAlaAGlyAHisAGGASerA
15948---------------++------------
15948---------------++------------
15948---------------++------------
15948---------------++------------

Isolation, sampling and environmental information

isolation

@refsample type
15948bovine hepatic abscess
67770Bovine liver abscess

isolation source categories

Cat1Cat2Cat3
#Host#Mammals#Bovinae (Cow, Cattle)
#Host Body-Site#Other#Abscess
#Host Body-Site#Organ#Liver

taxonmaps

  • @ref: 69479
  • File name: preview.99_1526.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_140;96_874;97_1022;98_1205;99_1526&stattab=map
  • Last taxonomy: Fusobacterium necrophorum
  • 16S sequence: AB849121
  • Sequence Identity:
  • Total samples: 30069
  • soil counts: 1769
  • aquatic counts: 1051
  • animal counts: 26978
  • plant counts: 271

Safety information

risk assessment

@refpathogenicity humanpathogenicity animalbiosafety levelbiosafety level comment
15948yesyes2Risk group (German classification)
1212522Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Fusobacterium necrophorum subsp. necrophorum gene for 16S ribosomal RNA, partial sequence, strain: JCM 3718AB8491211469ena143388
20218Fusobacterium necrophorum subsp. necrophorum strain ATCC 25286 16S ribosomal RNA gene, partial sequence; internal transcribed spacer 1, complete sequence; and 23S ribosomal RNA gene, partial sequenceAF342842746ena143388
20218Fusobacterium necrophorum subsp. necrophorum strain JCM 3718 clone 1 16S ribosomal RNA gene, partial sequence; 16S-23S intergenic spacer, tRNA-Ile, and tRNA-Ala genes, complete sequence; and 23S ribosomal RNA gene, partial sequenceAF369872527ena143388
20218Fusobacterium necrophorum subsp. necrophorum strain JCM 3718 clone 2 16S ribosomal RNA gene, intergenic spacer, and 23S ribosomal RNA gene, partial sequenceAF369873359ena143388
20218Fusobacterium necrophorum subsp. necrophorum strain JCM 3718 clone 2 16S ribosomal RNA gene, intergenic spacer, and 23S ribosomal RNA gene, partial sequenceAF369875359ena143388
20218Fusobacterium necrophorum subsp. necrophorum strain JCM3718 16S ribosomal RNA and 23S ribosomal RNA genes, partial sequenceAF410969358ena143388
20218Fusobacterium necrophorum ATCC:25286 16S-23S ribosomal intergenic spacer and 23S ribosomal RNA gene, partial sequenceFJ4103893086ena859
20218Fusobacterium necrophorum rRNA small subunitX554111333ena859
15948Fusobacterium necrophorum 16S rRNA gene, strain ATCC 25286AJ8670391455ena859

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Fusobacterium necrophorum subsp. necrophorum ATCC 25286GCA_004006635completencbi143388
66792Fusobacterium necrophorum strain ATCC 25286859.47wgspatric859
66792Fusobacterium necrophorum subsp. necrophorum strain ATCC 25286143388.7completepatric143388
66792Fusobacterium necrophorum subsp. necrophorum strain NCTC13726143388.5wgspatric143388
66792Fusobacterium necrophorum ATCC 252862847466697completeimg859
66792Fusobacterium necrophorum necrophorum NCTC 137262859123070draftimg143388
66792Fusobacterium necrophorum ATCC 252862597490366draftimg859
67770Fusobacterium necrophorum ATCC 25286GCA_900104395scaffoldncbi859
67770Fusobacterium necrophorum subsp. necrophorum NCTC13726GCA_900451075contigncbi143388

GC content

@refGC-contentmethod
1594828thermal denaturation, midpoint method (Tm)
6777032.3thermal denaturation, midpoint method (Tm)

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
motileno92.174no
flagellatedno94.973no
gram-positiveno90.284no
anaerobicyes99.743yes
aerobicno98.365yes
halophileno81.166no
spore-formingno88.998no
thermophileno97.676no
glucose-utilyes83.279no
glucose-fermentyes57.071no

External links

@ref: 15948

culture collection no.: CCUG 4948, DSM 21784, ATCC 25286, CCUG 9994, CIP 104559, DSM 19788, JCM 3718, VPI 2891, BCRC 10679, NCTC 13726

straininfo link

  • @ref: 75239
  • straininfo: 38464

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny1883714Proposal of two subspecies of Fusobacterium necrophorum (Flugge) Moore and Holdeman: Fusobacterium necrophorum subsp. necrophorum subsp. nov., nom. rev. (ex Flugge 1886), and Fusobacterium necrophorum subsp. funduliforme subsp. nov., nom. rev. (ex Halle 1898).Shinjo T, Fujisawa T, Mitsuoka TInt J Syst Bacteriol10.1099/00207713-41-3-3951991Base Composition, DNA, Bacterial, Fusobacterium necrophorum/*classification/genetics/metabolism, Sequence Homology, Nucleic AcidMetabolism
Phylogeny2223600Recognition of biovar C of Fusobacterium necrophorum (Flugge) Moore and Holdeman as Fusobacterium pseudonecrophorum sp. nov., nom. rev. (ex Prevot 1940).Shinjo T, Hiraiwa K, Miyazato SInt J Syst Bacteriol10.1099/00207713-40-1-711990Animals, DNA, Bacterial/genetics, Fusobacterium Infections/microbiology, Fusobacterium necrophorum/*classification/genetics/metabolism/pathogenicity, Hemagglutination Tests, Liver Abscess/microbiology, Mice, Nucleic Acid Hybridization, Sequence Homology, Nucleic Acid, Species SpecificityMetabolism
Enzymology2643582Structural analysis of lipopolysaccharides from Eikenella corrodens by use of murine monoclonal antibodies.Kato T, Takazoe I, Okuda KInfect Immun10.1128/iai.57.2.656-659.19891989Animals, *Antibodies, Monoclonal, Antigen-Antibody Reactions, Antigens, Bacterial/immunology/*isolation & purification, Bacteroides/*immunology, Carbohydrate Conformation, Eikenella corrodens/*immunology, Female, Lipopolysaccharides/immunology/*isolation & purification, Mice, Mice, Inbred BALB CPhylogeny
Metabolism7476558Local skin reaction in mice and guinea pigs induced by a single intradermal inoculation of Fusobacterium necrophorum lipopolysaccharide.Kanoe M, Kiritani M, Inoue MMicrobios1995Animals, Dose-Response Relationship, Drug, Erythema/chemically induced, Escherichia coli/metabolism, *Fusobacterium necrophorum, Guinea Pigs, Hemorrhage/chemically induced, Inflammation/chemically induced, Injections, Intradermal, Lipopolysaccharides/*pharmacology, Mice, Mice, Inbred C3H, Mice, Inbred ICR, Salmonella typhimurium/metabolism, Skin/*drug effects/pathologyPathogenicity
Enzymology10347900Location of haemagglutinin in bacterial cells of Fusobacterium necrophorum subsp. necrophorum.Kanoe M, Koyanagi Y, Kondo C, Mamba K, Makita T, Kai KMicrobios1998Fluorescent Antibody Technique, Indirect, Fusobacterium necrophorum/*chemistry/pathogenicity/*ultrastructure, Hemagglutinins/*isolation & purification, Microscopy, ImmunoelectronPhylogeny
Metabolism10792652Adherence of Fusobacterium necrophorum subsp. necrophorum to ruminal cells derived from bovine rumenitis.Takayama Y, Kanoe M, Maeda K, Okada Y, Kai KLett Appl Microbiol10.1046/j.1472-765x.2000.00722.x2000Animals, *Bacterial Adhesion, Cattle, Cattle Diseases/*microbiology, Collagen/metabolism, Fluorescent Antibody Technique, Fusobacterium Infections/microbiology/*veterinary, Fusobacterium necrophorum/*physiology, Rumen/cytology/*microbiology, Stomach Diseases/microbiology/*veterinary
Enzymology16051369Fusobacterium necrophorum as the cause of recurrent sore throat: comparison of isolates from persistent sore throat syndrome and Lemierre's disease.Batty A, Wren MW, Gal MJ Infect10.1016/j.jinf.2004.09.0132004Adolescent, Adult, Bacteremia/*microbiology, Bacterial Typing Techniques/methods, Child, Child, Preschool, Electrophoresis, Agar Gel/methods, Fusobacterium Infections/*microbiology, Fusobacterium necrophorum/*classification/*isolation & purification, Humans, Infant, Infant, Newborn, Middle Aged, Pharyngitis/*microbiology, Pharynx/microbiology, Polymerase Chain Reaction/methods, Recurrence, SyndromePhylogeny
Metabolism18793368Stimulation of epithelial cell matrix metalloproteinase (MMP-2, -9, -13) and interleukin-8 secretion by fusobacteria.Gursoy UK, Kononen E, Uitto VJOral Microbiol Immunol10.1111/j.1399-302X.2008.00453.x2008Blotting, Western, Cell Line, Epithelial Cells, Fusobacterium/classification/*physiology, Fusobacterium necrophorum/physiology, Fusobacterium nucleatum/physiology, Humans, Interleukin-8/analysis/*metabolism, Keratinocytes/*enzymology/microbiology, Matrix Metalloproteinase 1/analysis/metabolism, Matrix Metalloproteinase 13/analysis/metabolism, Matrix Metalloproteinase 9/analysis/metabolism, Matrix Metalloproteinases/analysis/*metabolism, Mouth/microbiology, Time FactorsPathogenicity
Metabolism27966414[Design of artificial foetor flatus based on bacterial volatile compounds].Justesen USUgeskr LaegerV687072016Bacteroides fragilis/metabolism, Clostridioides difficile/metabolism, Clostridium perfringens/metabolism, Flatulence/metabolism/*microbiology, Fusobacterium necrophorum/metabolism, Humans, Odorants/*analysisPathogenicity
Genetics30834361Complete Genome Sequence of Fusobacterium necrophorum subsp. necrophorum ATCC 25286.Umana A, Lemkul JA, Slade DJMicrobiol Resour Announc10.1128/MRA.00025-192019
Pathogenicity32146452Chemical composition and antibacterial activity of essential oils against pathogens often related to cattle endometritis.Braga Paiano R, Bonilla J, Moro de Sousa RL, Micke Moreno A, Sampaio Baruselli PJ Infect Dev Ctries10.3855/jidc.120762020Actinomycetaceae/drug effects, Animals, Anti-Infective Agents/pharmacology, Bacterial Infections/drug therapy/*veterinary, Cattle, Cattle Diseases/*drug therapy/microbiology, Endometritis/drug therapy/microbiology/*veterinary, Escherichia coli/drug effects, Female, Fusobacterium necrophorum/drug effects, Gas Chromatography-Mass Spectrometry, Oils, Volatile/*chemistry/*pharmacology, Staphylococcus aureus/drug effects

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
15948Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 21784)https://www.dsmz.de/collection/catalogue/details/culture/DSM-21784
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
41710Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/16481
45248Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 9994)https://www.ccug.se/strain?id=9994
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68380Automatically annotated from API rID32A
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
75239Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID38464.1StrainInfo: A central database for resolving microbial strain identifiers
121252Curators of the CIPCollection of Institut Pasteur (CIP 104559)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20104559